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All Journal Jurnal Agroteknos Perbal: Jurnal Pertanian Berkelanjutan Wallacea Plant Protection Journal
ARIYANTI, EKA LESTARI
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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UJI POTENSI DAN MEKANISME ANTAGONIS BAKTERI ENDOFIT ASAL TUMBUHAN LIAR SEBAGAI AGENS HAYATI PENGENDALIAN Fusarium oxysporum SECARA IN-VITRO RAHIMI, ANDI SITTI; KHAERUNI, ANDI; BANDE, LA ODE SANTIAJI; ARIYANTI, EKA LESTARI; SATRAH, VIT NERU; ASNIAH, ASNIAH
Jurnal Agroteknos Vol 12, No 2 (2022)
Publisher : Jurnal Agroteknos

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Abstract

This research motivated by the Fusarium oxysporum which is a soil borne fungus that can infect cultivated plants. F. oxysporum is a facultative parasite that is part of its life as a saprophyte and becomes a parasite if there is a host plant. This study aims to determine the potential of endophytic bacteria from wild plants as biological agents control of F. oxysporum in-vitro. This research was conducted at the Laboratory of Plant Protection, Phytophatology Unit, Faculty of Agriculture, Halu Oleo University from September 2020 to March 2021. The results showed that endophytic bacteria isolated from wild plants such as as Jamaica cherry/kersen (Muntingia calabura), Suruhan (Paperomia pellucida), and Markisa Hutan/Rambusa (Passiflora foetida) have the potential as biological agents to control F. oxysporum in vitro, with the two best isolates, namely ASR-7(3) and BK-3(2) which were to inhibit the growth of F. oxysporum through the mechanism of antibiosis and competition, produced enzyme bioactive compounds (amylase and protease) which degrade of pathogenic fungi, and are able to produce volatile compounds.
PENGENDALIAN HAYATI PENYAKIT LAYU FUSARIUM PADA TANAMAN TOMAT (Solanum lycopersicum L.) MENGGUNAKAN BAKTERI ENDOFIT ASAL TANAMAN LIAR NERU SATRAH, VIT; ASDAR, ASDAR; KHAERUNI, ANDI; SANTIAJI BANDE, LA ODE; RAHMAN, ABDUL; ISNAINI ULFA, NUR; ARIYANTI, EKA LESTARI
Jurnal Agroteknos Vol 13, No 3 (2023)
Publisher : Jurnal Agroteknos

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This research aims to determine the effect of using endophytic bacteria from wild plants in the biological control of Fusarium Wilt disease on tomato plants and to find out which isolate method is best for controlling Fusarium Wilt disease on tomato plants. The research was carried out at the Plant Protection Laboratory, Phytopathology Unit, and Experimental Garden Greenhouse 1, Faculty of Agriculture, Halu Oleo University, Kendari, from September to November 2022. This research used a Randomized Block Design (RBD) with 5 treatments repeated 3 times for a total of 15 treatment units. The variables observed were divided into 2, namely vegetative plant growth (plant height, number of leaves, and number of productive branches) and the incidence of Fusarium Wilt disease on tomato plants. The data was analyzed using variance and if there was a real influence, it was continued with the Duncan Multiple Range Test (DMRT) at a confidence level of 95%. The results of the research showed that endophytic bacteria from wild plants were able to increase plant growth and control Fusarium Wilt disease in tomato plants and ASR-3(2) isolate was the best isolate in increasing plant growth and controlling Fusarium Wilt disease.
UJI BEBERAPA MINYAK ATSIRI SEBAGAI ATRAKTAN LALAT BUAH (Bactrocera sp.) PADA TANAMAN TOMAT) ARIYANTI, EKA LESTARI; SAYANG, YULIS; A, SYAMSURIATI
Jurnal Agroteknos Vol 12, No 3 (2022)
Publisher : Jurnal Agroteknos

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This research was conducted to determine the effectiveness of this type of essential oil as an attractant for fruit flies (Bactrocera sp.) on tomato plants. The research was carried out on farmer's land in Benteng Alla Utara Village, Baroko District, Enrekang Regency. The implementation of this research starts from April 2021 until it is finished. This experiment used a randomized block design (RBD) consisting of five treatments with four replications. The treatments included citronella essential oil, clove essential oil, basil essential oil, tomato extract and also control. In testing the effectiveness of several essential oils obtained by steam distillation distillation technique. Each trap dripped with 3 ml of essential oil. Observation of trapped fruit fly populations was carried out on plants 49 days, 56 days, 63 days, 70 days, 77 days, and 84 days after planting. The results showed that the essential oil of basil was the most effective as an attractant for fruit flies on tomato plants with an average population of 55.17%.
POTENSI EKSTRAK DAUN SIRIH (Piper betle liin) SEBAGAI BIOFUNGISIDA PENYAKIT BUSUK BUAH STROBERI (Colletotrichum fragariae brooks) SECARA IN-VITRO Ariyanti, Eka Lestari; Jahuddin, Rahmat; Yunus, Muhammad
Jurnal Agroteknos Vol 2, No 3 (2012)
Publisher : Jurnal Agroteknos

