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Analisis kadar vitamin C pada buah naga putih (Hylocereus undatus) dan buah naga merah (hylocereus polyrhizus) dengan perbandingan metode spektrofotometri uv dan titrasi iodimetri Claudy Friesta Melanie; Gabena Indrayani Dalimunthe; Ridwanto; Fathur Rahman
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 3 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i3.216

Abstract

Dragon fruit is natural source of antioxidants. We can consume not only dragon fruit flesh, but the skin is also very beneficial for health, in reality it is only considered as waste that has not been used properly. This study aims to analyze the levels of vitamin C in white dragon fruit and red dragon fruit with a comparison of uv spectrophotometry and iodimetric titration methods. The stages of the research included extracting from dragon fruit, screening phytochemicals, testing levels of vitamin C using iodimetric titration, testing levels of vitamins using UV spectrophotometry. The results showed that red dragon fruit contains alkaloids, flavonoids, saponins, tannins, triterpenoids and glycosides, while white dragon fruit contains alkaloids, flavonoids, saponins and steroids. Determination of vitamin C levels by iodimetric titration method on red dragon fruit skin was 9.240 ± 3.029 mg, on red dragon fruit flesh was 11.587 ± 1.893 mg and vitamin C levels on white dragon fruit skin was 9.827 ± 3.933 mg, on white dragon fruit flesh was 13.347 ± 3.143 mg in 100 gram sample. While the spectrophotometric method obtained vitamin C levels in white dragon fruit flesh, namely 332.93 mg/100g, in red dragon fruit flesh, 24.99 mg/100g, while red dragon fruit skin, which was 16.59 mg/100g, in white dragon fruit skin, namely 10.47 mg/100g.
Penetapan kadar fenolik total ekstrak kayu bajakah (Spatholobus littolaris Hassk.) berdasarkan perbedaan konsentrasi etanol dengan metode spektrofotometri Uv-Vis Indah Triutami Harahap; Anny Sartika Daulay; Fathur Rahman; Haris Munandar Nasution
Journal of Pharmaceutical and Sciences JPS Volume 6 Nomor 4 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i4.302

Abstract

The root of the Bajakah tampala (Spatholobus littolaris Hassk.) is one of the plants that is empirically used by the people of the interior of Central Kalimantan as a traditional medicine. According to the preliminary tests conducted, Bajakah Tampala contains phenolics. Phenolic compounds are compounds that have antioxidant activity. The objective of this research was to determine the class of compounds contained in the macerated extract and the total phenolic content and yield of the macerated extract in Bajakah roots. The stages of this research included the processing of plant materials, preparation of macerated and pirated root extracts, characterization examination, phytochemical screening, and the effect on the yield of total phenolic content of Bajakah roots using the UV-Vis spectrophotometry method. The results showed that the simplicia powder of the root of the Bajakah wood contained alkaloids, glycosides, steroids and triterpenoids, flavonoids, phenolics, saponins, and tannins, while the ethanol extract of the roots of the Bajakah wood showed the presence of alkaloids, steroids and triterpenoids, tannins, saponins, flavonoids, and phenolic compounds. The determination of total phenolic content was carried out by determining the maximum wavelength of gallic acid and calculating the total phenolic content based on the difference in ethanol concentration using the UV-Vis spectrophotometry method. The results for determining total phenolic content in 96% ethanol extract were 33.872 ± 0.0420 mg GAE/g, 70% ethanol extract was 29.345 ± 0.2149 mg GAE/g, and 50% ethanol extract was 18.512 ± 0.1355 mg GAE/g. So it can be concluded that the total phenolic content of 96% ethanol extract is higher than that of 70% ethanol extract and 50% ethanol extract.