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Propolis from Mount Lawu: Dose-Dependent Antioxidant and Anti-Inflammatory Effects in a Rat Model of Wound Healing Proginova Dian Yudatama; Amru Sungkar; Ida Bagus Budhi Surya Adnyana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 3 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i3.1232

Background: Chronic wounds present a significant healthcare challenge, necessitating the exploration of effective adjuvant therapies. Propolis, a natural product derived from beehives, has demonstrated antioxidant and anti-inflammatory properties. This study investigated the dose-dependent effects of Mount Lawu propolis extract on oxidative stress and inflammation in a rat model of wound healing. Methods: A completely randomized experimental design was employed using male rats (Rattus norvegicus). Granulation tissue wounds were induced, and the rats were treated with varying doses of ethanol extract of Mount Lawu propolis. Immunohistochemical analysis was performed to assess the expression of Malondialdehyde (MDA) and Interleukin-6 (IL-6) in the wound tissue. Results: The results demonstrated a significant dose-dependent decrease in MDA and IL-6 expression in the propolis-treated groups compared to the control group. The high-dose propolis group exhibited the most substantial reduction in both MDA and IL-6 levels. Conclusion: Mount Lawu propolis extract exhibits potent antioxidant and anti-inflammatory effects in a dose-dependent manner, suggesting its potential as an adjuvant therapy for chronic wound management.

Successful Surgical Management of Partial Obstructive Ileus Secondary to Intra-abdominal Tumor: A Case Report Bambang Irwansyah; Ida Bagus Budhi Surya Adnyana
Open Access Indonesian Journal of Medical Reviews Vol. 5 No. 1 (2025): Open Access Indonesian Journal of Medical Reviews
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/oaijmr.v5i1.702

Partial obstructive ileus, characterized by incomplete intestinal blockage, can arise from various etiologies, including intra-abdominal tumors. These tumors, often compressing or obstructing the bowel lumen, necessitate prompt diagnosis and intervention to prevent severe complications. This case report presents a 42-year-old woman with partial obstructive ileus secondary to an intra-abdominal tumor, highlighting the diagnostic process, surgical management, and clinical outcomes. A 42-year-old female presented to the emergency department with abdominal distension, pain, and a two-day history of constipation. Physical examination revealed abdominal tenderness, and radiographic imaging confirmed partial obstructive ileus with pneumoperitoneum. Initial management focused on hemodynamic stabilization, fluid resuscitation, and nasogastric decompression. Subsequently, an exploratory laparotomy was performed, revealing an intra-abdominal tumor causing intestinal obstruction. Segmental tumor resection was successfully conducted, alleviating the obstruction. In conclusion, this case underscores the importance of considering intra-abdominal tumors in the differential diagnosis of partial obstructive ileus. Timely surgical intervention, including tumor resection, can effectively address the obstruction and improve patient outcomes. Further research is warranted to explore the long-term outcomes and prognostic factors associated with this condition.

Comparison of Postoperative Outcomes in Colorectal Cancer Patients Undergoing Enhanced Recovery After Surgery (ERAS) vs Non-ERAS: A Retrospective Study Erdiansyah Reza Lesmana; Ida Bagus Budhi Surya Adnyana
Open Access Indonesian Journal of Medical Reviews Vol. 5 No. 4 (2025): Open Access Indonesian Journal of Medical Reviews
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/oaijmr.v5i4.724