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One of important pathogens on strawberry is Colletotrichum fragariae causing fruit rot. The study aimed to investigate the potency of water betel leaf extract (Piper betle liin) as biofungicides for inhibiting growth of C. Fragariae under   in-vitro test.   The study was arranged in Completely Randomized Design that consisted of five treatments : 0%, 10%, 20%, 30%, and 40%  concentration of water betel leaf extract.  Betel leaf was extracted by grinded in mortal, added steril water and mixed with PDA medium.  The study showed that the 40% concentration of  water betel leaf axtraction was most effective to inhibit growth of C. Fragariae in-vitro on PDA medium. The inhibiting ability of coloni grown was 58,57%, while inhibiting of spore germination was 33,59%. Key word :  Colletotrichum fragariae, strawberry, water betel leaf extract.
Isolasi dan Uji Patogenesitas Cendawan Endofit Asal Tanaman Pisang Kepok (Musa acuminate balbisiana colla): Isolation and Inhibition Test of Endophytic Fungi from Kepok Banana (Musa acuminate balbisiana colla) Eka Lestari Ariyanti; Rahmat Jahuddin; Asti Irawanti Azis
Perbal: Jurnal Pertanian Berkelanjutan Vol. 13 No. 1 (2025): Perbal: Jurnal Pertanian Berkelanjutan
Publisher : Fakultas Pertanian Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/perbal.v13i1.5429

Abstract

Cendawan endofit dapat digunakan sebagai agens hayati dalam memperbaiki pertumbuhan dan ketahanan tanaman dalam menghadapi serangan penyakit. Penelitian ini bertujuan mengisolasi cendawan endofit asal tanaman pisang dan menguji potensinya sebagai pemacu pertumbuhan tanaman. Berdasarkan hasil isolasi cendawan endofit pada jaringan tanaman pisang kepok diperoleh sebanyak 6 isolat. Hasil identifikasi dan karakterisasi menunjukkan terdapat 4 genus cendawan. Genus cendawan tersebut yaitu Culvularia sp., Fusarium sp., Aspergillus sp., dan Trichoderma sp. Isolat cendawan tersebut diseleksi untuk melihat kemampuan dalam memacu pertumbuhan tanaman melalui uji patogenesitas. Hasil uji patogenesitas menunjukkan cendawan dari genus Trichoderma sp., dan Fusarium sp. memiliki kemampuan terbaik dalam memacu pertumbuhan tanaman. Hal ini mengindikasikan bahwa beberapa cendawan endofit non patogen asal tanaman pisang berpotensi sebagai pemacu pertumbuhan tanaman. Endophytic fungi can be used as biological agents in improving plant growth and resistance in the face of disease attacks. This study aims to isolate endophytic fungi from banana plants and test their potential as a booster for plant growth. From the results of the isolation of endophytic fungi in the banana pupace plant tissue, 6 isolates were obtained. The results of identification and characterization show that there are 4 genera of fungi. The genus of fungi is Culvularia sp., Fusarium sp., Aspergillus sp., and Trichoderma sp. The fungus isolates were selected to see the ability to spur plant growth through pathogenicity tests. Pathogenicity test results show that fungi from the genus Trichoderma sp., and Fusarium sp. have the best ability to spur plant growth. This indicates that some non-pathogenic endophytic fungi from banana plants have the potential to be a driver of plant growth.
Comparative antifungal efficacy of Zinnia elegans and Tithonia diversifolia extracts against Fusarium verticillioides: In-vitro study Asmila, Asmila; Azis, Asti Irawanti; Ariyanti, Eka Lestari; Kumalasari, Ade Sugiarti
Wallacea Plant Protection Journal Vol. 1 No. 2 (2025)
Publisher : Department of Plant Pest and Diseases, Faculty of Agriculture, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64128/wppj.v1i2.48421

Abstract

Fusarium verticillioides are broad-spectrum pathogens with extensive host ranges and represent a major constraint in agricultural production. Synthetic pesticides are commonly used by farmers to manage Fusarium-induced diseases; however, their intensive application poses serious risks to environmental sustainability and human health. Consequently, the development of eco-friendly disease management strategies is urgently required. Botanical pesticides derived from plant extracts represent a promising alternative. This study evaluated the antifungal effectiveness of Mexican sunflower (Tithonia diversifolia) and zinnia (Zinnia elegans) extracts at different concentrations against Fusarium wilt. The experiment was conducted from September to December 2024 using a completely randomized design (CRD) with six extract treatments (1800, 3600, and 5400 ppm for each plant species) and an untreated control. Antifungal activity was assessed based on the percentage of mycelial growth inhibition. The results demonstrated that both extract type and concentration significantly affected fungal inhibition (p ≤ 0.05). Extract of Z. elegans at 5400 ppm exhibited the highest inhibitory effect, suppressing Fusarium growth by 32.0%, which was significantly higher than all other treatments. In contrast, T. diversifolia extract showed moderate inhibition, with a maximum of 16.5% inhibition at 5400 ppm. The increasing inhibition with higher concentrations indicates a clear dose-dependent response. These findings highlight the scientific significance of plant-based extracts as natural antifungal agents and demonstrate the superior efficacy of Z. elegans extract at higher concentrations. Overall, this study underscores the strong potential of zinnia-based extracts as eco-friendly biofungicide candidates for sustainable management of Fusarium wilt diseases.
Co-Authors A, SYAMSURIATI Abdul Rahman ANDI KHAERUNI Asdar Asdar Asmila, Asmila Asniah Asniah Asti Irawanti Azis Asti Irawanti Azis ISNAINI ULFA, NUR Jahuddin, Rahmat Kumalasari, Ade Sugiarti La Ode Santiaji Bande Muhammad Yunus NERU SATRAH, VIT RAHIMI, ANDI SITTI Rahmat Jahuddin Satrah, Vit Neru sayang, yulis