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ANTIBACTERIAL ACTIVITY OF FENNEL ESSENTIAL OIL AGAINST PROPIONIBACTERIUM ACNES BACTERIA Wahyu Purwanjani; Jaka Lepangkari; Eka Ammyta PP
Joseph: Journal of Science and Pharmacy Vol 1 No 1 (2021): Journal of Science and Pharmacy (Joseph)
Publisher : Universitas An Nuur

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Background: Recently, natural ingredients are preferred because of their low level of toxicity to their users. Fennel has good properties as an antibacterial, so it is necessary to develop another essential oil taken from fennel to be studied for acne bacteria, namely Propionibacterium acnes. P.acnes is one of the floral bacteria on the surface of the skin, which has a role in the process of forming sebum so that acne occurs. Objective: This research was conducted to obtain the best results from the concentration of essential oils so that the essential oils with the best concentrations could be further developed in cosmetic preparations that are useful as antiacne. Method: Fennel seeds were taken and the steam detailing process was carried out for 7.5 hours. Then after the fennel essential oil was obtained, chemical compounds were tested using the Gas Chromatography-Mass Spectrophotometry (GC-MS) method to determine the chemical content of the fennel essential. Furthermore, testing the antibacterial activity of P.acnes on MHA media by inoculation of bacteria on MHA media and then the discs were immersed in different concentrations of essential oils, namely 2%, 4%, 6% and 8%. After that, compare the results of the essential oil discs with Clydamicyn positive control discs and negative control discs. Results: The identification of chemical compounds using GCMs shows that the content of several chemical constituents in essential oils is quite high, such as anetol (83.6%), Fenchone (3.58%); Linalool (0.69%); l-Limonene (3.75%); and benzaldehyde (0.41%). The antibacterial activity test of fennel essential oil against P. acnes showed a fairly large inhibitory power of 1.6 cm at a concentration of 6% fennel essential oil. Conclusion: Fennel essential oil has antibacterial activity against P.acnes bacteria so it is possible to develop it into cosmetic preparations that can inhibit the process of acne.

ANTIBACTERIAL ACTIVITY TEST OF LEAF 70% ETHANOL EXTRACT MORINGA (Moringa oleifera L.) AGAINTST BACTERIA Propionibacterium acnes Julia Sita Nur Hidayah; Supriyanto; Wahyu Purwanjani
Joseph: Journal of Science and Pharmacy Vol 2 No 1 (2022): Journal of Science and Pharmacy (Joseph)
Publisher : Universitas An Nuur

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Background: Medicinal plants contain a variety of active compounds, including as a source of antimicrobials that cause infection. The phytochemical content of Moringa leaf (Moringa oleifera) has the potential to inhibit the growth of acne-causing bacteria. The bacteria that causes acne, namely Propionibacterium acnes, produces the enzyme lipase which breaks down fat into fatty acids which causes inflammation of the skin tissue causing acne to appear. Purpose: To determine the potential of the ethanol extract of Moringa leaf in inhibiting the growth of the Propionibacterium acnes bacteria that causes acne. Method: Moringa leaf (Moringa oleifera) were prepared in powder form, the extract was obtained by maceration using 70% ethanol to obtain a thick extract. The thick extract was then identified for the chemical compounds Flavonoids, alkaloids, saponins, tannins and phenols. Then the ethanol-free test was carried out. Testing the antibacterial activity of the ethanol extract of Moringa leaf (Moringa oleifera) against P. acnes bacteria using paper disc diffusion and liquid solid dilution methods to determine the KHM and KBM values. Moringa leaf extract (Moringa oleifera) in this study used concentrations of 10%, 20% and 30%, clindamycin as a positive control and 10% DMSO as a negative control. Results: Identification of the chemical compounds of the ethanol extract of Moringa leaf showed the presence of flavonoids, alkaloids, saponins, tannins, and phenols. The ethanol-free test performed showed no ethanol content in the viscous extract. Diameter of the inhibition zone of the ethanol extract of Moringa leaf using the disc diffusion method showed the presence of optimum inhibition at a concentration of 30%. The KHM value for P. acnes bacteria starts at a concentration of 7.5%, and the KBM value at a concentration of 15%. Conclusion: The ethanol extract of Moringa leaf (Moringa oleifera L.) showed antibacterial activity against the growth of Propionibacterium acnes bacteria

FORMULASI GEL HAND SANITIZER EKSTRAK DAUN PEGAGAN (Centella asiatica) DAN AKTIVITASNYA TERHADAPStaphylococcus aureus Maulita Saraswati; Gigih Kenanga Sari; Wahyu Purwanjani
Joseph: Journal of Science and Pharmacy Vol 2 No 1 (2022): Journal of Science and Pharmacy (Joseph)
Publisher : Universitas An Nuur

