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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya Asian Journal of Health and Applied Sciences
Wahid, Aziz Ansori
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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Comparison of hemoglobin level measurement results using sodium lauryl sulphate with Oshiro and Mansoor procedure Fadhilah, Wasiyah Khusna; Al Badri, Muhammad Hilal; Wahid, Aziz Ansori; Fadhila, Feldha
Asian Journal of Health and Applied Sciences Vol. 1 No. 1 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.919 KB) | DOI: 10.53402/ajhas.v1i1.4

Abstract

The method of measuring Hb levels recommended by ICSH uses the HiCN method. However, it has a high risk due to the toxicity of the reagent. Therefore, the determination of Hb has been developed using the SLS method. According to Oshiro and Mansoor, who both stated that there was no significant difference between SLS and HiCN, this method has two distinct procedures. This study aims to determine the comparison between the measurement results of hemoglobin levels using sodium lauryl sulfate with the Oshiro procedure and Mansoor. The research design used is analytical research. The data obtained from the results of measuring Hb levels with the Oshiro and Manshoor procedures was given to students of the Diploma IV Study Program of Medical Laboratory Technology, Rajawali Health Institute batch 2018, which collected 49 people. The sampling technique used was saturated sampling to reduce the error rate in the study. The results of the normality test of the data in this study showed n of the two procedures was 49 mean±SD for the Oshiro procedure 13.09±0.56 and p = 0.200. Meanwhile, the Mansoor procedure had a meanSD of 13.09±0.57 and a p-value of 0.059. Because the probability of both being p > 0.05, the data is declared normally distributed. The results of the average difference test from the data of this study show the mean±SD of the pairwise difference between the Oshiro and Mansoor procedures is 0.002±0.059 and the p value = 0.811. If P > 0.05, then it is stated that there is no significant difference between the two groups. The conclusion of this study is that the result of measuring Hb levels using the SLS procedure is reliable. Oshiro and Mansoor conform to HiCN and there is no significant difference in the mean results.
The Effect of Different Extraction Temperatures of SARS-COV2 RT-PCR Examination Process On CT Value In Patient Specimens In Labkesda Kabupaten Sukabumi, Indonesia Fadhilah, Fitri Rahmi; Wahid, Aziz Ansori; Rachman, Mochamad Irwan Fitriana
Asian Journal of Health and Applied Sciences Vol. 1 No. 3 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i3.39

Abstract

The Severe Acute Respiratory Syndrome Coronavirus-2, or COVID, was announced by WHO on February 11, 2020.  Examination of SARS-CoV2 by RTq-PCR method in Labkesda Kab. Sukabumi is one of the health service support units in the Sukabumi area. The number of PCR examination requests is increasing every day, whereas, at this stage, the temperature increase process should reach the recommended temperature of 90°C, which can take 20-25 minutes. Sometimes ATLM has processed specimens at 60°C - 90°C. Given the required temperature at the time of extraction, which is at a temperature of 90 degrees, here the researchers wanted to prove whether below 90 degrees had an effect on the virus extraction process. This study aims to determine the effect of differences in extraction temperature on the SARS-CoV2 RTq-PCR examination process on CT VALUE on patient specimens. The variable in this study was the difference in temperature consisting of 60°C, 80°C, and 90°C. This research used quantitative research methods with case studies. This study is an experimental study to determine the effect of temperature differences on the CT value of the RTq - PCR examination. The population of this study were 30 patients at LABKESDA Kabupaten Sukabumi. This analysis used a one-way ANOVA test analysis with software. IBM SPSS 23.0. It may be concluded that there is no significant difference in the CT Value findings on the SARS-CoV2 RTq-PCR Examination since the study's p-value was 0.758, which is more than 0.05.
Molecular identification of the bacterium acute conjunctivitis by the method of sequensing gen 16S rRNA Murtafi'ah, Ni'matul; Kafesa, Ally; Wahid, Aziz Ansori
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 1 (2024): Advancements in Biomedical Research: Insights from Medical Laboratory and Technol
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i1.5207

