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All Journal Buletin Veteriner Udayana
I Ketut Suatha
Laboratorium Anatomi dan Embriologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman Denpasar, Bali, Indonesia
Immunology & microbiology Public Health Veterinary

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EVALUATION OF PRESERVED ORGANS OF PLASTINATION WITH THE DEHYDRATION PHASE AT ROOM TEMPERATURE Ni Nyoman Werdi Susari; I Nengah Wandia; I Ketut Suatha; Luh Gde Sri Surya Heryani
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p26

Abstract

Organ preservation is an effort to maintain the integrity of organs for a long time by preventing further damage and decay processes. Plastination is an organ preservation process by inserting polymer materials to maintain the shape and composition of the organ. This research aims to evaluate the product of a plastination technique carried out using generic chemicals in the community and equipment. The organs used in this plastination process are heart, brain and lungs from the bali cattle. The research materials and tools needed are distilled water, formalin, acetone, liquid silicone rubber, silicon catalyst, 10 ml syringe, plastic bag/tub with lid, vacuum chamber, plastic bucket/tub without lid, and hardening room/box. The vacuum chamber is made from a modified drum so that it can accommodate cadaver plastination (large size). The plastination technique is carried out in four main stages, one of which is the dehydration phase with acetone which is carried out at room temperature. The plastinated organs that are the product of this technique will be evaluated for their flexibility, color, and odor. The results of this research produce plastinated organs that have a stiff texture, pale color, and a non-pungent odor. From the research results obtained, it can be suggested that it needs to be stored in a vacuum for longer (for 1 week) so that the texture can resemble its original shape.  
HISTOMORPHOMETRI OF WHITE BLOOD CELLS OF BALI DUCKS USING HISTOCHEMICAL METHODS I Gusti Ngurah Jagad Anom Ksatriya; Ni Ketut Suwiti; Anak Agung Sagung Kendran; Ni Luh Eka Setiasih; I Ketut Suatha; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p25

Abstract

Bali ducks are one of the local poultry breeds whose meat and eggs are usually used. Bali ducks can experience immune disorders, especially their susceptibility to disease. White blood cells can be used as an indicator of the infection in the body, so white blood cell examination is necessary to evaluate livestock health. This study aims to determine the histomorphometric structure and differences in white blood cells in male and female Bali ducks. This research used blood samples from 8 male Balinese ducks and 8 female Balinese ducks aged two to three months from farms in Mengwi District, Badung Regency. Staining of blood smear was carried out using eosin and methylene blue staining (MDT IndoReagen®). Examination and measurement of blood cell preparations were carried out using an Olympus CX33 microscope and EPView application. Data analysis was carried out using independent samples T-test with the help of SPSS software. The results of histomorphometric examination showed that the heterophyll diameter of male Bali ducks was 5.38±0.62 µm, and the female Bali ducks was 5.23±0.60 µm. The eosinophil diameter of male Bali ducks was 5.49±0.62 µm, and the female Bali ducks was 4.99±0.54 µm. The basophil diameter of male Bali duck was 3.82±0.35 µm, and the female Bali duck was 4.33±0.52 µm. The monocyte diameter of male Bali duck was 5.13±0.72 µm, and the female Bali duck was 4.99±0.37 µm. The lymphocytes diameter of male Bali duck was 4.18±0.74 µm, and the female Bali duck was 4.52±0.58 µm. Based on the research results, it can be concluded that there is no histomorphometric difference between the white blood cells of male and female Bali ducks (P>0.05). Further research is needed regarding the histomorphometric comparison of white blood cells in Bali ducks at different ages to obtain more complete data.
HISTOMORPHOMETRY OF EOSINOPHILS AND ERYTHROCYTES OF CONVENTIONALLY REARED BALI CATTLE Titi Humairah Bahtiar; Ni Ketut Suwiti; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p27

Abstract

Blood tissue is an indicator of the health status of Balinese cattle. The presence of red blood cells or erythrocytes and white blood cells such as eosinophils is very important because they play a role in the infection process. Therefore, this study aims to determine the histology and morphometry of eosinophils and erythrocytes as well as the number of eosinophils in Balinese cattle raised in a conventional way. The samples used were 30 cows, taken from Balinese cows raised in Bulian Village, Kubutambahan District, Buleleng Regency. The blood tissue was prepared with MDT staining. Morphometric measurement of eosinophil and erythrocyte white blood cells using EP View application with 1000x magnification. Counting the number of eosinophils was done per 100 leukocyte cells. The results showed that eosinophils were round in shape and had a purplish-colored 2-lobed nucleus and bright pink granulated cytoplasm with an average diameter of 5.90 ± 1.04 µm. Erythrocytes have a discocyte shape without a purple nucleus and several variations of elliptocyte shapes with a mean diameter of 3.62 ± 0.19 µm. The mean white blood cell eosinophils of conventionally reared Balinese cattle was 7%. Further research is needed to assess health status by looking at other indicators such as physiological status in conventionally reared Balinese cattle.
HISTOMORPHOMETRY OF THE SUPERFICIAL PECTORALIS MUSCULAR AND CRANIAL TIBIALIS MUSCULAR OF BALI DUCKS IN THE GROWTH PHASE I Kadek Ari Satria Prayoga; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p11

