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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL TEKNOLOGI LINGKUNGAN BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Jurnal Sumberdaya Hayati Elkawnie Journal of Science and Applicative Technology Biologica Samudra Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat
Leksikowati, Sovia Santi
Department Of Biology, Institut Teknologi Sumatera
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Nursing Veterinary

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Isolasi Bakteri Indigineous dari Limbah Cair Tahu dalam Mendegradasi Protein dan Melarutkan Fosfat Asril, Muhammad; Oktaviani, Indah; Leksikowati, Santi
Jurnal Teknologi Lingkungan Vol. 20 No. 1 (2019)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.754 KB) | DOI: 10.29122/jtl.v20i1.3132

Abstract

ABSTRACTThe tofu industry in Indonesia is growing rapidly along with the consumption needs. This amount affects the production of the tofu wastewater that will be discharged into the environment. The waste contains high levels of BOD, COD, and high protein organic substances. The discharged of the tofu wastewater without going through a good process of separation and treatment caused odor and pollution in the water and the soil environment. This study aims to examine the amount of production, a chemical characteristic, and bacterial potential (the genus of Pseudomonas and Aeromonas, proteolytic bacterial isolates, and potential phosphate solvent bacteria) which can be used as a reference for bioconversion of higher tofu wastewater. The method used in this study was water sampling, chemical characteristic testing (BOD, COD, total protein, and pH), the bacterial isolation of Pseudomonas and Aeromonas using GSP media, isolation of proteolytic bacteria using nutrient agar + Skim milk media and testing the solubilizing phosphate ability in pikovskaya media. The results showed the production of the tofu liquid waste reached 4000 liters/day with BOD, COD, total protein and pH content is 3210 mg/ L, 7102 mg/L, 20.74% and 5, respectively. The amount of indigenous bacteria in wastewater from Pseudomonas were 72 x 102 cfu/ml), Aeromonas were 18 x 102 cfu/ml), and total proteolytic bacteria were 23.7 x 105 cfu/ml). Proteolytic bacteria obtained in this research were 28 isolates with diverse proteolytic abilities. In addition, 17 of the 28 proteolytic bacteria isolates have the ability to soluble phosphate on pikovskaya medium. These results show that the production of the tofu liquid waste is quite high. Therefore, the chemical character and the presence of bacteria in it have the potential to be developed and can be used as a reference for bioconversion of waste before being discharged into the environment.Keywords: Aeromonas, proteolytic bacteria, tofu wastewater, phosphate solubilizing bacteria, Pseudomonas ABSTRAKIndustri tahu di Indonesia berkembang sangat pesat seiring dengan kebutuhan konsumsi masyarakat Indonesia. Jumlah ini berpengaruh terhadap produksi limbah cair tahu yang akan dibuang ke lingkungan. Limbah tersebut memiliki kandungan BOD, COD dan bahan organik berupa protein yang cukup tinggi. Limbah cair tahu jika dibuang lingkungan tanpa melalui proses pemisahan dan perlakuan yang baik dapat menimbulkan bau dan pencemaran di lingkungan air dan tanah. Penelitian ini bertujuan untuk mengkaji jumlah produksi, karakteristik kimia dan potensi biologi (bakteri genus Pseudomonas dan Aeromonas, isolat bakteri proteolitik dan bakteri pelarut fosfat potensial) yang nantinya dapat digunakan sebagai acuan biokonversi limbah cair tahu yang semakin tinggi. Metode yang digunakan pada penelitian ini adalah pengambilan sampel, pengujian karakteristik kimia (BOD, COD, total protein dan pH), isolasi bakteri genus Pseudomonas dan Aeromonas pada media GSP, isolasi bakteri proteolitik di media Nutrien agar + Skim milk dan pengujian kemampuan melarutkan fosfat pada media pikovskaya. Hasil menunjukkan bahwa limbah cair yang diproduksi setiap harinya mencapai 4000 liter/hari dengan kandungan BOD, COD, total protein dan pH berturut-turut sebesar 3210 mg/L, 7102 mg/L, 20.74 % dan 5. Jumlah bakteri indigeous dalam limbah cair tahu dari genus Pseudomonas adalah 72 x 102 cfu/ml, genus Aeromonas adalah 18 x 102 cfu/ml dan total bakteri proteolitik adalah 23.7 x 105 cfu/ml. Bakteri proteolitik yang diperoleh berjumlah 28 isolat dengan kemampuan proteolitik yang cukup beragam. Selain itu, 17 dari 28 isolat bakteri proteolitik tersebut memiliki kemampuan melarutkan fosfat pada media pikovskaya. Hasil ini menunjukkan bahwa produksi limbah cair tahu yang cukup tinggi, karakteristik kimia serta keberadaan bakteri didalamnya memiliki potensi untuk dikembangkan dan dapat dijadikan sebagai acuan untuk biokonversi limbah sebelum dibuang ke lingkungan.Kata kunci: Aeromonas, bakteri proteolitik, limbah cair tahu, bakteri pelarut fosfat, Pseudomonas
DNA Barcoding for Selected Mangrove-Based Estuary Fishes from Way Kambas National Park, Lampung Province, Indonesia Ariyanti, Yanti; Rini, Ika Agus; Oktaviani, Indah; Leksikowati, Sovia Santi
Journal of Tropical Life Science Vol 11, No 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.04

