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All Journal Biota Jurnal Ilmiah Wahana Pendidikan
Priscinya Christiana Debora
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Kajian Mekanisme Molekuler Golongan Obat Antihipertensi Dalam Menghambat Angiotensin-Converting Enzyme (ACE) Jekmal Malau; Munir Alinu Mulki; Nur Komala Fitri; Najla Yusiana Wahyudi; Priscinya Christiana Debora; Mulidini Mulidini; Nolla Olipia Elva Megrian; Nissa Khalida Hanum
Jurnal Ilmiah Wahana Pendidikan Vol 9 No 2 (2023): Jurnal Ilmiah Wahana Pendidikan
Publisher : Peneliti.net

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.478 KB) | DOI: 10.5281/zenodo.7571470

Abstract

Hypertension or increased blood pressure is a chronic disease that requires long treatment, even for life. Patients with hypertension must control their blood pressure to avoid complications. In the body, there are components of the renin-angiotensin system, namely the angiotensin-converting enzyme (ACE) which can indirectly increase blood pressure, so the treatment is to inhibit ACE. In this article, the method used is by literature study of several accredited journals that has published from 2012 to 2022. ACE inhibitor class drugs are often used for the treatment of hypertension because they have a mechanism of inhibiting the conversion enzyme peptidyl dipeptidase, so that angiotensin I is not hydrolyzed to angiotensin II and can be contributed for dilating blood vessels as well so that it effectively lowers blood.
Comparison of Two PCR Primer Sets for In-House Validation of GHSR Gene Variation Detection Employing Artificial Recombinant Plasmid Approach Ahsanal Kasasiah; Jekmal Malau; Sekar Andjung Tresnawati; Priscinya Christiana Debora; Nur Komala Fitri; Saarah Hamidah Asmara Indratno; Asman Hitopik; Eriyanti Astika; Anisa Aula Rahma; Fikri, Al Mukhlas
Biota Vol 10 No 2 (2024): Jurnal Biota 2024
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v10i2.21166

Abstract

Stunting is a significant global public health problem caused by long-term dietary deficits that affect many children worldwide. Both environmental and genetic factors, including variants in the GHSR gene, play a crucial role in stunted growth. This study used an artificial recombinant plasmid DNA method to evaluate two primer set combinations for identifying DNA variants in the GHSR gene. Selecting suitable primer sets for identifying GHSR genetic variants linked to stunting is essential, as evidenced by PCR and sequencing techniques. The target gene, based on the GHSR reference sequence, consists of eight DNA variations (ΔQ36, G57G, P108L, L118L, R159R, C173R, D246A, and A277P). A recombinant plasmid was created by inserting a 1000 bp fragment of the GHSR gene into the pUC57 backbone. Primer sets were chosen based on their capacity to amplify these eight genetic variations and were optimized and validated using PCR methods. PCR and bi-directional sequencing verified the existence of surrounding DNA and specific single nucleotide variants (SNVs). In our study, we discovered four changes in the DNA sequence (R159R G>A, C173R T>C, D246A A>C, and A277P G>C) using the E1_F2/E1_R3 primer pair. Additionally, a new combination of primers (E1_F1/E1_R3) effectively detected seven DNA sequence mutations (ΔQ36 del CAG, G57G C>T, P108L C>T, L118L C>T, R159R G>A, C173R T>C, and D246A A>C). We have developed a new combination of forward and reverse primers to identify seven SNVs in the GHSR gene, which could serve as a diagnostic tool in clinical laboratory settings.
Co-Authors Ahsanal Kasasiah Anisa Aula Rahma Asman Hitopik Eriyanti Astika Fikri, Al Mukhlas Jekmal Malau Mulidini Mulidini Munir Alinu Mulki Najla Yusiana Wahyudi Nissa Khalida Hanum Nolla Olipia Elva Megrian Nur Komala Fitri Saarah Hamidah Asmara Indratno Sekar Andjung Tresnawati