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Utilising Synthetic Biology to Produce Renewable Energy from Indonesia’s Biodiversity: Inspiration from iGEM Competition Kemal, Rahmat Azhari; Rini, Ika Agus; Oktaviani, Indah
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.209 KB)

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The emerging field of synthetic biology focuses on engineering biomolecular systems and cellular capabilities. It can be applied to a wide variety of applications, one of them is renewable energy production. Teams competing in the International Genetically Engineered Machine (iGEM) competition design and assemble biological devices able to do certain purposes such as producing renewable energy. Their ideas can be adapted by using compatible organisms available from Indonesia’s rich biodiversity. Several ideas are highlighted in this review. One of them is the idea of recycling methanol in glycogen that can be used as carbon source for biofuel production. Methanol dehydrogenase from Indonesia’s methylotrophic bacteria can substitute Bacillus methanolycus used in the system. The adapted ideas can serve as starting points for research, production, and implementation of renewable energy in Indonesia.Keywords: Biodiversity; iGEM; Indonesia; renewable energy; synthetic biology
Isolasi Bakteri Indigineous dari Limbah Cair Tahu dalam Mendegradasi Protein dan Melarutkan Fosfat Asril, Muhammad; Oktaviani, Indah; Leksikowati, Santi
Jurnal Teknologi Lingkungan Vol. 20 No. 1 (2019)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.754 KB) | DOI: 10.29122/jtl.v20i1.3132

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ABSTRACTThe tofu industry in Indonesia is growing rapidly along with the consumption needs. This amount affects the production of the tofu wastewater that will be discharged into the environment. The waste contains high levels of BOD, COD, and high protein organic substances. The discharged of the tofu wastewater without going through a good process of separation and treatment caused odor and pollution in the water and the soil environment. This study aims to examine the amount of production, a chemical characteristic, and bacterial potential (the genus of Pseudomonas and Aeromonas, proteolytic bacterial isolates, and potential phosphate solvent bacteria) which can be used as a reference for bioconversion of higher tofu wastewater. The method used in this study was water sampling, chemical characteristic testing (BOD, COD, total protein, and pH), the bacterial isolation of Pseudomonas and Aeromonas using GSP media, isolation of proteolytic bacteria using nutrient agar + Skim milk media and testing the solubilizing phosphate ability in pikovskaya media. The results showed the production of the tofu liquid waste reached 4000 liters/day with BOD, COD, total protein and pH content is 3210 mg/ L, 7102 mg/L, 20.74% and 5, respectively. The amount of indigenous bacteria in wastewater from Pseudomonas were 72 x 102 cfu/ml), Aeromonas were 18 x 102 cfu/ml), and total proteolytic bacteria were 23.7 x 105 cfu/ml). Proteolytic bacteria obtained in this research were 28 isolates with diverse proteolytic abilities. In addition, 17 of the 28 proteolytic bacteria isolates have the ability to soluble phosphate on pikovskaya medium. These results show that the production of the tofu liquid waste is quite high. Therefore, the chemical character and the presence of bacteria in it have the potential to be developed and can be used as a reference for bioconversion of waste before being discharged into the environment.Keywords: Aeromonas, proteolytic bacteria, tofu wastewater, phosphate solubilizing bacteria, Pseudomonas ABSTRAKIndustri tahu di Indonesia berkembang sangat pesat seiring dengan kebutuhan konsumsi masyarakat Indonesia. Jumlah ini berpengaruh terhadap produksi limbah cair tahu yang akan dibuang ke lingkungan. Limbah tersebut memiliki kandungan BOD, COD dan bahan organik berupa protein yang cukup tinggi. Limbah cair tahu jika dibuang lingkungan tanpa melalui proses pemisahan dan perlakuan yang baik dapat menimbulkan bau dan pencemaran di lingkungan air dan tanah. Penelitian ini bertujuan untuk mengkaji jumlah produksi, karakteristik kimia dan potensi biologi (bakteri genus Pseudomonas dan Aeromonas, isolat bakteri proteolitik dan bakteri pelarut fosfat potensial) yang nantinya dapat digunakan sebagai acuan biokonversi limbah cair tahu yang semakin tinggi. Metode yang digunakan pada penelitian ini adalah pengambilan sampel, pengujian karakteristik kimia (BOD, COD, total protein dan pH), isolasi bakteri genus Pseudomonas dan Aeromonas pada media GSP, isolasi bakteri proteolitik di media Nutrien agar + Skim milk dan pengujian kemampuan melarutkan fosfat pada media pikovskaya. Hasil menunjukkan bahwa limbah cair yang diproduksi setiap harinya mencapai 4000 liter/hari dengan kandungan BOD, COD, total protein dan pH berturut-turut sebesar 3210 mg/L, 7102 mg/L, 20.74 % dan 5. Jumlah bakteri indigeous dalam limbah cair tahu dari genus Pseudomonas adalah 72 x 102 cfu/ml, genus Aeromonas adalah 18 x 102 cfu/ml dan total bakteri proteolitik adalah 23.7 x 105 cfu/ml. Bakteri proteolitik yang diperoleh berjumlah 28 isolat dengan kemampuan proteolitik yang cukup beragam. Selain itu, 17 dari 28 isolat bakteri proteolitik tersebut memiliki kemampuan melarutkan fosfat pada media pikovskaya. Hasil ini menunjukkan bahwa produksi limbah cair tahu yang cukup tinggi, karakteristik kimia serta keberadaan bakteri didalamnya memiliki potensi untuk dikembangkan dan dapat dijadikan sebagai acuan untuk biokonversi limbah sebelum dibuang ke lingkungan.Kata kunci: Aeromonas, bakteri proteolitik, limbah cair tahu, bakteri pelarut fosfat, Pseudomonas
Tracking The Source of Antimicrobial Production From House Fly (Musca domestica): Right-Wing of Fly Or Gut System? - A Mini-Review Muhammad Asril; Ika Agus Rini; Indah Oktaviani; Mushaliyah Mushaliyah
Elkawnie: Journal of Islamic Science and Technology Vol 7, No 2 (2021)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i2.9495