Abstract

Over the past decade, DNA barcoding has provided new insight into fish ecology and biosystematics and led to new species' discovery. DNA barcoding is a method for the recognition and identification of species using short, standardised DNA fragments. The correct taxonomic identification of species is critical for the assessment and monitoring of biodiversity. This study applied DNA barcoding techniques to identify selected fish species from a mangrove-based estuary in Way Kambas National Park, Lampung Province, Indonesia. The gene encoding cytochrome c oxidase subunit I (COI) was amplified and bi-directionally sequenced from 22 specimens. The resulting 680 base pairs (bp) sequence was used to identify species, obtain phylogenetic information, and analyse genetic distances. A neighbour-joining tree was constructed based on the mitochondrial COI gene using the Kimura two-parameter model. This study also exhibits conservation status for those identified species. Our findings will facilitate future studies of fish species diversity in mangrove estuary-based ecosystems and provide preliminary data in policymaking in conservation areas such as National Park.
Isolasi dan Seleksi Bakteri Proteolitik Asal Limbah Cair Tahu Sebagai Dasar Penentuan Agen Pembuatan Biofertilizer Muhammad Asril; Sovia Santi Leksikowati
Elkawnie: Journal of Islamic Science and Technology Vol 5, No 2 (2019)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v5i2.4356

Abstract

Liquid tofu waste has the effect of damaging the environment if treated poorly. The waste contains a reasonably high protein so that it can be used as a substrate for isolation of proteolytic bacteria. Proteases produced by proteolytic bacteria can hydrolyze proteins in liquid tofu waste. The results of protein hydrolysis are amino acids in the form of nitrogen. The nitrogen can be used by plants for nutritional growth so that bioconversion products from proteolytic bacteria can be used as biofertilizers. The purpose of this study was to isolate and select potential proteolytic isolates from tofu liquid waste, which could be used as biofertilizer agents. The method used was bacterial isolation, proteolytic activity test, potato decay test, hemolysis test, and antagonism test among isolates. The results showed that there were two potential isolates from 28 isolates that had been successfully isolated, namely BLT-12 and BLT 21 with each Proteolytic Index of 3.20 and 3.16, respectively. The two isolates were gram-positive in the form of basil, did not have hemolysis activity, could not decompose potatoes so that they did not have a pathogenic effect on the test plants and did not have antagonistic properties between the two others. Therefore, BLT 12 and BLT 21 isolates can be used as candidates for potential isolates of biofertilizer agents.
TRUE SHALLOT ( Allium cepa var ascalonicum ) SEED PRODUCTION DURING OFF SEASON Ramadhani Eka Putra; D Beta Ramadan; Adriyanita Adin; Ida Kinasih; Mia Rosmiati; Indah Oktaviani; Sovia Santi Leksikowati
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 28 No. 2 (2021): BIOTROPIA Vol.28 No.2, Agustus 2021
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.39 KB) | DOI: 10.11598/btb.2021.28.2.1079

