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All Journal Klinikal Sains (Jurnal Analis Kesehatan) JURNAL KESEHATAN PERINTIS INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Medika Cendikia (e-Journal) Jurnal Ilmiah Farmako Bahari Science Midwifery Jurnal Sains dan Teknologi Laboratorium Medik Jurnal Kesehatan Jurnal Abdi Masyarakat Kita Ekalaya: Jurnal Pengabdian Kepada Masyarakat Indonesia Mukhtabar: Journal of Medical Laboratory Technology Jurnal Ilmiah Ilmu Kesehatan
Mamay
Department Of Medical Laboratory Technology, STIKes Karsa Husada, Garut, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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UTILIZATION OF 1% OF METHYLENE BLUE IN STAINING HISTOPATHOLOGICAL PREPARATIONS AT ANATOMIC PATHOLOGY LABORATORY Tri Rahmawati; Yadi Apriyadi; Mamay
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i2.1563

Abstract

Tissue staining using hematoxylin-eosin (HE) is a standard method of histopathological staining. The tissue staining is hampered when there is no hematoxylin reagent in laboratory. Therefore, other reagents are needed that can replace the use of hematoxylin. Methylene blue is a basic dyes that interact with cell nuclei which has a negative ionic charge of the tissue. It can be used as an alternative nuclei staining. This study aims to evaluate the use of 1% of methylene blue in cell nuclei staining in histopathological preparations. The research sample were 15 pathology preparations which were randomly selected including breast cancer, cervical cancer and ovarian cancer in the bank of sampel at anatomical pathology laboratory of RSUD Dr. Slamet Garut, Indonesia. The experiment showed that the methylene blue dyes yielded “worth” result (40%) and “poorly” result (60%). Further research can be carried out by modifying the pH of 1% of methylene blue reagent so that it can maximize the staining preparations results as good as those using hematoxylin.
ANALISIS KANDUNGAN BORAKS PADA PEMPEK YANG DIJUAL DI KECAMATAN GARUT KOTA Mamay Mamay; Muhammad Hadi Sulhan
Jurnal Sains dan Teknologi Laboratorium Medik Vol 6 No 1 (2021): April (2021)
Publisher : Akademi Kesehatan John Paul II Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52071/jstlm.v6i1.62

Abstract

Pempek merupakan salah satu makanan tradisional ciri khas Palembang. Pempek sudah dikenal masyarakat secara luas. Masyarakat tidak terlalu susah untuk mendapatkan pempek di jajanan di sekitaran Garut Kota. Pembuatan pempek ini meggunakan bahan dasar utama daging ikan dan sagu. Beberapa temuan mengenai penggunaan boraks pada pempek di beberapa tempat. Penelitian bertujuan untuk mengidentifikasi dan menganalisis penggunaan borak pada empek yang dijual di sekitar kecamatan Garut Kota. Penelitian ini merupakan studi deskriptif dan laboratorium dengan tujuan untuk mengidentifikasi dan melakukan penetapan kadar boraks menggunakan analisis kualitatif dengan kurkumin dan analisis kuantitatif dengan titrasi asam basa. Sampel pempek yang peroleh dari 20 pedagang yang terseber di kecamatan Garut Kota. Berdasarkan hasil penelitian, secara kualitatif teridentifikasi 3 sampel pempek yang positif mengandung boraks. Hasil dari pemeriksaan organoleptis dari 20 sampel pempek, 3 sampel mempunyai tekstur yang sangat kenyal dan berbau seperti zat kimia sedangkan pada sampel pempek tidak mengandung boraks. Pengujian boraks dilanjutkan pada penetapan kadar atau analisa kuantitatif. Kandungan boraks pada ketiga sampel adalah 120 mg/g, 77,1 mg/g, dan 40,9 mg/g Dari hasil tersebut, disarankan agar masyarakat bisa membedakan dan memilih jajan pempek yang tidak mengandung boraks.
PENGGUNAAN EKSTRAK KAYU SECANG DAN KOL UNGU PADA MEDIA MANITOL SALT AGAR UNTUK MENUMBUHKAN Staphylococcus Mamay mamay
Klinikal Sains : Jurnal Analis Kesehatan Vol 10 No 1 (2022): Juni
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v10i1.2528

