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All Journal Jurnal Riset Kesehatan Poltekkes Depkes Bandung Tropical Health and Medical Research Journal
Kawaitouw, Leberina
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Religion Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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The Influence of Knowledge, Attitudes, and Skills of Malaria Microscopists on the Accuracy of Laboratory Diagnosis Results Sahli, Indra Taufik; Kosasih, Kosasih; Wirawan, Chevie; Asrianto, Asrianto; Kawaitouw, Leberina
Tropical Health and Medical Research Vol. 6 No. 2 (2024): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v6i2.118

Abstract

Malaria remains a significant public health issue in Indonesia, characterized by high rates of illness and death. Proper laboratory diagnosis is one of the keys to malaria prevention efforts. Malaria microscopists' proficiency, knowledge, attitudes, and skills require regular assessment to maintain diagnostic quality. This study examines the correlation between malaria microscopists' knowledge, attitudes, and competencies and the accuracy of laboratory diagnostic outcomes at the Jayapura City Health Center. This study used quantitative methods with descriptive and verification approaches. Data were collected through questionnaires and laboratory diagnosis results. The study sample was 13 malaria microscopists at the Jayapura City Health Center. The data were analyzed with SPSS software. The results showed that most respondents had a good level of knowledge (76.9%). Almost half of the respondents had a good attitude (46.2%). Most respondents had a sufficient level of skill (61.5%). Interestingly, although most respondents (76.9%) could diagnose malaria very accurately, this study did not find an effect of malaria microscopists' knowledge, attitude, and skills on the accuracy of diagnosis. The knowledge level of malaria microscopists at the Jayapura City Health Center is good, but their attitudes and skills still need to be improved. It is recommended that microscope Officers routinely attend training to improve their knowledge, attitudes and skills.
PENGARUH VARIASI VOLUME SAMPEL DARAH DAN DIAMETER SEDIAAN DARAH TEBAL PADA PEMERIKSAAN Plasmodium falciparum Kawaitouw, Leberina; Mulia, Yuliansyah Sundara; Sulaeman, Sulaeman; Rahmat, Mamat
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 16 No 2 (2024): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v16i2.2540

Abstract

Pemeriksaan Plasmodium secara mikroskopik merupakan gold standart dalam diagnosis infeksi. Sampel darah yang akan diperiksa dibuat sediaan darah tebal dan tipis. Sediaan darah tebal menggunakan sampel darah lebih banyak sehingga pada kasus malaria ringan, parasit mudah ditemukan. Tujuan penelitian untuk mengetahui perbandingan antara volume sampel darah 8µl, 10µl dan 12µl pada diameter 1cm dan diameter 1,5cm terhadap jumlah parasit P.falciparum. Penelitian ini menggunakan metode quasi eksperimen. Bahan pemeriksaan adalah darah EDTA 3mL yang diperoleh dari pasien terkonfirmasi positif P.falciparum, dilakukan pembuatan sediaan darah tebal dengan volume darah 8µl, 10µl dan 12µl pada diameter 1cm dan 1,5cm yang di multipikasi sebanyak 30 sediaan. Selanjutnya sediaan diwarnai dengan Giemsa 3% dan diperiksa secara mikroskopik menggunakan metode perhitungan kuantitatif. Data dianalisis menggunakan uji statistik Anova dengan derajat kepercayaan 95% didapatkan nilai signifikan pada diameter 1cm nilai 0.106>0.005 maka hipotesa Ha diterima. Pada sediaan darah diameter 1,5cm nilai 0.000<0.005, maka hipotesa Ho diterima. Kesimpulan adalah tidak terdapat perbedaan hasil jumlah P.falciparum pada volume sampel darah 8µl, 10µl dan 12µl pada diameter 1cm dan terdapat perbedaan jumlah P.falciparum pada volume 8µl, 10µl dan 12µl pada diameter 1,5cm.
Co-Authors Asrianto, Asrianto KOSASIH KOSASIH, KOSASIH Mamat Rahmat Mulia, Yuliansyah Sundara Sahli, Indra Taufik Sulaeman Sulaeman Wirawan, Chevie