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All Journal Jurnal Media Analis Kesehatan Journal of Nursing and Health (JNH) Akper Yakpermas Banyumas
Amellya Octifani
Universitas Anwar Medika
Health Professions Immunology & microbiology Nursing Public Health

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EFEK MULTI STRAIN PROBIOTIK TERHADAP KADAR LDL-C Rattus norvegicus HIPERLIPIDEMIA Kadeq Novita Prajawanti; Jusak Nugraha; Harianto Notopuro; Amellya Octifani; Yohanes Adrian Kapri Negara
Jurnal Media Analis Kesehatan Vol 14, No 1 (2023): JURNAL MEDIA ANALIS KESEHATAN
Publisher : Poltekkes Kemenkes Pakassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mak.v14i1.3256

Abstract

Hyperlipidemia is an increase in one or more plasma lipids and the main cause is related to the pattern of food intake. Among the many intestinal microbes, Lactobacillus, Bifidobacterium have been shown to affect the host beneficially by improving the intestinal microbial balance and are therefore categorized as probiotics. The aim of this study was to determine the effect of multi-strain probiotics Lactobacillus rhamnosus and Bifidobacterium bifidum on reducing LDL-C in rat fed HFD 1% per body weight. This True Experimental Research with Pretest-Posttest Design used Rattus norvegicus which was divided into 5 groups each K- (standard feed + tap water), K+ (HFD without probiotics), P1 (HFD + 2mL probiotics), P2 (HFD + 2 .5mL probiotics) and P3 (HFD + 3mL probiotics). The results of the LSD test for LDL cholesterol levels after administration of probiotics, the group that had a significantly different value was in the K- to K+ group with p=0.00; K+ to P1, P2 and P3 with p 0.00 each. Meanwhile, among the treatment groups that were given probiotics, only the P2 to P3 groups showed a significant difference with a p value = 0.037.
IDENTIFIKASI GEN MULTIPLEX DNA PADA BAKTERI PENGHASIL ESBL DAN MRSA DARI ISOLAT ULKUS PASIEN DM AMELLYA OCTIFANI
Journal of Nursing and Health Vol. 10 No. 1 (2025): Journal of Nursing and Health
Publisher : Yakpermas Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52488/jnh.v10i1.434

Abstract

Elevated blood glucose levels in people with DM are prone to complications and can create a favourable environment for bacterial growth. Prolonged use of antibiotics leads to the possibility of resistance and it is necessary to identify resistance genes as soon as possible to reduce the prevalence of the spread of bacteria that infect diabetic wounds. The type of research used is observational with quantitative description. For the identification of DNA multiplex genes in ESBL-producing bacteria and MRSA from ulcer isolates of DM patients. The results of the study obtained 16 isolates from outpatients and inpatients as many as 63% of isolates identified s.aureus bacteria and e.coli 37%. DNA multiplex results of s.aureus bacterial isolates detected the mecA gene in 32% and the ctxm gene in 6%. In e.coli bacterial isolates, 6% ctx-m gene and 18% mecA gene were detected. Multiplex PCR method can be used to identify mec-A and ctx-m genes as coding genes of MRSA and ESBL producing bacteria in S.aureus and E.coli isolates from diabetic ulcer swabs..
Co-Authors Harianto Notopuro Jusak Nugraha Kadeq Novita Prajawanti Yohanes Adrian Kapri Negara