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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Biosense International Journal of Health Science and Technology Bincang Sains dan Teknologi
Sharfina Mutia Syarifah
Universitas Aisyiyah Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Electrical & Electronics Engineering Energy Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Transportation

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UJI ANTAGONIS Bacillus megaterium TERHADAP Fusarium oxysporum DAN PENGARUHNYA PADA PERTUMBUHAN TANAMAN CABAI RAWIT Ika Afifah Nugraheni; Nita Widyaningsih; Sharfina Mutia Syarifah; Wisnu Adhi Susila
JURNAL BIOSENSE Vol 5 No 01 (2022): Edisi Juni 2022
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.319 KB) | DOI: 10.36526/biosense.v5i01.1877

Abstract

Bacillus megaterium merupakan jenis bakteri endofit yang mempunyai potensi untuk dikembangkan sebagai bahan baku pupuk hayati. Selain mampu memaksimalkan penyerapan nutrisi tanaman, B. megaterium juga dapat menghambat pertumbuhan patogen tanaman, termasuk pada tanaman cabai. Tanaman cabai (Capsicum frutescens) masih dianggap sebagai komoditas yang menguntungkan, di samping karena permintaannya yang terus meningkat setiap tahun. Oleh karena itu, penelitian ini bertujuan untuk menguji aktivitas antagonis B. megaterium terhadap Fusarium oxysporum dan mengamati pengaruhnya pada pertumbuhan tanaman cabai rawit. Metode yang digunakan yaitu uji antagonis dengan teknik dual culture, dan pengaplikasian B. megaterium ke tanaman cabai rawit dengan variasi dosis, meliputi K, A1, A2, A3 dan A4. Berdasarkan penelitian, B. megaterium mampu menunjukkan penghambatan terhadap F. oxysprorum sebesar 67% pada hari ke sepuluh. Selain itu, tanaman cabai rawit yang diaplikasikan B. megaterium memberikan pertumbuhan yang baik dibandingkan kontrol untuk variabel tinggi tanaman, jumlah daun, Panjang akar, berat segar dan berat kering tanaman. Dari variasi dosis yang diberikan, perlakuan A4 dengan dosis 25 mL mampu memberikan pertumbuhan terbaik. Hal ini menunjukkan bahwa B. megaterium bersifat antagonis terhadap F. oxysporum dan mampu meningkatkan pertumbuhan tanaman cabai rawit.
Pengurai Sampah Plastik Ramah Lingkungan Nurul Abidin; Wahdaniar; Novi Febrianti; Sharfina Mutia Syarifah
Bincang Sains dan Teknologi Vol. 2 No. 02 (2023): Bincang Sains dan Teknologi
Publisher : The Indonesian Institute of Science and Technology Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56741/bst.v2i02.339

Abstract

Tujuan dari penelitian ini adalah untuk menyelidiki mekanisme penguraian sampah plastik oleh berbagai mikroorganisme, termasuk bakteri, cendawan, dan ulat. Hasil penelitian menunjukkan bahwa mekanisme penguraian plastik melibatkan berbagai strategi biokimia dan enzimatik yang berbeda pada setiap mikroorganisme. Bakteri seperti Ideonella sakaiensis 201-F6 memiliki enzim PETase yang mampu mengurai plastik PET menjadi senyawa monomer yang lebih sederhana. Cendawan seperti Trichoderma viride dan Aspergillus nomius juga memiliki enzim ekstraseluler dan kemampuan untuk mengurai berbagai jenis plastik. Sementara itu, ulat Galleria mellonella dan larva Tenebrio molitor menggunakan mekanisme pencernaan yang melibatkan enzim dan kerjasama dengan bakteri dalam sistem pencernaan mereka. Ulat G. mellonella memakan plastik polyethylene (PE) yang mirip dengan struktur karbon lilin lebah yang menjadi sumber makanan alaminya, sementara larva T. molitor memakan plastik polystyrene (PS) dan menghasilkan enzim serta bakteri dalam sistem pencernaannya untuk menguraikan styrofoam menjadi senyawa organik yang lebih sederhana. Mekanisme penguraian sampah plastik oleh mikroorganisme masih dalam tahap penelitian yang terus berkembang, dan masih banyak hal yang perlu dipahami dengan lebih mendalam. Penelitian lebih lanjut diperlukan untuk mengidentifikasi enzim, jalur metabolic, dan mekanisme detil lainnya yang terlibat dalam penguraian sampah plastik oleh mikroorganisme. Penemuan lebih lanjut tentang mekanisme ini dapat berpotensi menjadi sumber inspirasi untuk pengembangan teknologi bioteknologi yang dapat membantu mengurangi masalah sampah plastik.
The effect of Gandasil and LB fertilizer concentrations on the growth of Dendrobium sp Syarifah, Sharfina Mutia; Marsuni, Jenni Fer Try; Fardhani, Dinar Mindrati; Tryana, Elys
International Journal of Health Science and Technology Vol. 5 No. 3 (2024): April
Publisher : Universitas 'Aisyiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31101/ijhst.v5i3.3306

