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All Journal AL KAUNIYAH BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Pengabdian Magister Pendidikan IPA Agroradix : Jurnal Ilmu Pertanian
Tri Ratnasari
Universitas Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Materials Science & Nanotechnology Physics

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THE FLAVONOID AND ALKALOID CONTENT OF CYCLOSORUS PARASITICUS (LINN.) FARWELL FERNS AT THE PLANTATION AREAS OF JEMBER REGENCY Dwi Setyati; Hari Sulistiyowati; Monica Paulina Erizcy; Tri Ratnasari
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.028 KB) | DOI: 10.31289/biolink.v7i1.3026

Abstract

Cyclosorusparasiticus(Linn.) Farwell is one potential medicinal ferns. The plant contains secondary metabolites, such as flavonoids and alkaloids. This study aims to determine the flavonoid and alkanoidofCyclosorusparasiticus (Linn.) Farwell in three plantation areas of  Jember Regency. Plant samples were collected from Mount Gumitir coffee plantations, Pine Garahan Village and rubber and cocoa plantations in Tancak in Jember. Samples of stems  and leaves were dried at room temperature and then blended to obtain a powder. One gram of powder samples was macerated in 90 ml of methanol for 3x24 hours then was concentrated with an evaporator to obtain a crude extract. The crude extract was tested qualitatively for flavonoids and alkaloids by the Willstätter and Dragendorff methods followed by quantitatively tests with Spectrophotometric. The results showed thatboth flavonoids and alkaloids were found in stem and leavesof Cyclosorusparasiticus (Linn) Farwell growing at three research locations. The flavonoids and alkaloidsin content found in leaveswas higher than those in stem organs. The leaves of Cyclosorusparasiticus (Linn) Farwell in Gumitir contain the highest flavonoids and alkaloidscontentsthan those in Tancak.
PENGARUH SAMPAH ORGANIK TERHADAP KARAKTERISTIK KIMIA VERMIKOMPOS Ahmad Ilham Tanzil; Puji Rahayu; Raudhotun Jamila; Wahyu Indra Duwi Fanata; Ummi Sholikhah; Tri Ratnasari
AGRORADIX : Jurnal Ilmu Pertanian Vol 7 No 1 (2023): Desember 2023
Publisher : Agroteknologi Fakultas Pertanian Universitas Islam Darul 'Ulum (UNISDA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/agroteknologi.v7i1.5262

Abstract

Vermicompost is a fertilizer produced by composting organic matter by utilizing the interaction between earthworms and microorganism. Vermicompost also contains a number of macro and micro nutrients needed by plants. Besides containing macro and micro of nutrients, vermicompost also containing growth hormone such as cytokinins and auxins which are produced by worms during the composting process. The benefits of vermicompost are that it can improve the physical, chemical and biological properties of soil, increase the ability of the soil to retain water, rebuild damaged soil structures and increase ability of the soil to exchange cations. The fermentation process lasted for 14 days using Lumbricus rubellus worms. The choice of this type worm is based on its character which has a high level of productivity, can eat all types of organic matter, is much more adaptive and move less actively. This research was held in Sumbersalak Village, Ledokombo District, Jember Regency and also involving the Kenconowungu farmer group. Goat manure become waste in this village so it has potential to be developed further as the main ingredient for vermicompost. The other additional materials such as vegetable and fruits waste, and leaf litter were used as factor (treatment). These three types of materials are used to find out which treatment gives the best vermicompost quality. Chemical analysis was carried out in this study to determine the organic N, P, K, C organic, pH, and water content. The result of the analysis will be compared with the standards of Minister of Agriculture No.70 of 2011.
Respon IAA dan BAP Terhadap Multiplikasi In Vitro Tunas Tanaman Krisan Varietas Suciono (Chrysanthemum indicum L) Didik Pudji Restanto; Nur Lailin Nafiah; Wahyu Indra Duwi Fanata; Tri Ratnasari; Refa Firgiyanto
Al-Kauniyah: Jurnal Biologi Vol 17, No 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.1.27417

