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PEMERIKSAAN JUMLAH KOLONI MIKROORGANISME DI UDARA PADA PEMUKIMAN WARGA DI SEKITAR TEMPAT PEMBUANGAN AKHIR SAMPAH TERJUN Suharti, Nin; P., Halimah Fitrian
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Vol 13 No 2 (2018): Jurnal Ilmiah PANNMED Periode September-Desember 2018
Publisher : Politeknik Kesehatan Kemetrian Kesehatan Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.556 KB)

Keterbatasan tempat tinggal di daerah perkotaan semakin bertambah dari waktu ke waktu, karenapertumbuhan penduduk lebih cepat dibandingkan dengan ketersediaan lahan. Kondisi inimengakibatkan munculnya permasalahan perumahan yang semakin sulit di perkotaan.Untukmengatasi kebutuhan perumahan mereka cenderung tinggal di daerah pinggiran, termasuk masyarakatumum dan pemulung yang bermukim di sekitar lokasi tempat pembuangan akhir sampah (TPAS).Secara khusus penelitian ini bertujuan untuk mengetahuiJumlah koloni mikroorganisme di udara padapemukiman warga sekitar TPAS Terjun. Kegiatan dilakukan di TPAS dan sekitarnya. Jumlahmikroorganisme yang direkomendasikan oleh Menteri Kesehatan RI No:1077/MENKES/PER/V/2011mengenai udara yang sehat adalah jumlah mikroorganisme < 700 CFU / m3 udara. Penelitian inidilaksanakan pada bulan Juli s/d Agustus 2018 bertujuan untuk mengetahui jumlah mikroorganismeudara pada lokasi tempat pembuangan akhir sampah (TPAS) Terjun Medan dan tempat tinggalmasyarakat di sekitarnya. Metode yang digunakan pada penelitian ini adalah Experimen menggunakanCulture pada media Plate Cout Agar (PCA) dan alat Air Exampler Mas 100. Hasil penelitianmenunjukkan bahwa pada titik pusat TPAS (0 meter) jumlah mikroorganisme udara sebanyak 2874cfu/m3, 100 m dari titik pusat TPAS Jumlah Mikroorganisme 2294 cfu/m3,250 m dari titik pusatTPAS Jumlah Mikroorganisme 1913 cfu/m3, 500 m dari titik pusat TPAS Jumlah Mikroorganisme966 cfu/m3,750 m dari titik pusat TPAS Jumlah Mikroorganisme 662 cfu/m3, 1000 m dari titik pusatTPAS Jumlah Mikroorganisme 517 cfu/m3 Semakin jauh dari titik pusat jumlah mikroorganismeudara semakin menurun dengan angka korelasi 95,7% dan jarak yang aman bagi masyarakatadalah ≥750 meter, karena populasi mikroorganisme udara sudah dibawah standar baku mutu yangditetapkan (<700 cfu/m3).

HUBUNGAN ANTARA POPULASI MIKROORGANISME UDARA DENGAN KEJADIAN INFEKSI SALURAN PERNAFASAN AKUT DI SEKITAR TEMPAT PEMBUANGAN AKHIR SAMPAH TERJUN MEDAN Nin Suharti; Erman Munir; Dwi Suryanto; Harry Agusnar
Jurnal Pendidikan Kimia (JPKim) Vol 6, No 1 (2014): April
Publisher : Pascasarjana Universitas Negeri Medan

Abstract. This study aims to determine the number of microorganisms in the landfill Terjun Medan and to determine its relationship with acute respiratory infections in the surrounding community. The results showed that at the center of TPAS (0 meter) the number of air microorganisms was 2322 cfu/m3. The farther from the center point the amount of air microorganisms declined with 94.4 % correlation rate. A safe distance was ≥ 750 meters, because the microorganism population was below the air quality standards established (<700 cfu/m3). Analysis of the incidence of acute respiratory infections in the community around landfill Terjun Medan indicated that the higher the population of air microorganisms the higher acute respiratory infections event with correlation value of 85.7 %. Identification of air microorganisms showed that the air around the landfill Terjun Medan contained acute respiratory infections causing microbes consisting of Streptococcus, Staphylococcus, Klebsiella, Corynebacterium, Aspergillus and Candida. Keywords : Landfill, Air Microorganisms, Acute Respiratory Infections

