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All Journal Biota Jurnal Ilmiah Wahana Pendidikan
Asman Hitopik
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Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Review Jurnal : Faktor Pengaruh Hubungan Gagal Ginjal Akut Dan Covid-19 Mally Ghinan Sholih; Munir Alinu Mulki; Asman Hitopik; Ibtisamah Haura; Madame Marselly Sebayang; Nurfirah Mubarok; Rachel Noveriachristie Balapadang; Rima Putri Ramadhani; Rifa Nur Alia
Jurnal Ilmiah Wahana Pendidikan Vol 9 No 16 (2023): Jurnal Ilmiah Wahana Pendidikan
Publisher : Peneliti.net

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.8248782

Abstract

COVID-19 is a disease caused by infection with the SARS-CoV-2 virus and has become a global pandemic outbreak because it is highly contagious and causes severe infections of the respiratory system. The SARS-CoV-2 virus causes infection primarily in the lungs and upper respiratory tract but can also infect other organs such as the brain, kidneys, intestines and liver. During the pandemic, many serious complications from COVID-19 were reported and one of them was acute kidney failure. Acute kidney failure is a condition where kidney dysfunction occurs quickly and suddenly. This study aims to investigate the factors that influence the relationship between acute kidney failure and COVID-19 infection. This research method begins with a literature search through the PubMed Identifier (PMID) database within a predetermined range of years and using the keywords pathophysiology, COVID-19, and acute kidney failure. The results of the review show that there are several factors that play a role in influencing the relationship between acute kidney failure and COVID-19 infection. Kidney damage in COVID-19 patients can occur due to a direct attack from the SARS-CoV-2 virus, the occurrence of a cytokine storm, or an indirect attack which can be caused by nephrotoxin exposure due to medical care given to the patient, secondary infection, or other factors. such as elderly and chronically ill patients. Based on the findings, it is known that there are risk factors, pathophysiological mechanisms, and patient medical care that can influence the relationship between acute kidney failure and COVID-19 infection. From the results of this analysis, it is hoped that the findings obtained can serve as a basis and guideline in the management of acute kidney failure as a complication of COVID-19.
Comparison of Two PCR Primer Sets for In-House Validation of GHSR Gene Variation Detection Employing Artificial Recombinant Plasmid Approach Ahsanal Kasasiah; Jekmal Malau; Sekar Andjung Tresnawati; Priscinya Christiana Debora; Nur Komala Fitri; Saarah Hamidah Asmara Indratno; Asman Hitopik; Eriyanti Astika; Anisa Aula Rahma; Fikri, Al Mukhlas
Biota Vol 10 No 2 (2024): Jurnal Biota 2024
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v10i2.21166

Abstract

Stunting is a significant global public health problem caused by long-term dietary deficits that affect many children worldwide. Both environmental and genetic factors, including variants in the GHSR gene, play a crucial role in stunted growth. This study used an artificial recombinant plasmid DNA method to evaluate two primer set combinations for identifying DNA variants in the GHSR gene. Selecting suitable primer sets for identifying GHSR genetic variants linked to stunting is essential, as evidenced by PCR and sequencing techniques. The target gene, based on the GHSR reference sequence, consists of eight DNA variations (ΔQ36, G57G, P108L, L118L, R159R, C173R, D246A, and A277P). A recombinant plasmid was created by inserting a 1000 bp fragment of the GHSR gene into the pUC57 backbone. Primer sets were chosen based on their capacity to amplify these eight genetic variations and were optimized and validated using PCR methods. PCR and bi-directional sequencing verified the existence of surrounding DNA and specific single nucleotide variants (SNVs). In our study, we discovered four changes in the DNA sequence (R159R G>A, C173R T>C, D246A A>C, and A277P G>C) using the E1_F2/E1_R3 primer pair. Additionally, a new combination of primers (E1_F1/E1_R3) effectively detected seven DNA sequence mutations (ΔQ36 del CAG, G57G C>T, P108L C>T, L118L C>T, R159R G>A, C173R T>C, and D246A A>C). We have developed a new combination of forward and reverse primers to identify seven SNVs in the GHSR gene, which could serve as a diagnostic tool in clinical laboratory settings.
Co-Authors Ahsanal Kasasiah Anisa Aula Rahma Eriyanti Astika Fikri, Al Mukhlas Ibtisamah Haura Jekmal Malau Madame Marselly Sebayang Mally Ghinan Sholih Munir Alinu Mulki Nur Komala Fitri Nurfirah Mubarok Priscinya Christiana Debora Rachel Noveriachristie Balapadang Rifa Nur Alia Rima Putri Ramadhani Saarah Hamidah Asmara Indratno Sekar Andjung Tresnawati