Abstract

AbstrakTanaman krisan varietas Suciono merupakan tanaman hias yang potensial dikembangkan dan diminati oleh penduduk dunia, yang ditunjukkan dengan permintaan pasar terhadap tanaman krisan meningkat sepanjang tahun. Hal ini menyebabkan perbanyakan krisan secara in vitro sangat penting untuk dilakukan. Penelitian ini bertujuan untuk mengetahui pengaruh Indole Acetic Acid (IAA) dan Benzly Amino Purine (BAP) terhadap multiplikasi tunas krisan varietas Suciono. Desain penelitian ini menggunakan Rancangan Acak Lengkap dengan satu faktor yakni kombinasi hormon IAA dan BAP yang 9 kombinasi, yakni 0 mg/L +  1,5 mg/L; 0 mg/L + 2 mg/L; 0 mg/L + 2,5 mg/L; 0,5 mg/L + 1,5 mg/L; 0,5 mg/L + 2 mg/L; 0,5 mg/L + 2,5 mg/L; 1 mg/L + 1,5 mg/L; 1 mg/L + 2 mg/L; dan 1 mg/L + 2,5 mg/L dimana setiap kombinasi diulang sebanyak 3 kali. Variabel yang diamati meliputi analisis histologis kalus, waktu muncul tunas, jumlah tunas, tinggi tunas, dan jumlah daun. Hasil analisis ragam atau ANOVA menunjukkan bahwa kombinasi IAA 0 mg/L dan BAP 2 mg/L merupakan kombinasi konsentrasi terbaik untuk multiplikasi tunas. Penambahan BAP 2 mg/L mampu menghasilkan persentase muncul tunas. 100%, jumlah tunas 3 per-eksplan, tinggi tunas 7 cm, dan jumlah daun sebanyak 16 helai.AbstractThe variety Suciono of chrysanthemum is a potential ornamental plant that is in demand by the world's population, as indicated by the increasing market demand for chrysanthemum plants throughout the year. This  has led to the importance of in vitro propagation of chrysanthemum. The aim of the this study is to determine the effect of Indole Acetic Acid (IAA) and Benzly Amino Purine (BAP) on the multiplication of chrysanthemum shoots of the Suciono variety. The design of this study used nodus explants, treated with a combination of IAA hormones and BAP. It is make 9 combinations, and then the combinations of hormones is 0 mg/L + 1,5 mg/L; 0 mg/L + 2 mg/L; 0 mg/L + 2,5 mg/L; 0,5 mg/L + 1,5 mg/L; 0,5 mg/L + 2 mg/L; 0,5 mg/L + 2,5 mg/L; 1 mg/L + 1,5 mg/L; 1 mg/L + 2 mg/L; and 1 mg/L + 2,5 mg/L.  The observed variables included histological analysis of callus, time of shoot emergence,  number of shoots, shoots height, and number of leaves. The results of the analysis showed that the treatment with 0 mg/L IAA and 2 mg/L BAP was the best concentration for shoots multiplication. The addition of 2 mg/L BAP was able to produce a percentage of shoots emergence of 100%, 3 shoots per explant, shoots height of 7 cm and 16 leaves.
Pendampingan Pemanfaatan Larutan KNO3 terhadap Perkecambahan dan Pertumbuhan Awal Benih Padi di Desa Sumbersalak Kecamatan Ledokombo Kabupaten Jember Adin Novitasari; Sri Rejeki Utami; Aminatun Nisa; Ummi Sholikah; Ahmad Ilham Tanzil; Wahyu Indra Duwi Fanata; Tri Ratnasari; Yusuf Rachmandika
Jurnal Pengabdian Magister Pendidikan IPA Vol 9 No 1 (2026): Januari - Maret 2026
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v9i1.14442