Abstract

The application of seed for true shallot cultivation is an alternative of the more common cultivation practice, in which 30% of harvested tubers used for cultivation purposes. The seed production of this temperate tuber, in the tropical region, is quite challenging due to low flowers and seed formation. Several studies showed that vernalization (cold induction) and application of Benzil Amino Purin (BAP) could be applied to improve flowering and seed production. However, such studies were conducted during the best cultivation period for about 3 months and thus, limit the production period of seeds. This study was conducted to observe the effect of both methods outside cultivation periods to flower and capsule numbers, fruit set, and weight of 100 seeds compared with common cultivation. In this study, bulbs of onion vernalized at 10oC for 30 days then became subjected to synthetic hormone (BAP) prior planted while control group The results showed that BAP treated shallot group has the lowest values for all observed parameters (1552.67, 312.11, 22.5%, 0.2244 gram) compared to those vernalization treated group (1592.44, 623, 30.5%; 0.2261 gram) and control group (6774.67; 3898.44; 57.06%; 0.3304 gram). Based on this study, it could be concluded that common cultivation is a better method to produce true shallot seeds during the offseason.
Keanekaragaman Jenis Burung di Kawasan Pengembangan Institut Teknologi Sumatera (ITERA) Indah Oktaviani; Yanti Ariyanti; Sovia Santi Leksikowati; Muhammad Asril
Al-Kauniyah: Jurnal Biologi Vol 14, No 1 (2021): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v14i1.12323

Abstract

AbstrakKawasan kampus Institut Teknologi Sumatera (ITERA) sedang mengalami perkembangan fisik yang pesat, seperti pembangunan gedung perkuliahan, laboratorium, asrama, juga embung untuk sumber air. Informasi mengenai keanekaragaman hayati di ITERA sendiri belum banyak diteliti, salah satunya adalah keanekaragaman burung. Burung merupakan hewan besar yang cukup sensitif dengan perubahan lingkungan. Penelitian ini dilakukan untuk mendapatkan data mengenai keanekaragaman burung di ITERA untuk memberikan informasi dasar. Metode yang digunakan adalah teknik point count, diterapkan pada lima stasiun utama yang dipilih berdasarkan pusat aktivitas pembangunan. Analisis yang digunakan adalah indeks keanekaragaman, kemerataan, juga kelimpahan. Selain itu, status konservasi dan tipe pakan jenis burung dianalisis menggunakan data sekunder. Hasil penelitian menunjukkan terdapat 19 jenis burung dari 16 famili. Keanekaragaman jenis burung berdasarkan indeks Shannon-Wiener tergolong sedang dan kemerataan jenis merata. Dari kategori kelimpahan yang digunakan terdapat dua jenis burung yang umum ditemukan. Bersumber pada PP No. 7 tahun 1999, terdapat empat jenis burung di ITERA yang termasuk ke dalam kategori dilindungi dan satu jenis termasuk kategori Appendix II di CITES. Feeding guild burung di ITERA didominasi tipe omnivora dan insektivora. AbstractInstitut Teknologi Sumatera (ITERA) campus area is undergoing rapid physical development, such as the construction of lecture buildings, laboratories, dormitories, as well as reservoirs for water sources. Information on biodiversity in ITERA has not much studied, one of which is bird diversity. Birds are large animals that are quite sensitive to environmental changes. This study aims to obtain data on bird diversity at ITERA to provide the baseline information. The method used is a point count technique, applied to five main stations selected based on the center of development activities. The analysis used is an index of diversity, evenness, and abundance. Besides, the conservation status and feed type of bird species were analyzed using secondary data. The results showed that there were 19 species of birds from 16 families. The Shannon-Wiener diversity index classified the bird community as a moderate, and the community evenness index was stable. From the abundance category, two types of birds commonly found. Based on PP No. 7 of 1999, there are four species of birds in ITERA, which included in the protected category and one species, including the Appendix II category in CITES. Omnivorous and insectivorous types dominate bird guild feeding in ITERA.
DNA Barcoding for Selected Mangrove-Based Estuary Fishes from Way Kambas National Park, Lampung Province, Indonesia Yanti Ariyanti; Ika Agus Rini; Indah Oktaviani; Sovia Santi Leksikowati
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.04

