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Tri Saptari Haryani
Pakuan University
Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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Journal : Journal of Science Innovare

 1 
Isolation And Identification Of Pathogen Mushroom Types On Umbi Talas (Colocasia Esculenta (L.) Schoot) Post Harvest Sulistiyono, Fitria Dewi; Haryani, Tri Saptari
Journal of Science Innovare Vol 1, No 01 (2018): Journal of Science Innovare
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.273 KB) | DOI: 10.33751/jsi.v1i01.685

Abstract

The taro tuber (Colocasia esculenta) is an important source of carbohydrates for an important energy producer whose portion has a fairly bright and profitable development prospect. Taro is not only used as a food source, it can be used for industrial purposes, for example raw materials of cosmetics and plastic. Taro is very easy to recognize and varied with other preparations because it has a distinctive flavor. Increased production is the only major consideration in taro cultivation. Efforts to increase production are influenced by limiting factors that are often experienced in the community. Such barrier is a pathogen attack both on the ground and post harvest. The purpose of this study is to isolate and identify the types of pathogenic fungi found in taro tubers. The method used is microscopic and macroscopic identifications. The results of identification with the macroscopic and microscopic observations obtained by the genus Aspergillus, Candida, Sclerotium, Fusarium, Mucor, and Rhizopus. Keywords: Taro, Identification, Pathogenic Fungi
Isolation And Identification Of Pathogen Mushroom Types On Umbi Talas (Colocasia Esculenta (L.) Schoot) Post Harvest Fitria Dewi Sulistiyono; Tri Saptari Haryani
Journal of Science Innovare Vol 1, No 01 (2018): Journal of Science Innovare
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.273 KB) | DOI: 10.33751/jsi.v1i01.685

Abstract

The taro tuber (Colocasia esculenta) is an important source of carbohydrates for an important energy producer whose portion has a fairly bright and profitable development prospect. Taro is not only used as a food source, it can be used for industrial purposes, for example raw materials of cosmetics and plastic. Taro is very easy to recognize and varied with other preparations because it has a distinctive flavor. Increased production is the only major consideration in taro cultivation. Efforts to increase production are influenced by limiting factors that are often experienced in the community. Such barrier is a pathogen attack both on the ground and post harvest. The purpose of this study is to isolate and identify the types of pathogenic fungi found in taro tubers. The method used is microscopic and macroscopic identifications. The results of identification with the macroscopic and microscopic observations obtained by the genus Aspergillus, Candida, Sclerotium, Fusarium, Mucor, and Rhizopus. Keywords: Taro, Identification, Pathogenic Fungi
Isolation And Identification Of Pathogen Mushroom Types On Umbi Talas (Colocasia Esculenta (L.) Schoot) Post Harvest Fitria Dewi Sulistiyono; Tri Saptari Haryani
Journal of Science Innovare Vol 1, No 1 (2018): Journal of Science Innovare, Volume 01 Number 01 2018
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v1i01.685

Abstract

The taro tuber (Colocasia esculenta) is an important source of carbohydrates for an important energy producer whose portion has a fairly bright and profitable development prospect. Taro is not only used as a food source, it can be used for industrial purposes, for example raw materials of cosmetics and plastic. Taro is very easy to recognize and varied with other preparations because it has a distinctive flavor. Increased production is the only major consideration in taro cultivation. Efforts to increase production are influenced by limiting factors that are often experienced in the community. Such barrier is a pathogen attack both on the ground and post harvest. The purpose of this study is to isolate and identify the types of pathogenic fungi found in taro tubers. The method used is microscopic and macroscopic identifications. The results of identification with the macroscopic and microscopic observations obtained by the genus Aspergillus, Candida, Sclerotium, Fusarium, Mucor, and Rhizopus. Keywords: Taro, Identification, Pathogenic Fungi
Morphological and Molecular Characterization of Pestalotiopsis sp. Associated with Leaf Spot Disease in Eucalyptus pellita Endrawati, Silvia; Haryani, Tri Saptari; Lelana, Neo Endra
Journal of Science Innovare Vol 8, No 1 (2025): Journal of Science Innovare, Volume 08 Number 02 2025
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v8i1.12814

Abstract

Leaf spot disease is one of the most common foliar infections affecting Eucalyptus pellita, a fast-growing tree species widely cultivated for pulp and paper industries in Indonesia. This study aimed to isolate and identify the fungal pathogen associated with the disease through morphological and molecular characterization. Symptomatic leaves were collected from E. pellita stands in KHDTK Parungpanjang, and fungal isolation was performed on Potato Dextrose Agar (PDA). Morphological observations revealed that the isolated fungus exhibited white colonies with purple pigmentation at the center and conidia containing four septa—typical characteristics of Pestalotiopsis species. Molecular identification was conducted using Internal Transcribed Spacer (ITS) region sequencing of rDNA, followed by BLAST analysis against the NCBI GenBank database. Sequence alignment and phylogenetic tree reconstruction confirmed that the isolate (PT.E002) shared 100% similarity with Pestalotiopsis mangiferae, P. clavispora, P. maculans, and P. microspora. The phylogenetic analysis indicated a close relationship between the PT.E002 isolate and P. maculans. These results validate the presence of Pestalotiopsis sp. as the main pathogen associated with leaf spot disease in Eucalyptus pellita. The integration of morphological and molecular analyses enhances the accuracy of pathogen identification, contributing valuable insights for forest disease management and sustainable Eucalyptus cultivation programs.
Co-Authors Ade Kusmiati Ardiani, Weni Arti Wahyu Utami Azzahro, Fiona Bina Lohita Sari Cecep Sudrajat DARMAWAN PANGERAN Dede Giwang Maelani Dina Agustina Djarot, Prasetyorini Eman eman Endrawati, Silvia Fauziah, Salsabela Sipa Fiona Azzahro Firman Rezaldi Fitria Dewi Sulistiyono Fitria Eka Resti Wijayanti Ghina Athyah Wahid Hariningsih, Yetti Indah Nur Sayyidah Intan Lestari Ismanto Ismanto Ismanto Jihan Nabilla Khaerani, Nuraliefah Kurniawati, Ulan MESAK TOMBE Moerfiah Mutiara Siti Nurfajriah Neo Endra Lelana, Neo Endra Oom Komala Reni Purbanova Rifkarosita Putri Ginaris SATA YOSHIDA SRIE RAHAYU Sinta Wisma Sari Siti Suryani Tahier Sri Wiedarti Sudrajat, Cecep Sudrajat, Cecep Sukardi Sugeng Rahmad Sukardi Sugeng Rahmad Syafrudin, Yudi Tampubolon, Jureka Tombe, Olivia Mersylia Tri Yudianto Triastinurmiatiningsih Triastinurmiatiningsih, Triastinurmiatiningsih Usman Setiawan Uswatun Hasanah Wahyu Prihatini Yulianti Bramasto Yulianti Bramasto Zulkhoir Zulkhoir