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All Journal Jurnal NERS Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education JPSCR : Journal of Pharmaceutical Science and Clinical Research JURNAL AGRICA Biota Majalah Patologi Indonesia Medical and Health Journal
Hidayat Sulistyo
Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health

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The Expression of mRNA LMP1 Epstein-Barr Virus from FFPE Tumour Biopsy: a Potential Biomarker of Nasopharyngeal Carcinoma Diagnosis Wahyono, Daniel Joko; Gumilas, Nur Signa Aini; Sulistyo, Hidayat
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9028

Abstract

Nasopharyngeal carcinoma (NPC) is a multifactorial disease that is endemic geographically in the world. Indonesian population has a highly incidence rate that is 6.2/100,000 people year. The pathogenesis of NPC is more directly reflected by carcinoma-specific viral transcriptional activity at the site of primary tumour. Epstein-Barr virus (EBV) infection in NPC is reflected by the expression of EBV latent and lytic gene. In fact, mRNA Latent Membrane Protein 1 (LMP1) EBV expression was an important latent infection biomarker. The aim of this study was to determine a potential use of relative expression of mRNA LMP1 EBV from formalin-fixed paraffin embedded (FFPE) tumour biopsy in NPC as a tumour biomarker. This reseach design was a cross sectional study. The samples were the archived specimens of FFPE tumour biopsy from NPC WHO-3 patient which were collected from untreated patients from 2014 in the Department of Pathology Anatomy, Prof. dr. Margono Soekarjo Hospital, Purwokerto. The expression of mRNA LMP1 EBV expression was determined by RT-PCR technique. The positivity of mRNA LMP1 EBV expression was 51.9%, indicating a moderate positivity. The result proved that the expression of mRNA LMP1 EBV from FFPE NPC WHO-3 tumour biopsy was a potential biomarker of NPC diagnosis. The molecular methods would improved the management of NPC, particularly in the histopathological diagnosis of NPC.
Penghambatan aktivitas proliferasi sel dan perubahan histopatologik epitel mukosa nasofaring mencit C3H dengan pemberian ekstrak benalu teh Hidayat Sulistyo; Awal Prasetyo; Tjahjono
Majalah Patologi Indonesia Vol 19 No 1 (2010): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.128 KB)

Abstract

ABSTRAKLatar belakangEfek scurrulla atropurpurea (SA) sebagai anti kanker telah banyak dibuktikan hingga tingkat molekuler, namunsebagaian besar dilakukan secara in vitro. Penelitian ini bertujuan untuk membuktikan inhibisi aktivitas proliferasisel dan perubahan histopatologik epitel mukosa nasofaring mencit C3H dengan pemberian ekstrak SA.MetodePenelitian eksperimental menggunakan 15 mencit C3H, dibagi menjadi tiga kelompok: kelompok kontrol (K)diinduksi dengan uap larutan formalin 10%, diberi diet standar AIN-93M mengandung 54 mg/kg BB formalinselama 9 minggu; kelompok P1 diinduksi uap formalin dan diet standar berformalin selama 6 minggu, lalu diberi1,5 g/kgBB ekstrak SA selama 3 minggu; kelompok P2 diberi ekstrak SA selama 3 minggu, kemudian diinduksiuap formalin dan diet standar mengandung formalin selama 6 minggu. Di akhir penelitian dilakukan pemeriksaanhistopatologik dan hitung AgNOR.HasilSkor perubahan histopatologik epitel nasofaring kelompok K (3,72±0,46), P1 (2,07±0,62), dan P2 (1,69±0,48).Rerata hitung AgNOR kelompok K (3±0,15), P1 (1,72±0,07), dan P2 (1,76±0,08). Uji Mann-Whitney terhadapvariabel perubahan histopatologik antara P1 vs P2, K vs P2, dan P2 vs P3 bermakna. Uji Mann-Whitneyterhadap variabel hitung AgNOR antara K vs P1 dan K vs P2 bermakna, namun antara P1 vs P2 tidakbermakna.KesimpulanEkstrak SA sebelum dan sesudah induksi formalin menimbulkan pengaruh berbeda bermakna pada perbaikanskor histopatologik dan aktivitas proliferasi sel epitel mukosa nasofaring mencit C3H
Hepatoprotective Potential of Propolis toward Hepar Injury Rats (Rattus norvegicus) Induced by Carbon Tetrachloride Diah Krisnansari; Hidayat Sulistyo; Wahyu Dwi Kusdaryantoi
Jurnal Ners Vol. 9 No. 2 (2014): Oktober 2014
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3117.9 KB) | DOI: 10.20473/jn.v9i2.2575