UJI EFEKTIVITAS EKSTRAK DAUN BINAHONG (Anredera Cordifolia) TERHADAP PERTUMBUHAN BAKTERI STAPHYLOCOCCUS AUREUS Nin Suharti
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Vol. 17 No. 3 (2022): Jurnal Ilmiah PANNMED Periode September- Desember 2022
Publisher : Poltekkes Kemenkes Medan

Binahong is one of the plants that can treat various types of diseases, especially infectious diseases. Infectious diseases are the main cause of high morbidity and mortality, especially in developing countries such as Indonesia and the most common is Staphylococcus aureus. Binahong plant (Anredera cordifolia) is a potential medicinal plant that can overcome various types of diseases and has inhibitory power against bacteria. This study aims to determine the effectiveness of binahong leaf extract against the growth of Staphylococcus aureus bacteria by measuring the inhibition zone formed. The materials used are binahong leaf extract and Staphylococcus aureus bacteria. This type of research is experimental using disc paper with levels of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%. In Muller Hinton media for the growth of Staphylococcus aureus bacteria, the diameter of the inhibition zone formed was measured. The results showed that the highest value of the inhibition zone of the binahong leaf extract was at a concentration of 80% with an inhibitory zone of 9.78 and the lowest was at a concentration of 10% with an inhibitory zone of 8.63. The effectiveness of the best binahong leaf extract in inhibiting the growth of Staphylococcus aureus bacteria is at a concentration of 80%. This indicates that the binahong leaf extract has an antibacterial effect against Staphylococcus aureus bacteria.Abstrak

ISOLASI DAN IDENTIFIKASI BAKTERI TERMOFILIK PENGHASIL AMILASE DARI SUMBER AIR PANAS SIDEBUK-DEBUK SUMATERA UTARA Nin Suharti Suharti; Geminsah Putra
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Vol. 17 No. 1 (2022): Jurnal Ilmiah PANNMED Periode januari -April 2022
Publisher : Poltekkes Kemenkes Medan

Hot springs or hot springs are springs that are produced as a result of the release of groundwater from the earth's crust after experiencing geothermal heating. Indonesia is one of the most active tectonic regions in the world with more than 70 active volcanoes, and has many geothermal areas. Hot springs are a suitable growth medium for thermophilic bacteria. Thermophilic bacteria are a group of bacteria that adapt to high-temperature environmental conditions, with temperatures ranging from 50°C-75°C. The natural habitat of thermophilic bacteria is widespread throughout the earth's surface, including in hot springs, volcanic craters or volcanic areas. One of the hot springs in North Sumatra province is sidebuk-debuk, which is located in Bandar Baru sub-district. is a hot spring, which has a temperature between 50°C – 75°C. At this time thermophilic bacteria are studied and researched intensively for reasons of developing basic research and biotechnology applications. Thermophilic bacteria have the potential to produce heat-resistant/ thermostable enzymes and can be used in industry, waste treatment processes and mineral weathering. Amylase is an important enzyme that is widely used in industrial applications through synthesis reactions and hydrolysis reactions, accounting for almost 65% of the total sales of enzymes in the world. Researchers are interested in knowing more about the role of thermostable enzymes in modern biotechnology today, therefore it is necessary to conduct research aimed at isolating and identifying local isolates of thermophilic bacteria as potential sources of enzymes, especially in North Sumatra. From this study, 19 isolates of Bacillus sp bacteria were obtained from Sidebuk-debuk hot springs based on phenotypic characters by looking at macroscopic, microscopic and biochemical characteristics, with 9 isolates of thermophilic bacteria that have the potential to produce amylase as indicated by the presence of a clear zone around the bacterial colonies.Keywords: Hot Springs, Bacillus sp Bacteria,

