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All Journal Livestock and Animal Research Chalaza Journal of Animal Husbandry
Iswati Iswati
Politeknik Pembangunan Pertanian Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Immunology & microbiology Veterinary

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Spermatozoa Quality of Kampung Unggul Balitbangtan (KUB) Chicken at 5oC Storage Supplemented with Glucose in Liquid Semen Diluent J. Junaedi; Nurul Isnaini; Muhammad Halim Natsir; I. Iswati; Trinil Susilawati
Chalaza Journal of Animal Husbandry Vol 8, No 1 (2023): Chalaza Journal of Animal Husbandry
Publisher : UNIVERSITAS SEMBILANBELAS NOVEMBER KOLAKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/chalaza.v8i1.1932

Abstract

This research was conducted to determine the effect of glucose supplementation in liquid semen diluent on the quality of Kampung Unggul Balitbangtan (KUB) spermatozoa.The chicken used is KUB chicken as many as 8 tails.The research procedure consists of; the manufacture of diluent, collection of semen, dilution of semen, storage, and evaluation of semen.Evaluation of semen was carried out on motility, viability, abnormalities, and plasma membrane integrity at 0 and 3 days of storage.Data were analyzed using the T-test to compare the quality of spermatozoa with added glucose into the liquid semen diluent and without the addition of glucose.The addition of glucose into the diluent was able to maintain spermatozoa motility at 35.00 ± 11.50, spermatozoa viability at 41.42 ± 10.15, and spermatozoa plasma membrane integrity at 82.94 ± 7.42 at 5oC storage for three days.This study concluded that glucose supplementation into liquid semen diluent was able to maintain plasma membrane motility, viability, and integrity at 5oC storage for three days, spermatozoa quality of KUB chickens was able to survive at 5oC storage for three days, and glucose can be added to the liquid semen diluent at a dose of 10 mM.
Evaluating sperm quality characteristics obtained through the female teaser method in native chicken breeds Setya Budhi Udrayana; Kasianto Kasianto; Iswati Iswati; Intan Galuh Bintari
Livestock and Animal Research Vol 21, No 3 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i3.66495

Abstract

Objective: The quality and quantity of chicken sperm are critical factors influenced by the semen collection method. Therefore, this study aims to evaluate the sperm quality obtained from three native chicken breeds using the female teaser method.Methods: These three native chicken breeds are Arabic, Birma, and Kampung Unggul Balitbangtan (KUB) chickens. Each breed comprised a group of three roosters, and the semen collection was performed ten times. Macroscopic and microscopic evaluations were conducted to assess the chicken sperm. The collected data were analyzed using Analysis of Variance (ANOVA) followed by Duncan's multiple range test (DMRT).Result: The results of this study showed significant differences (P<0.05) in the sperm volume among the chicken breeds, with Birma (0.51 ml) and KUB chickens (0.60 ml) exhibiting distinct volumes compared to Arabic chicken (0.25 ml). Generally, the chicken sperm exhibited a milky white color and thick consistency. Microscopic evaluation yielded a mass motility rating of 3+. Meanwhile, individual motility and viability did not exhibit significant differences (P>0.05) among the three native chicken breeds. Abnormalities displayed significant variations (P<0.05), with Birma chicken showing the highest abnormality rate at 9.77%± 0.28. Sperm concentration did not exhibit significant differences (P>0.05), with the highest concentration observed in Arabic chicken at 4265.33±59.84 (x106 cells/ml). However, total sperm count and total motile sperm count exhibited significant variations (P<0.05), with the highest values recorded in KUB chicken at 2241.82±264.13 (x106 cells) and 1936.27±169.85 (x106 cells), respectively.Conclusions: In conclusion, the semen collection method using the female teaser yielded native chicken sperm with high quality and quantity and meets the required standards for successful insemination. This method holds potential and is recommended for application in native chicken breeding programs.
Co-Authors intan galuh bintari Junaedi Junaedi Kasianto Kasianto Muhammad Halim Natsir Nurul Isnaini Setya Budhi Udrayana