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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Pendidikan dan Konseling
Priscinya Christiana Debora
Universitas Singaperbangsa Karawang
Education Health Professions Languange, Linguistic, Communication & Media Mathematics Social Sciences Other

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Artikel Review: Analisis Ekstraksi dan Identifikasi Senyawa Saponin pada Tumbuhan Mahkota Dewa (Phaleria macrocarpa) Saarah Hamidah Asmara Indratno; Priscinya Christiana Debora; Nur Komala Fitri; Tiwi Ambarati; Aliya Azkia Zahra; Vriezka Mierza
Jurnal Pendidikan dan Konseling (JPDK) Vol. 5 No. 1 (2023): Jurnal Pendidikan dan Konseling
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jpdk.v5i1.10948

Abstract

Mahkota dewa (Phaleria macrocarpa) adalah salah satu tanaman obat yang sering digunakan secara empiris oleh masyarakat di Indonesia karena kandungan metabolit sekunder didalamnya, salah satu senyawa tersebut adalah saponin. Metode yang dilakukan untuk menyusun artikel ini yaitu dengan studi literatur dari beberapa jurnal terakreditasi yang terbit selama sepuluh tahun terakhir. Pengujian kandungan senyawa saponin pada mahkota dewa (Phaleria macrocarpa) dilakukan dengan uji busa, dimana hasil positifnya menunjukan adanya busa stabil dengan tinggi 1-10 cm, yang pada saat ditambahkan HCl 2 N sebanyak 1 tetes busa tersebut akan tetap bertahan, sedangkan pada pengujian dengan menggunakan UV-Vis akan memberikan bercak orange kecoklatan setelah disemprotkan reagen Lieberman-Bourchat dan akan ada bercak pada sinar UV 254 nm dan sinar UV 366 nm. Lalu pada saponin steroid akan memberikan cincin hijau pada pengujian warna.
Molecular identification and antibacterial activity of endophytic bacteria from Bambusa vulgaris leaves as antibacterial potential against phatogenic microoganism Jekmal Malau; Vriezka Mierza; Munir Alinu Mulki; Lestari Mahardika Urbaningrum; Vina Luthfiana Hasna; Priscinya Christiana Debora
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i2.6538

Abstract

The increasing prevalence of antibiotic-resistant bacteria poses a critical global health concern, necessitating the exploration of novel antibacterial solutions. Endophytic bacteria, which colonize plant tissues without causing harm, have gained attention as potential sources of bioactive metabolites. This study aimed to isolate and characterize endophytic bacteria from Bambusa vulgaris leaves and evaluate their antibacterial potential against pathogenic microorganisms. Leaf samples were collected from Bekasi, West Java, and subjected to a surface sterilization process prior to bacterial isolation. A total of 12 bacterial strains were successfully obtained and screened for antibacterial activity against Bacillus subtilis, Propionibacterium acnes, Pseudomonas aeruginosa, and Staphylococcus epidermidis using the agar well diffusion assay. Three isolates exhibited notable inhibitory activity, with P8 demonstrating the strongest antibacterial effects against B. subtilis, P. acnes, and S. epidermidis. The two most potent isolates, P8 and K3, were characterized via 16S rRNA gene sequencing. Genomic DNA extraction was performed, followed by Polymerase Chain Reaction (PCR) amplification using the universal primers 27F (5′-AGAGTTTGATYMTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTACGACTT-3′). Sequencing and The Basic Local Alignment Search Tool (BLAST) analysis confirmed that isolate P8 exhibited 100% similarity to B. subtilis strain LZH-H1, whereas isolate K3 shared 99.85% similarity with Pantoea stewartii subsp. indologenes strain SR2-12. These findings suggest that endophytic bacteria from B. vulgaris endohytic bacteria hold promise as potential sources of antibacterial compounds. Further research is necessary to purify and characterize these bioactive metabolites for potential pharmaceutical applications.
Co-Authors Aliya Azkia Zahra Jekmal Malau Lestari Mahardika Urbaningrum Munir Alinu Mulki Nur Komala Fitri Saarah Hamidah Asmara Indratno Tiwi Ambarati Vina Luthfiana Hasna Vriezka Mierza