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Abstract: The house fly (Musca domestica) is a vector of disease-causing bacteria because of its habit of perching and feeding on various substrates of pathogenic bacteria.  His role as a disease carrier contradicts the hadith narrated by Bukhari, which is "If a fly falls into your vessel, drown it and then remove it because one of its wings carries disease and the other is the cure". This hadith indicates the presence of antimicrobial compounds produced from the body of flies. Various research reports show that the truth of this hadith is that there are antimicrobial-producing bacterial symbionts on the wings of flies (left and right) that can kill pathogenic bacteria on one of the wings or both. Antimicrobial compounds are also produced naturally in the digestive tract of flies from the larval stage to adulthood as a response to the body's defense against the presence of pathogenic bacteria in their bodies. The antimicrobial compounds are lysozyme, defensin, cecropin, diptericin, and several antimicrobial peptide compounds. This compound can also be removed mechanically through pressure (the process of immersing the fly's body in water). This shows that the process of drowning aims to extract or release antimicrobial compounds from the digestive tract of flies to neutralize pathogenic bacteria that have mixed in the liquid in specific containers. This review aims to examine various reports related to antimicrobial substances produced in flies and their evidence in this hadith.Abstrak: Lalat rumah (Musca domestica) merupakan vektor pembawa bakteri penyebab penyakit karena kebiasaan hinggap dan makan pada berbagai substrat bakteri patogen. Perannya sebagai pembawa penyakit memiliki kontradiksi dengan hadist yang diriwayatkan oleh Bukhari yaitu “Jika seekor lalat jatuh ke bejana kamu, tenggelamkanlah kemudian singkirkan, karena salah satu sayapnya membawa penyakit dan sayap lainnya adalah obatnya”. Hadist ini mengindikasikan adanya senyawa antimikroba yang dihasilkan dari tubuh lalat. Berbagai laporan penelitian menunjukkan bahwa kebenaran hadist ini yaitu pada sayap lalat (kiri dan kanan) terdapat bakteri simbion penghasil antimikrob yang mampu membunuh bakteri patogen pada salah satu sayap atau keduanya. Senyawa antimikrob juga dihasilkan secara alami dalam pencernaan lalat sejak tahap larva hingga dewasa sebagai respon pertahanan tubuh terhadap keberadaan bakteri patogen ditubuhnya. Senyawa antimikrob tersebut berupa lisozim, defensin, cecrofin, diptericin dan beberapa senyawa peptida antimikrob. Senyawa ini juga dapat dikeluarkan secara mekanik melalui tekanan (proses penenggelaman tubuh lalat dalam air). Hal ini menunjukkan bahwa proses penenggelaman lalat kedalam air bertujuan untuk mengekstrak atau mengeluarkan senyawa antimikrob dari pencernaan lalat untuk menetralisir bakteri patogen yang telah bercampur dalam cairan di wadah tertentu. Review ini bertujuan untuk mengkaji berbagai laporan terkait senyawa antimikrob yang dihasilkan pada tubuh lalat dan pembuktiannya pada hadist tersebut.
TRUE SHALLOT ( Allium cepa var ascalonicum ) SEED PRODUCTION DURING OFF SEASON Ramadhani Eka Putra; D Beta Ramadan; Adriyanita Adin; Ida Kinasih; Mia Rosmiati; Indah Oktaviani; Sovia Santi Leksikowati
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 28 No. 2 (2021): BIOTROPIA Vol.28 No.2, Agustus 2021
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.39 KB) | DOI: 10.11598/btb.2021.28.2.1079