Abstract

Introduction: The prevalence of chronic liver disease continues to increase. One potentially hepatotoxic substances that cause chronic liver disease is carbon tetrachloride. The process of liver damage can be prevented by the antioxidant role of propolis. The aims of this research was to study the hepatoprotective potential of propolis toward hepar injury rats induced by carbon tetrachlorida.Method: This was an experimental study with pre-post test. Twenty five male Wistar rats aged 12–16 week old, weighing 125–200 gr were allocated into 5 groups. Group I: standard meal + aquadest-gavage; group II: standard meal + CCl4 1% 1 mL + aquadest-gavage, group III: standard meal + CCl4 1% 1 mL + 0,054 gr propolis-gavage, group IV: standard meal + CCl4 1% 1 mL + 0,108 gr propolis-gavage and group V: standard meal + CCl4 1% 1 mL + sylimarin 50 mg/kg-gavage. IL-6, SOD, NAS score+fibrotic were measured after treatment. Analysed of IL-6 and NAS score+fibrotic with Kruskal Wallis to Mann Whitney and analysed of SOD with One-Way ANOVA to LSD.Results: The study showed that there were significant differences in IL-6, SOD and NAS score + fibrotic between groups.Discussion: Provision of 0,054 gr and 0,108 gr have hepatoprotective potential toward hepar injury rats induced by carbon tetrachlorida. Further research need to identify antioxidants and hepatoprotective potential of propolis on human with liver disease.
Ekspresi mRNA BRLF1 Virus Epstein-Barr dari Biopsi Jaringan Tumor Formalin-Fixed Paraffin Embedeed sebagai Petanda Biologi Molekul Diagnosis Karsinoma Nasofaring Arin Herkilini; Pancrasia Maria Hendrati; Nur Signa Aini Gumilas; Hidayat Sulistyo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.3.508

Abstract

Nasopharyngeal carcinoma (NPC) is a malignant tumor that grows in the nasopharynx with a predilection in the fossa Rosenmuller. Epithelial malignancies are often found in populations of China and Southeast Asia, including Indonesia. The NPC incidence in year 2008 as many as 84,400 cases and 51,600 of these cases resulted in death. A total of 120 new cases per year NPC found in hospitals Prof. dr. Margono Soekarjo (RSMS), Purwokerto. The NPC is difficult to be diagnose caused its primary tumor lies closed to the skull base as well as various structures of vital organs. Therefore, methods that can detect early NPC required for inspection.The etiology of NPC is multifactorial consisting of genetic factors, factors of infection Epstein-Barr Virus (EBV) and environmental factors.EBV has two phases in the cycle of infection that is the phase of lytic and latent phase.  BRLF1 has an important function as  mediator transition from latent e NPC. The research aimed  to analysis mRNA BRLF1 expression as a biomarker of  NPC diagnosis by RT-PCR and to determine the positivity of RT-PCR method to detect the expression of mRNA BRLF1. The research design was cross sectional study. Samples were FFPE tumor biopsy of NPC WHO III  and the total samples were 22 individu from Department of Pathology Anatomy,  Prof. Dr. Margono SoekarjoHospital, Purwokerto with informed consent. The positivity of mRNA BRLF1 from FFPE tumor biopsy of NPC WHO III  was in 63.6%indicating a high expression.
Ekspresi Relatif mRNA BRLF1 Epstein-Barr Virus dari Biopsi Jaringan Tumor dalam Blok Parafin sebagai Petanda Biologi Patogenesis Karsinoma Nasofaring Andreanne Poppy Estania; Hidayat Sulistyo; Daniel Joko Wahyono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.2.409

