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All Journal Jurnal Teknologi Laboratorium Jurnal Kesehatan Komunitas JURNAL FARMASIMED (JFM) Jurnal Pengmas Kestra (JPK) Jurnal Laboratorium Khatulistiwa Medistra Medical Journal (MMJ)
Saadah Siregar, Saadah
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Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Other

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Efektivitas Variasi Garam Salmiak (NH4Cl) Dan Sentrifugasi Pada Pemeriksaan Basil Tahan Asam Penderita Tuberculosis Siregar, Saadah; Supriatin, Yati; Noor, Lanny
Jurnal Teknologi Laboratorium Vol 6 No 2 (2017): 2017 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.857 KB) | DOI: 10.29238/teknolabjournal.v6i2.91

Abstract

According to a report by WHO Indonesia, it is a case number three infected with tuberculosis. Tuberculosis disease is caused by Mycobacterium tuberculosis that belongs to acid-fast bacilli bacteria (BTA). Sputum smear examination microscopically still has many deficiencies caused by mucoprotein in sputum so that BTA cannot be separated from sputum, this is causing a false negative in reading result Microscope. The objective of this research is to know the most effective variation of concentration, speed and time of centrifugation on smear examination in tuberculosis patients. The research method used is experimental method and Design of complete Random Block design. Data were analyzed by statistic test with ANOVA test on SPSS. The result of the research is that at concentration 2% NH4Cl It can increase the number of BTA with optimal centrifugation speed / effective at 5000 rpm for 15 minutes. Suggestion for further research is to use salmaniac salt concentration with a concentration lower than 2%.
Pengaruh Keberadaan Jentik dan Perilaku 3M Plus terhadap Kejadian Demam Berdarah Dengue Siregar, Saadah; Mulyani, Sri; Rizky, Vincentia Ade; Akmal, Dzul; Sutriyawan, Agung
Jurnal Kesehatan Komunitas Vol 9 No 3 (2023): Jurnal Kesehatan Komunitas
Publisher : LPPM Hang Tuah Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25311/keskom.Vol9.Iss3.1392

Abstract

An infectious disease that is still a public health problem today is dengue hemorrhagic fever. Prevention efforts that can be done are by breaking the chain of transmission by eradicating mosquito nests. The purpose of this study is to analyze the influence of the presence of larvae and the behavior of 3M plus on the incidence of dengue hemorrhagic fever. The study used a cross-sectional design. The research was carried out in the working area of Puskesmas Puter, in July - August 2022. The population in this study was 7,110 heads of households. The number of samples was 67 heads of families, taken using stratified random sampling techniques. The statistical test used is the chi-square test. The results showed that the variables that affect the incidence of dengue hemorrhagic fever are the presence of larvae (p = 0.006 and POR = 4.8), closing water reservoirs (p = 0.009 and POR = 4.8), draining water reservoirs (p = 0.041 and POR = 3.5), and larvicides (p = 0.027 and POR = 4.4). This research can conclude that the incidence of DHF can be influenced by several factors, namely the presence of mosquito larvae, and the behavior of closing, draining, and sprinkling larvicide powder. It is recommended that health workers further optimize the role of levels in monitoring mosquito nest eradication behavior carried out by the community.
SOCIALIZATION OF GIVING GINGER TO REDUCING PATHOGENIC AND FOOD-DESTROYING BACTERIA IN CHICKEN MEAT: SOCIALIZATION OF GIVING GINGER TO REDUCING PATHOGENIC AND FOOD-DESTROYING BACTERIA IN CHICKEN MEAT Rizky, Vincentia Ade; Siregar, Saadah; Rahayu, Asvia; Krisdianilo, Visensius
JURNAL PENGMAS KESTRA (JPK) Vol. 2 No. 2 (2022): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.259 KB) | DOI: 10.35451/jpk.v2i2.1432