Abstract

Dendrobium sp. orchids, especially hybrid varieties, are one of the species that can tolerate direct sunlight well. Fertilizing these orchids involves using foliar fertilizers, made from organic or chemical materials, applied through the leaves' stomata. This method involves spraying the fertilizer to provide additional nutrients beyond what the roots absorb. This research aimed to determine the effects of fertilizers on leaf count and plant height. The experiment included two treatments and two replications, with methods such as media sterilization, sample preparation, watering, transplanting, fertilizing, and plant observation. Data were analyzed using one-way ANOVA with a p-value <0.05. Foliar fertilizer was applied to the underside of the leaves between 8-9 AM or 3-4 PM when stomata were open. Spraying was avoided during the rainy season to prevent fertilizer runoff while stomata were closed. Observations revealed that using Grow Quick Leaf Booster (LB) fertilizer at a concentration of 2 ml (diluted with ½ water) promoted longer growth than Gandasil fertilizer at 1 ml (diluted with ½ L of water and 1 tablespoon), which also produced good results and accelerated orchid growth. Gandasil increased plant height, leaf count, leaf diameter, and the number of seedlings, while LB fertilizer primarily enhanced plant height. These findings suggest that while both fertilizers improve growth, Gandasil has a broader range of benefits, including vegetative growth and leaf development, whereas LB is more focused on increasing plant height.
Pengendalian Hayati Patogen Fusarium oxysporum f. sp. capsici dengan Isolat Trichoderma sp. Asal Rizosfer Bambu dari Kecamatan Kedu, Kabupaten Temanggung Syarifah, Sharfina Mutia; Sari, Ofivah Permata; Bimantara, Arif
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 3 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.3.454

Abstract

Wilt disease caused by Fusarium oxysporum f. sp. Capsici can cause serious problems and reduce red chili yields, so controls are needed that can reduce the development of the pathogen. The purpose of this study was to determine the potential of Trichoderma sp. from the bamboo rhizosphere in Kedu District, Temanggung Regency, in inhibiting the growth of F. oxysporum f. sp. capsici in vitro and in vivo. Trichoderma sp. Was isolated from the rhizosphere of bamboo plants, and F. oxysporum f. sp. capsici was isolated from red chili plants with symptoms of fusarium wilt. Mushroom isolates were purified and identified based on the identification key book. An in vitro test using the double culture method and an in vivo test were conducted in a screen house by applying Trichoderma sp. to the stem of red chili plants. One isolate of Trichoderma sp. was obtained from the soil of the bamboo rhizosphere in the Kedu District, and Fusarium sp. isolates were obtained from the stems of red pepper plants. Based on in vitro antagonist tests, Trichoderma sp. Fungi were able to inhibit the growth of F. Oxysporum f. sp. capsici up to 82.50% at 7 HSI. Trichoderma sp. could inhibit pathogens through antibiotic inhibition mechanisms and competition of nutrients and space to grow. Trichoderma sp. inoculation followed by in vivo inoculation of pathogens on red chili plant stems showed an attack symptom intensity of 4.16% with an antagonistic performance of 75.03% 12 days after inoculation. Trichoderma sp. is effective as an alternative biological control agent for red chili plant disease caused by F. Oxysporum f. sp. capsici. Keywords: Bamboo Rhizosphere, Fusarium oxysporum f. sp. capsici, Plant Diseases, Trichoderma sp.
Co-Authors Arif Bimantara Dinar Mindrati Fardhani Ika Afifah Nugraheni Marsuni, Jenni Fer Try Nita Widyaningsih Novi Febrianti Nurul Abidin Sari, Ofivah Permata Tryana, Elys W, Wahdaniar Wisnu Adhi Susila