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All Journal Journal of Noncommunicable Diseases (JOND) Jurnal Katalisator
Imrawati Imrawati
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Agriculture, Biological Sciences & Forestry Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Identifikasi Kandungan Senyawa dan Potensi Ekstrak Etanol 96% Daun Tekelan (Chromolaena odorata L.) sebagai Antioksidan Penangkal Radikal ABTS Yuri Pratiwi Utami; Imrawati Imrawati; Astuti Amin; Fadel Ahmad Haris
JOURNAL OF NONCOMMUNICABLE DISEASES Vol 3, No 2 (2023): November 2023
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jond.v3i2.785

Abstract

Tekelan (Choromolaena odorata L.) merupakan tanaman yang mengandung senyawa yang dapat berperan sebagai antioksidan. Tujuan penelitian ini dilakukan untuk identifikasi kandungan senyawa dan mengetahui potensi ekstrak etanol 96% daun tekelan menggunakan metode ABTS. Simplisia yang diperoleh selanjutnya diekstraksi dengan metode maserasi menggunakan etanol 96%, dihitung rendemennya dan diidentifikasi kandungan senyawa menggunakan metode KLT kemudian dilakukan uji aktivitas antioksidan dengan metode ABTS. Diperoleh hasil penelitian yaitu kadar air simplisia 9%, rendemen ekstrak 12,4%, kandungan senyawa dalam ekstrak etanol daun tekelan yaitu alkaloid, flavonoid, steroid dan tannin menggunakan metode KLT. Nilai IC50 ekstrak yaitu 28,323 μg/mL menunjukkan aktivitasnya sebagai antioksidan dan pembanding kuersetin dengan nilai IC50 2,334 μg/mL. Dapat disimpulkan potensi antioksidan ekstrak etanol 96% daun tekelan memiliki aktivitas antioksidan yang sangat kuat, hal ini dikarenakan adanya kandungan senyawa flavonoid yang memiliki potensi sebagai antioksidan. Tekelan (Choromolaena odorata L.) is a plant that contains compounds that can act as antioxidants. The aim of this research was to identify the compound content and determine the potential of a 96% ethanol extract of tekelan leaves using the ABTS method. The simplicia obtained was then extracted using the maceration method using 96% ethanol; the yield was calculated; the compound content was identified using the TLC method; and antioxidant activity was tested using the ABTS method. The results obtained were that the water content of simplicia was 9%, the extract yield was 12.4%, and the compound content in the ethanol extract of tekelan leaves was alkaloids, flavonoids, steroids, and tannins using the TLC method. The IC50 value of the extract is 28.323 μg/mL, indicating its activity as an antioxidant and a comparison for quercetin with an IC50 value of 2.334 μg/mL. It can be concluded that the antioxidant potential of the 96% ethanol extract of tekelan leaves has very strong antioxidant activity. This is due to the content of flavonoid compounds, whichhave potential as antioxidants.
Α-GLUCOSIDASE ENZYME INHIBITOR ACTIVITY OF ETLINGERA ELATIOR LEAF EXTRACT WITH SOLVENT VARIATION Imrawati Imrawati; Yuri Pratiwi Utami; Yuniar Dwi Yanti; Tuti Handayani; Nurzadrina wahyuddin; Zahira Amody; Aulia Debby Pelu; Rahmah Mustarin; Indri Indri
JURNAL KATALISATOR Vol. 10 No. 2 (2025): Jurnal Katalisator, Volume 10 No 2 Oktober 2025
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v10i2.3462

Abstract

Diabetes mellitus (DM) is a chronic metabolic disease characterized by hyperglycemia due to impaired insulin production or utilization. The increasing prevalence of diabetes, especially type 2, is a global concern due to the risk of complications it poses. One approach in treating type 2 diabetes is to inhibit the α-glucosidase enzyme to slow down glucose absorption. This research aims to identify the potential of patikala leaf extract as an α-glucosidase enzyme inhibitor. Using an experimental method with patikala leaf samples collected from Mariri Hamlet, Banyuurip Village, Bone-bone District, North Luwu Regency. Extraction was performed using the maceration method with variations in solvents: 70% ethanol, ethyl acetate, and n-hexane. The α-glucosidase enzyme inhibition activity was measured using a microplate reader and analyzed to determine the IC50 value. From the percentage inhibition data, the IC50 values obtained for 30% ethanol extract of patikala leaves were 351.95 µg/mL, for 70% ethanol extract of patikala leaves were 444.83 µg/mL, for 96% ethanol extract of patikala leaves were 362.27 µg/mL, for ethyl acetate extract of patikala leaves were 163.59 µg/mL, and for n-hexane extract of patikala leaves were 294.90 µg/mL, indicating that patikala leaf extracts with variations in solvent or extraction method exhibited very weak α-glucosidase enzyme inhibition activity. In conclusion, based on the research results obtained, the extract with variations in the solvent or extractant. Patikala leaf extract has an inhibitory activity against the α-glucosidase enzyme, which is categorized as very weak because it has an IC50 value > 100 µg/mL. In comparison, the control (acarbose) is in the weak category with an IC50 value of 50-100 µg/mL.
Co-Authors Astuti Amin Aulia Debby Pelu Fadel Ahmad Haris Indri Indri Nurzadrina Wahyuddin Rahmah Mustarin Tuti Handayani Yuniar Dwi Yanti Yuri Pratiwi Utami Zahira Amody