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All Journal Jurnal Fitopatologi Indonesia Agrikultura Akta Agrosia Dharma Raflesia : Jurnal Ilmiah Pengembangan dan Penerapan IPTEKS
Tunjung Pamekas, Tunjung
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Sekolah Lapang Pengelolaan Hama Terpadu (SLPHT) di Desa Sumber Agung Bengkulu Utara Apriyanto, Dwinardi; Pamekas, Tunjung; Nadrawati
DHARMA RAFLESIA Vol 20 No 2 (2022): DESEMBER (ACCREDITED SINTA 5)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/dr.v20i2.21471

Abstract

Produktivitas lahan sawah beririgasi di Desa Sumber Agung, Bengkulu Utara masih rendah. Petani mempraktekkan budidaya padi secara konvensional dengan masukan pupuk dan pestisida kimia. Sekolah lapang pengelolaan hama terpadu (SLPHT) dilakukan untuk meningkatkan pengetahuan tentang ekosistem tanaman dan produktivitas padi sawah, dan mengurangi penggunaan pestisida kimia. Kegiatan dilakukan pada demplot seluas +7.500 m2, untuk membandingkan sistem pengelolaan hama terpadu (PHT) dengan sistem konvensional. Petani peserta SLPHT dilatih melakukan pengamatan ekosistem tanaman setiap minggu selama satu musim tanam (11 kali pertemuan). Hasil kegiatan menunjukkan adanya peningkatan pengetahuan petani tentang ekosistem padi dan pengelolaan tanaman sehat dan hama penyakit tanaman hampir dua kali lipat dengan skor nilai dari 42.72 menjadi 83.52. Aplikasi pestisida nabati dan agensia hayati dilakukan pada petak PHT sebanyak 3 kali,  sedangkan aplikasi pada petak konvensional sebanyak 8 kali dengan menggunakan pestisida kimia. Serangan dari hama burung pipit yang tidak bisa ditanggulangi menyebabkan produktivitas rendah. Hasil ubinan gabah kering panen (GKP) pada petak PHT adalah 2.3 + 0.36 kg (setara 2.172 ton per Ha) sedangkan pada petak konvensional 2.06 + 0.38 kg (setara 1.94 per Ha).
Infection of Ageratum yellow vein virus on Weed Crassocephalum crepidioides in Bengkulu Marpaung, Nia Kurniati Br.; Sutrawati, Mimi; Ganefianti, Dwi Wahyuni; Novanda, Ridha Rizki; Pamekas, Tunjung
Jurnal Fitopatologi Indonesia Vol 19 No 1 (2023): Januari 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.1.39-44

Abstract

Beberapa jenis gulma dengan gejala infeksi virus ditemukan pada tiga sentra pertanaman pepaya (Carica papaya) di Provinsi Bengkulu, Indonesia. Gejala pada gulma ialah tulang daun dan lamina daun menguning, mosaik kuning, dan keriting. Gejala tersebut mirip dengan gejala infeksi Begomovirus pada beberapa jenis tanaman. Penelitian dilakukan dengan tujuan mendeteksi dan mengidentifikasi spesies Begomovirus pada spesies gulma Crassocephalum crepidioide. Deteksi Begomovirus dilakukan dengan metode polymerase chain reaction menggunakan sepasang primer universal Begomovirus, SPG1/SPG2. Pita DNA berukuran 912 pb berhasil diamplifikasi dari sampel gulma C. crepidioides dengan gejala daun keriting. Berdasarkan analisis Blastn, sampel Begomovirus asal gulma C. crepidioides memiliki kekerabatan yang paling dekat dengan Ageratum yellow vein virus (AYVV) isolat asal Taiwan (DQ866134.1) dengan homologi sebesar 99%. Hasil penelitian ini merupakan laporan pertama infeksi AYVV pada C. crepidioides di Indonesia.
Eksplorasi, Isolasi dan Identifikasi Jamur Entomopatogen Asal Rizosfer Tanaman Jagung di Bengkulu dengan Metode Baiting Insect Ginting, Sempurna br; Pamekas, Tunjung; Neildi, Zahara
Agrikultura Vol 35, No 2 (2024): Agustus, 2024
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v35i2.55390