Pemanfaatan Bakteri Termofilik Penghasil Amilase Pada Berbagai Pati Nin Suharti; Geminsah Putra
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Vol. 18 No. 2 (2023): Jurnal Ilmiah PANNMED Periode Mei - Agustus 2023
Publisher : Poltekkes Kemenkes Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36911/pannmed.v18i2.1594

Indonesia is a country that produces a lot of starchy materials such as cassava, sago, corn and others. Indonesia has the potential to develop an enzyme-producing industry, especially amylase. The utilization of starchy materials which so far has not been maximized and is still limited to conventional methods can be increased by enzymatic development processes. This will increase its economic value and at the same time increase state income. One of the enzymes that is currently very widely used in the food and beverage industry is amylase. Nin Suharti and Geminsyah Putra 2021 have succeeded in isolating and identifying thermophilic bacteria from the sidedust hot springs of North Sumatra. The advantage of thermophilic bacteria is that they can produce enzymes that are resistant to high temperatures / thermostable enzymes. These enzymes are able to survive and are active at high temperatures. Such properties are highly needed by enzyme-based industries. The use of enzymes that can withstand high temperatures in the field of biotechnology can reduce operating costs and increase reaction speed, this study uses various types of starch including potatoes, cassava and corn as suitable and easily available carbon sources for the production of amylase enzymes. Bacteria in the media with potato starch added can produce the highest amylase enzyme from the three treatments of 65 U/L. For bacteria added with corn starch, the result was 50 U/L of amylase enzyme. Meanwhile, the lowest of the three treatments was media with added sweet potato starch with a content of 39 U/L. The isolates generally had a round shape, jagged edges, grooved, and smooth, white colonies, microscopic bacilli-shaped, endospores and motile, positive for catalase, gelatin and citrate tests. With Gram staining, it generally shows a positive Gram reaction.

Investigating Husband's Support and Murrotal Therapy Effects on Maternal Anxiety in Childbirth Sri Wahyuni; Nin Suharti
Contagion: Scientific Periodical Journal of Public Health and Coastal Health Vol 6, No 1 (2024): CONTAGION
Publisher : Universitas Islam Negeri Sumatera Utara, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30829/contagion.v6i1.17774

First-time mothers may find childbirth difficult; they frequently feel intense anxiety, stress, and terror as the due date draws near, particularly in the third trimester of pregnancy when there is a higher chance of an early birth. The aim of the research was to ascertain the impact of Murottal therapy and husband support on the anxiety levels of expectant mothers during labor at Badrul Aini Mother and Child Hospital. This study uses a cross-sectional research approach and is quantitatively analytical. In August 2023, the study was carried out at the Badrul Ain Mother and Child Hospital. All of the pregnant patients in this study came to the Badrul Aini Mother and Child Hospital to give birth. The sampling technique was random, so the research sample was obtained from as many as 50 pregnant women. Data collection techniques in this study used questionnaires and observation techniques. Data analysis used correlation and multiple linear regression tests with SPSS software processing. The results showed that husband support (p-value = 0.006) and murrotal therapy significantly reduced the anxiety level of pregnant women (p-value = 0.001). However, the hypothesis that Murrotal therapy moderates the effect of husband support on anxiety levels was not supported. The importance of the husband's role in providing emotional and psychological support during labor and confirming the effectiveness of Murrotal therapy in managing maternal anxiety. Healthcare providers should consider involving husbands in prenatal care and labour, and Murrotal therapy can be a valuable tool in managing pregnant women's anxiety. Keywords: Childbirth, Husband's Support, Murrotal, Therapy, Pregnant mother's anxiety

