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DETEKSI MOLEKULER BAKTERI Escherichia coli SEBAGAI PENYEBAB PENYAKIT DIARE DENGAN MENGGUNAKAN TEHNIK PCR Ismaun Ismaun; Muzuni; Nur Hikmah
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 6 No. 2 (2021): Bioma : Juli - Desember 2021
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v6i2.13194

Abstract

Penelitian ini dilakukan untuk mendeteksi secara molekuler bakteri Escherichia coli sebagai penyebab penyakit diare dengan menggunakan tehnik PCR. DNA genomik Escherichia coli diekstraksi menggunakan metode boiling, kemudian diamplifikasi menggunakan primer 16E1 dan 16E2. Hasil PCR positif Escherichia coli ditunjukkan dengan adanya pita fragmen DNA pada ukuran sekitar 584 pasang basa pada gel elektroforesis. Dalam penelitian ini sampel yang digunakan adalah sebanyak delapan titik dengan distribusi empat titik dari sampel air sumur bor dan empat dari air sumur galian. Dari delapan titik sampel, ada satu sampel yang terdeteksi secara molekuler yaitu mengandung Escherichia coli yaitu dengan kode sampel ASB 3yang dibuktikan dengan adanya pita DNA. Hasil penelitian menunjukkan bahwa metode PCR dapat mendeteksi Escherichia coli secara spesifik dan lebih cepat. Kata Kunci : Air, Bakteri Escherichia coli, Isolasi DNA, Tehnik PCR.
Pengaruh Pemanfaatan Laboratorium dan Nilai Karakter Terhadap Hasil Belajar IPA Siswa Kelas VIII Nur Hikmah; Kiki Nurzila
KULIDAWA Vol 3, No 1 (2022): Mei
Publisher : IAIN Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31332/kd.v3i1.3024

Abstract

This study aims to determine the effect of laboratory utilization and character values on science learning outcomes for SMPN 2 Bonegunu students. This research is a quantitative study with a research sample of all students in class VIII as many as 50 students. This study used 2 types of instruments, namely instruments to measure character values in the form of test questions and instruments to measure laboratory utilization in the form of questionnaires. The data analysis technique used is descriptive analysis technique. The results showed that: there was an effect of using the laboratory on student science learning outcomes, with a significant value of 0.000 <0.05, with a contribution of 96.7% and there was an effect of character values on student science learning outcomes with a significant value of 0.007 <0.05, with a contribution of 96.7%. It can be concluded that the utilization of the character value laboratory influences the learning outcomes of Class VIII students of SMPN 2 Bonegunu.Keywords: Laboratory Utilization, Character Value, Science’s Learning Outcomes
Meningkatkan Nilai Religius Pada Anak-Anak Melalui Kegiatan Keagamaan Di Desa Lawekara Aldi Hermawan Handoko; Miftahul Khaer; Afa Sri Bulan; Nur Hikmah; Imelda Wahyuni
INSANIYAH Vol 1, No 2 (2022): Desember 2022
Publisher : IAIN Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31332/insaniyah.v1i2.5809

Abstract

Menciptakan   suatu   sumber   daya   manusia   yang   unggul   dan   berkarakter merupakan salah satu hal yang perlu diperhatikan oleh pemerintah saat ini. Diberbagai literature dijelaskan bahwa karakter merupakan salah satu factor penting yang menentukan kualitas suatu Negara. Bergerak dari desa Lawekara penguatan pendidikan karakter akan di mulai melalui beberapa kegiatan keagamaan di masjid melalui pendidikan, pembentukan jati diri dan pengembangan potensi. Tujuan dari pengabdian ini adalah untuk membentuk karakter anak-anak sejak dini melalui penguatan niali-nilai religius seabagai upaya mempersiapkan generasi yang unggul. Pengabdian ini dilaksanakan oleh tujuh mahasiswa peserta KKN Reguler IAIN Kendari yang terdiri dari berbagai  macam  program studi,  diantaranya  program studi Pendidikan  Agama Islam, Pendidikan Guru Madrasah Ibtidaiyah, Tadris Bahasa Inggris, Tadris Biologi, Manejemen Pendidikan Islam, Ekonomi Syari’ah, dan Komunikasi Penyiaran Islam. Hasil pengabdian ini berupa mengajar BTQ, mengajar ilmu agama, yasinan rutin, khutbah jum’at, dan memakmurkan masjid melalui kegiatan sholat lima waktu secara berjama’ah. Kata Kunci: SDM Unggul, Nilai-Nilai Religius, KKN Desa Lawekara
The Development of an Ethnoscience-Based Science Module at Eucalyptus Oil Refining in Buru Regency to Reduce Student Misconceptions Muhammad Rizal Hardiansyah; Rahmiati Darwis; Nur Hikmah
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.6439

Abstract

This research is based on the limited learning resources that students can use independently. This research aims to develop an ethnoscience-based science module on the process of refining eucalyptus oil to determine the feasibility, practicality, and effectiveness of the module in reducing student misconceptions. The method used was research and development R&D. The research data obtained was analyzed using percentage descriptions. The results of the science module feasibility test showed that the feasibility of the material obtained a score of 88% with very feasible criteria. The feasibility of the media obtained a score of 89% with very feasible criteria. Furthermore, the feasibility of language obtained a score of 80% with appropriate criteria. The practicality of the module was tested through analysis of student responses to the module developed and a practicality score of 87% was obtained with very good criteria. This means that the ethnoscience-based science module developed has very good practicality in terms of a) attractiveness of the module, b) ease of use, c) ease of understanding the contents of the module, d) completeness of the contents of the module, and e) conformity with character values ethnoscience. According to the results of the analysis of learning outcomes tests accompanied by the Certainty of Response Index, it shows that the level of student misconceptions has decreased by 34% accompanied by an increase in the level of student understanding of concepts by 37%, and students who do not understand concepts have decreased by 3%. This shows that the module developed is effective in reducing student misconceptions