Abstract

Farmers in Sumbersalak Village, Ledokombo, Jember Regency still depend on rice as the main commodity in their business. One important component that supports the success of rice cultivation is the availability of quality seeds. One obstacle faced in rice seed production is dormancy. One effort to improve the quality of seeds that have been stored for a long time is to give rice seeds special treatment in the hope of improving germination and early growth. The method used is participatory assistance, including dissemination of information, discussion, and direct practice of seed soaking. The activity was carried out in July 2025 at the Kenconowungu Farmer Group, involving 10 participants. The rice seeds were soaked in a 3% KNO3 solution for 24 hours. The results showed that the KNO3 treatment was effective as an invigoration technique that improved the physiological characteristics of the seeds, which had deteriorated during storage. The application of this technology was proven to increase the germination rate of local rice seeds to be faster and more uniform compared to those without treatment. Additionally, this assistance successfully improved farmers' understanding and independence in managing seeds in a practical and applicable manner.
Morpho-Physiological Responses of Purbalingga and Purowkerto Local Black Rices to Drought Stress Ummi Sholikhah; Khusna Khamidah; Tri Handoyo; Ahmad Ilham Tanzil; Wahyu Indra Duwi Fanata; Tri Ratnasari; Tri Wahyu Saputra
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 4 (2025): August 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v14i4.1148-1155

Abstract

Black rice (Oryza sativa L. indica) is a pigmented rice plant that has high antioxidant content. Drought is an abiotic stress that can inhibit the growth and productivity of rice plants. Planting of local black rice with several drought treatments using PEG 6000 was observed in this study. The aim of this research is to determine the morphological and physiological response of local black rice plants in Purbalingga and Purwokerto at various levels of drought stress. This research used a Completely Randomized Design (CRD) with 2 factors. The first factor is 2 local black rice plants, namely Purbalingga and Purwokerto. The second factor was drought stress treatment via PEG 6000 with 4 treatments, namely 0% PEG (control), 5%, 10%, and 15%. The results showed that when applying 15% PEG, local black rice in Purbalingga and Purwokerto experienced a decrease in plant height and number of leaves but increased root length. Based on the physiological response, when addition 15% PEG there was a decrease in the amount of chlorophyll and an increase in the content of H2O2 and anthocyanins in both Purbalingga and Purwokerto local black rice.   Keywords: Black Rice, Drought Stress, Morphological Response, Physiological Response.
Growth and Yield of Vertical Hydroponic Lettuce under Liquid Organic Fertilizer and Shade Intensity Treatments Ika Purnamasari; Tri Ratnasari; Berlian Cindy Febrian; Annisa Lailatus Sufiah; Bintang Kurniawan; Ummi Sholikhah; Tri Wahyu Saputra; Wildan Muhlison
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 15 No. 2 (2026): April 2026
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v15i2.689-701

Abstract

Lettuce (Lactuca sativa L.) production needs to be increased to fulfill increasing demand. Application of liquid organic fertilizer (LOF) in the hydroponic nutrient solution and adding shade to reduce solar radiation intensity are expected to improve lettuce yield. The purpose of this study was to investigate the effect of providing shade and adding LOF as a nutrient to optimize the results of hydroponic lettuce cultivation in Situbondo Regency. This study used a factorial Randomized Block Design (RBD) with UV plastic shading as a factor at different levels, namely 6% and 14%. The second factor was the nutrient composition used, namely 100% AB mix, 25% LOF + 75% AB mix, and 50% LOF + 50% AB mix. The results showed that the interaction of the two factors showed a significant difference in the chlorophyll content of plant leaves. The application of each factor was able to influence plant height, number of leaves, fresh weight, leaf area, leaf color, and crispness level, but did not affect the canopy area of lettuce plants. The use of 14% UV plastic and the addition of 25 LOF, separately resulted the highest yield of lettuce.
KARAKTERISASI SENYAWA AKTIF PADI LOKAL INDONESIA TAHAN TERHADAP CEKAMAN LINGKUNGAN Tri Ratnasari; Wahyu Indra Duwi Fanata; Ummi Sholikhah; Ahmad Ilham Tanzil
Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9iSpecialIssue.5644