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Background: Indonesia is a tropical country that has very abundant biodiversity and has various potentials for health. One of these biodiversities is pegagan leaves (Centella asiatica). Pegagan (Centella asiatica) is one of the plants that contains antibacterial substances, including flavonoids, saponins, tannins, phenols and steroids. Prevention of the spread of bacteria can be done by washing hands with soap and clean running water. Along with the development of science and technology, cleaning hands does not have to use water and soap but can use antiseptic hand gel (hand sanitizer) to kill microorganisms on the surface of the skin. Purpose: to determine whether the gotu kola leaf extract hand sanitizer gel preparation has antibacterial activity against Staphylococcus aureus Method: Extraction by maceration of pegagan leaf extract (Centella asiatica) using 96% ethanol solvent. After obtaining a thick extract, then enter it into the formulation to make a hand sanitizer gel preparation which is then tested on Staphylococcus aureus bacteria using the disc diffusion method. Results: The results of this study showed that the hand sanitizer gel with pegagan extract (Centella asiatica) showed an inhibitory effect on bacterial growth with an inhibitory value of 15.4 mm in formulation 3 with a pegagan extract concentration of 60%. Conclusion: Hand sanitizer gel from pegagan extract (Centella asiatica) has antibacterial activity against Staphylococcus aureus

Uji Stabilitas Formulasi Sediaan Gel Nano Partikel Eksktrak Etanol Daun Singkong (Manihot esculenta Crantz) Wahyu Purwanjani; Maulita Saraswati; Gigih Kenanga Sari
Joseph: Journal of Science and Pharmacy Vol 2 No 1 (2022): Journal of Science and Pharmacy (Joseph)
Publisher : Universitas An Nuur

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Background; Peel-off gel masks are an alternative dosage form that can enhance user comfort and are expected to improve the antioxidant activity of papaya fruit. Papaya (Carica papaya) exhibits antioxidant activity due to the presence of components such as beta-carotene, vitamin C, and lycopene. Objective : This study aims to determine whether the methanol extract of papaya fruit can be formulated into a peel-off facial gel mask and to identify the optimal concentration of polyvinyl alcohol (PVA)—the main base ingredient of peel-off gel masks—that provides the best physical and chemical properties, as well as antioxidant activity with the most favorable IC50 value. Method; The extraction method used was maceration with methanol as the solvent. PVA was used at concentrations of 2.5%, 8.75%, and 17.5%. Physical property tests included organoleptic evaluation, adhesion test, and spreadability test, while chemical properties were evaluated using pH testing. Results; Based on the results, the methanol extract of papaya fruit can be successfully formulated into a peel-off facial gel mask. The formula containing 8.75% PVA met organoleptic standards, had an adhesion time of 20.16 ± 2.90 seconds, spreadability of 10.2 ± 1.28 cm, a pH value of 7.13, and an IC50 value of 80.52 µg/mL, indicating strong antioxidant activity. Conclusion : From the formulation and physical evaluation of the peel-off gel mask containing ethanol extract of tea leaves (Camellia sinensis L.), a thick brown gel with a characteristic tea leaf aroma was obtained. Evaluation results showed a pH value of 5, drying time of 20 minutes, spreadability within acceptable range, adhesion time of 8.73 seconds, and no irritation was observed during the irritation test, indicating that the peel-off gel mask is safe for use

UJI AKTIVITAS SALEP EKSTRAK DAUN JAMBU AIR (Syzygiumsemarangense) DENGAN BASIS HIDROKARBON TERHADAP PENYEMBUHAN LUKA SAYAT PADA KELINCI Rismawati Suci Safitri; Supriyanto; Wahyu Purwanjani
Joseph: Journal of Science and Pharmacy Vol 3 No 1 (2023): Journal of Science and Pharmacy (Joseph)
Publisher : Universitas An Nuur