Abstract

Conjunctivitis is the most common eye disease, characterized by contextual inflammation, which can be caused by bacteria. The diagnosis of conjunctivitis is established based various factors, including the patients’s medical history, onset of eye symptoms, non-ocular symptoms, previous disease history, family medical history, allergies, and physical examination of the eyes, which may include assessments of visual acuity and vision field. Some cases of conjunctivitis require laboratory examinations to confirm the diagnosis. These may include cytological examination with Giemsa staining, Chlamydia Diagnostic Test, Nucleic acid amplification tests (NAATs) or Polymerase chain reaction (PCR), and microbiological tests. Microbiological examination helps identify the bacteria responsible for conjunctivitis and aids in treatment by prescribing antibiotics to suppress the growth of the infecting bacteria. While identification tests are not routinely performed, researchers often seek to determine the specific type of bacteria causing conjunctivitis infections, which may require several laboratory tests. Bacterial virulence plays a significant role, with genetic mutations potentially leading to severe infections of varying severity. Virulence genes encode proteins that express pathogenic properties. The species responsible for conjunctivitis can be definitively identified definitively through microbiological examination, utilizing methods such as the 16S Ribosomal RNA Sequencing (rRNA) technique, known for its accuracy and speed. This study aims to analyze the results of rRNA sequencing in cases of acute bacterial conjunctivitis caused by 16S rRNA genotyping. The research employed an exploratory metholodolot, with the results analyzed using the The Basic Local Alignment Search Tool (BLAST) tracking program database on the National Center for Biotechnology Information (NCBI) website. The findings revealed that Sphingomonas paucimobilis encoded the 16S rRNA using Universal Primary 27 F and 1492 R, obtained in a sequence size of 1351bp. The isolate demonstrated similarities to Sphingomonas paucimobilis.
PEMANFAATAN BIOSORBEN BIJI BUAH RUDRAKSHA (Elaeocarpus Ganitrus) DALAM MENURUNKAN KADAR LOGAM BERAT CR(Vi) PADA SAMPEL AIR SUNGAI CITARUM Istiqomah, Mahmudatul; Aeni, Suci Rizki Nurul; Wahid, Aziz Ansori
Jurnal Crystal : Publikasi Penelitian Kimia dan Terapannya Vol. 7 No. 1 (2025): Publikasi Penelitian Kimia dan Terapannya 2025
Publisher : Program Studi Kimia, Fakultas MIPA, Universitas PGRI Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/jc.v7i1.5010

Abstract

Abstract Hexavalent chromium Cr(VI) is a harmful heavy metal that frequently discovered in rivers that contaminated by industrial textile waste. Biosorption is a technique that utilizes natural materials to absorb metal ions from water. This research aims to asess efficacy of biosorbent derived from rudraksha ruit seeds in diminishing Cr(VI) concentration in water samples from the Citarum River through biosorption mechanism. The biosorption was conducted by applying the Citarum River water samples with the biosorbent at varying masses of 0.1 g, 0.5 g, 1.0 g, 1.5 g, and 2.0 g. Based on the research findings, the biosorption process reduced the concentrations of Cr(VI) in the water samples from the Citarum River. The highest reduction in Cr(VI) levels occurred at a biosorbent mass of 2.0 g, with percentage reductions of 92.02% and 96.24% for water samples from Rancamanyar Village and Andir Subdistrict, respectively. Therefore, it can be inferred that the biosorbent from rudraksha fruit seeds is effective in lowering Cr(VI) levels in Citarum River water samples. Keywords: Heavy metal, Cr(VI), Biosorption, Rudraksha fruit seeds, Biosorbent Abstrak Kromium heksavalen Cr(VI) adalah logam berat berbahaya yang sering ditemukan di sungai yang terkontaminasi oleh limbah tekstil industri. Biosorpsi adalah teknik yang memanfaatkan bahan alami untuk menyerap ion logam dari air. Penelitian ini bertujuan untuk menilai efektivitas biosorben yang berasal dari biji buah rudraksha dalam mengurangi konsentrasi Cr(VI) dalam sampel air dari Sungai Citarum melalui mekanisme biosorpsi. Biosorpsi dilakukan dengan menerapkan sampel air Sungai Citarum dengan biosorben pada massa yang bervariasi yaitu 0,1 g, 0,5 g, 1,0 g, 1,5 g, dan 2,0 g. Berdasarkan temuan penelitian, proses biosorpsi mengurangi konsentrasi Cr(VI) dalam sampel air dari Sungai Citarum. Penurunan tertinggi kadar Cr(VI) terjadi pada massa biosorben 2,0 g, dengan penurunan persentase sebesar 92,02% dan 96,24% untuk sampel air dari Desa Rancamanyar dan Kecamatan Andir, masing-masing. Sehingga, dapat disimpulkan bahwa biosorben dari biji buah rudraksha efektif dalam mengurangi kadar Cr(VI) pada sampel air Sungai Citarum. Keywords: Logam berat, Cr(VI), Biosorpsi, Biji buah rudraksha, Biosorben
Co-Authors Al Badri, Muhammad Hilal Fadhila, Feldha Fadhilah, Wasiyah Khusna Fitri Rahmi Fadhilah, Fitri Rahmi Istiqomah, Mahmudatul Kafesa, Ally NI'MATUL MURTAFI'AH Rachman, Mochamad Irwan Fitriana Suci Rizki Nurul Aeni