Abstract

Over the past decade, DNA barcoding has provided new insight into fish ecology and biosystematics and led to new species' discovery. DNA barcoding is a method for the recognition and identification of species using short, standardised DNA fragments. The correct taxonomic identification of species is critical for the assessment and monitoring of biodiversity. This study applied DNA barcoding techniques to identify selected fish species from a mangrove-based estuary in Way Kambas National Park, Lampung Province, Indonesia. The gene encoding cytochrome c oxidase subunit I (COI) was amplified and bi-directionally sequenced from 22 specimens. The resulting 680 base pairs (bp) sequence was used to identify species, obtain phylogenetic information, and analyse genetic distances. A neighbour-joining tree was constructed based on the mitochondrial COI gene using the Kimura two-parameter model. This study also exhibits conservation status for those identified species. Our findings will facilitate future studies of fish species diversity in mangrove estuary-based ecosystems and provide preliminary data in policymaking in conservation areas such as National Park.
A systematic survey of plant biodiversity study within the land of Institut Teknologi Sumatera (ITERA) I. Oktaviani; M. Asril; Y. Aryanti; S. S. Leksikowati
Journal of Science and Applicative Technology Vol 2 No 1 (2019): ICoSITeR Special Edition
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (460.229 KB) | DOI: 10.35472/281474

Abstract

The conversion of agricultural land and plantation into an area with high human activity can affect the biodiversity contained in it. The biodiversity of a region can be surveyed and collect in a systematic database to know the wealth of flora and fauna resources within a certain period of time. The aim of this research is to identify and inventory the vegetation database of plant, which can then be used as one of the important information related to the environmental change that happened from time to time following the impact of the growing development expansion. This study was conducted within the campus of the Institut Teknologi Sumatra (ITERA) which is undergoing physical construction in the form of buildings, roads, and some retention basin. Data retrieval was done by direct observation using the cruising method. The observations of each species are documented, identified, recorded and arranged in the collection catalog.
Aplikasi Trigona (Tetragonula) laeviceps sebagai Agen Penyerbuk pada Sistem Tumpang Sari Buncis dan Tomat di dalam Rumah Kaca LEKSIKOWATI, SOVIA SANTI; EKA PUTRA, RAMADHANI; ROSMIATI, MIA; KINASIH, IDA; ZAKIYATUN USNA, INAYATI; NOVITASARI; SETYARNI, EKA; ANJARNISA RUSTAM, FAJRINA
Jurnal Sumberdaya Hayati Vol. 4 No. 2 (2018)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.4.2.63-70

Abstract

Greenhouse is widely applied to provide protection against pest and harsh environment condition and most farmers usually planted mixed crops inside. However, this system creates an isolation to pollination which responsible for fruit production. In this study, local domesticated stingless bees, Trigona (Tetragonula) laeviceps, was applied as pollinator for two crops, beans (Phaseolus vulgaris L.) and tomatoes (Solanum lycopersicum L.) planted as mixed crops inside greenhouse. The benefit of the bees was measured as pollination success and quality of the crops produced (size and weight). Tomato flowers received no visit from both wild pollinators (all was Apis cerana) and stingless bees while in bean flowers, A. cerana exhibited more and longer visits to flower than T. laeviceps albeit lower number of flowers visited. The pollination efficiency between wild pollinator and T. laeviceps for beans (both at 41%) while self pollination produced better pollination efficiency for tomatoes planted at open field (84% compared to 71%). Beans planted at open field produced better products (bigger size and heavier) while tomatoes produced lesser quality although the differences were not significant. Based on this study, T. laeviceps may applied as pollinator agent in greenhouse and for mixed crops of beans and tomatoes.
Sosialisasi Potensi Etnobotani dan Etnozoologi Desa Pahawang Maretta, Gres; Nurhayu, Winati; Novriadi, Novriadi; Yanti Ariyanti, Yanti Ariyanti; Wahyuni, Kurnia; Riana, Elisa Nurma; Khairani, Iffa Afiqa; Darmawan, Andy; Leksikowati, Sovia Santi; Kesuma, Andri Jaya
Abdimas Galuh Vol 7, No 1 (2025): Maret 2025
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ag.v7i1.17818