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Background: Water guava leaves contain chemical compounds in the form of alkaloids, flavonoids, saponins and tannins which are effective in healing wounds. Water guava leaves are used in several countries, including Indonesia, where they are used as a traditional medicine for healing wounds. Objective: To determine the hydrocarbon ointment base of water guava leaves (Syzygium semarangense) which can provide activity in healing cuts in rabbits. Method: Water guava leaf extract was obtained using the meseration method, then thickened and carried out a phytochemical screening test and thin layer chromatography to determine the compound content of water guava leaf extract which functions for healing cut wounds. Then it is formulated in the form of an ointment and the physical quality of the ointment is tested including organoleptic test, homogeneity, pH test, spreadability test, adhesiveness test, viscosity test, and ointment type test. Making an ointment formulation using water guava leaf extract using 3 (three) test animals with 5 (five) treatment groups, namely water guava leaf extract ointment with 10%, 15% and 20% hydrocarbon base, negative control with ointment base, control positive for povidone iodine 10%. All rabbits were injured with a wound length of 2 cm with a depth of ± 0.2 cm. The wound was smeared 3 times a day with the tested ointment. Wound observations were carried out every day (days 0 to 14). Data analysis was carried out using One-Way ANOVA.Results: The results of phytochemical screening tests and thin layer chromatography show that water guava leaves contain alkaloids, flavonoids, tannins and saponins. The formulation of water guava leaf extract ointment with a hydrocarbon base meets the physical quality test requirements for ointments such as organoleptic tests, homogeneity, pH, spreadability, stickiness, viscosity and the type of ointment produced is A/M, except for the spreadability at a concentration of 20% due to the addition The higher concentration of cera alba and vaseline causes the spreadability of the ointment to become lower. The wound narrows, forms a scab and closes the wound. The statistical test results had a significant effect on wound healing in rabbits, namely (p<0.05). Conclusion: Providing an ointment with ethanol extract of water guava leaves (Syzygium semarangense) with a hydrocarbon base at a concentration of 10% provides the most optimal effect on healing cuts in rabbits.

Uji Stabilitas Sediaan Krim Ekstrak Daun Belimbing Wuluh (Averhoa blimbi L.) dengan Variasi Konsentrasi Emulgator Maulita Saraswati; Gigih Kenanga Sari; Wahyu Purwanjani
Joseph: Journal of Science and Pharmacy Vol 3 No 1 (2023): Journal of Science and Pharmacy (Joseph)
Publisher : Universitas An Nuur

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Background: Belimbing wuluh (Averrhoa bilimbi L.) has been widely utilized as a traditional medicinal ingredient due to its antioxidant and anti-inflammatory properties. A stable cream preparation formulation is very important to maintain the effectiveness of its active ingredients. Therefore, this study aims to evaluate the physical stability of Belimbing wuluh leaf extract cream preparation with variations in emulgator concentration. Purpose: To determine the effect of varying TEA concentration on cream stability, determining the effect of variations in stearic acid concentration on cream stability, determining the optimal concentration of TEA and stearic acid to obtain a stable cream formulation. Method: This study used an experimental method with a cream formulation containing stearic acid (10%, 12%, 14%) and triethanolamine (TEA) (4%, 5%, 6%). Stability evaluation was done through organoleptic test, pH, viscosity, spreadability, adhesiveness, and cycling test. Results: The cream preparation showed good homogeneity with a light brown color and characteristic odor of the extract. The pH value ranged from 7.50 to 8.04, in accordance with topical cream standards. Viscosity increased as the concentration of stearic acid and TEA increased. Spreadability and stickiness decreased after the accelerated stability test, but remained within acceptable limits. Conclusion: Cream formulations with a combination of stearic acid and TEA provide good physical stability. The formula with 14% stearic acid and 6% TEA showed more optimal physical characteristics than the other formulas

Uji Aktivitas Serum Anti Aging Ekstrak Daun Salam (Syzygium polyanthum) Wahyu Purwanjani
Joseph: Journal of Science and Pharmacy Vol 3 No 1 (2023): Journal of Science and Pharmacy (Joseph)
Publisher : Universitas An Nuur

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Background : Skin aging is a natural process characterized by a decrease in skin elasticity, firmness, and moisture, as well as the appearance of wrinkles caused by both intrinsic and extrinsic factors such as UV exposure and free radicals. The use of natural ingredients as anti-aging agents is increasingly being explored, one of which is bay leaf (Syzygium polyanthum), known to contain active compounds such as flavonoids, tannins, and antioxidants that can combat free radicals and slow down the skin aging process. Objective : To evaluate the anti-aging activity of a serum formulation containing bay leaf extract as the main ingredient. Method : Bay leaf extract was obtained through a maceration process and then formulated into a serum with various concentrations. The effectiveness test was conducted on rabbits using a Skin Analyzer device to measure skin parameters such as moisture, elasticity, and wrinkle depth before and after serum application. Results : The study showed that the serum containing 4.5% extract concentration provided the most significant improvement in skin condition, with notable increases in moisture and elasticity and a marked reduction in wrinkle depth. Therefore, bay leaf extract has potential as an active agent in anti-aging skincare products.