Abstract

Superficial pectoralis muscle is a chest muscle that is located on the surface and functions in wing movement. Tibialis cranialis muscle is the top muscle in the calf muscle structure, which functions to support the bird's body. This study aims to determine the histomorphometry of the superficial pectoralis muscle and cranial tibial muscle of male and female Bali ducks in the growing phase. This research used 20 Balinese ducks aged 12 weeks. Direct anatomical examination and histological structure with a binocular light microscope. Histomorphometry was measured using the Olympus Cellsens Standard application. Anatomy and histology results were analyzed using descriptive qualitative analysis, and histomorphometry using the ANOVA test with mean estimation. Histological structure of the superficial pectoralis muscle and cranial tibial muscle consists of muscle fibers, fasciculus, endomysium, perimysium and epimysium connective tissue. Histomorphometry of fascicle size, perimysium connective tissue thickness, and superficial pectoralis muscle endomysium were significantly different (P<0.05). Histomorphometry of the size of the fasciculus, perimysium connective tissue and endomysium of the cranial tibial muscle was not significantly different (P>0.05) in different genders. It can be concluded that the superficial pectoralis muscle and cranial tibial muscle of males and females in the growing phase are the same in terms of anatomical structure, but the size of the histological structure is different. Histomorphometry of the superficial pectoralis muscle of male and female Bali ducks is significantly different (P<0.05), but not for the tibialis cranialis muscle. Further research is needed regarding the muscles of Bali ducks at other ages.
STRUCTURE AND MORPHOMETRY OF BALI DUCK PANCREAS IN THE GROWER PHASE I Gde Andhika Putra Pratama; Ni Luh Eka Setiasih; Sri Kayati Widyastuti; Ni Ketut Suwiti; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p12

Abstract

The pancreas is part of the digestive system which acts as a digestive aid organ. The pancreas has two functions, namely exocrine and endocrine. This study aims to determine the structure and morphometry of the pancreas of Bali ducks (Anas Sp) of different sexes in the growing phase. This research used 18 Balinese ducks which were divided into 2 groups, namely male and female with 9 each (3 months old). The method for examining anatomical structures is carried out by direct observation and histological structures using a binocular light microscope. Morphometry was measured using calipers for length and width, scales for pancreatic weight, and measuring cups for volume. Histomorphometry was measured using the Olympus cellSens Standard application. The results of the anatomical and histological structure data were analyzed using qualitative descriptive analysis, while the morphometric data used the Independent Sample T-Test. The anatomical structure of the Bali duck pancreas is flat like a lobed tongue and pink in colour, consisting of a capsule, islets of Langerhans, acini, intercalary ducts, intralobular ducts, interlobular ducts, intralobular septa, interlobular septa, veins and arteries. The results of morphometric measurements of Bali duck pancreas showed significant differences (P<0.05) in the weight and volume of the pancreas. Histomorphometric measurements of the area of the islets of Langerhans, the area of the acini, the thickness of the intralobular septa, and the thickness of the interlobular septa showed that they were not significantly different (P>0.05) in both sexes. The anatomical structure and histology of the pancreas of male and female Bali ducks are the same, but there are differences in morphometry
HISTOMORPHOMETRY OF THE DEXTER VENTRICLE OF LOCAL BALI DUCKS AT GROWTH PHASE Delima Marsinta Ida Pasaribu; Ni Luh Eka Setiasih; Luh Gde Sri Surya Heryani; I Ketut Berata; Ni Nyoman Werdi Susari; I Ketut Suatha
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p20

Abstract

Heart is the main organ in blood circulation which plays a role in pumping blood to all body cells and returning to the heart. Avian heart has four chambers consisting of two atria (dexter and sinister) and two ventricles (dexter and sinister), each chamber has three layers of walls consisting of epicardium, myocardium and endocardium. This study aimed to obtain information about the histological structure and histomorphometry of ventriculus dexter of the bali duck’s heart at grower phase. The study sample used 18 bali ducks, which were divided into two gender groups and each consisted of 9 ducks (3 months old). Ventriculus dexter sample was the processed into a histology preparation by Haematoxylin-Eosin (HE) staining. Anatomy and histology results were analyzed using descriptive qualitative analysis, while histomorphometry used the anova test with mean estimation using the SPSS 26 program. The mean thickness of the epicardium, myocardium, and endocardium in males respectively were 43,11 ± 12,05µm, 1.145,19 ± 133,99µm, 23,41 ± 6,91µm. While in females respectively were 34,98 ± 17,03µm, 1.367,41 ± 412,43µm, 11,86 ± 5,06µm. Histomorphometric data that has been analyzed shows that the results of measuring the thickness of the endocardium are significantly different (P<0,05), while measuring the thickness of the epicardium and myocardium was not significantly different (P>0,05).. It can be concluded that the anatomical structure of the heart and the histological structure of the ventriculus dexter of bali ducks didn’t differ between treatment males and females, but there were differences in the histomorphometry of the endocardial layer. Research on the heart of balinese ducks is still rarely carried out, so further research is needed on the structure and histomorphometry of the epicardium layer, myocardium layer, and endocardium layer of the dexter ventricle or other heart chambers in bali ducks at different phases.
Co-Authors Anak Agung Sagung Kendran Delima Marsinta Ida Pasaribu I Gde Andhika Putra Pratama I Gusti Ngurah Jagad Anom Ksatriya I Kadek Ari Satria Prayoga I Ketut Berata I Nengah Wandia Linus Putra Jaya Lase Luh Eka Setiasih Luh Gde Sri Surya Heryani Ni Ketut Suwiti Ni Ketut Suwiti Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Sri Kayati Widyastuti Titi Humairah Bahtiar