Enty
Department Of Microbiology, Catholic University Of Atmajaya Medical School, Jakarta

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Infeksi Luka Operasi (ILO) di Bangsal Kebidanan dan Kandungan RSUPN Ciptomangunkusumo (RSCM): Laporan Serial Kasus Bulan Agustus-Oktober 2011 Wardoyo, Eustachius Hagni; Tjoa, Enty; Ocvyanty, Dwiana; H Moehario, Lucky
Cermin Dunia Kedokteran Vol 41, No 5 (2014): Muskuloskeletal
Publisher : PT. Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.969 KB) | DOI: 10.55175/cdk.v41i5.1135

Abstract

Tujuan: Mendeskripsikan kejadian Infeksi Luka Operasi (ILO) di bangsal Kebidanan dan Kandungan RSCM selama bulan Agustus- September 2011. Metode: Deskripsi analitik terhadap data rekam medik di bagian Kebidanan dan Kandungan RSCM dengan cara purposive sampling. Kejadian ILO yang dilaporkan oleh tim pengendalian infeksi rumah sakit (PPIRS) dimasukkan sebagai kriteria inklusi. Hasil: Infeksi Luka Operasi (ILO) di Bangsal Kebidanan dan Kandungan RSCM selama Agustus-Oktober 2011 memiliki insidensi 25/566 (4,4%). Mikroorganisme penyebab ILO: E. coli (7), Acinetobacter spp. (2), Staphylococcus epidermidis (2), Pseudomonas spp., Enterobacter aerogenes, Klebsiella oxytoca, Klebsiella pneumonia, Staphylococcus aureus, Streptococcus non hemolitikus, grup D masing-masing 1, Staphylococcus sp. & Bacillus sp. (1), Klebsiella pneumonia & Pseudomonas sp. (1) dan E.coli & Acinetobacter sp (1). Simpulan: Insidensi ILO bangsal Kebidanan dan Kandungan pada Agustus – Oktober 2011 dilaporkan 4,4%. E. coli merupakan organisme penyebab ILO terbanyak.Objective: To describe the incidence of surgical site infection (SSI) in Obstetric and Gynaecologic Ward Cipto Mangunkusumo National Referral Hospital (RSCM) during August to September 2011. Method: Analytic description on medical record from Obstetric and Gynaecologic Ward obtained by purposive sampling methods. The incident of SSI reported by RSCM infection control team was included. Result: The incidence of SSI during August to September 2011 was 25/566 (4,4%). The organism detected were: E. coli (7), Acinetobacter spp. (2), Staphylococcus epidermidis (2), Pseudomonas spp., Enterobacter aerogenes, Klebsiella oxytoca, Klebsiella pneumonia, Staphylococcus aureus, Streptococcus non hemolitikus, grup D one case each, Staphylococcus sp. & Bacillus sp. (1), Klebsiella pneumonia & Pseudomonas sp. (1) dan E.coli & Acinetobacter sp (1). Conclusion: The incidence of SSI in Obstetric and Gynaecologic ward during August to September 2011 was 4.4%. E. coli was the organism most frequently isolated.
Hand hygiene knowledge, perception, and compliance among healthcare workers Enty Tjoa; Cipta Mahendra; Suryanto Suryanto; Sandy Theresia; Marcella Wirjanata; Daniel Ardian Soeselo
International Journal of Public Health Science (IJPHS) Vol 11, No 2: June 2022
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v11i2.21263