ISOLASI DAN PEMANFAATAN BAKTERI KITINOLITIK DARI CANGKANG UDANG PUTIH (PENAEUS MERGUENSIS) DALAM MENGHASILKAN ENZIM KITINASE Nin Suharti; Halimah Fitriani
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Vol. 18 No. 3 (2023): Jurnal Ilmiah PANNMED Periode September- Desember 2023
Publisher : Poltekkes Kemenkes Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36911/pannmed.v18i3.1703

Indonesia is listed as the third largest shrimp producing country in the world. Shrimp is one of the leading export commodities. Shrimp is one of the main commodities in the fishing industry. Thus encouraging Indonesia to become one of the shrimp producing countries. In the shrimp processing process, usually the heads and shells of the shrimp are thrown away or used as animal feed. Traditionally, shrimp shell waste is generally used as a mixture for making crackers, paste, shrimp paste and as additional animal feed and returned to the shrimp cultivation itself. One use of shrimp shell waste that has prospects for development and has quite high economic value is processing it into chitin, chitosan and glucosamine. The aim of this research is to obtain isolates of chitinolytic bacteria from isolation and to utilize chitinolytic bacteria from white shrimp (Penaeus merguensis) shells to produce chitinase enzymes. Chitinolytic bacteria are bacteria that produce chitinase enzymes which play a role in degrading chitin into oligomers and chitin derivative compounds. Chitinolytic bacteria can be obtained from various sources such as the rhizosphere, phyllosphere, soil or from water environments such as seas, lakes, ponds or shrimp ponds and so on. Chitin that exists in nature is easily degraded by microorganisms. There are two pathways in the chitin degradation process. First, degradation by chitinolytic which hydrolyzes the ?-1,4-glycoside bond. Second, the polymer undergoes first deacetylation and then hydrolysis by chitosanase. Chitinase has many benefits, including as a bioinsecticide and biofunctional agent in controlling plant pests and is used to process chitin waste in the shrimp and crab freezing industry. It can also be used in the medical and food fields. From the test results, three types of bacteria were obtained, namely Vibrio sp, Bacillus sp, and Pseudomonas sp.

Pemanfaatan Bakteri Kitinolitik dari Limbah Kulit Udang Sebagai Biopestisida Berkelanjutan terhadap Drosophila Sp. dalam Sistem Hortikultura Nin Suharti Hasibuan; Nelma Nelma; Suryani Mf. Situmeang; Suliati Suliati
Jurnal Biologi Vol. 3 No. 1 (2025): November
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/biology.v3i1.5018

Penelitian ini bertujuan untuk mengevaluasi potensi enzim kitinase yang dihasilkan oleh bakteri kitinolitik dari limbah kulit udang sebagai biopestisida hayati terhadap larva lalat buah (Drosophila sp.). Penelitian dilakukan melalui tiga tahap, yaitu isolasi dan identifikasi bakteri kitinolitik, pengujian aktivitas enzim kitinase, serta uji efektivitas enzim terhadap kematian larva. Sampel kulit udang putih (Penaeus merguiensis) digunakan sebagai sumber isolat alami yang kaya akan kitin. Hasil isolasi menghasilkan tiga jenis bakteri, yaitu Vibrio sp., Bacillus sp., dan Pseudomonas sp., yang semuanya menunjukkan aktivitas kitinolitik dengan terbentuknya zona bening pada media kitin. Identifikasi morfologi dan biokimia memperkuat hasil tersebut melalui uji pewarnaan Gram, motilitas, serta serangkaian uji fermentasi gula dan TSIA. Uji efektivitas menunjukkan bahwa filtrat enzim kitinase dengan konsentrasi di atas 50% menyebabkan kematian larva secara signifikan, dengan waktu tercepat terjadi pada konsentrasi 100%, yaitu 10 menit 15 detik, sedangkan pada konsentrasi di bawah 40% tidak menunjukkan efek mematikan. Aktivitas enzim kitinase bekerja dengan mendegradasi komponen kitin pada eksoskeleton larva, menyebabkan kerusakan struktural dan gangguan fisiologis yang berujung pada kematian. Hasil penelitian ini menegaskan bahwa enzim kitinase memiliki potensi besar sebagai agen biopestisida ramah lingkungan yang efektif, ekonomis, dan berkelanjutan. Selain itu, pemanfaatan limbah kulit udang sebagai sumber bioteknologi terapan tidak hanya memberikan solusi terhadap permasalahan limbah industri perikanan, tetapi juga mendukung prinsip ekonomi sirkular, inovasi hijau, serta penerapan pertanian hortikultura yang lebih aman bagi manusia, ekosistem, dan keberlanjutan lingkungan jangka panjang.