Abstract

Mannitol Salt Agar (MSA) is a selective and differential medium for isolating Stapylococcus bacteria, distinguishing Staphylococcus aureus bacteria from other species from the ability of mannitol to ferment which changes the color of the phenol red indicator from red to yellow. The use of synthetic indicators that are not environmentally friendly and difficult to decompose can be replaced with natural indicators, including the use of secang wood extract (Caesalpinia sappan L.) and purple cabbage (Brasicca oleraceae). The research was conducted using a descriptive method which describes the color changes of the modified mannitol salt agar (MSA) media of secang wood and purple cabbage extract after being planted with bacteria. Staphylococcus aureus and Coagulation Negative Staphylococcus (CoNS) bacteria were isolated from acne-prone skin. The growth of Staphylococus aureus changed the color of the modified MSA medium from red to yellow for the secang wood extract and the color change from blue to pink for the purple cabbage extract. The growth of CoNS bacteria changed the color of the modified MSA medium from red to red-orange for the secang wood extract and the color change from blue to blue-purple for the purple cabbage extract. Both bacteria thrived on purple cabbage modified MSA media. From this study, it was concluded that secang wood extract and purple cabbage could be used as natural indicators of modified MSA in growing Staphylococcus isolated from the skin.
Aktivitas Antioksidan Total pada Ekstrak Etanol Daun Bambu Surat (Gigantochloa pseudoarundinaceae) Mamay Mamay; Diah Wardani; Fathul Hakim
JURNAL KESEHATAN PERINTIS Vol 9 No 1 (2022): Jurnal Kesehatan Perintis
Publisher : LPPM UNIVERSITAS PERINTIS INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33653/jkp.v9i1.797

Abstract

Antioksidan merupakan senyawa yang dapat menghambat reaksi oksidasi, dengan cara mengikat radikal bebas dan molekul yang sangat reaktif dalam tubuh manusia Bambu surat (G. pseudoarundinaceae) adalah salah satu jenis tanaman bambu epidemik Indonesia yang diketahui mengandung senyawa antioksidan. Tujuan dari penelitian ini adalah untuk mengetahui gambaran aktivitas antioksidan total dalam ekstrak etanol daun muda, tua dan sangat tua bambu surat, dengan metode spektrofotometri. Jenis penelitian yang digunakan adalah deskriptif laboratorik dengan pemeriksaan laboratorium secara kualitatif dan kuantitatif. Sampel yang diambil dalam penelitian ini yaitu daun muda, tua dan sangat tua. Pengambilan sampel dilakukan dengan teknik purposive sampling. Pengolahan sampel dengan cara ekstraksi dengan metode meserasi cara dingin menggunakan etanol. Penetapan kadar antioksidan dilakukan dengan menggunakan spektrofotometri dan data yang didapat diolah dengan perhitungan persentasi inhibisi, IC50 (inhibitory concentration), AAI (antioxidant activity index). Pada ekstrak daun muda memiliki nilai IC50 57,46 ppm, AAI 2.78; dan pada ekstrak daun tua memiliki nilai IC50 42,02 ppm dan AAI 3.81 (AAI>2 antioksidan bersifat sangat kuat). Sedangkan ekstrak daun sangat tua memiliki nilai IC50 123,80 ppm dan AAI 1.29 (AAI>I antioksidan bersifat kuat. Lebih lanjut, Kuercetin dan vitamin C sebagai pembanding memiliki nilai IC50 dan AAI sebesar 33,74 (AAI=4,74) dan 37,91 (AAI=4,22).
Identifikasi dan Penetapan Kadar Rhodamin B pada Terasi yang Dijual di Pasar Ciawitali Kabupaten Garu Mamay Mamay; Acep Gunawan
Jurnal Medika Cendikia Vol 4 No 02 (2017): JURNAL MEDIKA CENDIKIA
Publisher : STIKes Karsa Husada Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Some synthetic dyes increasingly used by public along with the development of science and technology. Rhodamin B is one of synthetic dyes commonly used in textile and paper industry. However, the dye is often misused in food use. According to the Regulation of Minister of Health No. 33 of 2012 states that Rhodamin B is a prohibited Food Additives. Terasi is a food wich made from shrimp that is often found in the market by using red food color additives. The worries of using Rhodamine B used in terasi wich sold in Ciawitali Market of Garut Regency are the background of this research. Therefore, identification and determination of Rhodamine B on terasi wich sold in those markets. A laboratory descriptive method was used in this study, which illustrated the presence and Rhodamine B content in the terasi samples. To identify the presence of Rhodamin B contained in terasi, a qualitative test was used thin layer chromatography (TLC) method. To determine concentration of Rhodamin B in terasi, a quantitative test used UV-Vis Spectrophotometry with wavelength 554 nm. From the research results obtained from five samples of terasi found four samples of red terasi contain Rhodamin B. The value of Rhodamine B in samples 1 to 4 each as much as 222.5 mg / kg; 201.9 mg / kg; 57.55 mg / kg and 72.6 mg / kg. Based on the results of this study, red terasi wich sold on the market Ciawitali contain Rhodhamin B with various levels and suggested to the public should be careful to buy the red terasi.
Edukasi Dan Pemeriksaan Boraks Dengan Bahan Alami Pada Jajanan Sekolah Menengah Atas Mamay Mamay; Muhammad Hadi Sulhan; Riki Ananda
Jurnal Abdi Masyarakat Kita Vol 2 No 2 (2022): Jurnal Abdi Masyarakat Kita
Publisher : APDFI (Asosiasi Pendidikan Diploma Farmasi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33759/asta.v2i2.292

