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All Journal Indonesian Journal of Medical Chemistry and Bioinformatics
Kristanty, Diyah
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Chemistry Computer Science & IT

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Prothrombin Time (PT), Activated Partial Thromboplastin Time (APTT), Fibrinogen, and D-dimer in Coronavirus Disease 2019 Outcome Atmaja, Fredy Wirya; Adiyanti, Sri Suryo; Kristanty, Diyah; Dwira, Surya; Kusmardi, Kusmardi
Indonesian Journal of Medical Chemistry and Bioinformatics Vol. 3, No. 1
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COVID-19, caused by SARS-CoV-2 has been reported to be associated with coagulopathy and DIC. This study aimed to investigate the profiles and differences of PT, APTT, fibrinogen, and D-dimer in COVID- 19 outcome. This retrospective cohort was conducted at Central Laboratory Clinical Pathology Department of dr. Cipto Mangunkusumo Hospital from July – December 2020. Demographic, clinical, and laboratory data were extracted from EHR and compared between poor and good outcome. Ninety-seven subjects were confirmed positive COVID-19, 45 of whom (46.4%) were in poor outcome group, while 52 subjects (53.6%) were in good outcome group. Median of PT 11.0” (9.7-28.3), APTT 38.4” (23.9-121), fibrinogen 484.8 mg/dL (51.2-940.9), and D-dimer 1,800 µg/L (190-35,200). Longer PT, APTT, and higher D-dimer (p < 0.05), while lower fibrinogen (p > 0.05) was found in poor outcome group. There were significant differences of PT, APTT and D-dimer in COVID-19 outcome.
Phytochemical Profile and Cervical Anticancer Activity of an In Vitro n-Hexane Extract of Kunto Dewo Fruit (Kigelia pinnata) Peel and Flesh Isbandiputri, Swarnasari Nurandita; Dwira, Surya; kristanty, diyah
Indonesian Journal of Medical Chemistry and Bioinformatics Vol. 2, No. 1
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Cervical cancer is the fourth highest cancer occurring and causes death in women. Therefore, adequate management is needed to prevent its development. Currently, the treatment has a variety of adverse side effects, so it needs alternative treatments that are supportive and with minimal side effects. One way is to use herbal plants, such as the Kunto Dewo (Kigelia pinnata) plant which is often used as traditional medicine. This plant has antimicrobial and cytotoxic effects on cancer cells. Knowing the phytochemical profile and in-vitro anticancer activity of the n-hexane extract of peel and flesh of Kunto Dewo (Kigelia pinnata) fruit against cervical cancer HeLa cells. The peel and flesh of Kigelia pinnata fruit are macerated in n-hexane solvent then the resulting filtrate is evaporated to become an extract. The extract is used for phytochemical profile, carry out through phytochemical screening, thin layer chromatography, calculation of total phenol, and total flavonoids. The extract was also tested for cytotoxic activity against cervical cancer HeLa cells using MTT assay. The n-hexane extract of the peel and flesh of the kigelia pinnta fruit contains triterpenoids. In TLC analysis, there were found 4 components in the n-hexane extract of Kigelia pinnata fruit peel and 8 components in the n-hexane extract of Kigelia pinnata fruit flesh. The cytotoxic activity of the n-hexane extract of the peel and flesh of Kigelia pinnata fruit is included in the moderately active category. The n-hexane extract of the peel and flesh of the kigelia pinnata fruit has potential as an anti-cervical cancer.
In Silico Analysis of CD40 Mutations and Their Implications for Quinoline-benzoic acid derivatives Based Therapy in Graves' Disease Yunaini, Luluk; Kristanty, Diyah; Sari, Puji; Dwira, Surya; Suryandari, Dwi Anita; Bustami, Arleni
Indonesian Journal of Medical Chemistry and Bioinformatics Vol. 3, No. 2
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Graves' disease is an autoimmune disorder in which the CD40-CD154 interaction plays a critical role in T-cell activation. In this study, in silico methods were employed to analyze the binding interactions of quinoline-benzoic acid derivatives (NSB, FSB, and NQB) with the CD40 receptor and to investigate the implications of specific CD40 mutations for drug efficacy. In this reseach conducted by molecular simulation approach with molecular docking Results Mutation analysis of CD40 identified alterations in key residues, such as R203C, which may impact ligand-independent activation and downstream TRAF binding, crucial for signal transduction. These findings highlight the therapeutic potential of quinoline-benzoic acid derivatives for targeting CD40 in Graves' disease, particularly in the context of receptor mutations. The integration of molecular docking, mutation analysis, and pharmacokinetic profiling provides a comprehensive framework for designing effective CD40-targeted therapies.
