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All Journal Meditory : The Journal of Medical Laboratory Jurnal Analis Medika Biosains (JAMBS) Lambda: Jurnal Ilmiah Pendidikan MIPA dan Aplikasinya
Ersandhi Resnhaleksmana
Poltekkes Kemenkes Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Mathematics Physics Public Health Other

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CORRELATION OF NEUTROPHIL LYMPHOCYTE RATIO WITH HbA1C IN DIABETIC ULCERS Ni Nengah Dwi Putri Meili Wahyuni; Ersandhi Resnhaleksmana; Fihiruddin Fihiruddin
Meditory : The Journal of Medical Laboratory Vol. 14 No. 1 (2026): Meditory, Volume 14 No. 1 Tahun 2026
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v14i1.4104

Abstract

Background: Patients diagnosed with diabetes are susceptible to complications, including diabetic ulcers. HbA1C is one of the key parameters used to evaluate glycemic control At the same time, the neutrophil-to-lymphocyte ratio (NLR) is a test used to detect inflammatory processes in patients with diabetes mellitus who develop diabetic ulcers. Objective: This research analyzes the relationship between the neutrophil-to-lymphocyte ratio (NLR) and HbA1C levels in patients with diabetes mellitus and diabetic foot ulcers. Methods: The research has a cross-sectional design. Data were collected retrospectively using non-probability sampling through a purposive sampling method from January 2024 to January 2025. Data were obtained from 83 individuals diagnosed with diabetes mellitus, including those with diabetic ulcer. Results: The highest NLR value was 26.47 and the lowest was 1.14. The highest HbA1C value was 51.0, while the lowest was 5.40. Spearman's statistical test indicated no significant correlation between NLR and HbA1C in patients with diabetes mellitus and diabetic foot ulcers (p = 0.534 > 0.05). Conclusions: There is no correlation between the neutrophil-to-lymphocyte ratio (NLR) and HbA1C levels in patients with diabetes mellitus and diabetic foot ulcers.
Differences In Hemoglobin Levels In Infected Patients Salmonella Typhi With Salmonella Paratyphi Based On Antibody Titer Laelin Hijriyani; Fihiruddin Fihiruddin; Iswari Pauzi; Ersandhi Resnhaleksmana
Jurnal Analis Medika Biosains (JAMBS) Vol. 13 No. 01 (2026): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

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Salmonella typhi and Salmonella paratyphi cause the disease known as typhus abdominalis. The pathogenicity that distinguishes Salmonella typhi and Salmonella paratyphi comes from the virulent part of the bacterial capsule. Virulence factors are found on the surface of Salmonella typhi that are not found in Salmonella paratyphi. The toxic effect of Salmonella that suppresses the bone marrow and the presence of bleeding in the intestine can cause anemia, so a supporting examination is needed, one of which is the examination of Hemoglobin levels. The aimt of this study is knowing the difference of hemoglobin levels in patients infected with Salmonella typhi and Salmonella paratyphi based on antibody titer against H antigens: This study used Analytical Observational method with cross-sectional approach. Data obtained from typhoid fever patients from the examination of hemoglobin levels at Patut Patuh Patju Hospital as many as 96 people using secondary data.  "The mean hemoglobin level for Salmonella typhi, Salmonella paratyphi A, and Salmonella paratyphi B was 9.7 g/dL, 12.0 g/dL, and 12.6 g/dL, respectively. At antibody titers of 1/160 and 1/320, the mean hemoglobin levels for S. typhi were 10.5 g/dL and 9.2 g/dL; for S. paratyphi A, 12.0 g/dL and 11.8 g/dL; and for S. paratyphi B, 12.8 g/dL and 11.6 g/dL. According to the ANOVA test, there is a significant difference in the average hemoglobin levels among patients infected with S. typhi compared to those with S. paratyphi based on these antibody titers (< 0,001)
Policy Implementation Studies of the Tuberculosis Control Program Towards the Elimination of Tuberculosis in East Lombok Regency, West Nusa Tenggara Province Ersandhi Resnhaleksmana; Maruni Wiwin Diarti; Ni Putu Karunia Ekayani; Yudha Anggit Jiwantoro; Iswari Pauzi
Lambda: Jurnal Ilmiah Pendidikan MIPA dan Aplikasinya Vol. 6 No. 2 (2026): Lambda
Publisher : Lembaga Bale Literasi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58218/lambda.v6i2.4265

