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All Journal Reaktor Agro Bali: Agricultural Journal Jurnal Teknik Kimia Indonesia Widya Teknik

Ery Susiany Retnoningtyas

Chemical Engineering Department, Widya Mandala Catholic University Surabaya

Author-ID : 5922580

Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Energy Materials Science & Nanotechnology

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BIOSINTESA SENYAWA FENOLIK ANTIOKSIDAN DARI LIMBAH KULIT PISANGKEPOK (Musa acuminata balbisiana C.) SECARA FERMENTASI SUBMERGED MENGGUNAKAN RHIZOPUS ORYZAE Andre Siswaja; Adhitia Gunarto; Ery Susiany Retnoningtyas; Aning Ayucitra
Reaktor Volume 15 No.4 Oktober 2015
Publisher : Dept. of Chemical Engineering, Diponegoro University

BIOSYNTHESIS OF PHENOLIC ANTIOXIDANT COMPOUNDS FROM KEPOK BANANA PEEL WASTE (Musa acuminata balbisiana C.) USING SUBMERGED FERMENTATION BY RHIZOPUS ORYZAE. Phenolic antioxidant compounds can be formed through a process of biosynthesis with the help of microorganism. Kepok banana peel waste contains nutrients that support the growth of Rhizopus oryzae producing phenolic antioxidant compounds through its secondary metabolism. The objective of this research was to study the effects of fermentation time, concentration of Kepok banana peel extracts, and concentration of (NH4)2SO4 on Total Phenolic Content (TPC) of extracts substrate. Total Antioxidant Capacity (TAC) of extracts with the highest TPC value was also measured. TPC of extracts were analyzed by Folin-Ciocalteu method whilst TAC by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Rhizopus oryzae was grown on a substrate containing kepok banana peel extract (500 g of kepok banana peel/L of water and 1000 g of kepok banana peel/L of water), (NH4)2SO4, and other nutrients. Results showed that extracts with the highest phenolic content were obtained after 72 hours fermentation on substrate containing 32.69 mg/mL of glucose (concentration of kepok banana peel 1000 g/L of water) and 0.25% w/v (NH4)2SO4. The substrate had TPC of 582.07 mg Gallic Acid Equivalent (GAE)/L extract with TAC of 88.37%. Keywords: biosynthesis; kepok banana peel; phenolic antioxidant; Rhizopus oryzae Abstrak Senyawa fenolik antioksidan dapat terbentuk melalui proses biosintesa dengan bantuan Rhizopus oryzae menggunakan substrat limbah kulit pisang kepok. Limbah kulit pisang kepok belum banyak dimanfaatkan dan memiliki nilai ekonomi rendah. Di sisi lain, kulit pisang kepok mengandung sejumlah nutrisi yang dapat mendukung pertumbuhan Rhizopus oryzae dalam memproduksi senyawa fenolik antioksidan. Penelitian ini bertujuan untuk mempelajari pengaruh waktu fermentasi, konsentrasi ekstrak kulit pisang kepok, dan konsentrasi (NH4)2SO4 terhadap perolehan senyawa fenolik, serta mempelajari Total Antioxidant Capacity (TAC) untuk ekstrak dengan perolehan senyawa fenolik tertinggi. Dalam penelitian ini, Rhizopus oryzae ditumbuhkan pada substrat ekstrak kulit pisang kepok dengan variasi 500 g kulit pisang kepok/L air dan 1000 g kulit pisang kepok/L air serta penambahan (NH4)2SO4 dengan variasi konsentrasi. Total Phenolic Content (TPC) diukur menggunakan metode Folin-Ciocalteu, sedangkan Total Antioxidant Capacity (TAC) dianalisis dengan metode DPPH (2,2-diphenyl-1-picrylhydrazyl). Hasil penelitian menunjukkan bahwa TPC tertinggi terkandung dalam konsentrasi ekstrak kulit pisang kepok 1000 g/L air dengan penambahan 0,25% b/v (NH4)2SO4) yaitu 582,07 mg Gallic Acid Equivalent (GAE)/L ekstrak dengan TAC 88,37% setelah fermentasi 72 jam. Kata kunci: biosintesa; kulit pisang kepok; fenolik antioksidan; Rhizopus oryzae

