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All Journal Jurnal Akuakultur Indonesia Media Akuakultur Journal of Rural and Urban Community Empowerment Dinamika Pertanian Jurnal Agroteknologi Agribisnis dan Akuakultur
Khairul Hadi
Universitas Islam Riau
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology

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PEMANFAATAN FERMENTASI LINDI SAMPAH PERKOTAAN DALAM MEDIA KULTUR TERHADAP BIOMASSA DAN KOMPOSISI KIMIA Chlorella sp. Hadi, Khairul; Rosyadi, Rosyadi
Media Akuakultur Vol 19, No 2 (2024): Desember 2024
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.19.2.2024.71-79

Abstract

Lindi merupakan cairan yang terbentuk dalam timbunan sampah dan berpotensi sebagai pencemar lingkungan. Namun apabila diolah lindi dapat menjadi sumber unsur hara untuk kultur Chlorella sp.. Penelitian ini dilakukan untuk mengetahui pengaruh lindi dalam media kultur dan mendapatkan konsentrasi lindi terbaik untuk meningkatkan biomassa serta komposisi kimia Chlorella sp.. Penelitian menggunakan rancangan acak lengkap dengan 5 perlakuan, yaitu pemberian lindi 5, 10, 15, 20 dan 25% dan masingmasing perlakuan terdiri dari 3 ulangan. Sebelum digunakan, lindi disaring dan dicampur dengan 20 g gula merah yang dihancurkan, 1 L akuades, 15 mL Effective Microorganism4 (EM4 ), dan kemudian difermentasi selama 1 hari. Kultur dilakukan dalam galon berkapasitas 20 L yang diisi dengan 16 L air tawar, dengan penerangan menggunakan lampu dengan intensitas 2500 lux. Proses kultur berlangsung selama 20 hari, dengan parameter yang diukur meliputi kepadatan sel, biomassa, kandungan proksimat, asam amino, kadar nitrat, fosfat, dan kualitas air (suhu, pH, dan DO). Hasil penelitian menunjukkan pemberian lindi dalam media kultur berpengaruh nyata terhadap biomassa Chlorella sp. (p<0,05) dengan konsentrasi lindi terbaik pada perlakuan 25%, yaitu biomassa sebesar 0,44 ± 0,00 g L-1, mengandung protein 31,208%, lemak 7,125%, karbohidrat 31,217%, kadar air 26,975% dan kadar abu 3,475%. Temuan ini menunjukkan bahwa lindi dapat digunakan sebagai alternatif sumber nutrisi untuk meningkatkan produktivitas Chlorella sp., dengan konsentrasi 25% sebagai yang paling optimal.Leachate is a liquid that forms in landfills and can be an environmental pollutant. However, if the leachate is processed, it can become a source of nutrients for Chlorella sp. culture. This research was conducted to determine the effect of leachate in the culture medium and to obtain the best leachate concentration to increase the biomass and chemical composition of Chlorella sp.. The research used a completely randomized design with five treatments, namely the addition of 5%, 10%, 15%, 20%, and 25% leachate, with each treatment consisting of three replications. Before use, the leachate was filtered and mixed with 20 g of crushed brown sugar, 1 L of distilled water, 15 mL of Effective Microorganism 4 (EM4 ), and then fermented for 1 day. The culture was conducted in a 20 L gallon filled with 16 L of freshwater, illuminated with a light intensity of 2500 lux. The culturing process lasted for 20 days, with parameters measured including cell density, biomass, proximate content, amino acids, nitrate, phosphate, and water quality (temperature, pH, and DO). The results showed that the addition of leachate in the culture media had a significant effect on the biomass of Chlorella sp. (p<0.05), with the best leachate concentration being 25%, yielding a biomass of 0.44±0.00 g L-1 , containing 31.208 % protein, 7.125 % lipid, 31.217 % carbohydrates, 26.975 % moisture content, and 3.475% ash content. These findings indicate that leachate can be used as an alternative nutrient source to enhance the productivity of Chlorella sp., with a 25% concentration being the most optimal
Co-Authors Agusnimar, Agusnimar dedy dedy, dedy Dwi Cahya, Fihlia Fakhrunnas MA Jabbar Faradila Ikhfanisa Gusna Meli Roza Hasibuan, Afap Jarod Setiaji Muhammad Hasby Muhammad Nur Rasidi, Abdul Fatah Rosyadi Rosyadi Rosyadi, Rosyadi Sulhaswardi, Sulhaswardi Suprayitno Hamdan T. Iskandar Johan Teguh Oktavian