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Akollo, Isak Roberth
Fakultas Kesehatan, Universitas Kristen Indonesia Maluku
Biochemistry, Genetics & Molecular Biology Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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Status Resistensi Nyamuk Aedes aegypti terhadap Malation dan Mutasi Gen Ace-1 di Kota Ambon Isak Roberth Akollo; Tri Baskoro Satoto; Sitti Rahmah Umniyati
Jurnal Vektor Penyakit Vol 14 No 2 (2020): Edisi Desember
Publisher : Balai Penelitian dan Pengembangan Kesehatan Donggala, Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/vektorp.v14i2.2934

Abstract

Abstract Aedes aegypti is the main vector of Dengue Hemorrhagic Fever (DHF). The continuous use of insecticides can cause resistant Ae. aegypti. One indicator of mosquito resistance to organophosphate is acetylcholinesterase insensitivity, which is encoded by the ace-1 gene. This study aims to analyze the status of resistance and detect ace-1 gene mutations in Ae. aegypti in Ambon city. This research is a descriptive and analytic study with a cross-sectional research design. Resistance status was tested by the CDC bottle bioassay and the detection of ace-1 gene mutations was established using PCR and sequencing. The nucleotide sequence was edited with Mega 7 software. The results of resistance tests using the CDC bottle bioassay method showed Ae. aegypti from the Waihaong and Rijali Community Health Centers had a mortality of 92% and 95.83% (tolerant), while the Latuhalat, Amahusu, Air Salobar, Benteng, Urimessing, Christina Martha Thiahahu, Karpan, Belso, Air Besar, Kilang, Halong, Lateri, Passo, and Nania had mortality between 98-100% (vulnerable). Sequencing and alignment results showed that there were no ace-1 gene mutations in Ae. aegypti originating from Waihaong and Rijali Community Health Centers. Ae. aegypti resistance status in 2 Community Health Centers is tolerant and 14 other Community Health Centers are vulnerable. There are no ace-1 gene mutations in Ae. aegypti at the Waihaong and Rijali Community Health Centers in Ambon City. Abstrak Nyamuk Aedes aegypti adalah vektor utama penyakit demam berdarah dengue (DBD). Penggunaan insektisida terus menerus dapat menyebabkan nyamuk Ae. aegypti resisten. Salah satu indikator resistensi nyamuk terhadap insektisida organofosfat adalah insensitivitas acetylcholinesterase, yang dikode oleh gen ace-1. Penelitian ini bertujuan menganalisis status resistensi dan mendeteksi mutasi gen ace-1 pada nyamuk Ae. aegypti di kota Ambon. Penelitian ini merupakan penelitian deskriptif dan analitik dengan rancangan penelitian cross sectional. Status resistensi diuji dengan metode CDC bottle bioassay dan deteksi mutasi gen ace-1 ditegakkan menggunakan PCR dan sekuensing. Urutan nukleotida diedit dengan software Mega 7. Hasil uji resistensi dengan metode CDC bottle bioassay menunjukan nyamuk Ae. aegypti dari Puskesmas Waihaong dan Rijali memiliki mortalitas 92% dan 95,83% (toleran), sedangkan Puskesmas Latuhalat, Amahusu, Air Salobar, Benteng, Urimessing, Chrsitina Martha Thiahahu, Karpan, Belso, Air Besar, Kilang, Halong, Lateri, Passo, dan Nania memiliki mortalitas antara 98-100% (rentan). Hasil sekuensing dan alignment menunjukan tidak terdapat mutasi gen ace-1 pada nyamuk Ae. aegypti yang berasal dari Puskesmas Waihaong dan Rijali. Status resistensi nyamuk Ae. aegypti di 2 Puskesmas Kota Ambon tergolong toleran dan 14 Puskesmas lainnuya tergolong rentan. Tidak terdapat mutasi gen ace-1 pada nyamuk Ae. aegypti di Puskesmas Waihaong dan Rijali Kota Ambon.
FAKTOR-FAKTOR YANG BERHUBUNGAN DENGAN LOW BACK PAIN DI WILAYAH KERJA PUSKESMAS WAMLANA KABUPATEN BURU Vernando Yanry Lameky; Isak Roberth Akollo; Oci Tasijawa
Molucca Medica Vol 16 No 1 (2023): VOLUME 16, NOMOR 1, APRIL 2023
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/molmed.2023.v16.i1.80

