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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Proceeding International Conference on Global Resource Conservation
Ika Agus Rini, Ika Agus
Institut Teknologi Sumatera
Agriculture, Biological Sciences & Forestry Environmental Science Health Professions Other

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Utilising Synthetic Biology to Produce Renewable Energy from Indonesia’s Biodiversity: Inspiration from iGEM Competition Kemal, Rahmat Azhari; Rini, Ika Agus; Oktaviani, Indah
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.209 KB)

Abstract

The emerging field of synthetic biology focuses on engineering biomolecular systems and cellular capabilities. It can be applied to a wide variety of applications, one of them is renewable energy production. Teams competing in the International Genetically Engineered Machine (iGEM) competition design and assemble biological devices able to do certain purposes such as producing renewable energy. Their ideas can be adapted by using compatible organisms available from Indonesia’s rich biodiversity. Several ideas are highlighted in this review. One of them is the idea of recycling methanol in glycogen that can be used as carbon source for biofuel production. Methanol dehydrogenase from Indonesia’s methylotrophic bacteria can substitute Bacillus methanolycus used in the system. The adapted ideas can serve as starting points for research, production, and implementation of renewable energy in Indonesia.Keywords: Biodiversity; iGEM; Indonesia; renewable energy; synthetic biology
DNA Barcoding for Selected Mangrove-Based Estuary Fishes from Way Kambas National Park, Lampung Province, Indonesia Ariyanti, Yanti; Rini, Ika Agus; Oktaviani, Indah; Leksikowati, Sovia Santi
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.04

Abstract

Over the past decade, DNA barcoding has provided new insight into fish ecology and biosystematics and led to new species' discovery. DNA barcoding is a method for the recognition and identification of species using short, standardised DNA fragments. The correct taxonomic identification of species is critical for the assessment and monitoring of biodiversity. This study applied DNA barcoding techniques to identify selected fish species from a mangrove-based estuary in Way Kambas National Park, Lampung Province, Indonesia. The gene encoding cytochrome c oxidase subunit I (COI) was amplified and bi-directionally sequenced from 22 specimens. The resulting 680 base pairs (bp) sequence was used to identify species, obtain phylogenetic information, and analyse genetic distances. A neighbour-joining tree was constructed based on the mitochondrial COI gene using the Kimura two-parameter model. This study also exhibits conservation status for those identified species. Our findings will facilitate future studies of fish species diversity in mangrove estuary-based ecosystems and provide preliminary data in policymaking in conservation areas such as National Park.
Co-Authors Leksikowati, Sovia Santi Oktaviani, Indah Rahmat Azhari Kemal Veithzal Rivai Zainal Yanti Ariyanti, Yanti