Abstract

Jamur entomopatogen merupakan salah satu agen pengendali hayati yang dapat digunakan untuk mengendalikan serangga hama. Penggunaan jamur entomopatogen untuk pengendalian serangga memiliki kelebihan yaitu kapasitas produksi yang tinggi, siklus hidup yang relatif singkat dan ramah lingkungan. Penelitian ini bertujuan untuk mengeksplorasi, mengisolasi dan mengidentifikasi jamur entomopatogen asal rizosfer tanaman jagung di Bengkulu dengan metode baiting insect. Penelitian dilaksanakan dari bulan Maret hingga Agustus 2023 di lahan tanaman jagung di tiga lokasi yaitu Desa Taba Mulan, Desa Tebat Monok dan Desa Talang Kering di Kabupaten Kepahiang dan Kota Bengkulu. Identifikasi jamur dan uji patogenisitas dilakukan di Laboratarium Proteksi Tanaman, Fakultas Pertanian, Universitas Bengkulu. Metode yang digunakan yaitu isolasi tanah dari tanaman jagung dan trapping jamur entomopatogen menggunakan larva Tenebrio molitor. Jamur yang tumbuh menyelubungi tubuh larva yang terinfeksi diisolasi, diidentifikasi dan dilakukan uji patogenisitas terhadap larva T. molitor. Hasil eksplorasi, isolasi dan identifikasi diperoleh 12 isolat jamur entomopatogen yang terdiri dari 4 isolat berasal dari Lokasi 1 (Desa Taba Mulan, Kecamatan Meringgi), 6 isolat berasal dari Lokasi 2 (Desa Tebat Monok, Kecamatan Kepahiang) dan 2 isolat berasal dari Lokasi 3 (Desa Talang Kering, Kecamatan Muara Bangkahulu). Perbedaan karakteristik lingkungan ketiga lokasi mempengaruhi jumlah isolat yang diperoleh. Dari 12 isolat yang diperoleh terdapat 10 isolat yang teridentifikasi dan 2 isolat yang belum teridentifikasi yaitu 8 isolat Aspergillus sp. dan 2 isolat Fusarium sp. Laju pertumbuhan jamur entomopatogen yang cepat ditunjukkan oleh isolat CeTMM2, CeTBM2, dan CTBM3. Hasil uji patogenisitas selama 14 hari menunjukkan adanya larva T. molitor yang mati terinfeksi pada semua perlakuan isolat jamur entomopatogen yang diuji. Mortalitas larva terinfeksi berkisar antara 73,33-93,33% dengan mortalitas tertinggi terjadi pada isolat CeTBM5 dan terendah pada isolat CeTK1.
Responses of Solo Garlic Crops Grown at Low Elevation of Tropical Areas to Organic Matter and Paclobutrazol Suharjo, Usman Kris Joko; Widodo; Pamekas, Tunjung; Monica
Akta Agrosia Vol 27 No 1 (2024)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/aa.27.1.32-36

Abstract

Solo garlic has been known for its medicinal benefits because of which it is considerably expensive. Nonetheless, no Indonesian farmer produces solo garlic intentionally since no information on how to grow solo garlic in Indonesia is available.  Growers, usually harvest 5-10 kg of solo garlic from 1 hectare of garlic crop they grow. A greenhouse experiment was conducted from November 2018 to January 2019 at the Department of Agronomy, Faculty of Agriculture, Bengkulu University, about 10 m above sea level to study the effects of paclobutrazol and organic matter on the เว็บสล็อต | R4shub growth and yield of solo garlic crop grown at low elevation of Bengkulu. The experiment used completely randomized design arranged in factorial (2 factors; 5 replications). The first factor tested was organic matter levels (0, 2.5, 5.0, 7.5%) and the second factor was paclobutrazol concentrations (0, 500, 1000, 1500 ppm). Solo garlic seeds were planted in the polybag filled with 5 kg of media, a mixed of topsoil and organic matter. The crops were sprayed with paclobutrazol at 21, 35, and 49 days after planting.  The results showed that organic matter did not significantly affect crop growth and yield. In contrast, paclobutrazol significantly affected crop growth and yield, with the best yield was found at 1500 ppm paclobutrazol. Furthermore, there was no significant interaction between organic matter and paclobutrazol on the growth and yield of solo garlic grown at low elevation at tropical climate in Indonesia. Keywords: clove, medicine, organic matter, paclobutrazol, solo garlic
Responses of six tomato (Lycopersicon esculentum Mill.) genotypes to salinity stress at low altitudes of Bengkulu, Indonesia Suharjo, Usman KJ; Nababan, Sartika Y; Masdar, Masdar; Pamekas, Tunjung; Mukhtasar, Mukhtasar
Akta Agrosia Vol 21 No 1 (2018)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.374 KB)

