Rosita Dwi Chandra, Rosita Dwi
Ma Chung Research Center for Photosynthetic Pigments (MRCPP) - Universitas Ma Chung

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Economically Potential Pigments from Marine Blue-Green Algae for the Application in Food and Health Salim, Katarina Purnomo; Chandra, Rosita Dwi; Heriyanto, Heriyanto; Susilaningsih, Dwi; Limantara, Leenawaty; Brotosudarmo, Tatas H.P.
Indonesian Journal of Natural Pigments Vol 1 No 2 (2019): Agustus 2019
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2019.01.2.37

Abstract

Current efforts to explore the potency of marine resources have been made to promote economic development in Indonesia, especially in the industrial sector. Marine cyanobacteria such as Arthospira sp. and Scytonema sp. are two potential candidates of the blue-green algae that can be used in the application for food and health industries. This study focused on the identification of the composition of the dominant chlorophylls and carotenoids. The results showed the presence of Zeaxanthin, Chlorophyll a, and β-carotene. The economic potency of those dominant pigments will be reviewed in this article.
Isolation, Encapsulation, Stability and Characteristics of Thylakoid from Suji Leaves (Pleomele angustifolia) as Natural Food Coloring Agent Chandra, Rosita Dwi; Indrawati, Renny; Heriyanto, Heriyanto; Brotosudarmo, Tatas H.P.; Limantara, Leenawaty
Indonesian Journal of Natural Pigments Vol 1 No 2 (2019): Agustus 2019
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2019.01.2.53

Abstract

Suji (Pleomele angustifolia) is one kind of Indonesian typical plants which can be used as natural green food coloring agent. The susceptibility of natural pigment to external environment forces the protection in order to prolong its shelf life. Encapsulation has been known in the art of food preparation to provide protection for several ingredients including food coloring agent. The objective of this study was to observe the method for isolation and encapsulation of thylakoid, and to investigate the stability and characteristics of thylakoid of suji leaves encapsulated in maltodextrin during dark storage at 30 °C, 45 °C, and 60 °C. The degradation of the encapsulated pigments was identified through chromametric analysis which resulted in the increase of L* (lightness), a* (redness), and b* (yellowness) values. In addition, it was also indicated by the decrease of total chlorophyll (TC) which was determined using spectrophotometer. Chromatography analysis confirmed the presence of four major peaks in the fresh encapsulated thylakoid powder and five major peaks in the encapsulated thylakoid powder stored at the highest temperature (60 °C), with Chl a as the dominant pigments in both powder. The vivid green powder was able to preserve its color without any obvious change to an untrained eye up to 60 d of storage at 30 °C, becoming a promising ingredient to replace the synthetic colorants.
Color Alteration of Encapsulated Beetroot (Beta vulgaris L.) Extract Upon Dissolving in Various pH Treatment Lukitasari, Diah Mustika; Indrawati, Renny; Chandra, Rosita Dwi; Heriyanto, Heriyanto; Limantara, Leenawaty
Indonesian Journal of Natural Pigments Vol 2 No 2 (2020): Agustus 2020
Publisher : Ma Chung Research Center for Photosynthetic Pigments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33479/ijnp.2020.02.02.48

Abstract

It has been known that most natural pigments are unstable upon exposure against several environmental factors, such as thermal treatment, light, oxidizing or reducing agents, as well as acid or alkaline compounds. Encapsulation procedures is often adopted to adjust the solubility and provide protection to the natural pigments. Here, we prepared an encapsulated beetroot extract as the candidate of red bio-colorant. The primary aim of the present study is to investigate color alteration of encapsulated beetroot (Beta vulgaris L.) extract upon dissolving in various pH adjustment and prolonged storage. The McIllvainne buffer was prepared in various pH range, i.e. from 2 to 11. The encapsulated extract (0.1% w/v) was dissolved and the color of the solutions were measured regularly until 3 days storage under darkness at 200C. Any degradation or structural changes will cause color alteration, which were monitored through L*, a*, b* values, the hue angle (H°), chroma values (C), as well as color difference (DE). The results showed that pH 4 was the most favorable condition that brings least impact to the color alteration, even when the colored solution was kept in prolonged storage until 9 days.