Abstract

Karsinoma nasofaring (KNF) merupakan tumor ganas pada sel epitel nasofaring dan merupakan penyakit multifaktor yang bersifat endemik. Tipe KNF tidak berdiferensiasi (KNF WHO-3) telah terbukti konsisten dengan infeksi EBV. Gen litik BRLF1 berperan sebagai mediator transisi dari fase laten menjadi litik. Pengukuran aktivitas mRNA EBV di lokasi tumor primer perlu dilakukan karena lebih mencerminkan patogenesis KNF yang  sesungguhnya dari pada diagnosis serologi dan pengukuran DNA EBV di sirkulasi darah. Penelitian ini bertujuan untuk mengetahui nilai ekspresi relatif mRNA BRLF1 Epstein-Barr Virus pada beberapa tingkat stadium tumor penderita KNF sebagai  petanda biologi potensial dalam patogenesis KNF. Metode penelitian yang digunakan adalah metode deskriptif yang berbentuk studi seran lintang. Sampel penelitian ini adalah biopsi jaringan tumor dalam blok parafin penderita KNF sebanyak 24 sampel. Sampel tersebut telah didiagnosis pasti sebagai KNF WHO-3 dari hasil pemeriksaan Patologi Anatomi pada Poli Patologi Anatomi, RSUD Prof. Dr. Margono Soekarjo, Purwokerto serta memenuhi kriteria inklusi sampel. Analisis statistik dilakukan untuk membandingkan ekspresi relatif mRNA BRLF1 stadium yang berbeda. Nilai ekspresi relatif mRNA BRLF1 EBV pada 24 sampel KNF berkisar 99,04415959-1097,496026. Hasil analisis statistik menunjukkan nilai ekspresi relatif mRNA BRLF1 EBV pada stadium awal (n = 5; 544,72420 + 142,614733) lebih tinggi 1,8 kali dibandingkan dengan stadium lanjut (n = 17; 395,68612 + 293,172201), namun peningkatan  ekspresi relatif mRNA BRLF1 EBV tidak bermakna secara statistik karena didapatkan nilai p=0,130 (p>0,05). Oleh karena itu, ekspresi relatif mRNA BRLF1 EBV dari biopsi jaringan tumor dalam blok parafin tidak berpotensi sebagai petanda biologi molekul patogenesis KNF, khususnya progresivitas tumor pada stadium lanjut KNF.
Ekspresi mRNA LMP2A Epstein-Barr Virus dari Biopsi Jaringan dalam Blok Parafin Berpotensi sebagai Biomarka dalam Diagnosis Karsinoma Nasofaring Roni Afriansya; Hendro Pramono; Hidayat Sulistyo; Daniel Joko Wahyono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 3 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.3.323

Abstract

Karsinoma nasofaring (KNF) merupakan tumor epitel yang terletak di nasofaring dan merupakan penyakit genetik multifaktor yang endemik. Penyebab utama KNF adalah infeksi oleh Virus Epstein-Barr (EBV). Keberadaan EBV pada penderita KNF dapat diketahui dengan ditemukannya DNA EBV dalam spesimen biopsi jaringan penderita KNF. Genom EBV dan ekspresi sebagian dari produk gen laten virus secara konsisten terdeteksi hampir di setiap sel dalam kanker ini, salah satunya adalah gen Latent Membrant Protein (LMP). Aktivitas mRNA EBV lebih mencerminkan patogenesis KNF yang  sesungguhnya dari pada diagnosis serologi dan pengukuran DNA EBV di sirkulasi. Penelitian dilakukan di Laboratorium PA RSUD Margono Seokarjo, Purwokerto/Lab PA Fakultas Kedokteran dan laboratorium genetika/molekuler Universitas Jenderal Soedirman Purwokerto. Analisis Ekspresi mRNA LMP2A EBV dengan teknik one step RT-PCR dan produk RT-PCR (amplikon cDNA) divisualisasi dengan elektroforesis gel agarosa 1%. Hasil ekspresi mRNA LMP2A EBV adalah 27,3% (6 dari 22 sampel). Kesimpulan, metode one step RT-PCR dapat digunakan untuk menganalisis ekspresi mRNA LMP2A EBV dari sampel biopsi jaringan KNF dalam blok paraffin dan hasil positivitas ekspresi mRNA LMP2A EBV sedang, sehingga berpotensi digunakan sebagai petanda biologi molekul diagnosis KNF.
Pengaruh Pemberian Kombinasi Ekstrak Etanol Brotowali, Sambiloto, Meniran dan Kayu Manis Terhadap Histopatologi Glomerulus Tikus Model Hiperglikemia Dhadhang Wahyu Kurniawan; Novianti Dian Lestari; Hidayat Sulistyo; Cacu Cacu
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 7, No 3 (2022)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v7i3.51501