Abstract

The purpose of this study was to characterize the active compounds in Indonesian local rice that are resistant to environmental stress in order to identify the nature of resistance to environmental stress, one of the indicators of which is the presence of flavonoid compounds. This step is one of the efforts that will lead to the acquisition of gene donors in the assembly of varieties resistant to biotic stress (pests and diseases) and abiotic stress (drought, high salinity, low temperature), which are very necessary in rice breeding programs. The method used is to plant 8 local rice varieties that are resistant to environmental stress in buckets in greenhouses. Next, analyze the content of active compounds by extracting the leaves and stems of rice plants, then macerate with methanol for 48 hours and test the content of active compounds. Then the total flavonoid content test was carried out using a quercetin standard and measured using a spectrophotometer. Whatever the results of this study, all samples showed active compounds containing flavonoids, alkaloids, tannins and saponins which were indicated by indicators of color change when tested with certain reagents. The results of the Flavonoid content analysis showed that the pendok variety had the highest flavonoid content, namely 3.9253 ± 0.06, and the Sigupai variety had the lowest flavonoid content, with a value of 1.8073 ± 0.07.
Analysis of the Application of Vitamin B1 on the Response of Salinity Stress Resistance in Several Varieties of Rice (Oryza Sativa L.) Tri Ratnasari; Tri Handoyo; Parawita Dewanti; Didik Pudji Restanto
Jurnal Penelitian Pendidikan IPA Vol 10 No 8 (2024): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i8.6324

Abstract

One of the most common stresses in rice cultivation is salinity. Rice plants stressed by salinity exhibit changes such as yellowing leaves, drying tips, and chlorosis. The efforts made by the government and farmers so far include implementing cultivation scheduling techniques, planting patterns, and using stress-resistant varieties, as well as improving soil to increase water-holding capacity through lime application. Each of these efforts comes with its own risks. Another approach to enhance the growth and yield of rice plants is the application of vitamins. Providing vitamins can stimulate the growth of plant organs, as they play a crucial role in the growth process by acting as catalysts for metabolism. Research has indicated that vitamin B1 can significantly promote plant growth under stressful conditions. This study aims to investigate the positive effects of various concentrations of vitamin B1 on the growth and yield of rice plants while also reducing salinity stress. The method used involved planting three varieties of rice—IR-46, Inpari-32, and Pokkali—in planting buckets using the TABELA system. Vitamin B1 was applied at concentrations of 0, 5, and 10 mM during the peak vegetative phase, with salinity stress of 6 dS/m introduced one day after vitamin application. The plants were maintained under salinity stress conditions until harvest, during which morphological and phytochemical analyses were conducted. Morphological analysis included measurements of plant height, number of tillers, number of productive tillers, number of grains per panicle, and percentage of healthy grains. Biochemical parameters measured included total chlorophyll and electrolyte leakage analysis. The results indicate that vitamin B1 can effectively reduce stress in plants affected by salinity.
Co-Authors Adin Novitasari Ahmad Ilham Tanzil Ahmad Ilham Tanzil Aminatun Nisa Annisa Lailatus Sufiah Berlian Cindy Febrian Bintang Kurniawan Dwi Setyati Ika Purnamasari Khusna Khamidah Monica Paulina Erizcy Nur Lailin Nafiah Parawita Dewanti Puji Rahayu Raudhotun Jamila Refa Firgiyanto Restanto, Didik Pudji Sri Rejeki Utami Sulistiyowati, Hari TRI HANDOYO Tri Wahyu Saputra Ummi Sholikah Ummi Sholikhah Ummi Sholikhah Wahyu Indra Duwi Fanata Wahyu Indra Duwi Fanata Wildan Muhlison, Wildan Yusuf Rachmandika