Abstract

Healthcare-associated infections (HAIs) is a great issue to concern in healthcare services because it accounts for prolonged hospital stay and may lead to morbidity or mortality. Proper hand hygiene behavior in the healthcare environment is indispensable in minimizing the risk of HAIs. This study aimed to identify the impact of hand hygiene education on healthcare workers' (HCWs) compliance, knowledge, and perception of hand hygiene at Atma Jaya Hospital, Jakarta, Indonesia. The compliance of hand hygiene was evaluated using the World Health Organization’s (WHO) five moments for hand hygiene as the reference. Data on hand hygiene knowledge and perception were collected using a translated version of the WHO’s hand hygiene knowledge questionnaire and hand hygiene perception questionnaire, respectively. Results showed a significant increase in compliance with hand hygiene after the online session’s intervention had been implemented, except hand hygiene compliance for the ‘after touching patient surroundings’ moment. There was also a significant increase in the HCWs' hand hygiene knowledge after the intervention. The HCWs' perceptions are also found to be positive even before the intervention. This study concludes that the HCWs' knowledge and compliance with hand hygiene at Atma Jaya Hospital increase significantly after an educational intervention had been conducted.
Screening of Legionella pneumophila from water sources in the hospitals in Jakarta Lucky Moehario; T. Robertus; Yuliana Grace; Enty Tjoa
Health Science Journal of Indonesia Vol 10 No 1 (2019)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v10i1.1852

Abstract

Latar belakang: Pneumonia akibat bakteri Legionella masih menjadi masalah di berbagai tempat di dunia; menjadi penyebab 2-15 % dari pneumonia yang perlu di rawat di Rumah Sakit. Kasus legionellosis di Indonesia dilaporkan terjadi di Bali pada tahun 1996 dan di Tangerang tahun 1999. Keberadaan Legionella di fasilitas Pelayanan Kesehatan berpotensi sebagai penyebab infeksi nosokomial. Bakteri Legionella hidup di lingkungan perairan hangat dan lembab, juga ditemukan diberbagai sumber air seperti, sumber air sistem pendingin ruangan, kolam renang, tempat penampungan air di rumah sakit, perkantoran, hotel, dan perumahan sehingga turut berkontribusi dalam terjadinya community acquired dan pneumonia nosokomial. Metode: Penelitian ini merupakan penelitian deskriptif yang bertujuan untuk penapisan keberadaan Legionella pneumophila diberbagai sumber dan penampungan air di Rumah Sakit (RS) di Jakarta dengan menggunakan medium Legionella Charcoal Yeast Extract (CYE) dan dengan berbagai suplemen. Tujuh belas sampel air yang berasal dari berbagai sumber air di dua RS yang berlokasi diJakarta Utara dan Barat telah diteliti. Hasil: Dua puluh satu koloni yang ditemukan memiliki karakterisitik L. pneumophila dari semua varian medium, namun pada tes agglutinasi latex tidak memberikan reaksi positif. Kesimpulan: L. pneumophila tidak ditemukan diberbagai sumber air dari dua RS ini. Penggunaan metode yang lebih sensitif dan spesifik perlu dilakukan untuk memastikan ditemukannya L. pneumophila. Kata kunci: Legionellosis, Legionella pneumophila, medium BCYE Abstract Background: Pneumonia due to Legionella bacteria is still a problem in various places in the world, causes 2 15% of pneumonia that need hospitalization. In Indonesia, legionellosis cases have been reported in Bali in 1996 and Tangerang in 1999. The existence of Legionella in healthcare facilities is potential to cause nosocomial infections. Legionella bacteria live in warm and humid waters, and are also commonly found in various water sources, such as water cooling systems, swimming pools, water reservoirs in hospitals, offices, hotels and housing. These bacteria contribute to the occurrence of community-acquired and nosocomial pneumonia. Methods: This study was a descriptive research, and aimed to screen water sources and reservoirs in the hospitals in Jakarta for the existence of Legionella pneumophilla using Legionella Charcoal Yeast Extract (CYE) medium with various supplements. A total of 17 water samples from 2 hospitals located in West and North Jakarta have been examined. Results: The results showed a total of 21 colonies with characteristics as of L. pneumophila were obtained from those water samples, however, none showed positive results in the latex agglutination test. Conclusion: L. pneumophila was not found thus far in the water sources in these two hospitals. A more sensitive and specific approaches might be used to enable the findings of L. pneumophila. Keywords: Legionellosis, Legionella pneumophila, BCYE medium
Comparison of BCYE and BMPA media on recovery rate of Legionella pneumophila Lucky Moehario; Enty Tjoa; Mutiara J. Taslim; Yohanna Angelina
Health Science Journal of Indonesia Vol 11 No 1 (2020)
Publisher : Sekretariat Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/hsji.v11i1.3127