Colorectal cancer (CRC) represented a significant global health burden, contributing to approximately 10% of all cancer-related mortality worldwide. While surgery remained the primary treatment for resectable CRC, the postoperative period was often characterized by prolonged recovery and complications. Enhanced Recovery After Surgery (ERAS) protocols emerged as a multimodal approach to mitigate these challenges by attenuating surgical stress, optimizing nutritional status, minimizing opioid consumption, promoting early mobilization, and improving psychological well-being. This study aimed to compare the postoperative outcomes between colorectal cancer patients who underwent surgery with ERAS protocols and those who followed traditional non-ERAS recovery pathways. A retrospective cohort study was conducted at a single academic institution. The study population comprised 201 adult patients who underwent surgical resection for colorectal cancer between January 2020 and December 2024. Patients were categorized into two groups: the ERAS group (n=135), who were managed according to a standardized ERAS protocol, and the non-ERAS group (n=66), who received traditional postoperative care. Statistical analysis involved independent samples t-tests for continuous variables and chi-square tests for categorical variables, with a significance level set at p < 0.05. The ERAS group demonstrated a statistically significant reduction in the mean length of hospital stay compared to the non-ERAS group (7.67 days vs. 8.83 days, p < 0.001). While the ERAS group exhibited slightly higher mean hemoglobin levels postoperatively (11.29 g/dL vs. 11.14 g/dL, p = 0.56), this difference was not statistically significant. Notably, the incidence of postoperative complications was lower in the ERAS group (1 case) compared to the non-ERAS group (4 cases), with a p-value of 0.051, indicating a trend towards significance. In conclusion, the implementation of an ERAS protocol was associated with improved postoperative outcomes in colorectal cancer patients, specifically a significant decrease in the length of hospital stay and a trend towards a lower rate of complications. These findings supported the integration of ERAS pathways into the standard of care for colorectal cancer surgery to enhance patient recovery, potentially reduce healthcare costs, and improve overall patient outcomes.

The Role of Mount Lawu Propolis in Modulating Oxidative Stress and Angiogenic Factors During the Proliferation Phase of Wound Healing Muhammad Apri Rajab; Amru Sungkar; Ida Bagus Budhi Surya Adnyana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 6 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i6.1298

Background: The skin, the body's largest and first-line defense organ, is crucial for protection against environmental aggressors, thermoregulation, fluid balance, immune surveillance, sensory perception, and the intricate process of wound healing. This study investigated the modulatory role of Mount Lawu propolis extract on Malondialdehyde (MDA), a marker of oxidative stress, and Vascular Endothelial Growth Factor (VEGF), a key mediator of angiogenesis, during the critical proliferation phase of wound healing. Methods: A post-test-only control group design experimental study was conducted using twenty-four male rats (Rattus norvegicus). Following the creation of standardized full-thickness skin excision wounds, the rats were divided into four groups (n=6 per group): a control group receiving no propolis, and three treatment groups receiving topical applications of 200mg/kgBB (Group 1), 400mg/kgBB (Group 2), and 800mg/kgBB (Group 3) ethanol propolis extract daily for seven days. MDA and VEGF expression in granulated tissue samples were evaluated using immunohistochemistry and semi-quantitatively scored. Statistical analysis involved the Kruskal-Wallis non-parametric test, followed by the Mann-Whitney test due to non-normal data distribution. Results: MDA levels showed a significant reduction (p<0.05) in Group 2 (400mg/kgBB propolis) compared to the control, indicating a dose-specific antioxidant effect. VEGF expression demonstrated a significant increase (p<0.05) in all propolis-treated groups (Groups 1, 2, and 3) compared to the control, confirming the pro-angiogenic potential of propolis. Notably, the highest VEGF expression was observed in Group 3 (800mg/kgBB propolis). Conclusion: Mount Lawu ethanol propolis extract effectively modulated both oxidative stress and angiogenesis during the proliferation phase of wound healing in this rat model. A 400mg/kgBB/day dose of propolis optimally reduced MDA expression, while propolis at all doses, particularly 800mg/kgBB/day, significantly enhanced VEGF expression. These findings suggest propolis as a promising therapeutic agent to enhance wound healing by mitigating oxidative stress and promoting angiogenesis.

