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All Journal Indonesian Journal of Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) 3BIO: Journal of Biological Science, Technology and Management Narra J BIODIVERS - BIOTROP Scientific Magazine
Neil Priharto
School Of Life Sciences And Technology, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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OPTIMIZATION OF Bacillus paramycoides FERMENTATION MEDIUM TO INCREASE THE PRODUCTION OF 5-AMINOLEVULINIC ACID IN A 10 LITER FERMENTER Dicky Adihayyu Monconegoro; Dea Indriani Astuti; Neil Priharto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (705.166 KB) | DOI: 10.29122/jbbi.v9i2.5351

Abstract

ABSTRACT 5-Aminolevulinic acid is an essential precursor for the biosynthesis of tetrapyrrole compounds, such as chlorophyll and heme. 5-ALA has the potential to be used as a plant growth and antioxidant activity enhancer. 5-ALA can be produced through fermentation by Bacillus paramycoides. This study aimed to optimize B. paramycoides fermentation medium to increase 5-ALA production. The optimization was carried out using response surface method (RSM) experimental design. 5-ALA production in a 10 L fermenter was conducted using an optimized medium and supplemented with MSG as a precursor and wood vinegar as an inhibitor. The results showed that the best medium composition was 27.78 g L-1 molasses; 9.145 g L-1 urea; 8.838 g L-1 NaCl; and 32.07 g L-1 glucose, resulting in 10.749 (log CFU mL-1) and 255.30 µM 5-ALA. 5-ALA production trial in a 10 L fermenter produced 581.82 µM 5-ALA. Medium optimization and precursor-inhibitors addition in the fermentation increased the 5-ALA yield 3.2 times compared to before optimization.   ABSTRAK 5-Aminolevulinic acid (5-ALA) merupakan prekursor penting dalam pembentukan senyawa tetrapirol seperti klorofil dan heme. 5-ALA memiliki potensi sebagai senyawa pemacu pertumbuhan dan peningkatan kandungan antioksidan tanaman. 5-ALA dapat dihasilkan melalui fermentasi oleh Bacillus paramycoides. Tujuan dari penelitian ini adalah melakukan optimasi media fermentasi B. paramycoides untuk meningkatkan konsentrasi 5-ALA. Optimasi media fermentasi dilakukan dengan menggunakan desain eksperimen response surface method (RSM). Setelah itu, dilakukan uji coba produksi 5-ALA pada fermentor 10 L menggunakan media hasil optimasi RSM dan suplementasi prekursor MSG dan inhibitor asap cair. Hasil optimasi media RSM menunjukkan bahwa komposisi media terbaik adalah 27,78 g L-1 molase; 9,145 g L-1 urea; 8,838 g L-1 NaCl; dan 32,07 g L-1 glukosa dengan jumlah sel (log CFU mL-1) sebesar 10,749 dan konsentrasi 5-ALA sebesar 255,30 µM. Uji coba produksi 5-ALA pada fermentor 10 L menghasilkan konsentrasi 5-ALA sebesar 581,82 µM. Optimasi media dan penambahan prekursor-inhibitor pada fermentasi dapat meningkatkan produksi 5-ALA sebanyak 3,2 kali lipat dibandingkan sebelum optimasi.
Cultivation and Propagation Techniques of Trichoderma harzianum Aluna Gita Arumsari; Alfidhiya Amany Ramli; Neil Priharto; Sunardi Ikay; Risa Rosita
BIODIVERS - BIOTROP Science Magazine Vol. 2 No. 2 (2023): BIODIVERS (BIOTROP Science Magazine) - Biodiversity for All
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56060/bdv.2023.2.2.2098

Abstract

This research was conducted to determine the effect of temperature on the growth of Trichoderma harzianum. Trichoderma harzianum has functions to prevent the growth of diseases in plants, increase plant growth, and decompose organic matter. The research was conducted at the Phytopathology and Biosystem and Landscape Management Laboratory at SEAMEO BIOTROP, Bogor. The study was analyzed using a Completely Randomized Design (CRD) with different temperature treatments with five replications. The parameter observed was the diameter of the fungus growth after 6 days incubation period. Based on the research results, T. harzianum grew well when the fungus was incubated at 23 °C compared to 40 °C.
Enhancing Lipid Extraction from Chlorella vulgaris Microalgae for Biodiesel Production: Application of Natural Deep Eutectic Solvent (NaDES) in Cell Disruption Priharto, Neil; Nadia Yasmin Dicky
3BIO: Journal of Biological Science, Technology and Management Vol. 6 No. 2 (2024)
Publisher : School of Life Sciences and Technology, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/3bio.2024.6.2.2

Abstract

Natural deep eutectic solvent (NaDES) pre-treatment offers a promising alternative strategy to enhance lipid extraction efficiency from microalgae by influencing the integrity of the cell wall, thereby improving solvent accessibility to the cytoplasm and facilitating the release of intracellular lipid content. In this study, Chlorella vulgaris biomass underwent pre-treatment with four different NaDES formulations, each based on choline chloride (ChCl) with varying molar ratios: ChCl : Glycerol (1:2), ChCl : Glycerol (1:1), ChCl : Oxalic Acid (1:2), dan ChCl : Oxalic Acid (1:1).We analyzed the impact of these NaDES pre-treatments on lipid yield and fatty acid profiles. The extracted lipids exhibited an acid value of 42.56 mg KOH/g fat and a free fatty acid content of 0.25%. Samples subjected to NaDES treatment showed significant increases in lipid extraction efficiency, with lipid yields ranging from 1.25 to 2.3 times higher than those of untreated biomass extracted using hexane (p < 0.05). The highest lipid yield was observed in samples treated with ChCl : Glycerol (1:1), achieving a total lipid yield of 19.44% (w/w), more than double that of the untreated biomass. Although minor variations in fatty acid profiles were noted due to the NaDES treatment, the dominant fatty acids in each variation remained palmitic acid (C16:0, 21-29.5%) and oleic acid (C18:1, 13-43.88%).
Optimized bioethanol production from banana stem waste via simultaneous saccharification and fermentation with Saccharomyces cerevisiae Neil Priharto; Agung Setiawan; Dea Indriani Astuti
Indonesian Journal of Biotechnology Vol 30, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.98638