Abstract

Broiler chicken meat is a food source of animal origin that is widely consumed in Indonesia. The affordable price makes the demand for broiler chicken meat increasing. Chicken meat is easily contaminated by various microorganisms from the surrounding environment. Several types of microbes that can contaminate meat include E. Coli bacteria, Clostridium botulinum bacteria, Clostridium Perfringens, Salmonella bacteria, Staphylococcus aureus, and Anthrax. Microbial contamination in the form of Salmonella bacteria is generally found in broiler chicken meat. To overcome microbial contamination, the researchers conducted research on the effect of ginger on reducing the number of bacteria in chicken meat. This socialization aims to provide information to the public to be able to use ginger as an inhibitor and reduce the number of pathogenic bacteria and food destroyers in chicken meat. The results of this socialization were obtained from previous research which concluded that a decrease in the number of bacteria was due to the administration of ginger. This is because ginger contains secondary compounds such as phenols, treponoids and benzaldehyde which are bacteriostatic so that they function to inhibit the growth of microorganisms. The results of the socialization carried out in the community around Lubuk Pakam stated that they understood the processing and storage techniques (100%).
EFEKTIFITAS PENGGUNAAN PEWARNA ALTERNATIF PREPARAT PERMANEN TELUR NEMATODA USUS MENGGUNAKAN PEWARNA RHODAMIN B Siregar, Saadah; Krisdianilo, Visensius; Rizky, Vincentia Ade
Jurnal FARMASIMED (JFM) Vol 2 No 1 (2019): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.612 KB) | DOI: 10.35451/jfm.v2i1.194

Abstract

Staining is one of support microscopic examination in the identification of worm eggs. Staining techniques are needed to clarify the various elements and the description of the microscopic worm eggs and distinguish it from the surrounding dirt. Hematoxylin and Eosin staining method that is widely used in the staining of histological tissue. Dyes commonly used in the examination of intestinal nematode eggs on is dye eosin. To added reference dye Rhodamine B dye used as an alternative to dye eosin. The method used is experiment by conducting concentration ratio of Rhodamine B 1%, 1.5%, 2% and 2.5% to 2% eosin control. The results based on Kruskal-Wallis Test showed the normality test is 0.357, p-value sig (0.357) > 0.05, so that the data group to be tested otherwise normal. Then performed statistical tests Kruskal WallisTtest to determine the accuracy of the file thus obtained p-value (0.407) > 0.05, meaning there is no significant difference in the concentration of the dye Rhodamine B of the entire treatment. Morphology of worm eggs clearly visible from the outside layer to the inside layer, the visual field Rhodamine B dye is clearly visible, color contrast eggs are also clearly visible. From the research conducted, it can be concluded that the dye Rhodamine B dye can be used as an alternative to dye eosin.
PERBANDINGAN AKTIVITAS ANTIBAKTERI INFUSA DAUN JERUK NIPIS (CITRUS AURANTIFOLIA) DAN DAUN JERUK PURUT (CITRUS HYSTRIX) TERHADAP BAKTERI ESCHERICHIA COLI Siregar, Saadah; Indriani, Indriani; Vincentia Ade Rizky, Vincentia; Visensius Krisdianilo, Visensius; Anna Teresia Marbun, Romauli
Jurnal FARMASIMED (JFM) Vol 3 No 1 (2020): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i1.524

Abstract

Plant Lime (Citrus aurantifolia) and Plant Kaffir Lime (Citrus hystrix) including Rutacea family. Part of the plant lemon and lime are used as a drug other than fruit and leaves that can be used as medicine. This study aims to determine the antibacterial activity infuse lime leaves and lime leaves. Examination of the characteristics simplicia macroscopic examination. Phytochemical screening of compounds includes examining alkaloids, flavonoids, tannins and infuse saponin. Ekstrak conducted using distilled water solvent. Test of antibacterial activity against Escherichia coli bacteria carried by the agar diffusion method using a paper disc. Results obtained from macroscopic examination for lime leaves are fresh leaves leaf-shaped single smooth surface and the lower surface of the leaves are light green, the dark green upper surface. If torn, lime leaves produce coarse fibers. The leaves are small with a width of 3-5 cm. For lime leaves are fresh leaves, leaf litter pinnate compound leaves one. Child leaf blade oval to oblong, base rounded or blunt, the blunt end up pointed, 8-15 cm long, 2-6 cm wide, the upper surface colour some what shiny dark green, light green below the surface. Results of phytochemical screening simplicia powder lime leaves and lime leaves that contain a class of alkaloids, flavonoids and tannins. Antibacterial activity test results showed that there were differences between the antibacterial infusion lime leaves and lime leaves kaffir lime leaves which are more effective against the bacteria Escherichia coli compared kaffir lime leaves.
UJI EFEKTIFITAS DAUN PANDAN WANGI (PANDANUS AMARYLLIFOIUM ROXB) SEBAGAI ANTIJAMUR TERHADAP PITYSPORUM OVALE siregar, Saadah; Topia, Riana
Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i2.591