Perioperative Blood Glucose Levels as a Predictor of Hospital Length of Stay in Complicated Appendicitis: A Prospective Cohort Study Trengginas, Satrio Sarwo; Ida Bagus Budhi Surya Adnyana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 6 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i6.1306

Background: Complicated appendicitis presents significant management challenges. Perioperative blood glucose levels are emerging as potential prognostic indicators in surgery. This study aimed to evaluate the correlation between perioperative blood glucose levels and hospital length of stay in patients with complicated appendicitis. Methods: This prospective cohort study was conducted at Dr. Moewardi General Hospital, Indonesia, from October 2020 to October 2021. We enrolled 49 adult patients with complicated appendicitis. Preoperative and postoperative blood glucose levels were measured. The primary outcome was hospital length of stay. Spearman’s correlation and ROC curve analyses were performed. Results: Significant positive correlations were found between hospital length of stay and preoperative blood glucose (r=0.324, p=0.023), postoperative blood glucose (r=0.484, p=0.000), age (r=0.324, p=0.023), and surgical site infection (r=0.360, p=0.011). Postoperative blood glucose showed the strongest correlation. ROC analysis identified a postoperative blood glucose level >123 mg/dL as a cut-off for prolonged hospitalization (>3 days), with 65.2% sensitivity and 65.4% specificity (AUC=0.71). Conclusion: Perioperative blood glucose levels significantly correlate with hospital length of stay in complicated appendicitis, with postoperative levels showing the strongest association. Monitoring postoperative blood glucose may help predict and potentially reduce the duration of hospitalization.

The Efficacy of NeuroAid™ (MLC601) in Modulating NF-κB Expression and Improving Outcomes in Traumatic Brain Injury: A Preclinical Study Dedy Chandra Hariyono; Hanis Setyono; Ida Bagus Budhi Surya Adnyana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 6 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i6.1309

Background: Traumatic brain injury (TBI) represents a significant global health concern, leading to substantial mortality and long-term disability. The intricate pathophysiology of TBI involves primary mechanical damage followed by a cascade of secondary injury events, including neuroinflammation, apoptosis, and oxidative stress. The nuclear factor kappa B (NF-κB) signaling pathway plays a pivotal role in orchestrating the inflammatory response post-TBI and has emerged as a potential therapeutic target. This preclinical study aimed to investigate the efficacy of NeuroAid™ (MLC601), a traditional herbal medicine, in modulating NF-κB expression and improving outcomes in a rat model of TBI. Methods: This study employed a true experimental in vivo design with a post-test only control group. Male Wistar rats (n=18) were randomly divided into two groups: a control group (n=9) subjected to TBI via a weight-drop method, and an experimental group (n=9) subjected to the same TBI procedure followed by intraperitoneal administration of NeuroAid™ (MLC601) at a dose of 2.5 mg/kg body weight at 5 minutes, 8 hours, and 16 hours post-injury. NF-κB expression in brain tissue samples collected 1 hour after the final dose was assessed using immunohistochemistry and quantified by an immunoreactivity score considering both the intensity and percentage of NF-κB expression. Results: Immunohistochemical analysis revealed the presence of NF-κB expression in both the nucleus and cytoplasm of neurons in both the control and experimental groups. While the experimental group treated with NeuroAid™ (MLC601) exhibited a lower average immunoreactivity score (0.93) compared to the control group (1.29), the difference in NF-κB expression between the two groups was not statistically significant (p = 0.122). Conclusion: In this preclinical study using a Wistar rat model of TBI, the administration of NeuroAid™ (MLC601) did not result in a statistically significant reduction in NF-κB expression compared to the untreated control group. Although a trend towards lower NF-κB expression was observed in the NeuroAid™-treated group, further research with larger sample sizes, different dosages, and extended treatment durations is warranted to fully elucidate the potential therapeutic effects of NeuroAid™ (MLC601) in the management of traumatic brain injury.