Abstract

Borax is a food additive which is prohibited from being used in food. Toxic effects of borax have been found to be harmful to human health. This compound is still widely abused in food samples. Detection of the presence of borax in the sample can be done with the use of natural materials. This community service aims to provide education that can increase the understanding of high school student participants about the dangers of borax and the identification with natural indicator such as turmeric (Curcuma longa), azalea flowers (Rhododendron concina) and red soka flower (Ixora auminta). The method used in the form of counseling and training on borax detection in samples with test paper from turmeric extract, azalea flower extract and red soka flower extract. In the implementation of the service, it was found that there was a change in understanding of borax and its toxic effects from 58% to 96% of science class students and 27% to 87% of social studies students. In the borax examination training, 92% of science student participants and 88% of social studies students were able to perform an examination with natural ingredients using test paper of turmeric extract, azalea flower, red soka flower. From the service process, it was found that, most of the participants knew about borax as a additive food which is prohibited from using and is harmful to health and can carry out a borax examination using natural indicators from turmeric, azalea flowers and red soka flowers.
PENYULUHAN DAN PEMBUATAN SUPLEMEN MAKANAN DARI TANAMAN OBAT KELUARGA (TOGA) UNTUK MENURUNKAN STUNTING DI DESA SINDANG MEKAR WANARAJA Mamay Mamay; Nurul Nurul; Titi Purwitasari; Raihan Nur Aulia
Ekalaya: Jurnal Pengabdian Kepada Masyarakat Indonesia Vol. 2 No. 1 (2023): Ekalaya Journal
Publisher : Nindikayla Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.231 KB) | DOI: 10.57254/eka.v2i1.45

Abstract

Stunting is still a major problem in Garut Regency, especially Wanaraja District, Sindang Mekar Village. Tackling stunting requires a multi-sectoral approach that includes increasing access to nutritious food and offering better feeding practices. The use of family medicinal plants (TOGA) is one of the prevention efforts to overcome stunting, one of which is the manufacture of food supplements. The purpose of this service is to increase community knowledge and understanding regarding the use of family medicinal plants (TOGA) in treating stunting in toddlers, in addition to increasing community skills in using family medicinal plants to improve skin health. The stages of implementing community service start from preparation for supervision, implementation to evaluation. The service provided counseling and demonstrations on the manufacture of food supplements made from family medicinal plants such as turmeric, temulawak and lemon grass. After the community service activities were carried out, an increase in the knowledge and skills of families of stunted children in the use of family medicinal plants (TOGA) was obtained
Magnesium and insulin leaf combination on glucose, low-density lipoprotein and malondialdehyde levels in wistar rats with diabetes mellitus Astari Nurisani; Mamay Mamay; Ana Hidayati Mukaromah; Purwanto AP
Science Midwifery Vol 11 No 1 (2023): April: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i1.1226

