Sugiah Sugiah
Prodi DIII Analis Kesehatan, STIKes Karsa Husada Garut, Garut, Indonesia

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Isolation and identification of escherichia coli in well water located in Garut Regency Sugiah Sugiah; Gina Nafsa Mutmaina; Mamay Mamay; Astari Nurisani
Science Midwifery Vol 11 No 1 (2023): April: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i1.1236

Abstract

Water is one of the most important needs for daily life, especially well water. In the Garut district area well water is used for daily activities ranging from washing, drinking, bathing, ablution to being used for drinking water consumption. This study aims to isolate and identify well water from 10 villages located in the Garut Regency. Sampling was done using purposive sampling method. The test was followed by a presumptive test using Lactose Broth (LB), a reinforcing test using 2% BGLB media and a complementary test consisting of EMB agar media and biochemical tests. The results showed that of the 10 well water samples, 7 met the requirements of proper water quality and 3 did not meet the requirements of clean water with MPN values from three villages, namely Kampung Tanjung kamuning 200/ml, Kampung salaawi 241/100ml and Kampung Cipepe 241/100ml. EMB agar media is characterized by the formation of a metallic green color with black spots colonies and biochemical tests that show the well water has been contaminated with Escherichia coli bacteria.
Overview of Triglyceride levels in Lipemic Serum using Polyethylene Glycol (PEG) 6000 Sugiah Sugiah; Kurniawan Dewi Budiarti; Astari Nurisani; Gina Nafsa Mutmaina; Alya NSY
Journal of Midwifery and Nursing Vol. 5 No. 3 (2023): September: Health Science
Publisher : Institute Of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/jmn.v5i3.4187

Abstract

Lipemic causes interference with wavelength and light scattering caused by the presence of lipid particles. This study aims to determine the description of triglyceride levels in lipemic serum with and without the addition of polyethylene glycol (PEG) 6000. This type of research is experimental. The research conducted was descriptive research. The samples used were lipemic serum totaling 30 samples with triglyceride levels above 300 mg/dl. The results of the data analysis showed a mild lipemic level of 22 samples with an average of 379.00 mg/dl, a moderate lipemic level of 5 samples with an average of 558.40 mg/dl, and a severe lipemic serum of 3 samples with an average of 943.40 mg/dl. Based on the results of triglyceride levels without the addition of PEG 6000, the average level is 465.33 mg/dl, while the results of triglyceride levels using PEG 6000, the average level is 243.23 mg/dl. Based on the difference in triglyceride levels before and after adding PEG 6000, namely at the mild level, the average is 215.00 mg/dl (56%), at the moderate level, the average is 261.00 mg/dl (46%), and at the severe level, the average is 212.30 mg/dl (22%). It can be concluded that there are differences in triglyceride levels in lipemic serum before and after adding PEG 6000.
Antibiotics And Bacterial Resistance: A Global Challenge That Needs To Be Addressed Sugiah Sugiah; Fadli Fadli; Fitria Hariati; Khrisna Agung Cendekiawan
Journal of Pharmacopoeia Vol. 2 No. 1 (2025): MARCH-JP
Publisher : Pt. Anagata Sembagi Education

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62872/mwb7an91

Abstract

This study aims to explore the global challenges faced in addressing bacterial resistance to antibiotics, which are now one of the most serious health threats in the world. This study was conducted through the Systematic Literature Review (SLR) method by searching scientific articles from various databases such as PubMed, Scopus, and Google Scholar using the keywords "antibiotic resistance", "global health", "treatment failure", and "resistance mechanisms". The articles analyzed are publications in the last 10 years, focusing on the causes of resistance, their impact on global health, and countermeasures that have been carried out. The results of the study show that antibiotic resistance is mainly caused by the inappropriate use of antibiotics in the medical and non-medical sectors, lack of distribution supervision, and lack of development of new antibiotics. To overcome this, a comprehensive approach is needed, including increasing public education and health workers, strengthening regulations, and developing alternative therapies such as phagotherapy and vaccines. Without proper treatment, antibiotic resistance can lead to infection treatment failure, increased mortality rates, and burden the global health system