The Role of Mount Lawu Propolis in Modulating Oxidative Stress and Angiogenic Factors During the Proliferation Phase of Wound Healing Muhammad Apri Rajab; Amru Sungkar; Ida Bagus Budhi Surya Adnyana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 6 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i6.1298

Background: The skin, the body's largest and first-line defense organ, is crucial for protection against environmental aggressors, thermoregulation, fluid balance, immune surveillance, sensory perception, and the intricate process of wound healing. This study investigated the modulatory role of Mount Lawu propolis extract on Malondialdehyde (MDA), a marker of oxidative stress, and Vascular Endothelial Growth Factor (VEGF), a key mediator of angiogenesis, during the critical proliferation phase of wound healing. Methods: A post-test-only control group design experimental study was conducted using twenty-four male rats (Rattus norvegicus). Following the creation of standardized full-thickness skin excision wounds, the rats were divided into four groups (n=6 per group): a control group receiving no propolis, and three treatment groups receiving topical applications of 200mg/kgBB (Group 1), 400mg/kgBB (Group 2), and 800mg/kgBB (Group 3) ethanol propolis extract daily for seven days. MDA and VEGF expression in granulated tissue samples were evaluated using immunohistochemistry and semi-quantitatively scored. Statistical analysis involved the Kruskal-Wallis non-parametric test, followed by the Mann-Whitney test due to non-normal data distribution. Results: MDA levels showed a significant reduction (p<0.05) in Group 2 (400mg/kgBB propolis) compared to the control, indicating a dose-specific antioxidant effect. VEGF expression demonstrated a significant increase (p<0.05) in all propolis-treated groups (Groups 1, 2, and 3) compared to the control, confirming the pro-angiogenic potential of propolis. Notably, the highest VEGF expression was observed in Group 3 (800mg/kgBB propolis). Conclusion: Mount Lawu ethanol propolis extract effectively modulated both oxidative stress and angiogenesis during the proliferation phase of wound healing in this rat model. A 400mg/kgBB/day dose of propolis optimally reduced MDA expression, while propolis at all doses, particularly 800mg/kgBB/day, significantly enhanced VEGF expression. These findings suggest propolis as a promising therapeutic agent to enhance wound healing by mitigating oxidative stress and promoting angiogenesis.

Perioperative Blood Glucose Levels as a Predictor of Hospital Length of Stay in Complicated Appendicitis: A Prospective Cohort Study Trengginas, Satrio Sarwo; Ida Bagus Budhi Surya Adnyana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 6 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i6.1306

Background: Complicated appendicitis presents significant management challenges. Perioperative blood glucose levels are emerging as potential prognostic indicators in surgery. This study aimed to evaluate the correlation between perioperative blood glucose levels and hospital length of stay in patients with complicated appendicitis. Methods: This prospective cohort study was conducted at Dr. Moewardi General Hospital, Indonesia, from October 2020 to October 2021. We enrolled 49 adult patients with complicated appendicitis. Preoperative and postoperative blood glucose levels were measured. The primary outcome was hospital length of stay. Spearman’s correlation and ROC curve analyses were performed. Results: Significant positive correlations were found between hospital length of stay and preoperative blood glucose (r=0.324, p=0.023), postoperative blood glucose (r=0.484, p=0.000), age (r=0.324, p=0.023), and surgical site infection (r=0.360, p=0.011). Postoperative blood glucose showed the strongest correlation. ROC analysis identified a postoperative blood glucose level >123 mg/dL as a cut-off for prolonged hospitalization (>3 days), with 65.2% sensitivity and 65.4% specificity (AUC=0.71). Conclusion: Perioperative blood glucose levels significantly correlate with hospital length of stay in complicated appendicitis, with postoperative levels showing the strongest association. Monitoring postoperative blood glucose may help predict and potentially reduce the duration of hospitalization.

The Efficacy of NeuroAid™ (MLC601) in Modulating NF-κB Expression and Improving Outcomes in Traumatic Brain Injury: A Preclinical Study Dedy Chandra Hariyono; Hanis Setyono; Ida Bagus Budhi Surya Adnyana
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 6 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i6.1309