Abstract

The Pandan Wangi leaf plant is a plant that belongs to the Pandanaceae family. The metabolite content of fragrant pandan leaves (Pandanus amaryllifolius Roxb.) Alkaloids, flavonoids, saponins, tannins are known to have an antifungal effect. Pityrosporum ovale is a yeast or single-celled fungus that belongs to the genus Malassezia sp, and belongs to the Cryptococcaceae family. Pityrosporum ovale causes superficial dermatomycosis which affects the stratum corneum in the epidermis layer. This fungus is a normal flora on the scalp, but in conditions of hair with excess oil glands, this fungus can thrive. The purpose of this study was to determine the inhibition zone of fragrant pandanus leaves (Pandanus amaryllifolius Roxb.) Concentrations of 10%, 20%, 30%, and 40% of Pytirosporum ovale on Potato Dextrose Agar media by diffusion method using disc paper. The diffusion method was used to measure the diameter of the zone of inhibition against fungal growth. The positive control used was ketoconazole 2% and negative control DMSO 1%. The results of the study using the diameter of the inhibition zone at a concentration of 10% were 7.86 mm, the inhibition zone at a concentration of 20% was 8.53 mm, the inhibition zone at a concentration of 30% was 8.76 mm, and the inhibition zone at a concentration of 40% was 9, 43 mm.
PERBEDAAN MODIFIKASI LARUTAN ZnS (Zinc sulfite) DENGAN NaCl (Natrium Cloride) 0.9 % METODE FLOTASI TERHADAP JUMLAH TELUR CACING Soil Transmitted Helminth (STH) Siregar, Saadah; Manurung, Arnita Elisabet; Rizky, Vincentia Ade; Krisdianilo, Vinsensius
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1405

Abstract

Worms is a disease caused by the entry of parasites in the form of worms into the human body due to the influence of environmental factors or food that is not kept clean. Factors that cause the risk of helminthiasis in humans include food that is infected with worm eggs, feet that are in direct contact with soil containing worm vectors because they do not wear footwear, the habit of defecating in random places, availability of clean water, hand washing, washing habits. plates, And nail hygiene. The purpose of this study was to determine the difference in the number of worm eggs to be found using ZnS 1% and NaCl 0.9% solution using the flotation method. The data obtained from faecal examination using the flotation method with ZnS 1% and NaCl 0.9%, namely Soil Transmitted Helminth (STH) worm eggs, then the number of eggs will be presented in table form and analyzed descriptively. Result With the average yield of worm eggs found in  a solution of ZnS  1% and NaCl 0.9% using the flotation method for the number of Soil Transmitted Helminth (STH) worm eggs. In ZnS 1% solution the average is 2.12 with 4 Ascaris lumbricoides eggs, Trichuris trichura 2 and 3 hookworms while in 0.9% NaCl solution the average is 1.31 with 5 Ascaris lumbricoides worm eggs, Trichuris trichura 1 and worms mine 1. The Manny Whitney statistical test showed results of Sig 0.003 (P<0.005), so it was concluded that there was a difference in the number of STH worm eggs found using ZnS 1% solution and 0.9% NaCl using the flotation method.
Pewarnaan alternatif alami daun miana (Coleus scutellarioides (L) benth) sebagai pengganti gentian violet pada pewarnaan gram bakteri staphylococcus aureus RIZKY, VINCENTIA ADE; SIREGAR, SAADAH; KRISDIANILO, VISENSIUS; RISKA WAHYUNI
Medistra Medical Journal (MMJ) Vol 1 No 2 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v1i2.2095