Abstract

Latar belakang: Legionella pneumophila (L. pneumophila) telah banyak diketahui sebagai penyebab legionellosis; habitat nya di berbagai sumber air, lingkungan lembab dan hangat. Metode kultur menggunakan media spesifik masih menjadi baku emas untuk identifikasi L. pneumophila. Penelitian ini bertujuan membandingkan recovery rate L. pneumophila ATCC®33823 pada medium spesifik BCYE, dan medium selektif BMPA. Metode: Dilakukan dilusi serial pada suspensi 0,5 McFarland L. pneumophila ATCC®33823; 100 ul dari tiap tingkat dilusi diinokulasi pada medium Buffered Charcoal Yeast Extract (BCYE) dan medium BMPA (medium BCYE yang ditambahkan suplemen BMPA-α), secara duplo. Konsentrasi suspensi dihitung menggunakan metode Angka Lempeng Total (ALT) dari Standar Nasional Indonesia No. 01-2332.3-2006. Persentase recovery rate dihitung dan dianalisis secara statistik menggunakan SPSS versi 23,0. Hasil: Jumlah koloni L. pneumophila yang tumbuh pada medium BMPA jauh lebih tinggi daripada yang tumbuh di medium BCYE; konsentrasi tertinggi yang diperoleh adalah pada medium BMPA sebesar 1,45 x 107 CFU/ml. Persentase recovery rate pada medium BMPA adalah 96,67%, dan 60,67% pada medium BCYE. Kesimpulan: Recovery rate medium BMPA untuk pertumbuhan koloni L. pneumophila ATCC®33823 jauh lebih tinggi daripada media BCYE, karena itu medium BMPA dapat direkomendasikan untuk kultivasi L. pneumophila, khususnya pada program surveilans berbagai sumber air dengan biaya lebih terjangkau. Kata kunci: Legionella pneumophila, medium spesifik, BCYE , BMPA, recovery rate Abstract Background: Legionella pneumophila (L. pneumophila) has been known as the etiology of legionellosis; they live in aquatic environment, warm and moist. Culture method using specific medium remains as the gold standard in the identification of L. pneumophila. This study aimed to compare the recovery rate of L. pneumophila ATCC® 33823 on the specific medium BCYE for the cultivation of Legionella, and BMPA, the selective medium. Methods: Suspension of L. pneumophila ATCC® 33823 of 0.5 McFarland was diluted to 10 fold serial dilution; 100 ul of each dilution was inoculated on Buffered Charcoal Yeast Extract (BCYE) medium, and BMPA (BCYE supplemented with BMPA-α) in duplicate manner. The concentration was calculated using Total Plate Count standard as of Indonesian Nasional Standard number 01-2332.3-2006. The percentage of recovery rate was calculated, and the statistical analysis was performed using SPSS version 23.0. Results: Numbers of colonies of L. pneumophila grew on BMPA was much higher than on BCYE medium; the highest concentration was yielded on BMPA medium i.e. 1.45x107 CFU/ml. The recovery rates were 96.67% and 60.67% on BMPA medium and BCYE subsequently. Conclusion: The recovery rate of the BMPA medium on the colony growth of L. pneumophila ATCC®33823 was markedly higher than the BCYE, therefore BMPA medium can be suggested to be used in the cultivation of L. pneumophila especially in the routine surveillance program for water sources with less cost. Keywords: Legionella pneumophila, specific medium, BCYE , BMPA, recovery rate
Comparison of alcohol, povidone-iodine and octenidine dihydrochloride as skin disinfectants to reduce bacterial count prior to peripheral venous catheter insertions in newborn infants Lily Rundjan; Rinawati Rohsiswatmo; Sarah Rafika; Enty Enty; Lucky H. Moehario
Paediatrica Indonesiana Vol 51 No 5 (2011): September 2011
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.481 KB) | DOI: 10.14238/pi51.5.2011.277-81