Background: Traumatic brain injury (TBI) represents a significant global health concern, leading to substantial mortality and long-term disability. The intricate pathophysiology of TBI involves primary mechanical damage followed by a cascade of secondary injury events, including neuroinflammation, apoptosis, and oxidative stress. The nuclear factor kappa B (NF-κB) signaling pathway plays a pivotal role in orchestrating the inflammatory response post-TBI and has emerged as a potential therapeutic target. This preclinical study aimed to investigate the efficacy of NeuroAid™ (MLC601), a traditional herbal medicine, in modulating NF-κB expression and improving outcomes in a rat model of TBI. Methods: This study employed a true experimental in vivo design with a post-test only control group. Male Wistar rats (n=18) were randomly divided into two groups: a control group (n=9) subjected to TBI via a weight-drop method, and an experimental group (n=9) subjected to the same TBI procedure followed by intraperitoneal administration of NeuroAid™ (MLC601) at a dose of 2.5 mg/kg body weight at 5 minutes, 8 hours, and 16 hours post-injury. NF-κB expression in brain tissue samples collected 1 hour after the final dose was assessed using immunohistochemistry and quantified by an immunoreactivity score considering both the intensity and percentage of NF-κB expression. Results: Immunohistochemical analysis revealed the presence of NF-κB expression in both the nucleus and cytoplasm of neurons in both the control and experimental groups. While the experimental group treated with NeuroAid™ (MLC601) exhibited a lower average immunoreactivity score (0.93) compared to the control group (1.29), the difference in NF-κB expression between the two groups was not statistically significant (p = 0.122). Conclusion: In this preclinical study using a Wistar rat model of TBI, the administration of NeuroAid™ (MLC601) did not result in a statistically significant reduction in NF-κB expression compared to the untreated control group. Although a trend towards lower NF-κB expression was observed in the NeuroAid™-treated group, further research with larger sample sizes, different dosages, and extended treatment durations is warranted to fully elucidate the potential therapeutic effects of NeuroAid™ (MLC601) in the management of traumatic brain injury.

Temporal Dynamics of Postoperative Enterocolitis in Hirschsprung Disease: A Comparative Analysis of Earlier Onset After TAERPT versus the Duhamel Procedure Anisa Ika Pratiwi; Nunik Agustriani; Suwardi; Ida Bagus Budhi Surya Adnyana; Henky Agung Nugroho
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 10 No. 1 (2026): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v10i1.1478

Background: Hirschsprung-associated enterocolitis (HAEC) remains the most formidable cause of morbidity in surgically corrected Hirschsprung disease (HD). While the transanal endorectal pull-through (TAERPT) and the Duhamel procedure are standard treatments, a critical knowledge gap exists regarding the timing of postoperative HAEC onset associated with each technique. This study aimed to investigate and compare the temporal dynamics of HAEC presentation following these distinct surgical reconstructions. Methods: This single-center, retrospective cohort study reviewed 64 HD patients who underwent either TAERPT (n=32) or a modified Duhamel procedure (n=32) between January 2022 and January 2023 at a tertiary referral hospital. The primary outcome was the incidence of severe HAEC (HAEC score ≥10). The principal secondary outcome was the time to onset of the first episode of mild-to-moderate HAEC (score <10). Due to the non-normal distribution of onset data, the Mann-Whitney U test was used for statistical comparison. Results: Baseline demographic and clinical characteristics were comparable between the two cohorts. The incidence of severe HAEC was 0% in both the TAERPT and Duhamel groups. All recorded complications were mild-to-moderate and managed non-surgically. A statistically significant and clinically profound difference in the timing of these complications was observed. The median onset of HAEC in the TAERPT group was 6.0 months (Interquartile Range [IQR], 3.0-6.0), which was significantly earlier than the median onset of 8.5 months (IQR, 3.0-24.0) in the Duhamel group (p < 0.001). The mean onset times were 5.50 ± 1.90 months and 16.09 ± 16.33 months, respectively. Conclusion: Although both TAERPT and the Duhamel procedure demonstrated excellent safety profiles regarding severe HAEC, their associated temporal patterns of mild-to-moderate enterocolitis are markedly different. The significantly earlier onset of complications following TAERPT suggests that postoperative surveillance strategies should be procedure-specific, with intensified clinical vigilance during the first postoperative year for TAERPT patients.

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