Abstract

Salinity stress causing water deficit, nutrient deficiency, and ion imbalance for the crops has been reported to reduced crop growth, crop yield, and fruit quality in tomatoes. The main objective of this research were to determine the LC-50 of salinity, to evalute the response of six tomato genotypes to LC-50, and to study the effect of genotype and salinity stress on crop growth, crop yield, and fruit quality of tomatoes. The LC-50 of salinity was determinated by growing tomato seedlings at different level of saline solutions (0, 400, 500, 6000, 7000, 9000, 10000, 11000, and 12000 ppm of NaCl) and used the LC-50 of salinity to evaluate the salinity tolerance of six tomato genotypes. The results showed that the LC-50 of saline solution was 9000 ppm NaCl and genotype Empat G was found to be the most tolerant to salinity stress
Promoting Tuber Formation In Vitro with Benzyl Amino Purine and Paclobutrazol at Different Concentrations Suharjo, Usman Kris Joko; Hasanudin, Hasanudin; Pamekas, Tunjung; Pujiwati, Hesti; Vanturini, Alyi
Akta Agrosia Vol 22 No 1 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (884.775 KB)

Abstract

A common problem related to the production of potatoes in Indonesia is the use of poor quality seed sources and declining seeds. Plant tissue culture is one of the best methods used for the provision of quality seeds. This study aims to establish the best combination of BAP concentration and the concentration of paclobutrazol in spurring the growth of cuttings and initiation of potato tubers in vitro.This research was conducted in November 2017 until June 2018 at Agronomy Laboratory of Biotechnology Division and Plant Culture Network of Agriculture Faculty of Bengkulu University. The  research  design  used  was  Completely  Randomized  Design  (CRD)  which  consist  of  18 combination of BAP treatment and Paclobutrazol was repeated 6 times. The concentration of BAP used was 0 mg / l, 5 mg / l, 10 mg / l and the concentration of Paclobutrazol 0 mg / l; 2.5 mg / l; 5 mg / l; 7.5 mg / l; 10 mg / l; 12.5 mg / l. The data of the research were analyzed by F test of 5% level and if there were different between treatments followed by Duncan's Multiple Range Test (DMRT) test of5% level. The results showed that the combination of BAP and  Paclobutrazol concentration had significant effect on all observed variables. Treatment of 5 mg / l BAP and 7.5 mg / l Paclobutrazol is the best medium in inducing potato micro tube as indicated by the fastest tuber formation time, highest percentage of productive crops, largest number of tubers per bottle, number of tubers per productive plant, tuber diameter the largest and highest wet weight of tuber per bottle. Keywords : in vitro, BAP, paclobutrazol, Potato
Co-Authors Dwi Wahyuni Ganefianti, Dwi Wahyuni Dwinardi Apriyanto Ginting, Sempurna br Hasanudin Hasanudin HESTI PUJIwATI Marpaung, Nia Kurniati Br. Masdar Masdar Mimi Sutrawati Monica Mukhtasar, Mukhtasar Nababan, Sartika Y Nadrawati Neildi, Zahara Novanda, Ridha Rizki Suharjo, Usman KJ Suharjo, Usman Kris Joko Vanturini, Alyi Widodo