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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Crop Science Jurnal Mandala Pengabdian Masyarakat
Wira Hadianto, Wira
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Public Health Social Sciences

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Early Identification of Potentially Drought-Tolerant Doubled Haploid Rice Lines During the Seedling Stage Munandar, Arief; Purwoko, Bambang Sapto; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi; Hadianto, Wira; Nurhidayah, Siti
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.346-357

Abstract

Climate change and reduced crop yields caused by drought stress have increased the demand for drought-tolerant varieties. The anther culture technique allows the production of improved varieties with high homozygosity in a short time. The study aimed to select the drought-tolerant double haploid lines at the seedling stage. This research was conducted in the greenhouse of the Indonesian Center for Agricultural Biotechnology and Genetic Resources Instrument Standard Testing (BBPSI Biogen), Bogor, from August to November 2023. The drought-tolerant selection of 12 doubled-haploid rice lines (AE1-AE12) and four check varieties, i.e., two commercial checks (“Inpari 18” Tadah Hujan Agritan or AE13, “Bioni63” Ciherang Agritan or AE14), one drought-tolerant check (“Salumpikit” or AE15), and one drought-sensitive check (“IR20” or AE16). The research used a randomized complete block design with three replications. The characters observed were leaf rolling, leaf drying, recovery ability, and plant fresh and dry weight. The Friedman test results showed that the lines with the lowest rankings, three lines (AE2, AE5, and AE12) with mild tolerant criteria for leaf rolling, five lines (AE1, AE2, AE5, AE8, and AE12) with mild tolerant criteria for leaf drying, and three lines (AE1, AE5, and AE12) with tolerant criteria for recovery ability. The selection index for drought tolerance at the seedling stage identified seven lines with positive values. Based on the Friedman test, selection index, and heatmap visualization, AE12, AE1, AE5, and AE8 exhibited a tolerance similar to “Salumpikit” and were deemed suitable based on drought tolerance characters.
Pemberdayan Melalui Pengembangan Potensi Lokal Berbasis Jeruk Nipis Menjadi Produk Turunan Guna Meningkatkan Perekonomian Masyarakat Desa Kuta Makmue Fajri, Maulidil; Putri, Nhyra Kamala; Itawarnemi, Hilmina; Hadianto, Wira; Ariska, Nana; Putra, Iwandikasyah; Latif, Abdul
Jurnal Mandala Pengabdian Masyarakat Vol. 6 No. 1 (2025): Jurnal Mandala Pengabdian Masyarakat
Publisher : Progran Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpm.v6i1.582

Abstract

Desa kuta makmue berlokasi di Kecamatan Kuala Kabupaten Nagan Raya merupakan salah satu desa dengan potensi pertanian yang tinggi, dengan komoditas utamanya jeruk nipis. Selama ini komoditas jeruk nipis hanya dimanfaatkan sebagai hasil bumi yang dijual dalam kondisi segar oleh masyarakat setempat tanpa diolah. Oleh karenanya perlu pengembangan Agroindustri lebih lanjut untuk mengelola sumber daya yang ada. Upaya pemberdayaan masyarakat agar mampu secara mandiri memanfaatkan potensi daerahnya salah satunya dengan pengetahuan dan keterampilan mengenai wirausaha dan teknologi tepat guna proses pembuatan sabun cuci piring dan sirup. Pendekatan yang diterapkan dalam merealisasikan program ini melalui metode participatory action research (PAR). Pelaksanaan kegiatan meliputi sosialisasi kewirausaahan dan penciptaan produk turunan, demonstrasi langsung pembuatan sabun cuci piring dan sirup jeruk nipis, pengemasan, hingga pemasaran.  Hasil menunjukkan bahwa kegiatan ini dapat (1) meningkatkan motivasi usaha, kesadaran, pengetahuan dan ketrampilan masyarakat melalui penerapan teknologi tepat guna untuk mengolah komoditas lokal (jeruk nipis) menjadi produk sabun cuci piring dan sirup jeruk nipis, (2) mengenalkan kepada masyarakat mengenai peluang dan analisis kelayakan usaha sabun cuci piring dan sirup rumahan. dan mampu menggerakkan produk inovasi di Desa Kuta Makmue.
Analysis of drought stress tolerance in doubled haploid lines of green super rice at the vegetative stage Nurhidayah, Siti; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi; Lubis, Iskandar; Hadianto, Wira; Munandar, Arief
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.63893

Abstract

Current climate change has caused drought in various regions, which has decreased rice yields. Green super rice (GSR) has the characteristic of being tolerant to water limitation. GSR lines were bred through anther culture technique to obtain doubled haploid (DH) plants. This study aimed to analyze the response of DH GSR lines to drought stress at the vegetative stage and identify potential lines based on the weighted selection index. The experiment was arranged in a randomized complete block design with three replications. The materials used consisted of twenty DH lines, Inpari 42 Agritan GSR, and Inpari 18 as check varieties, Salumpikit as a drought-tolerant check, and IR 20 as a drought-sensitive check. Qualitative data were analyzed using the non-parametric Friedman test. The result showed different responses among the lines for leaf rolling, leaf drying, and recovery ability. Line SN14 exhibited moderate leaf drying and showed improvement to mild tolerance during the recovery phase. Nine lines (SN12, 14, 32, 40, 51, 57, 58, 59, and 60) were selected using a selection index based on leaf rolling, leaf drying, and recovery ability. These lines can be further tested for drought tolerance tests until the reproductive stage, and the tolerant lines could be useful for future development. Keywords: abiotic stress; anther culture; climate change; selection index; water limitation
Co-Authors Abdul Latif Ariska, Nana Bambang Sapta Purwoko Fajri, Maulidil Iskandar Lubis Iswari Saraswati Dewi Itawarnemi, Hilmina Munandar, Arief Purwoko, Bambang Sapto Putra, Iwandikasyah Putri, Nhyra Kamala Siti Nurhidayah Willy Bayuardi Suwarno