Abstract

Brotowali, sambiloto, meniran, dan kayu manis merupakan tanaman unggulan Indonesia yang diketahui memiliki kandungan metabolit sekunder yang efektif dalam menurunkan kadar glukosa darah. Keempat tanaman tersebut telah banyak diteliti dan digunakan untuk mengatasi kondisi hiperglikemia namun keamanan kombinasi keempat tanaman tersebut belum diketahui. Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian kombinasi ekstrak etanol keempat tanaman tersebut terhadap perubahan histopatologi glomerulus tikus wistar jantan yang mengalami hiperglikemia akut. Tikus Wistar jantan diinduksi hiperglikemia dengan glukosa 2 gram/kgBB kemudian diberi kombinasi ekstrak etanol brotowali, sambiloto, meniran, dan kayu manis dengan dosis terendah (100 gram/kgBB , 100 gram/kgBB , 200 gram/kgBB , dan 300 gram/kgBB ) dan tertinggi (500 gram/kgBB , 400 gram/kgBB , 400 gram/kgBB , dan 600 gram/kgBB ) pada fase akut selama 24 jam. Perubahan histopatologi yang diamati berupa degenerasi albumin, degenerasi vakuolar, dan perubahan ruang Bowman. Data hasil pengamatan dianalisis menggunakan uji Kruskal-Wallis dan Mann-Whitney dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa pemberian kombinasi ekstrak memberikan pengaruh terhadap perubahan histopatologi glomerulus tikus hiperglikemia dengan p value 0,001 untuk degenerasi albumin dan degenerasi vakuolar; 0,048 untuk pelebaran ruang Bowman; dan 0,020 untuk penyempitan ruang Bowman. Pemberian kombinasi ekstrak dosis rendah memberikan pengaruh terhadap kerusakan glomerulus yang lebih ringan dibandingkan pemberian kombinasi ekstrak dosis tinggi.
RISET PASAR PUPUK KASGOT Rifki Andi Novia; Purwanto; Loekas Susanto; Budi Prakoso; Ismangil; Muhammad Rif'an; Ratri Noorhidayah; Ruly Eko Kusuma Kurniawan; Hidayat Sulistyo; Aditya Hani
JURNAL AGRICA Vol. 16 No. 2 (2023): JURNAL AGRICA
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agrica.v16i2.8562

Abstract

Pupuk Kasgot merupakan pupuk organik berasal dari budidaya maggot Black Soldier Fly (BSF) yang perlu dikembangkan dan dipasarkan kepada petani. Tahap awal pengembangan pasar pupuk Kasgot melalui riset pasar sebagaimana dalam tujuan penelitian, yakni menentukan target pasar, mengetahui sikap petani dan informasi pasar, serta potensi pasar pupuk Kasgot. Metode analisis deskriptif digunakan dengan pengumpulan data menggunakan pendekatan kualitatif melalui observasi dan wawancara. Data riset pasar dikumpulkan melalui kuesioner, dengan uji validitas dan uji realibilitas. Data dianalisis menggunakan lembar kerja sebagai alat untuk merepresentasikan apa yang ingin diketahui dan diteliti. Hasil analisis lembar kerja dapat disimpulkan bahwa target pasar pupuk Kasgot yakni petani usia produktif, luas lahan kurang dari 5.000 meter persegi, status lahan milik sendiri, jenis lahan merupakan lahan basah dengan komoditas yang ditanam yakni padi dan hortikultura. Dalam aspek sikap, pemahaman petani terhadap pupuk Kasgot tergolong rendah, namun setelah adanya sosialisasi, aspek perasaan dan kecenderungan bertindak petani untuk mencoba sangat besar. Pupuk Kasgot juga memiliki potensi pasar untuk dapat bersaing dengan pupuk organik lainnya. Salah satu strategi pemasarannya berupa sosialisasi dan pengenalan kepada petani melalui pertemuan kelompok tani dan dipraktekan pada lahan percontohan (demplot).
KADAR ALPHA FETOPROTEIN (AFP) DENGAN PROFIL HbsAg PADA PASIEN KARSINOMA HEPATOSELULER DI RSUD PROF. DR. MARGONO SOEKARJO: SEBUAH STUDI CROSS SECTIONAL Yudiananda, Domas Shifa; Sulistyo, Hidayat; Wijayana, Kamal Agung
Medical and Health Journal Vol 4 No 1 (2024): August
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mhj.2024.4.1.12894