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The application of seed for true shallot cultivation is an alternative of the more common cultivation practice, in which 30% of harvested tubers used for cultivation purposes. The seed production of this temperate tuber, in the tropical region, is quite challenging due to low flowers and seed formation. Several studies showed that vernalization (cold induction) and application of Benzil Amino Purin (BAP) could be applied to improve flowering and seed production. However, such studies were conducted during the best cultivation period for about 3 months and thus, limit the production period of seeds. This study was conducted to observe the effect of both methods outside cultivation periods to flower and capsule numbers, fruit set, and weight of 100 seeds compared with common cultivation. In this study, bulbs of onion vernalized at 10oC for 30 days then became subjected to synthetic hormone (BAP) prior planted while control group The results showed that BAP treated shallot group has the lowest values for all observed parameters (1552.67, 312.11, 22.5%, 0.2244 gram) compared to those vernalization treated group (1592.44, 623, 30.5%; 0.2261 gram) and control group (6774.67; 3898.44; 57.06%; 0.3304 gram). Based on this study, it could be concluded that common cultivation is a better method to produce true shallot seeds during the offseason.
Keanekaragaman Jenis Burung di Kawasan Pengembangan Institut Teknologi Sumatera (ITERA) Indah Oktaviani; Yanti Ariyanti; Sovia Santi Leksikowati; Muhammad Asril
Al-Kauniyah: Jurnal Biologi Vol 14, No 1 (2021): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v14i1.12323

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AbstrakKawasan kampus Institut Teknologi Sumatera (ITERA) sedang mengalami perkembangan fisik yang pesat, seperti pembangunan gedung perkuliahan, laboratorium, asrama, juga embung untuk sumber air. Informasi mengenai keanekaragaman hayati di ITERA sendiri belum banyak diteliti, salah satunya adalah keanekaragaman burung. Burung merupakan hewan besar yang cukup sensitif dengan perubahan lingkungan. Penelitian ini dilakukan untuk mendapatkan data mengenai keanekaragaman burung di ITERA untuk memberikan informasi dasar. Metode yang digunakan adalah teknik point count, diterapkan pada lima stasiun utama yang dipilih berdasarkan pusat aktivitas pembangunan. Analisis yang digunakan adalah indeks keanekaragaman, kemerataan, juga kelimpahan. Selain itu, status konservasi dan tipe pakan jenis burung dianalisis menggunakan data sekunder. Hasil penelitian menunjukkan terdapat 19 jenis burung dari 16 famili. Keanekaragaman jenis burung berdasarkan indeks Shannon-Wiener tergolong sedang dan kemerataan jenis merata. Dari kategori kelimpahan yang digunakan terdapat dua jenis burung yang umum ditemukan. Bersumber pada PP No. 7 tahun 1999, terdapat empat jenis burung di ITERA yang termasuk ke dalam kategori dilindungi dan satu jenis termasuk kategori Appendix II di CITES. Feeding guild burung di ITERA didominasi tipe omnivora dan insektivora. AbstractInstitut Teknologi Sumatera (ITERA) campus area is undergoing rapid physical development, such as the construction of lecture buildings, laboratories, dormitories, as well as reservoirs for water sources. Information on biodiversity in ITERA has not much studied, one of which is bird diversity. Birds are large animals that are quite sensitive to environmental changes. This study aims to obtain data on bird diversity at ITERA to provide the baseline information. The method used is a point count technique, applied to five main stations selected based on the center of development activities. The analysis used is an index of diversity, evenness, and abundance. Besides, the conservation status and feed type of bird species were analyzed using secondary data. The results showed that there were 19 species of birds from 16 families. The Shannon-Wiener diversity index classified the bird community as a moderate, and the community evenness index was stable. From the abundance category, two types of birds commonly found. Based on PP No. 7 of 1999, there are four species of birds in ITERA, which included in the protected category and one species, including the Appendix II category in CITES. Omnivorous and insectivorous types dominate bird guild feeding in ITERA.
PENGENALAN MEDIA PEMBELAJARAN DARING BERBASIS STEM UNTUK GURU IPA DI SMAN 9 BANDAR LAMPUNG Indah Oktaviani; Ika Agus Rini; Mia Maria Ulfah; Ahmad Dodi Andriana
Jubaedah : Jurnal Pengabdian dan Edukasi Sekolah (Indonesian Journal of Community Services and School Education) Vol. 1 No. 1 (2021): Jurnal Pengabdian dan Edukasi Sekolah (Jubaedah)
Publisher : LPPM Universitas Bina Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46306/jub.v1i1.16