Abstract

Low back pain merupakan gejala yang di tandai dengan nyeri di daerah punggung bagian bawah dan bisa menyebar sampai ke daerah sekitar. Faktor penyebabnya antara lain usia, masa kerja, posisi duduk, lama kerja, Semakin bertambahnya usia, fungsi tubuh akan mengalami penurunan. Waktu berkerja yang lama, dengan posisi duduk yang salah dapat menimbulkan nyeri pada dearah punggung bawah. Tujuan: Untuk mengetahui faktor-faktor yang berhubungan dengan low back pain di wilayah kerja Puskesmas Wamlana kabupaten buru. Deskriptif analitik dengan rancangan cross sectional. Sampel berjumlah 34 responden yang diambil menggunakan Teknik total sampling. Berdasarkan uji Fisher exact terdapat hubungan antara low back pain dengan usia (p=0,048), masa kerja (p=0,029), posisi duduk (p=0,015) dan lama kerja (pe=0,006).Anjuran untuk penderita ialah periksakan kesehatan secara berkala sedangkan untuk lembaga kesehatan agar terus melaksanakan aksi pengobatan melalui asuhan keperawatan serta promosi kesehatan secara komperhensif.Penderita low back pain di sarankan untuk lebih banyak membuat jadwal kerja agar tidak terlalu lama karena sangat memperburuk kondisi.
PENGARUH PERILAKU MEROKOK DAN PEMAKAIAN OBAT NYAMUK BAKAR TERHADAP KEJADIAN ISPA PADA BALITA DI WILAYAH KERJA PUSKESMAS JAZIRAH TENGGARA Syulce Luselya Tabalawony; Isak Roberth Akollo
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 15 No 1 (2023): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v15i1.2216

Abstract

Infeksi saluran pernapasan (ISPA) merupakan penyakit infeksi yang menyerang sistem pernapasan. Penyakit ini masih menjadi masalah kesehatan di dunia termasuk Indonesia. Penyakit ini paling banyak diderita oleh balita. Beberapa faktor yang berpengaruh terhadap munculnya penyakit ISPA pada balita adalah perilaku merokok dan penggunaan obat nyamuk bakar. Penelitian ini bertujuan adalah mengetahui hubungan perilaku merokok dan pemakaian obat nyamuk bakar dengan kejadian ISPA pada balita di Wilayah Kerja Puskesmas Jazirah Tenggara. Desain penelitian yang digunakan dalam penelitian ini adalah cross sectional. Pengumpulan data dalam penelitian ini menggunakan kuesioner. Analisis data dalam penelitian ini menggunakan chi-square test. Hasil penelitian menunjukan bahwa terdapat hubungan perilaku merokok dengan kejadian ISPA dengan nilai p-value sebesar 0.000 dan terdapat hubungan pemakaian obat nyamuk bakar dengan kejadian ISPA dengan nilai p-velue sebesar 0.000. Kesimpulan dari penelitian ini adalah terdapat hubungan antara perilaku merokok dan pemakaian obat nyamuk bakar dengan kejadian ISPA pada balita di Wilayah Kerja Puskesmas Jazirah Tenggara.
PENGARUH PERILAKU MEROKOK DAN PEMAKAIAN OBAT NYAMUK BAKAR TERHADAP KEJADIAN ISPA PADA BALITA DI WILAYAH KERJA PUSKESMAS JAZIRAH TENGGARA Syulce Luselya Tabalawony; Isak Roberth Akollo
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 15 No 1 (2023): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v15i1.2216

Abstract

Infeksi saluran pernapasan (ISPA) merupakan penyakit infeksi yang menyerang sistem pernapasan. Penyakit ini masih menjadi masalah kesehatan di dunia termasuk Indonesia. Penyakit ini paling banyak diderita oleh balita. Beberapa faktor yang berpengaruh terhadap munculnya penyakit ISPA pada balita adalah perilaku merokok dan penggunaan obat nyamuk bakar. Penelitian ini bertujuan adalah mengetahui hubungan perilaku merokok dan pemakaian obat nyamuk bakar dengan kejadian ISPA pada balita di Wilayah Kerja Puskesmas Jazirah Tenggara. Desain penelitian yang digunakan dalam penelitian ini adalah cross sectional. Pengumpulan data dalam penelitian ini menggunakan kuesioner. Analisis data dalam penelitian ini menggunakan chi-square test. Hasil penelitian menunjukan bahwa terdapat hubungan perilaku merokok dengan kejadian ISPA dengan nilai p-value sebesar 0.000 dan terdapat hubungan pemakaian obat nyamuk bakar dengan kejadian ISPA dengan nilai p-velue sebesar 0.000. Kesimpulan dari penelitian ini adalah terdapat hubungan antara perilaku merokok dan pemakaian obat nyamuk bakar dengan kejadian ISPA pada balita di Wilayah Kerja Puskesmas Jazirah Tenggara.
Minyak Atsiri Daun Cengkeh (Eugenia Caryophyllus) Terbukti Menurunkan Intensitas Nyeri Gigi Lameky, Vernando Yanry; Siahaya, Griennasty; Tandi, Dan; Maitimu, Anatji Naomi; Akollo, Isak Roberth
Jurnal Penelitian Kesehatan SUARA FORIKES 2023
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf14nk105