Exploring Differentially Expressed Genes to Identify Biomarkers of Cervical Cancer: A Bioinformatics Approach Suryandari, Dwi Anita; Yunaini, Luluk; Kristanty, Diyah; Prawiningrum, Aisyah
Indonesian Journal of Medical Chemistry and Bioinformatics Vol. 4, No. 1
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This study explores the molecular landscape of cervical cancer through the identification and analysis of differentially expressed genes (DEGs) from the GSE63514 dataset. A high-confidence protein–protein interaction (PPI) network was constructed using the STRING database (v11.5) and visualized via Cytoscape, identifying 178 nodes and 1,052 edges. Using the CytoHubba plugin, the top 10 hub genes—TOP2A, MKI67, CDK1, BUB1, CCNB1, CCNA2, AURKA, CDC20, PLK1, and RFC4—were highlighted based on degree centrality. These genes are predominantly associated with cell cycle regulation, DNA replication, and mitotic division, and are potentially valuable as biomarkers or therapeutic targets for cervical cancer. Functional enrichment using DAVID and Enrichr tools revealed significant involvement of DEGs in ATP binding, spindle microtubule formation, and protein kinase activity, particularly within the chromosome centromeric region and nucleoplasm. KEGG pathway analysis identified key associations with the cell cycle, DNA replication, p53 signaling, and complement and coagulation cascades. Further heatmap analysis of treatment responders versus non-responders demonstrated distinct gene expression profiles, particularly of immune-related genes like C1QA, C3, and SERPING1, and proliferative markers such as TOP2A and MKI67. These findings underscore the dual role of immune and proliferative pathways in cervical cancer progression and suggest their utility in developing predictive biomarkers and personalized treatment strategies.
Phytochemisty and Antioxidant Activity of Ethanol, Ethyl Acetate, and n-Hexane Extracts of Suruhan Leaves (Peperomia pellucida) Arsianti, Ade; Gill, Steven; Kristanty, Diyah
Indonesian Journal of Medical Chemistry and Bioinformatics Vol. 4, No. 1
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Background: Free radicals are natural byproducts of cellular metabolism, in which, their excessive accumulation can lead to oxidative stress, which plays a key role in the development of various chronic diseases, including cancer, cardiovascular disorders, and neurodegenerative conditions. Antioxidants are compounds capable of scavenging free radicals and minimizing oxidative damage. Peperomia pellucida, commonly known as Suruhan leaves, is a tropical herb traditionally used in folk medicine that contain bioactive compounds correlated with a strong radical-scavenging activity, suggesting its potential as a natural antioxidant source. This study aims to conduct a phytochemical analysis and evaluate the antioxidant activity of ethanol, ethyl acetate and n-hexane extracts of Suruhan leaves (P. pellucida). Method: Suruhan leaf powder obtained from Situbondo, East Java, was macerated in three different solvents of ethanol, ethyl acetate and n-hexane to produce its corresponding ethanol extract, ethyl acetate extract, and n-hexane extract of P. pellucida. The phytochemical components in P. pellucida extracts were determined using phytochemistry test. Antioxidant activity of the extracts was assessed using the DPPH method. Results: P. pellucida extracts comprise alkaloids, flavonoids, tannins, steroids, and triterpenoids as its phytochemical constituents. The ethanol and ethyl acetate extracts of P. pellucida displayed extremely strong antioxidant properties on DPPH with IC50 value of 14.45 µg/mL and 22.12 µg/mL respectively, while the n-hexane extract exhibited moderate antioxidant activity with IC50 value of 102.71 µg/mL on DPPH. Conclusion: P. pellucida extracts containing several phytochemical constituents that showed moderate-to-strong antioxidant activity towards DPPH free radical.
Solvent-Dependent Cytotoxicity of Peperomia pellucida Leaf Extracts Against MCF-7 Breast Cancer Cells Arsianti, Ade; Pratama, Rafael Erlangga Bagas; Gill, Steven; Kristanty, Diyah
Indonesian Journal of Medical Chemistry and Bioinformatics
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Background: Breast cancer remains a major global health burden. Particularly in Indonesia, limitations of conventional chemotherapy, such as severe side effect, toxicity, and high cost, indicating the necessitate the development of alternative therapeutic agents. Peperomia pellucida, a medicinal plant rich in bioactive phytochemicals, has shown promising anticancer potential; however, its cytotoxic activity against MCF-7 breast cancer cells and the influence of solvent polarity remains underexplored. This study aimed to evaluate the cytotoxic effects of P. pellucida leaf extracts which are prepared using different polarity of solvents (ethanol, ethyl acetate, and n-hexane). Method: Dried leaf powder of P. pellucida was extracted via maceration, and the resulting extracts were tested against MCF-7 cells using the MTT assay. Cells were exposed to concentrations ranging from 3.125 µg/mL to 400 µg/mL, and cytotoxicity was expressed as IC₅₀ values derived from linear regression analysis. Results: The ethanol extract exhibited the the strongest cytotoxic activity (IC₅₀ = 10.68 µg/mL), followed by the ethyl acetate extract (IC50=17.81µg/mL), while the n-hexane extract showed weak activity (IC50 = 62.73 µg/mL). The superior activity of the ethanol extract is attributed to its broader phytochemical profile, including flavonoids, alkaloids, tannins, and triterpenoids, which may act synergistically to induce apoptosis and inhibit proliferation. Conclusion: P. pellucida demonstrates solvent-dependent cytotoxic activity, with ethanol extract showing the highest potential as a candidate for breast cancer therapy. Further studies are required to elucidate its molecular mechanisms.
Co-Authors Arleni Bustami Arsianti, Ade Atmaja, Fredy Wirya Dwi Anita Suryandari Gill, Steven Isbandiputri, Swarnasari Nurandita Kusmardi , Luluk Yunaini Pratama, Rafael Erlangga Bagas Prawiningrum, Aisyah Puji Sari Sri Suryo Adiyanti, Sri Suryo Surya Dwira