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All Journal Vegetalika Jurnal Penelitian Pendidikan IPA (JPPIPA)
Wahyu Indra Duwi Fanata
University of Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Peningkatan Aktivitas Enzim Metabolisme Sukrosa dan Glikolisis pada Tanaman Padi Transgenik Overekspresi Gen SoSPS1 Ferdi Hariyanto; Bambang Sugiharto; Netty Ermawati; Wahyu Indra Duwi Fanata
Vegetalika Vol 15, No 2 (2026): In Publish
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.114798

Abstract

Sucrose Phosphate Synthase (SPS) is a key enzyme in the sucrose biosynthesis pathway and plays an essential role in regulating sucrose accumulation in plants. Overexpression of the SPS gene has been reported to enhance sucrose accumulation and growth in various plant species. However, its effects on the dynamics of sucrose-metabolising and glycolytic enzymes in rice remain insufficiently explored. This study aimed to analyze the activities of sucrose metabolism enzymes, including SPS, invertases (acid and neutral), sucrose synthase (SuSy), and the glycolytic enzyme hexokinase (HXK) in transgenic rice plants overexpressing the SoSPS1 gene. The experiment was conducted using a completely randomized design with five genotypes, one of which was the wild type, and four transgenic rice lines, T4, T6, T8, and T9, with four replicates. The results showed that overexpression of the SoSPS1 gene led to significant increases in plant height and biomass, as well as markedly enhanced SPS activity in all transgenic rice plants compared to the wild type. Invertase activity (A-INV and N-INV) did not differ significantly among genotypes. In contrast, SuSy activity increased dramatically in transgenic lines T6, T8, and T9, suggesting that sucrose degradation in response to the genetic modification is primarily regulated through SuSy. The increase in SuSy activity was accompanied by the rise in HXK activity in the same lines (T6, T8, and T9). These results indicate that HXK subsequently phosphorylate hexose sugars, which may act as substrates for cellulose synthesis and activate the transcription factors that lead to higher biomass production. Overall, this study demonstrates that overexpression of SoSPS1 influences carbon flux and sugar metabolism, providing deeper insights into the relationship among sucrose biosynthesis, sugar sensing, and biomass enhancement in transgenic rice.
KARAKTERISASI SENYAWA AKTIF PADI LOKAL INDONESIA TAHAN TERHADAP CEKAMAN LINGKUNGAN Tri Ratnasari; Wahyu Indra Duwi Fanata; Ummi Sholikhah; Ahmad Ilham Tanzil
Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9iSpecialIssue.5644

Abstract

The purpose of this study was to characterize the active compounds in Indonesian local rice that are resistant to environmental stress in order to identify the nature of resistance to environmental stress, one of the indicators of which is the presence of flavonoid compounds. This step is one of the efforts that will lead to the acquisition of gene donors in the assembly of varieties resistant to biotic stress (pests and diseases) and abiotic stress (drought, high salinity, low temperature), which are very necessary in rice breeding programs. The method used is to plant 8 local rice varieties that are resistant to environmental stress in buckets in greenhouses. Next, analyze the content of active compounds by extracting the leaves and stems of rice plants, then macerate with methanol for 48 hours and test the content of active compounds. Then the total flavonoid content test was carried out using a quercetin standard and measured using a spectrophotometer. Whatever the results of this study, all samples showed active compounds containing flavonoids, alkaloids, tannins and saponins which were indicated by indicators of color change when tested with certain reagents. The results of the Flavonoid content analysis showed that the pendok variety had the highest flavonoid content, namely 3.9253 ± 0.06, and the Sigupai variety had the lowest flavonoid content, with a value of 1.8073 ± 0.07.
Co-Authors Ahmad Ilham Tanzil Bambang Sugiharto Ferdi Hariyanto Netty Ermawati Tri Ratnasari Ummi Sholikhah