APLIKASI CRUDE ENZIM SELULASE DARI TONGKOL JAGUNG (Zea mays L) PADA PRODUKSI ETANOL DENGAN METODE SIMULTANEOUS SACCHARIFICATION AND FERMENTATION (SSF) Ery Susiany Retnoningtyas; Antaresti Antaresti; Aylianawati Aylianawati
Reaktor Volume 14, No. 4, OKTOBER 2013
Publisher : Dept. of Chemical Engineering, Diponegoro University

Tongkol jagung yang melimpah sebagai produk samping dari hasil pengolahan industri jagung pipilan, memberikan peluang untuk dimanfaatkan sebagai substrat dalam produksi crude enzim selulase. Crude enzim selulase dari tongkol jagung selanjutnya diaplikasikan pada produksi etanol dengan memanfaatkan tongkol jagung juga dengan metode simultaneous saccharification and fermentation (SSF). Penelitian ini bertujuan untuk mempelajari pengaruh volume crude enzim selulase terhadap kadar etanol, kinerja crude enzim selulase dalam memproduksi etanol dan membandingkan kinerjanya dengan crude enzim komersial. Penelitian ini terbagi dalam dua tahap yaitu : pretreatment tongkol jagung, dan fermentasi. Tongkol jagung yang sudah dikeringkan, dihaluskan hingga menjadi serbuk dengan ukuran 12 mesh, dan diberi perlakuan hidrolisis dengan uap panas (steam explosion) selama 60 menit. Serbuk tongkol jagung difermentasi,dengan penambahan nutrisi, crude enzim selulase dan jamur Saccharomyces cerevisiae. Proses fermentasi dilakukan pada suhu 30oC selama 120 jam, sedangkan pengamatan dilakukan setiap 24 jam. Hasil penelitian menunjukkan produksi etanol yang dihasilkan dengan menggunakan crude enzim selulase dan crude enzim komersial berturut-turut adalah 1,28 dan 2,89 %.

Antimicrobial Activity of Kaffir Lime Peel Extract against Streptococcus mutans Rosalie Purwanto; Jeni Pabontong; Ery Susiany Retnoningtyas; Wenny Irawaty
Reaktor Volume 18 No. 4 December 2018
Publisher : Dept. of Chemical Engineering, Diponegoro University

Kaffir lime peels contain polyphenols as natural antioxidant and antimicrobial agent. The aims of this study were to (1) extract phenolics compounds from kaffir lime peels using water, ethanol 70% and ethanol 96% as the solvent, and (2) assess the antibacterial activity of the extract against Streptococcus mutans which is the main cause of dental caries. Research methodology includes preparation and extraction of polyphenols from kaffir lime peels, preparation of mouthwash based-kaffir lime peels extracts and evaluation the mouthwash ability to inhibit the growth of Streptococcus mutans. The results show water exhibited the best solvent to extract polyphenols among the three solvents. The total phenolics content in the water extract was observed at 11.42±0.48 mg GAE/g, whilst in the two ethanolic extracts were 10.91±0.87 and 8.87±0.53 mg GAE/g for ethanol 70 and 96%, respectively. Consequently, the water-based extract performed the highest antimicrobial activity. The highest inhibition zone was demonstrated by 100% extract of concentration extract variation. Although the inhibition zone of the mouthwash was smaller than the commercial product, the extract has the potential to be developed as a safe mouthwash for long-term usage.Keywords: dental caries; kaffir lime; mouthwash; peel; phenolic; Streptococcus mutans

Co-Authors . Antaresti . Susyana Adhitia Gunarto Adriana Anteng Anggorowati Andre Siswaja Aning Ayu Citra Aning Ayucitra Aning Ayucitra Anita Puspitasari Anita Yuliviana Antaresti Antaresti Aylianawati Aylianawati Aylianawati Aylianawati Ayuningtyas, Debora Berlian Adiguna Darwin Junaidi Delftria Rasubala Fandy Maramis Frengky W. Pribadi Herman Setiawan Jeni Pabontong Juanda, Kevina Tomiko Mario Christofer Kresna Pratama Santoso Nelsi K. Tanti Ong Wei Yong Rosalie Purwanto Sandy Budi Hartono Santoso, Shella Permatasari Victor Gala Welly Surya Naga Wenny Irawaty Yessica Arini Paskawati Yosephine Allita

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