Abstract

At present, some people lack the awareness to have regular dental and oral health checks. One of the non-pharmacological treatments that can be given is using clove leaf essential oil (Eugenia caryophyllus). So research is needed which aims to determine the effect of clove leaf essential oil (Eugenia caryophyllus) on reducing the intensity of tooth pain in the community in the dental polyclinic room of M. Haulussy Hospital Ambon. This study applied a pretest-posttest with control group design. Respondents were 30 patients selected by purposive sampling technique, which were divided into 2 groups. Pain was measured using the Numeric Rating Scale, then analyzed using the t test. The results of the analysis showed differences in pain levels before and after being given clove leaf essential oil (p = 0.000) in the intervention group, whereas in the control group the value was p = 0.719. It was concluded that clove leaf essential oil can be used as evidence based practice and an efficient and effective therapeutic innovation in reducing the intensity of tooth pain in all health care communities, the pharmaceutical industry uses it as an anti-pain drug, anti-infection, bacteria killer, while clove farmers can increase significant economic value.Keywords: clove leaves; eugenia caryophyllus; toothache; essential oil ABSTRAK Saat ini sebagian masyarakat kurang memiliki kesadaran untuk memeriksakan kesehatan gigi dan mulut secara rutin. Salah satu perawatan non farmakologi yang bisa diberikan adalah menggunakan minyak atsiri daun cengkeh (Eugenia caryophyllus). Maka diperlukan penelitian yang bertujuan untuk mengetahui pengaruh minyak atsiri daun cengkeh (Eugenia caryophyllus) terhadap penurunan intensitas nyeri gigi pada masyarakat di ruang poliklinik gigi RSUD M. Haulussy Ambon. Penelitian ini menerapkan pretest-posttest with control group design. Responden adalah 30 pasien yang dipilih dengan teknik purposive sampling, yang dibagi menjadi 2 kelompok. Nyeri diukur menggunakan Numeric Rating Scale, lalu dianalisis menggunakan uji t. Hasil analisis menunjukkan perbedaan tingkat nyeri sebelum dan sesudah diberikan minyak atsiri daun cengkeh (p = 0,000) pada kelompok intervensi, sedangkan pada kelompok kontrol nilai p = 0,719. Disimpulkan bahwa minyak atsiri daun cengkeh dapat dijadikan evidance based practice dan inovasi terapi yang efisien serta efektif dalam menurukan intensitas nyeri gigi pada masyarakat di seluruh pelayanan kesehatan, industri farmasi menggunakannya sebagai obat anti nyeri, anti infeksi, pembunuh bakteri, sedangkan pada petani cengkeh dapat meningkatkan nilai ekonomi secara signifikan.Kata kunci: daun cengkeh; eugenia caryophyllus; nyeri gigi; minyak atsiri
Efektivitas Minyak Atsiri Daun Cengkeh (Syzygium aromaticum) dan Biji Pala (Myristica fragrans) terhadap Low Back Pain: Studi Perbandingan di Kota Ambon Lameky, Vernando Yanry; Akollo, Isak Roberth; Tiwery, Rosdiana Tisye
Jurnal Keperawatan Florence Nightingale Vol 7 No 2 (2024)
Publisher : Sekolah Tinggi Ilmu Kesehatan Stella Maris Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52774/jkfn.v7i2.267