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Meluasnya pandemi Covid-19 memaksa proses pembelajaran di sekolah dilakukan secara daring. Hal ini menuntut guru untuk lebih kreatif dalam menggunakan media pembelajaran yang berbasis teknologi agar pembejaran lebih interaktif terutama pada kondisi pandemi. Akan tetapi tidak banyak guru di sekolah yang menguasi perangkat lunak sebagai media pembejaran, sehingga perlu dilakukan pelatihan dalam pemanfaatan teknologi dalam Pembelajaran Jarak Jauh. Metode yang dilakukan antara lain perancangan dan persiapan yang meliputi survei terhadap kondisi guru dalam memanfaatkan media pembelajaran, pelaksanaan kegiatan, dan evaluasi. Hasil survei awal menunjukkan bahwa guru IPA masih sangat terbatas dalam memanfaatkan metode pembelajaran dengan perangkat lunak interaktif, sehingga dilakukan pelatihan dengan modul yang sudah disediakan dan perangkat lunak yang dapat mewakili pembelajaran IPA berbasis STEM dengan penggunaan Nearpod dan Laboratorium virtual simulasi PhET. Hasil Pelatihan menunjukkan adanya peningkatan pemahaman dan kreatifitas guru IPA dalam proses pembelajaran daring
ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL IAA (INDOLE ACETIC ACID) DARI RHIZOSFER TANAMAN AKASIA (Acacia mangium) Ika Agus Rini; Indah Oktaviani; Muhammad Asril; Revi Agustin; Fina Khaerunissa Frima
Agro Bali : Agricultural Journal Vol 3, No 2 (2020)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.046 KB) | DOI: 10.37637/ab.v3i2.619

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IAA adalah produk paling umum dari metabolisme L-triptofan yang dapat diproduksi oleh beberapa mikroorganisme. Beberapa mikroorganisme yang memiliki potensi menghasilkan IAA adalah bakteri rhizosfer pada tanaman Leguminosae, salah satunya adalah akasia. Acacia mangium, juga dikenal sebagai akasia, adalah pohon yang tumbuh cepat. Namun, akasia adalah tanaman invasif. Tanaman akasia memiliki bintil yang merupakan hasil simbiosis akar tanaman dan bakteri. Simbiosis ini dapat mempengaruhi kesuburan tanah. Banyak potensi yang dapat digali dari bakteri tanah, khususnya di rhizosfer. Tujuan dari penelitian ini adalah untuk mengisolasi dan mengidentifikasi bakteri pada rizosfer tanaman akasia yang mampu menghasilkan IAA sebagai salah satu potensi untuk kandidat PGPR (Plant Growth Promoting Rhizobacteria). Metode yang digunakan meliputi pengambilan sampel, isolasi bakteri penghasil IAA, pemurnian bakteri, identifikasi bakteri dan uji biokimia, pembuatan kurva tumbuha bakteri, dan uji isolat bakteri pelarut fosfat. Hasil isolasi bakteri rhizosfer diperoleh sebanyak 10 isolat bakteri yang memiliki karakteristik berbeda secara morfologi.  Berdasarkan hasil identifikasi bakteri berdasarkan pewarnaan Gram, bakteri tersebut masuk ke dalam genus Bacillus dan terdapat 5 isolat bakteri yang memiliki kemampuan menghasilkan IAA dan melarutkan fosfat sehingga bakteri tersebut memiliki potensi sebagai pupuk hayati.
DNA Barcoding for Selected Mangrove-Based Estuary Fishes from Way Kambas National Park, Lampung Province, Indonesia Yanti Ariyanti; Ika Agus Rini; Indah Oktaviani; Sovia Santi Leksikowati
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.04