UJI AKTIVITAS ANTIBAKTERI SEDIAAN FACE TONER EKSTRAK ETANOL 70% DAUN SALAM (Syzygium polyanthum) TERHADAP BAKTERIStaphylococcus aureus Sony Hartono; Wahyu Purwanjani; Maulita Saraswati
Joseph: Journal of Science and Pharmacy Vol 4 No 1 (2024): Journal of Science and Pharmacy (Joseph)
Publisher : Universitas An Nuur

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Background: Salam leaves contain several chemical compounds including alkaloids, flavonoids, saponins, triterpenoids and tannins which are active compounds that are effective as drugs that can cure infectious diseases caused by bacteria. Purpose: The purpose of this study was to determine the antibacterial activity of bay leaf extract face toner preparations against Staphylococcus aureus bacteria. Method: Bay leaves are extracted with 70% ethanol by maceration. The ethanol extract of bay leaves is formulated into a facial toner preparation and then tested for antibacterial activity using the disc diffusion method or the Kirby Bauer method. Results: The results of the research show that the face toner preparation with 70% ethanol extract of salam (Syzygium polyanthum) contains alkaloid compounds, flavonoids, saponins, triterpenoids and tannins. Ethanol extract of salam leaf has antibacterial activity against Staphylococcus aureus with the inhibition zone diameter of F1 of 10.28 mm, F2 of 11.24 mm, and F3 of 12.24 mm. Conclusion: Facial toner preparation of bay leaf extract (Syzygium polyanthum) provides the strongest antibacterial activity in F3 against the growth of Staphylococcus aureus bacteria with an inhibitory zone diameter of 12.24 mm

UJI AKTIVITAS ANTI JAMUR SEDIAAN GEL EKSTRAK DAUN TURI PUTIH (Sesbania grandiflora L.) TERHADAP JAMUR Candida albicans Sinta ardani; Supriyanto; Wahyu Purwanjani
Joseph: Journal of Science and Pharmacy Vol 4 No 1 (2024): Journal of Science and Pharmacy (Joseph)
Publisher : Universitas An Nuur

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Background: One of the plants that has anti-fungal potential is the white Turi Plant (Sesbania grandriflora) white turi leaf extract (Sesbania grandiflora) which is suspected to be able to inhibit the growth of Candida albicans, one of the fungi that cause candidiasis. Purpose: This study aims to determine the antifungal activity of Sesbania grandriflora white turi leaf extract gel against the fungus Candida albicans, determine the minimum inhibitory concentration (KHM) and determine the content of secondary metabolite compounds. Method: The leaves (Sesbania grandriflora) are extracted by maceration with a 70% ethanol solvent. The extracts obtained were subjected to phytochemical screening tests. The antifungal activity test of turi leaf extract gel was carried out by the well method against Candida albicans. The positive control used was the 2% dandruff gel while the negative control used was the gel preparation without the extract. Results: White turi leaf extract gel (Sesbania grandriflora) has antifungal activity against Candida albicans. Conclusion: Based on the results of the research on the Antifungal Activity Test of Sesbania glandriflora White Turi Leaf Extract Gel Preparation against Candida albicans Fungus, it can be concluded as follows: 1. Sesbania glandriflora (L.) white turquoise leaf extracthas the content of Saponins, flavonoids, and tannins 2. Sesbania glandriflora (L.) white turquoise leaf extractgel preparation has the activity of inhibiting the fungusCandida albicans The most effective formulation of Sesbania glandriflora (L.) white turi extract gel preparation against the growth of Candida albicans fungus is Formulation 3 with a concentration of 8% of 20.65 mm, included in the very strong group

Analisis Zat Pengawet Boraks pada Cilok dan Pentol Bakso yang Dijual di Kabupaten Grobogan Salma Ellya Sayidina; Wahyu Purwanjani; Estuningtyas Ayu Hapsari
Joseph: Journal of Science and Pharmacy Vol 5 No 1 (2025): Journal of Science and Pharmacy (Joseph)
Publisher : Universitas An Nuur

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Background: The increasing use of borax as an unauthorized preservative in foodssuch as cilok and pentol bakso sold in Grobogan Regency poses a threat to public health. Purpose: to examine the presence and concentration of borax in cilok and pentol bakso samples circulating in the area. Method: Qualitative analysis using boric acid test kits and quantitative analysis using Spectrophotometry UV-Vis to measure boric acid levels in the samples. Samples were collected from various street vendors, prepared and then analyzed in the laboratory. Results: The study results showed that borax was detected in one cilok sample with a the highest concentration reaching 112.22 μg/kg. Conclusion: The study results indicate that some food vendors are still adding borax to their products. Therefore, further supervision and increased awareness regarding the risks of using borax in food are necessary.

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