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All Journal Jaringan Laboratorium Medis Journal of Applied Health Management and Technology
Devi Etevia Purlinda
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Public Health

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Detection of rpoB Gene Mutations in Mycobacterium Tuberculosis widodo widodo; Ahmad Riadi; Devi Etevia Purlinda; Lilik Setyowatiningsih; roni roniafriansya
Journal of Applied Health Management and Technology Vol 5, No 3 (2023): July 2023
Publisher : Politeknik Kesehatan Kementerian Kesehatan Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jahmt.v5i3.10093

Abstract

Perkembangan pengobatan infeksi tuberculosis memberikan harapan kesembuhan total akan tetapi seiring berjalanya waktu pengunaan obat berdampak pada munculnya strain Mycobacterium tuberculosis yang resisten terhadap obat peningkatan kecepatan diaknostik terhadap resistensi perlu dikembangkan salah satunya methode molekuler berbasis PCR penentu mutasi gen yang disebakan resistensi obat salah satu target obat yang digunakan adalah rpoB, pengunaan Primer rpoB sebagai penanda terjadinya mutasi diharapkan mampu memberikan dampak sebagai alternatif deteksi cepat secara molekuler. Panjang pita rpoB 318 bp cocok untuk kelompok Mycobacterium tuberculosis diperoleh hasil menunjukan pada kodon 509 perubagan AGC menjadi AAT terjadi perubahan asam amino dari serin menjadi Apargarin, perubahan nukleotida  CAG menjadi CCG pada kodon 510 mengubah asam amino Glutamin menjadi prolin, kodon 514 perubahan nukleotida TTC menjadi TTG mengubah asam amino Phenylalanine menjadi leucine, kodon 515 terjadi perubahan nukleotida ATG menjadi GTG perubahan ini menyebakan perubahan asam amino Methionine menjadi Valine  . perubahan tersebut menyebabkan terjadinya resistensi terhadap obat Rifampicin
Perbandingan Berbagai Metode Pengamatan Mycobacterium Tuberculosis secara Mikroskopis Widodo Widodo; Tri Sugiyatmini; Devi Etevia Purlinda
Jaringan Laboratorium Medis Vol 6 No 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.12137

Abstract

Technological developments are increasingly rapid in the field of identification and special equipment in the field of microbiology, the development of staining methods and microscopic observation is growing rapidly. The Ziehl Neelsen staining method is still an alternative follow-up to other alternative treatments using the Auramin O staining method with flurocene microscope observation. This research is an analytical observational study and a cross sectional research design to see the differences in readings between Ziehl Neelsen staining, flurocene auramin and gram staining. We used isolates that had been identified as Mycobacterium tuberculosis which we obtained from Balepkes and Pak provinces of Central Java under non-activation conditions, by autoclaving at 121 degrees for 15 minutes. The number of samples was 10 slides with Ziehl Neelsen staining, Flurocene Auramin and Gram staining. The results of the sensitivity and specificity research between Ziehl Neelsen and auramin O were 100%. Meanwhile, the gram staining method and SEM observation cannot be compared because they can only observe bacterial morphology. From the research results it can be concluded that Auramin O can be used as an alternative Ziehl Neelsen painting agent.
Co-Authors Ahmad Riadi Lilik Setyowatiningsih Roni Afriansya Tri Sugiyatmini Widodo Widodo widodo widodo