Evaluasi Tepung dan Infusum Kacang Hijau (Vigna radiata) sebagai Media Alternatif Pertumbuhan Staphylococcus aureus Febri Sembiring; Suparni; Dewi Setiyawati; Wardati Humaira; Sri Widia Ningsih; Nin Suharti; Astrid Siska Pratiwi
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 2 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i2.727

Limited access to and the high cost of commercial growth media have driven the development of alternative media based on locally available materials. Although several legume-based materials have been reported to support bacterial growth, the use of mung beans (Vigna radiata) as an alternative medium—particularly in the form of flour and infusion—and comparisons of their effectiveness against standard media remain limited. This study aims to evaluate the potential of these two forms as growth media for Staphylococcus aureus. This was an experimental study conducted from March to May 2024 at the Microbiology Laboratory of the Medan Public Health Polytechnic (Poltekkes Kemenkes). The method used was growth analysis via the Total Plate Count (TPC) method. The results showed that the average TPC of S. aureus on mung bean flour medium was 1.07 × 10⁷ CFU/mL, and on mung bean infusion medium it was 4.7 × 10⁶ CFU/mL, whereas TSA medium, as the standard medium, yielded 5.8Z× 10⁶ CFU/mL. This indicates that both forms of the mung bean alternative media were capable of supporting optimal bacterial growth, with the flour medium yielding a higher cell count compared to the standard medium (TSA). Morphologically, the colonies on the alternative media were smaller (±2 mm, yellowish-white) compared to those on TSA (±3 mm, yellow). Thus, it can be concluded that mung bean flour has the potential to be an effective alternative medium for growing S. aureus compared to the infusion medium, which is less optimal due to the possible degradation of nutrients during the heating process.

EVALUASI EFEKTIVITAS HEMODIALISIS PADA PASIEN GAGAL GINJAL KRONIS DI RSUD H. ABDUL MANAN SIMATUPANG KISARAN Nelma; Nin Suharti; Zansisco Rogabe Hamonangan Pardosi
Jurnal Ilmiah PANNMED (Pharmacist, Analyst, Nurse, Nutrition, Midwivery, Environment, Dentist) Vol. 21 No. 2 (2026): Jurnal Ilmiah PANNMED Periode Mei - Agustus 2026
Publisher : Poltekkes Kemenkes Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36911/pannmed.v21i2.2632

Chronic kidney disease (CKD) is a disease characterized by a progressive decline in kidney function, often accompanied by anemia due to low hemoglobin (Hb) levels. Hemodialysis is a therapy used to replace kidney function and has the potential to affect Hb levels. This study aims to determine changes in hemoglobin levels before and after hemodialysis in CKD patients. The study was conducted at H. Abdul Manan Simatupang Kisaran Regional Hospital with a quantitative design to observe hemoglobin levels before and after hemodialysis. A total of 35 CKD patients undergoing hemodialysis were taken as samples using a purposive sampling technique with certain inclusion criteria. Hb level data were collected from medical records and analyzed using a paired t-test. The results showed that Hb levels before hemodialysis had an average of 8.217 g/dL and increased to 8.831 g/dL after hemodialysis. The statistical test showed a p value = 0.000 (p < 0.05), which means there was a significant difference between Hb levels before and after hemodialysis. The conclusion of this study is that hemodialysis has a significant effect on increasing hemoglobin levels in patients with chronic kidney failure.

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