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Over the past decade, DNA barcoding has provided new insight into fish ecology and biosystematics and led to new species' discovery. DNA barcoding is a method for the recognition and identification of species using short, standardised DNA fragments. The correct taxonomic identification of species is critical for the assessment and monitoring of biodiversity. This study applied DNA barcoding techniques to identify selected fish species from a mangrove-based estuary in Way Kambas National Park, Lampung Province, Indonesia. The gene encoding cytochrome c oxidase subunit I (COI) was amplified and bi-directionally sequenced from 22 specimens. The resulting 680 base pairs (bp) sequence was used to identify species, obtain phylogenetic information, and analyse genetic distances. A neighbour-joining tree was constructed based on the mitochondrial COI gene using the Kimura two-parameter model. This study also exhibits conservation status for those identified species. Our findings will facilitate future studies of fish species diversity in mangrove estuary-based ecosystems and provide preliminary data in policymaking in conservation areas such as National Park.
Deskripsi Morfologi Penyakit pada Batang Buah Naga (Hylocereus sp.) dan Pengendaliannya Menggunakan Pestisida Nabati dari Serai Wangi (Cymbopogon nardus) Indah Oktaviani; Ayu Octavia Tanjung Putri; Mesita Dwi Pebina
Bioscientist : Jurnal Ilmiah Biologi Vol 10, No 2 (2022): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v10i2.6125

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Dragon fruit (Hylocereus sp.) is a plant introduced from America and started to be cultivated in several areas in Indonesia. The high consumer demand is one reason to increase dragon fruit production. However, during the growth period, dragon fruit plant stems are at high risk for disease. This study aims to describe the stem morphology of dragon fruit plants that are attacked by disease and to determine the effect of vegetable pesticides from Lemongrass Wangi (Cymbopogon nardus) in the cultivation area of the Lampung Province Agricultural Training Center (BPP). The research was divided into two stages, namely field observations using survey methods, and testing the application of vegetable pesticides on diseased plants. Observations made in the first stage included: stem morphological symptoms, disease attack frequency (FS), and disease attack intensity (IS). Observations on stem morphology showed that there were eight types of disease with different symptoms and causes, with an attack frequency of up to 100%. The highest percentage of disease attack intensity was caused by anthracnose (56.7%), and the lowest was brown rot (13.4%). Disease control trials using botanical pesticides were carried out on three diseases that attack almost all individual dragon fruit plants, namely: anthracnose, mosaic, and stem rot. The results showed a decrease in attack intensity on stem rot, mosaic, and anthracnose after four weeks of pesticide spraying.
Design of E. Coli Systems as Iron Waste Binders for Wastewater Treatment using Synthetic Biology Approach Indah Oktaviani
Journal of Science and Applicative Technology Vol 2 No 2 (2018): Journal of Science and Applicative Technology December Chapter
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (569.066 KB) | DOI: 10.35472/281443

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The development of recombinant DNA technology and the emerging field of synthetic biology have open up a more advanced era of biotechnology. The discoveries are developed to solve the existing problems in the community.The presence of the iGEM competition initiated by the iGEM Foundation since 2003 adding up new data and a new discovery that is constantly being improved upon, so that peoples’ interest and concern with synthetic biology in the world are increasing every year, including in Indonesia.This paper discusses the idea of design to develop an expression system with a synthetic biology approach using standard parts listed on the iGEM website.The system design uses Escherichia coli as its chassis to have the capability as an iron waste bioaccumulator agent in the waters. This draft is expected to be one of the contributions for the future of the development of synthetic biology ideas, research, and applications in handling environmental problems in Indonesia.