Abstract

Indonesia, one of the world’s largest banana producers, generates significant quantities of banana stem waste, leading to environmental challenges. This study explores the potential of converting this lignocellulosic biomass into bioethanol using a combination of steam pretreatment and simultaneous saccharification and fermentation (SSF) with Saccharomyces cerevisiae. The SSF process integrates enzymatic hydrolysis and fermentation, streamlining bioethanol production. The research applied the Taguchi method with an L9(34) orthogonal array to optimize key parameters, including enzyme concentration, particle size, temperature, and pH. Optimal conditions—5% enzyme concentration (v/v), 60 mesh banana powder, 35 °C and pH 5.00—yielded a maximum ethanol concentration of 9 g/L. Enzyme concentration and particle size were identified as critical factors in enhancing bioethanol yield. This study highlights the potential of banana stem waste as a sustainable resource for bioethanol production, contributing to waste reduction and renewable energy development.
Development of an inactivated viral transport medium for diagnostic testing in low-resource countries Rahmani, Silmi; Meitha, Karlia; Septiani, Popi; Priharto, Neil; Kamarisima, Kamarisima; Ningrum, Ratih A.; Angelina, Marissa; Agustiyanti, Dian F.; Wisnuwardhani, Popi H.; Nugroho, Herjuno A.; Tan, Marselina I.
Narra J Vol. 5 No. 3 (2025): December 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i3.2068

Abstract

Viral transport medium (VTM) is crucial for retaining clinical specimens, such as the virus or its genetic material from the mucus of respiratory tract of coronavirus disease 2019 (COVID-19) suspected patients. However, the locally produced VTM in Indonesia lacks the ability to inactivate the virus, risking the safety of diagnostic personnel. The aim of this study was to formulate inactive VTM (iVTM) incorporating chaotropic agents like guanidine salt, along with anionic detergents, chelators, buffers, and surfactants, to inactivate the virus while maintaining RNA integrity. Viral RNA stability in iVTM (pH 4 and pH 6) was evaluated for 30 days at 4°C and 25–28°C. In vitro inactivation test was performed on SARS-CoV-2 isolate (variant B1). The stability test revealed that storing the clinical specimens in iVTM at pH 6 maintained severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) detectability by qPCR for up to 30 days at cold and room temperatures. Stability assessments conducted over a 4-month period (at 25–28°C) on iVTM with a pH of 6 revealed clear appearance, consistent pH stability, no alteration in the solution color, and no indications of bacterial or fungal contamination. Results from an in vitro inactivation assay demonstrated that iVTM pH 6 eliminated SARS-CoV-2 infectivity within just five minutes of contact. These findings suggest that iVTM pH 6 offers a safer and cost-effective alternative for handling and transportation of clinical specimens.
Development of an inactivated viral transport medium for diagnostic testing in low-resource countries Rahmani, Silmi; Meitha, Karlia; Septiani, Popi; Priharto, Neil; Kamarisima, Kamarisima; Ningrum, Ratih A.; Angelina, Marissa; Agustiyanti, Dian F.; Wisnuwardhani, Popi H.; Nugroho, Herjuno A.; Tan, Marselina I.
Narra J Vol. 5 No. 3 (2025): December 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i3.2068

Abstract

Viral transport medium (VTM) is crucial for retaining clinical specimens, such as the virus or its genetic material from the mucus of respiratory tract of coronavirus disease 2019 (COVID-19) suspected patients. However, the locally produced VTM in Indonesia lacks the ability to inactivate the virus, risking the safety of diagnostic personnel. The aim of this study was to formulate inactive VTM (iVTM) incorporating chaotropic agents like guanidine salt, along with anionic detergents, chelators, buffers, and surfactants, to inactivate the virus while maintaining RNA integrity. Viral RNA stability in iVTM (pH 4 and pH 6) was evaluated for 30 days at 4°C and 25–28°C. In vitro inactivation test was performed on SARS-CoV-2 isolate (variant B1). The stability test revealed that storing the clinical specimens in iVTM at pH 6 maintained severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) detectability by qPCR for up to 30 days at cold and room temperatures. Stability assessments conducted over a 4-month period (at 25–28°C) on iVTM with a pH of 6 revealed clear appearance, consistent pH stability, no alteration in the solution color, and no indications of bacterial or fungal contamination. Results from an in vitro inactivation assay demonstrated that iVTM pH 6 eliminated SARS-CoV-2 infectivity within just five minutes of contact. These findings suggest that iVTM pH 6 offers a safer and cost-effective alternative for handling and transportation of clinical specimens.
Co-Authors Agung Setiawan Agustiyanti, Dian F. Alfidhiya Amany Ramli Aluna Gita Arumsari Dea Indriani Astuti Dea Indriani Astuti Dicky Adihayyu Monconegoro Kamarisima, Kamarisima Marissa Angelina Meitha, Karlia Nadia Yasmin Dicky Ningrum, Ratih A. Nugroho, Herjuno A. RAHMANI, SILMI Risa Rosita Septiani, Popi Sunardi Ikay Tan, Marselina I. Wisnuwardhani, Popi H.