Abstract

Diabetes mellitus is a metabolic disease characterized by chronic hyperglycemia due to impaired insulin secretion, insulin receptor sensitivity and/or both. Hyperglycemia can trigger oxidative stress conditions that can increase the risk of micro and macrovascular complications. Preventive efforts need to be done such as giving supplements and traditional therapies that have low side effects, namely magnesium and insulin leaves. This research is experimental type with post test only control group. The samples in this study were 30 male wistar rats weighing 170-300g which had healthy conditions adapted for 7 days, given standard feed and given ad libitum drinking water. The results showed that the glucose level in the treatment group 3 when compared to the negative control group had a percentage decrease of 26.49%. LDL and MDA levels were lower in the combination treatment group of magnesium and insulin leaves when compared to the negative control group. The ANOVA results showed that there was a significant difference between the groups in the glucose test results (p < 0.05), and there was no significant difference between the groups in the LDL and MDA results (p > 0.05). The conclusion of this study was that the best results when compared to negative controls were the combination of magnesium at a dose of 300 mg and insulin leaves at a dose of 300 mg/KgBB had a decreased percentage of LDL levels by 52.38% and MDA levels of 29.11%, while for glucose levels the combination of magnesium doses 300 mg and insulin leaves 75 mg/KgBW had a percentage reduction of 26.49%
Isolation and identification of escherichia coli in well water located in Garut Regency Sugiah Sugiah; Gina Nafsa Mutmaina; Mamay Mamay; Astari Nurisani
Science Midwifery Vol 11 No 1 (2023): April: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i1.1236

Abstract

Water is one of the most important needs for daily life, especially well water. In the Garut district area well water is used for daily activities ranging from washing, drinking, bathing, ablution to being used for drinking water consumption. This study aims to isolate and identify well water from 10 villages located in the Garut Regency. Sampling was done using purposive sampling method. The test was followed by a presumptive test using Lactose Broth (LB), a reinforcing test using 2% BGLB media and a complementary test consisting of EMB agar media and biochemical tests. The results showed that of the 10 well water samples, 7 met the requirements of proper water quality and 3 did not meet the requirements of clean water with MPN values from three villages, namely Kampung Tanjung kamuning 200/ml, Kampung salaawi 241/100ml and Kampung Cipepe 241/100ml. EMB agar media is characterized by the formation of a metallic green color with black spots colonies and biochemical tests that show the well water has been contaminated with Escherichia coli bacteria.
Pemeriksaan C-Reactive Protein (CRP) Kualitatif dan Semi Kuantitatif pada Penderita Tuberkulosis Astari Nurisani; Mamay Mamay; Meti Rizki Utari; Zahara Farhan; Wisnu Arya Pratama
Jurnal Kesehatan Vol. 10 No. 2 (2022): Oktober: Health Journal "Love That Renews"
Publisher : Sekolah Tinggi Ilmu Kesehatan Santo Borromeus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55912/jks.v10i2.99

Abstract

Infeksi akibat bakteri tuberkulosis akan mengakibatkan peradangan didalam tubuh yang akan merangsang munculnya C-Reactive Protein sebagai biomarker peradangan. C-Reactive Protein (CRP) merupakan molekul polipeptida kelompok pentraxin yang diproduksi di hati dan dikontrol oleh sitokin khususnya Interleukin-6 (IL-6). CRP bermanfaat untuk memonitor perkembangan penyakit. Konsentrasi CRP berhubungan dengan tingkat keparahan penyakit,infeksi dan efek pengobatan. Tujuan penelitian ini adalah untuk melihat gambaran kadar CRP (C-Reactive Protein) pada penderita tuberkulosis di RSUD Dr. Slamet Garut. Pemeriksaan CRP dilakukan secara kualitatif dan semi kuantitatif. Hasil pemeriksaan CRP pada 30 sampel yang telah diperiksa didapat hasil positif sebanyak 24 orang (80%), dan negatif sebanyak 6 orang (20%). Berdasarkan hasil pemeriksaan diperoleh kadar CRP pada pasien tuberkulosis positif didapat hasil beragam antara 6 mg/L sampai 192 mg/L. Hasil penelitian ini menggambarkan adanya respon inflamsi CRP terhadap infeksi bakteri tuberkulosis. Saran dalam penelitian ini adalah data penelitian ini digunakan sebagai data acuan penelitian selanjutnya dalam pemeriksaan CRP metode kuantitatif mengenai gambaran kadar CRP sebelum dan sesudah pengobatan..
Co-Authors Acep Gunawan Aldi Renaldi Septian Ana Hidayati Mukaromah Astari Nurisani Diah Wardani Diah Wardani Elsa Zalfa Agustin Fathul Hakim Gina Nafsa Mutmaina Gina Nafsa Mutmainna Gina Nafsa Mutmainna Iin Patimah Meti Rizki Utari Muhammad Hadi Sulhan N Ai Erlinawati Nita Wahyuni Nurani Ai Erlinawati Nurul Nurul Purwanto AP Raihan Nur Aulia Ranti Nurwantika Riki Ananda Rina Herlina Sugiah Sugiah Tantri Puspita Tantri Puspita Titi Purwitasari Tri Rahmawati Wisnu Arya Pratama Yadi Apriyadi Zahara Farhan