Abstract

Low back pain is a global health problem that affects quality of life and work productivity. The causes vary, from injuries to degenerative diseases. One alternative approach to relieve LBP is clove leaves and nutmeg. Clove leaves contain eugenol, which has anti-inflammatory, analgesic, and muscle relaxant properties, while nutmeg contains myristicin and essential oils that effectively relieve pain. The combination of the two, which can be used in the form of massage oil or herbal ointment, has the potential to provide a synergistic effect in reducing pain and inflammation. This study aims to analyze the effectiveness of clove leaf and nutmeg essential oils in reducing LBP pain in Ambon City. The research method used was quasi-experimental with a pretest-posttest design involving 50 respondents who experienced LBP and were divided into two groups. Data were collected using a numeric pain assessment scale and analyzed using the Paired Sample T-Test. The results showed that 64% of respondents in the clove leaf oil group and 76% in the nutmeg seed oil group experienced moderate pain before the intervention. After the intervention, a significant decrease in pain was found, where the clove leaf group showed an average decrease in pain from 2.36 to 0.24 (p = 0.000), while the nutmeg group from 2.24 to 1.48 (p = 0.001). This study indicates that clove leaf essential oil is more effective in relieving LBP pain than nutmeg seed oil. These findings provide recommendations for using local natural resources as an alternative non-pharmacological therapy in managing LBP.
Penyuluhan tentang Kesehatan Gigi dan Mulut, Perawatan Gigi dan Mulut dan Obat Tradisional dari Daun Cengkeh Lameky, Vernando Yanry; Akollo, Isak Roberth; Djarami, Jayanti; Kolibonso, Billy Franscois; Pattinasarany, Devan Hendrik
Empowerment Vol. 6 No. 03 (2023): Empowerment
Publisher : Program Studi Ilmu Hukum Fakultas Hukum Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/empowerment.v6i03.8793

Abstract

Clove leaves can be processed into essential oils which have various benefits. One of the benefits is overcoming teethache. Clove leaves are still considered waste by the community and have not been utilized. The results of discussions with partners are 3 urgent problems that must be resolved in Community Partnership Empowerment (CPE), namely: 1) Lack of knowledge related to dental and oral health, 2) Lack of public knowledge related to dental care, 3) Lack of knowledge related to traditional medicinal plant communities and utilization of clove leaf waste. So this CPE aims to train partners to innovate essential oil products made from clove leaf waste to treat toothache. The method used in this CPE is counseling and training. The results of the CPE show that people who have participated in the CPE experience increased knowledge about dental and oral health, dental and oral care, and traditional medicine.Daun cengkeh dapat diolah menjadi minyak atsiri yang memiliki berbagai manfaat. Salah satu manfaatnya adalah mengatasi nyeri gigi. Daun cengkeh masih dianggap sampah oleh masyarakat dan belum dimanfaatkan. Hasil diskusi dengan mitra ada 3 masalah urgensi yang harus di selesaikan dalam Pemberdayaan Kemitraan Masyarakat (PKM), yaitu : 1) kurangnya pengetahuan masyarakat terkait kesehatan gigi dan mulut, 2) kurangnya pengetahuan masyarakat terkait perawatan gigi, 3) kurangnya pengetahuan masyarakat terkait tanaman obat tradisional dan pemanfaatan limbah daun cengkeh. Sehingga PKM ini bertujuan untuk melatih mitra melakukan inovasi produk minyak atsiri berbahan limbah daun cengkeh untuk mengatasi nyeri gigi. Metode yang digunakan dalam PKM ini adalah penyuluhan. Hasil PKM menujunkan bahwa masyarakat yang telah mengikuti PKM mengalami peningkatan pengetahunan tentang kesehatan gigi dan mulut, perawatan gigi dan mulut, dan obat tradisional. 
Pelatihan Pembuatan Produk Minyak Atsiri Berbahan Limbah Daun Cengkeh untuk Mengatasi Nyeri Gigi Lameky, Vernando Yanry; Akollo, Isak Roberth; Djarami, Jayanti; Kolibonso, Billy Franscois; Pattinasarany, Devan Hendrik
Empowerment Vol. 7 No. 01 (2024): Empowerment
Publisher : Program Studi Ilmu Hukum Fakultas Hukum Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/empowerment.v7i01.9143