Abstract

Daun miana (Coleus scutellarioides (L) benth) yang memiliki corak ungu kemerahan mengindifikasikan terdapat antosianin, salah satu variannya yaitu crispa. Penelitian ini bertujuan untuk menguji zat warna antosianin pada sari daun miana sebagai pengganti gentian violet pada pewarnaan gram, Jenis penelitian yang digunakan adalah eksperimental/eksperimen. penelitian ini bertujuan untuk mengetahui apakah sari daun miana (Coleus scutellarioides (L) benth) mampu sebagai bahan alternatif pengganti reagen gentian violet pada bakteri Staphylococcus aureus. Dan pada penelitian ini dilakukan perasan sari daun miana (Coleus scutellarioides (L) benth). sampel bakteri Staphylococcus aureus dibuat sediaan preparat dan pewarnaan menggunakan gentian violet sebagai bahan kontrol dan menggunakan sari daun miana (Coleus scutellarioides (L) benth) sebagai eksperimen menggunakan konsentrasi sari daun miana yaitu 1%, 0,1% dan juga 0,5%. Data yang diperoleh diolah mengggunakan analisa data deskriftif. Hasil penelitian pada sari daun miana sari konsentrasi 1%, dan 0,1% mampu mewarnai bakteri Staphylococcus aureus dibandingan dengan konsentrasi 0,5%. Penelitian ini dapat juga dikembangkan dengan uji coba pada daun lain yang memiliki kandungan antosianin dan perlu juga melalukan konsentrasi yang lebih tinggi atau pun menggunakan metode yang lain.
Utilization of Lemon Juice (Citrus limon L.) as an Alternative Substitute for Sodium Citrate in Blood Sedimentation Rate Examination RIZKY, VINCENTIA ADE; PUTRI, IKKE ELMALIA; SIREGAR, SAADAH
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2370

Abstract

Background: The erythrocyte sedimentation rate (LED) examination is a hematological test that describes the rate at which red blood cells settle in plasma, using 3.8% sodium citrate anticoagulant and is measured in mm/hour. Lemon (Citrus limon L.) is a plant with natural properties as a metal binding agent or chelating agent. Lemons contain vitamin C, citric acid, potassium, and limonene. Citric acid compounds are able to form complex bonds with metals because they have COOH and -OH functional groups. Apart from citric acid, lemons contain flavonoids which function as antiviral, anticancer and antiallergenic. Objective: To test whether lemon juice (Citrus limon L.) can be used as an alternative ingredient to replace sodium citrate in LED tests. Method: This research is an experiment using the Westergreen method. Results: LED examination with 3.8% sodium citrate produced a value of 10 mm/hour. Lemon juice with a concentration of 3.8% produces a value of 15 mm/hour, 10% produces a value of 10 mm/hour, 50% produces a value of 18 mm/hour, and 100% produces a value of 0 mm/hour. Conclusion: A lemon juice concentration of 10% showed results closest to the control, with similar LED values. Thus, lemon juice (Citrus limon L.) can serve as a substitute for sodium citrate in the LED test
Utilization of Kluwih Seed Starch (Artocarpus camansi) as an Alternative Growth Medium for Aspergillus niger Fungus siregar, saadah; DALIMUNTHE, HAFIKA; RIZKY, VINCENTIA ADE
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2372

Abstract

Background: Carbohydrates in kluwih seeds have sufficient nutrients, such as potatoes and tubers, making it possible to be used as a medium for mushroom growth. The nutritional composition of breadfruit contains 25% carbohydrates, 1.5% protein, and 0.3% fat. Aspergillus niger fungi require nutrients such as carbohydrates and protein to grow. This study aims to determine whether kluwih seed starch can be used as an alternative medium for the growth of Aspergillus niger fungi. Method: This study used a pre-experimental design with laboratory observation. The study was conducted by utilizing kluwih seed starch as a medium, compared to Potato Dextrose Agar (PDA) and Sabouraud Dextrose Agar (SDA). Results: The results showed that kluwih seed starch can be used as an alternative medium for the growth of Aspergillus niger, with fungal colonies that grow macroscopically and microscopically almost the same as PDA and SDA media. Conclusion: Kluwih seed starch can be an alternative substitute for PDA media for the growth of Aspergillus niger.
Co-Authors Akmal, Dzul Anna Teresia Marbun, Romauli DALIMUNTHE, HAFIKA Indriani Indriani Krisdianilo, Vinsensius Krisdianilo, Visensius Lanny Noor, Lanny Manurung, Arnita Elisabet PUTRI, IKKE ELMALIA Rahayu, Asvia Riska Wahyuni Sri Mulyani Sutriyawan, Agung Topia, Riana Vincentia Ade Rizky Visensius Krisdianilo, Visensius Yati Supriatin, Yati