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All Journal The Journal of Experimental Life Sciences (JELS) Journal of Innovation and Applied Technology Research Journal of Life Science Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Pengabdian Magister Pendidikan IPA Journal of Aquaculture and Fish Health Journal Of Aquaculture Development and Environment
Agoes Soeprijanto, Agoes
Brawijaya University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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The Effect of Difference Temperature on Cortisol, Glucose and Glycogen level of Uceng Fish (Nemacheilus fasciatus) Harun Wijaya; Maheno Sri Widodo; Agoes Soeprijanto
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.651 KB) | DOI: 10.21776/ub.jels.2019.009.01.01

Abstract

Nemacheilus is one of the genera of freshwater fish. The existence of Uceng fish (Nemacheilus fasciatus) in public waters is increasingly rare. Changes in water temperature in the maintenance medium will affect the physiological processes of fish. The purpose of this study was to analyze physiological responses (blood glucose levels, cortisol, and glycogen hormones) of Uceng Fish (N. fasciatus) which were incubated at different temperatures and find out the initial time of Uceng fish adaptation to its new environment. Total of 200 fish were collected from wild catches in the Lekso River, Blitar. Fish tissue collection was carried out to test the profile of glucose, cortisol, and glycogen. The research activities at this stage were carried out by raising the Uceng fish from nature to a cultivation container in an aquarium of size 50 x 30 x 30 cm that given the different temperatures (20°C, 24°C, 28°C and 32°C), and each aquarium is filled with 10 Uceng fish and will be kept for 14 days. ELISA method was used to quantify the parameter. The result of this study showed highest peak of cortisol levels and blood glucose levels was achieved in the treatment of 24°C on the 10th day and the lowest in the treatment temperature of 28°C on the 6th day. The highest glycogen peak was reached at 32°C on day 10 and the lowest peak was reached at 28°C on day 2. We conclude that temperature affects the physiological response (cortisol, blood glucose, and glycogen) of Uceng fish (N. fasciatus). High levels of cortisol and blood glucose indicate stressful fish. Keywords: Fish, Nemacheilus fasciatus, Physiological, Temperature, Uceng.
Influence of Different Pulse Length towards Motility and Viability of Ornamental Japanese Carp (Cyprinus carpio Var. Koi) Sperm through Electroporation Method Diana Aisyah; Agoes Soeprijanto; Yuni Kilawati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2016.007.01.06

Abstract

Successful studies about implementation of electroporation method which sperm becomes gene transfer medium for fish transgenesis have reported. Motility and viability are two major factors in successful of electroporation. The objective of the study was to determine optimal pulse length of electroporation towards the motility and viability of Koi fish sperm. The study was conducted at the Central Laboratory of Biological Sciences and Reproduction of Brawijaya University. The data analysis was conducted using completely randomized design. The electroporation method was carried out using voltage of 30 volt with pulse number of 4 times. The pulse length of the electroporation (based on treatment) was 0.5, 1 and 1.5 ms. The result showed the highest percentage of motility was 3.67% in treatment A (pulse length 0.5 ms) with coefficient correlation R2 = 0.9643 and the best percentage of viability was 79.67% in treatment A (pulse length 0.5 ms) with coefficient correlation R2 = 1. Correlation between the pulse length treatment (electroporation) toward motility and viability of Koi fish sperm, in which longer duration of pulse length treatment (electroporation) would decrease the motility and viability percentage of the fish sperm.Keywords: Electroporation, Motility, Pulse length, Sperm, Viability.
Peningkatan Produktivitas Budidaya Ikan Nila (Oreochromis Niloticus) Melalui Transfer Biofloc Technology (BFT) untuk Mendukung Penguatan Kemandirian Desa Mojorejo, Kecamatan Junrejo, Kota Batu Soeprijanto, Agoes; Jamroni, Mofit; Aisyah, Diana; Supriyadi, Supriyadi; Subandi, Hendi
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 4 (2023): Oktober-Desember 2023
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Aquaculture business, especially tilapia cultivation in Mojorejo village, did not run as expected due to low fish production and limited community knowledge regarding cultivation techniques. Some of the problems faced by the cultivator group are that the fishery business has not been well programmed because there is no socialization of business potential in the fishery sector, effective tilapia cultivation techniques are not yet known, and assistance is needed related to aquaculture management to be able to optimize tilapia aquaculture production. So that the main goal in this community service activity is to increase the productivity of tilapia (Oreochromis niloticus) cultivation through transfer of biofloc technology (BFT) to support strengthening the independence of Mojorejo village, Junrejo District, Batu city. The methods used include surveys and site reviews, BFT socialization and training, BFT transfers, and periodic assistance. The results obtained in this activity are that partners understand the basic principles of tilapia aquaculture with the BFT system, partners understand input and output settings in tilapia cultivation with the BFT system, partners can culture biofloc bacteria, partners can know the C:N ratio in aquaculture ponds and partners can cultivate with the BFT system so that added value to production is increasing.
Co-Authors Abdul Wafi Aisyah, Diana Diana Aisyah Dimas Adetia Rikianto Emir Ma’arif Imanudin Harun Wijaya Heri Ariadi I Nengah Gde Sugestya Immanuel A.H. Pandaingan Jamroni, Mofit Jojo Subagja Kurniawan Kurniawan Maheno Sri Widodo, Maheno Sri Muhammad Fakhri Otong Zenal Arifin Pratama Deffi Samuel R Adharyan Islamy Saidah Luthfiyah Sholikah, Lik Anatus Subandi, Hendi Supriyadi Supriyadi Toni Yulian Kusmardani Yuni Kilawati Yunita Maemunah Yunita Maemunah Yunita Maimunah