Abstract

AbstractBackground Vascular access may increase the risk of bloodstream infections, especially in newborn infants with weak immune systems and requiring invasive supportive care. Skin disinfection prior to peripheral venous catheter insertion lowers the risk of infection. However, antiseptics chosen for this task should be effective and safe for newborn infants.Objective To compare the effectiveness of 70% alcohol (BD alcohol swabs ®), 10% povidone-iodine (Pharma-RSUPNCM), and octenidine (Octenisept ®) as antiseptics for reducing skin bacteria for pre-invasive procedures in neonates.Methods Infants aged less than 28 days, regardless of gestational age, at the Neonatal Unit of Cipto Mangunkusumo Hospital (RSUPNCM) were included in our study. Infants were divided into three groups, each tested with different skin antiseptics (alcohol, povidone-iodine or octenidine). Skin swabs were performed before and after application of skin antiseptic, followed by inoculation onto blood agar plates. Colony-forming units were counted after 18 hours of incubation at 37ºC.Results Ninety subjects were divided into 3 groups of 30, each group using either 70% alcohol swabs, 10% povidone-iodine, or octenidine as skin antiseptic. Skin swabs were taken before and after antiseptic application and drying, as well as 5 minutes after application. The mean reductions in CFU/cm2 (%) after antiseptic application (and fully dried) were 97.54% for povidone-iodine, 97.52% for octenidine, and 89.07% for alcohol. There were no significant differences in mean CFU reductions among the three antiseptics groups (P=0.299). Furthermore, 5 minutes after application, there were still no significant differences in the three antiseptic groups (P=0.289).Conclusions Although octenidine showed a significant bacterial count reduction after application, it was not significantly different from those of alcohol or povidone-iodine. [Paediatr Indones. 2011;51:277-81].
Infeksi Luka Operasi (ILO) di Bangsal Kebidanan dan Kandungan RSUPN Ciptomangunkusumo (RSCM): Laporan Serial Kasus Bulan Agustus-Oktober 2011 Eustachius Hagni Wardoyo; Enty Tjoa; Dwiana Ocvyanty; Lucky H Moehario
Cermin Dunia Kedokteran Vol 41, No 5 (2014): Muskuloskeletal
Publisher : PT. Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v41i5.1135