Abstract

Teeth that are not well cared for can cause toothache. Tooth pain can interfere daily human activities. Tooth pain can be treated by traditional medicine. One plant that can be processed to treat toot pain is clove leaves. Clove leaves can be processed into essential oil which is useful in treating toothache. The results of discussions with the Mahia Hamlet community show that there are several problems that must be resolved in the Community Partnership Empowerment (CPE) program. These problems are 1) the lack of community skills to process traditional medicinal plants, clove leaf waste through the distillation process. 2) lack of knowledge and skills in using appropriate technology (TTG), especially kettles (distillation equipment) to produce essential oils. This CPE activity aims to increase knowledge and skills in using appropriate technology, especially kettles (distillation equipment) to produce essential oils. The CPE results show that the community has knowledge and skills about how to distill clove leaf essential oil which can be used in the treatment of toothache.
Sosialisasi Healing Touch sebagai Terapi Non-Farmakologis untuk Nyeri Pasca Imunisasi pada Anak Lameky, Vernando Yanry; Akollo, Isak Roberth; Patty, Joanna Cristy; Tuhuleruw, Bredlcy Fransiscus; Tomasila, Boy
Empowerment : Jurnal Pengabdian Masyarakat Vol. 8 No. 01 (2025): Empowerment
Publisher : Program Studi Ilmu Hukum Fakultas Hukum Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/empowerment.v8i01.10956

Abstract

Immunization is crucial for protecting individuals from vaccine-preventable diseases, but pain during and after vaccination can lead to fear and anxiety in children, causing them to refuse subsequent vaccinations. This study aimed to address the lack of knowledge among parents regarding non-pharmacological pain management techniques, specifically healing touch (HT), through a community empowerment partnership (PKM) program. The PKM was conducted as a socialization event for posyandu cadres at the Waihoka Community Health Center on October 30, 2024. Participants completed a pre-test questionnaire, attended a presentation on HT for post-immunization pain management, engaged in a question-and-answer session, and completed a post-test questionnaire. The results showed that before the socialization, most cadres had poor knowledge of non-pharmacological therapies and HT. However, after the event, their knowledge significantly improved, with the majority demonstrating very good understanding. The study concludes that the socialization effectively enhanced the posyandu cadres' knowledge of HT as a non-pharmacological therapy for post-immunization pain management. The authors recommend that the Ambon City Health Office, through the Community Health Center, conduct similar socialization events for parents through posyandu cadres to promote the use of HT in reducing post-immunization pain and anxiety in children.
Efektivitas Minyak Atsiri Daun Cengkeh (Syzygium aromaticum) dan Biji Pala (Myristica fragrans) terhadap Low Back Pain: Studi Perbandingan di Kota Ambon Lameky, Vernando Yanry; Akollo, Isak Roberth; Tiwery, Rosdiana Tisye
Jurnal Keperawatan Florence Nightingale Vol 7 No 2 (2024)
Publisher : Sekolah Tinggi Ilmu Kesehatan Stella Maris Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52774/jkfn.v7i2.267

Abstract

Low back pain is a global health problem that affects quality of life and work productivity. The causes vary, from injuries to degenerative diseases. One alternative approach to relieve LBP is clove leaves and nutmeg. Clove leaves contain eugenol, which has anti-inflammatory, analgesic, and muscle relaxant properties, while nutmeg contains myristicin and essential oils that effectively relieve pain. The combination of the two, which can be used in the form of massage oil or herbal ointment, has the potential to provide a synergistic effect in reducing pain and inflammation. This study aims to analyze the effectiveness of clove leaf and nutmeg essential oils in reducing LBP pain in Ambon City. The research method used was quasi-experimental with a pretest-posttest design involving 50 respondents who experienced LBP and were divided into two groups. Data were collected using a numeric pain assessment scale and analyzed using the Paired Sample T-Test. The results showed that 64% of respondents in the clove leaf oil group and 76% in the nutmeg seed oil group experienced moderate pain before the intervention. After the intervention, a significant decrease in pain was found, where the clove leaf group showed an average decrease in pain from 2.36 to 0.24 (p = 0.000), while the nutmeg group from 2.24 to 1.48 (p = 0.001). This study indicates that clove leaf essential oil is more effective in relieving LBP pain than nutmeg seed oil. These findings provide recommendations for using local natural resources as an alternative non-pharmacological therapy in managing LBP.
Co-Authors Akollo, Alberth Djarami, Jayanti Kolibonso, Billy Franscois Lameky, Vernando Yanry Maitimu, Anatji Naomi Oci Tasijawa Pattinasarany, Devan Hendrik Patty, Joanna Cristy Siahaya, Griennasty Sillehu, Sahrir Sitti Rahmah Umniyati Syulce Luselya Tubalawony Tandi, Dan Tiwery, Rosdiana Tisye Tomasila, Boy Tri Baskoro Satoto Tuhuleruw, Bredlcy Fransiscus