Abstract

Etnobiologi adalah evaluasi ilmiah terhadap pengetahuan penduduk tentang biologi, meliputi pengetahuan tentang tumbuhan (etnobotani), hewan (etnozoologi) dan lingkungannya (etnoekologi). Kajian etnobiologi dapat menghubungkan biologi dan etnis yang ada pada suatu tradisi adat. Melalui kegiatan Pengabdian Kepada Masyarakat, mendorong masyarakat untuk peduli terhadap pelestarian sumber daya alam yang ada di Desa Pahawang. Hasil akhir dari pelaksanaan kegiatan adalah pengetahuan masyarakat tentang pemanfaatan etnobotani dan etnozoologi yang ada di desa Pahawang. Kami melakukan penilaian terhadap pengetahuan dan ketertarikan masyarakat mengenai pemanfaatan etnobotani dan etnozoologi yang ada dan diterapkan di desa Pahawang. Pengetahuan masyarakat mengenai potensi dan pemanfaatan etnobotani dan etnozoologi di desa Pahawang meningkat setelah pelatihan jika dibandingkan dengan sebelum pelatihan. Hal ini dapat dilihat dari ketercapaian tujuan dari kegiatan dari hasil pengolahan data kuesioner. Rerata ketercapaian tujuan dapat dikatakan baik (75,6%) berdasarkan dari hasil pre-test dan post-test, dimana terdapat peningkatan pengetahuan dari ibu-ibu peserta kegiatan sebesar dari awal hingga akhir kegiatan. Kemampuan melakukan budidaya tanaman pangan, dan pengetahuan tentang pengobatan alami merupakan potensi besar untuk dikembangkan demi meningkatkan ketahanan pangan dan kesehatan yang lebih baik bagi masyarakat Desa Pahawang ke depan.
Studi etnobotani bahan kerajinan dan bangunan pada Suku Lampung di kabupaten Lampung Barat Sovia Santi Leksikowati; Rahayu, Yeni; Oktaviani, Indah; Ariyanti, Yanti
JURNAL BIOLOGICA SAMUDRA Vol 6 No 2 (2024): Biologica Samudra
Publisher : Program Studi Biologi, Fakultas Teknik, Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jbs.v6i2.10794

Abstract

Suku Lampung memanfaatkan berbagai spesies tumbuhan dan kearifan lokal di kehidupan sehari-hari. Penelitian ini bertujuan menginventarisasi keanekaragaman tumbuhan sebagai bahan kerajinan dan bangunan yang dimanfaatkan oleh Suku Lampung di Kabupaten Lampung Barat. Pengambilan data dilakukan melalui wawancara semi terstruktur dan observasi terhadap Suku Lampung di Pekon Balak, Kecamatan Batu Brak; Pekon Buay Nyerupa dan Pekon Tapak Siring, Kecamatan Sukau; Pekon Way Empulau Ulu, Kecamatan Balik Bukit; dan Pekon Hujung, Kecamatan Belalau, Kabupaten Lampung Barat. Terdapat 7 spesies yang dimanfaatkan sebagai bahan kerajinan yaitu rotan (Calamus sp.), jukuk (Equisetum debile), pelupuh bamban (Schizostachyum bamban), kawokh tali (Gigantochloa apus), pelupuh betung (Dendrocalamus asper), kunyit (Curcuma longa), dan kayu manis (Cinnamomum verum). Bagian tumbuhan yang dimanfaatkan dalam pembuatan kerajinan antara lain batang (5 spesies), rimpang (1 spesies), dan kulit kayu (1 spesies). Sebanyak 5 spesies tumbuhan berperan sebagai bahan utama kerajinan, sedangkan 2 spesies lainnya berperan sebagai pewarna kerajinan. Terdapat 7 spesies tumbuhan sebagai bahan bangunan yaitu medang (Listea sp.), kenitu (Chrysophyllum cainito), cempaka (Michelia champaca), meranti (Shorea sp.), kurut (Dysoxylum parasiticum), pelupuh betung (Dendrocalamus asper), dan aren (Arenga pinnata). Bagian tumbuhan yang dimanfaatkan sebagai bahan bangunan adalah batang (6 spesies) dan serabut pelepah (1 spesies).
Co-Authors Adriyanita Adin Andri Jaya Kesuma ANDY DARMAWAN ANJARNISA RUSTAM, FAJRINA Asril, Muhammad D Beta Ramadan Gres Maretta, Gres I. Oktaviani Ida Kinasih Iffa Afiqa Khairani Ika Agus RINI Ika Agus Rini, Ika Agus Kurnia Wahyuni Muhammad Asril Novitasari Novriadi, Novriadi Nurhayu, Winati Oktaviani, Indah Ramadhani Eka Putra Riana, Elisa Nurma Rosmiati, Mia SETYARNI, EKA Veithzal Rivai Zainal Y. Aryanti Yanti Ariyanti Yanti Ariyanti, Yanti Yanti Ariyanti, Yanti Ariyanti Yeni Rahayu ZAKIYATUN USNA, INAYATI