Abstract

Tujuan: Mendeskripsikan kejadian Infeksi Luka Operasi (ILO) di bangsal Kebidanan dan Kandungan RSCM selama bulan Agustus- September 2011. Metode: Deskripsi analitik terhadap data rekam medik di bagian Kebidanan dan Kandungan RSCM dengan cara purposive sampling. Kejadian ILO yang dilaporkan oleh tim pengendalian infeksi rumah sakit (PPIRS) dimasukkan sebagai kriteria inklusi. Hasil: Infeksi Luka Operasi (ILO) di Bangsal Kebidanan dan Kandungan RSCM selama Agustus-Oktober 2011 memiliki insidensi 25/566 (4,4%). Mikroorganisme penyebab ILO: E. coli (7), Acinetobacter spp. (2), Staphylococcus epidermidis (2), Pseudomonas spp., Enterobacter aerogenes, Klebsiella oxytoca, Klebsiella pneumonia, Staphylococcus aureus, Streptococcus non hemolitikus, grup D masing-masing 1, Staphylococcus sp. & Bacillus sp. (1), Klebsiella pneumonia & Pseudomonas sp. (1) dan E.coli & Acinetobacter sp (1). Simpulan: Insidensi ILO bangsal Kebidanan dan Kandungan pada Agustus – Oktober 2011 dilaporkan 4,4%. E. coli merupakan organisme penyebab ILO terbanyak.Objective: To describe the incidence of surgical site infection (SSI) in Obstetric and Gynaecologic Ward Cipto Mangunkusumo National Referral Hospital (RSCM) during August to September 2011. Method: Analytic description on medical record from Obstetric and Gynaecologic Ward obtained by purposive sampling methods. The incident of SSI reported by RSCM infection control team was included. Result: The incidence of SSI during August to September 2011 was 25/566 (4,4%). The organism detected were: E. coli (7), Acinetobacter spp. (2), Staphylococcus epidermidis (2), Pseudomonas spp., Enterobacter aerogenes, Klebsiella oxytoca, Klebsiella pneumonia, Staphylococcus aureus, Streptococcus non hemolitikus, grup D one case each, Staphylococcus sp. & Bacillus sp. (1), Klebsiella pneumonia & Pseudomonas sp. (1) dan E.coli & Acinetobacter sp (1). Conclusion: The incidence of SSI in Obstetric and Gynaecologic ward during August to September 2011 was 4.4%. E. coli was the organism most frequently isolated.
Prevalence of Cutibacterium acnes and Staph spp. in the lesions of acne vulgaris in Jakarta Hermawan, Melyawati; Tjoa, Enty; Hidajat, Inneke J.; Teressa, Maria; Layadi, Eka B.; Wolter, Alegra
Journal of General - Procedural Dermatology and Venereology Indonesia Vol. 5, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Acne vulgaris (AV) is a chronic skin disorder that is commonly seen in young adults. Recent studies have shown that bacteria other than Cuticubacterium acnes found in the skin microbiota also play vital roles in the pathogenesis of AV. Understanding the skin microbiota and its disruption in patients with AV is paramount in treating patients with AV. Methods: Samples were obtained from 36 subjects to evaluate the proportion of Cutibacterium acnes, Staphylococcus epidermidis, and Staphylococcus aureus in lesions of AV by means of bacterial culture. Results: There were 69 specimens collected from the 36 subjects with AV. The most abundant type of bacteria that was successfully isolated in the cultured AV lesions was Staphylococcus epidermidis(46.4%), followed by Cutibacterium acnes(14.5%), and Staphylococcus aureus(2.9%). Conclusion: Staphylococcus epidermidis,the major species of human skin flora, is the most abundant microorganism found in AV. The development of AV is not necessarily associated with the high proportion of Cutibacterium acnes. More pathogenic bacteria, such as Staphylococcus aureusis also not always found in the AV lesions. These findings may provide the basis for the clinicians to determine which types of antibiotics to prescribe for the treatment of AV.
Viabilitas Bacillus Subtilis di Media Preservasi Agar Skim Milk pada Tiga Varian Suhu Penyimpanan yang Berbeda Sheila Febrilia; Enty
Bahasa Indonesia Vol 22 No 1 (2023): Damianus Journal of Medicine
Publisher : Atma Jaya Catholic University of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25170/djm.v22i1.2634

Abstract

Pendahuluan: Preservasi bakteri merupakan hal yang umum pada kebanyakan laboratorium mikrobiologi. Berbagai metode seperti kering beku dan penyimpanan suhu rendah dianggap sebagai metode preservasi yang ideal, tetapi metode ini membutuhkan alat dan operator khusus. Agar Susu Skim merupakan media yang umum digunakan untuk preservasi bakteri. B.subtilis merupakan bakteri yang mudah tumbuh pada kebanyakan media. Studi ini bertujuan untuk mengidentifikasi viabilitas B.subtilis ATCC 6633 yang disimpan pada 3 suhu berbeda selama 150 dan 270 hari. Metode: Isolat B.subtilis ATCC 6633 dikultivasi ke dalam tabung berisi agar susu skim, yang kemudian akan dibagi menjadi 3 kelompok penyimpanan berdasarkan suhu yang telah ditentukan. Uji viabilitas akan dilakukan sebanyak 2 kali pada hari ke-150 dan 270 dengan menggunakan metode Miles & Misra. Hasil uji akan dicatat dan dianalisa dengan menggunakan uji General Linear Model pada aplikasi SPSS. Hasil: Viabilitas bakteri B.subtilis ATCC 6633 pada hari ke-150 dan 270 menunjukkan hasil yang sama yaitu bakteri tetap viabel. Secara statistik, tidak ada pengaruh yang bermakna oleh media preservasi dan suhu penyimpanan terhadap pertumbuhan bakteri B.subtilis (p>0,005). Simpulan: Tidak ada pengaruh yang bermakna antara media preservasi dan suhu penyimpanan terhadap pertumbuhan bakteri B.subtilis ATCC 6633. Kata Kunci: viabilitas bakteri, B.subtilis ATCC 6633, agar susu skim.