Abstract

Background: Hepatocellular carcinoma is 10-20% of all liver diseases in Indonesia. Detect the emergence of hepatocellular carcinoma in the early phase are needed, especially in patients with high risk, such as chronic hepatitis and cirrhosis of the liver by performing regular investigations (USG, CT Scan) and laboratory examinations (AFP, PIVKA II). Aim: To determine the correlation between AFP levels and HbsAg profile in Hepatocellular Carcinoma patients at Regional Public Hospital of Prof. Dr. Margono Soekarjo. Methods: The research method was analytic observational with cross sectional approach. This research uses samples from January-December in 2020. The sampling method of this research is Total sampling. Retrospective data retrieval of secondary data in the form of medical records of the research sample. The research subjects were Hepatocellular Carcinoma patients at Prof. Dr. Margono Soekarjo, totaling 30 people. Result: The mean of people with hepatocarcinoma was 22 men with a percentage of 73.33%, while 8 people were female with a percentage of 26.67%. The mean age of the subjects of this study was 49.5 years. Subjects who had reactive results on HBsAg examination were 21 people with a percentage of 70%. The mean AFP level of the subjects was 17379.37 with a standard deviation of 25968.78. Results of the Fisher test for the correlation between AFP and HbsAg profile in hepatocellular carcinoma patients had a significance value of 0.032 (p <0.05). Conclution: that there is a correlation between AFP levels and HBsAg profile in Hepatocellular Carcinoma patients at Regional Public Hospital Prof. Dr. Margono Soekarjo.
Relative Expression of mRNA BARF1 Epstein-Barr Virus from Tumor Tissue Biopsy in Formalin-Fixed Paraffin-Embedded in Nasopharyngeal Carcinoma Wahyono, Daniel Joko; Fuadi, Muflih; Susanto, Agus Hery; Sulistyo, Hidayat
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.413

Abstract

Nasopharyngeal carcinoma (NPC) is a malignant tumor originating from nasopharyngeal epithelial cells. NPC is endemic in some regions, especially in Southeast Asia countries. NPC is also a multifactorial disease involving environmental factors, genetic factors, and infection from Epstein-Barr virus (EBV). According to WHO classification, Undifferentiated NPC is histopathologically associated with EBV infection and categorized into WHO subtype 3. EBV has two phases in its infection cycle: the lytic and latent phases. The BARF1 gene is a mediator of the transition from the latent phase to the lytic phase. Previous studies suggest measurement of EBV mRNA activity at the primary tumor site in the nasopharyngeal reflects the pathogenesis of NPC rather than measuring circulating EBV DNA or serological diagnosis. This study aimed to determine the relative expression potential of BARF1 mRNA at different tumor stages in NPC patients as a predictor of NPC pathogenesis. This research design was a descriptive research method in the form of a cross-sectional study. The samples used were 22 patients diagnosed as NPC WHO class III at the Anatomical Pathology Section of Prof. Dr. Margono Soekarjo, Purwokerto, who met the inclusion criteria. The relative expression of BARF1 mRNA was carried out using the one-step real-time RT-PCR technique and then calculated using a formula of 2-ΔCt. The T-test was used to compare the relative expression of early and late-stage BARF1 mRNA. The relative expression of BARF1 mRNA in the late-stage advanced stage (n = 6; 0.708292-0.840177; med = 0.7164655) was increased compared to the early stage (n = 2; 0.708841-0.712423; med = 0. 710632).
Co-Authors Aditya Hani AGUS HERY SUSANTO Andreanne Poppy Estania Arin Herkilini Awal Prasetyo Budi Prakoso Cacu Cacu Daniel Joko Wahyono Dhadhang Wahyu Kurniawan Diah Krisnansari Fuadi, Muflih Gumilas, Nur Signa Aini Hendro Pramono Ismangil Kamal Agung Wijayana, Kamal Agung Loekas Susanto Muhammad Rif'an Novianti Dian Lestari Nur Signa Aini Gumilas Pancrasia Maria Hendrati Purwanto Ratri Noorhidayah Rifki Andi Novia Roni Afriansya Ruly Eko Kusuma Kurniawan Tjahjono Wahyu Dwi Kusdaryantoi Yudiananda, Domas Shifa