Abstract

The NTB Provincial Health Office recorded that East Lombok Regency, with 35 Health Centers, had 7,331 suspected tuberculosis (TB) patients who received standard services, comprising 432 male cases (54.3%), 363 female cases (45.7%), totalling 795 cases, and 67 TB cases among children aged 0–14 years. The role of academics and stakeholders in implementing the 2020–2024 national TB control strategy is critically important. This study aimed to examine the implementation of the TB program control policy in East Lombok Regency. A qualitative approach was employed with 79 informants using in-depth interviews, group interviews, and FGDs. Informants included TB program managers at the district level, Health Office officials, health center staff, and TB patients. Six health centers were selected purposively: Terara and Masbagik Baru (high achievement), Lenek and Aikmel (medium achievement), and Selong and Lendang Nangka (low achievement). The study was conducted from August to October 2023. Results revealed that TB program control policy implementation has been carried out well, supported by community participation, cross-sector coordination, availability of facilities and funds, and communicative communication. However, local government funding ceased after COVID-19, hampering training, active case finding, and community counseling activities. Policy recommendations include reinstating local government funding and adding TCM (Tes Cepat Molekuler) tools and dedicated TB waiting and sampling rooms
Comparison of DNA Extraction Results from Staphylococcus aureus Using the Boiling Method and the Combined BoilingCentrifugation-Bead Method in Terms of DNA Quality for PCR Testing Baiq Inges Mustika Suci; Fihiruddin; Ersandhi Resnhaleksmana
Jurnal Analis Medika Biosains (JAMBS) Vol. 13 No. 02 (2026): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

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Abstract

DNA extraction is a critical step in molecular testing based on the Polymerase Chain Reaction (PCR). In Gram-positive bacteria such as Staphylococcus aureus, the DNA extraction process requires an effective lysis method due to their thick peptidoglycan layer. The boiling method is simple and economical; however, further development of extraction methods is still needed to improve the quality of the resulting DNA. This study aimed to compare the results of DNA extraction using the boiling method and the combined boilingcentrifugation-bead method in terms of the quality of Staphylococcus aureus DNA for PCR testing. This was a laboratory-based experimental study with a comparative design using 30 samples of Staphylococcus aureus bacterial suspensions standardized to a McFarland turbidity of 1.5. Fifteen samples were extracted using the boiling method, and 15 samples were extracted using the combined boiling-centrifugation-bead method. DNA quality was measured using a NanoDrop, including DNA concentration, DNA purity as determined by the A260/A280 ratio, and the A260/A230 ratio. The DNA’s ability to serve as a template was also evaluated through PCR amplification of the nuc gene. Data analysis was performed using the Independent Samples t-test and the Mann–Whitney U test. The boiling method yielded a mean DNA concentration of 12.57 ng/µL, a mean A260/A280 ratio of 2.2433, and a median A260/A230 ratio of -2.13. The combined boiling-centrifugation-bead method yielded a mean DNA concentration of 50.19 ng/µL, a mean A260/A280 ratio of 1.4220, and a median A260/A230 ratio of 0.89. Statistical analysis revealed significant differences in DNA concentration, DNA purity as indicated by the A260/A280 ratio, and the A260/A230 ratio between the two extraction methods (p<0.001). PCR results showed the formation of a target DNA band from the nuc gene measuring approximately 423 bp using both DNA extraction methods. The combined boiling-centrifugation-bead method yielded a higher DNA concentration and a better A260/A230 ratio compared to the boiling method.
Co-Authors Baiq Inges Mustika Suci Fihiruddin, Fihiruddin Iswari Pauzi Laelin Hijriyani Maruni Wiwin Diarti Ni Nengah Dwi Putri Meili Wahyuni Ni Putu Karunia Ekayani Yudha Anggit Jiwantoro