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Identification of Tropical Marine Diatom Chaetoceros dayaensis CBO from Bokor Island through Morphology and Genetic Marker Analysis Stiawan, Elva; Sutomo, Sutomo; Sapulete, Sherly; Nurachman, Zeily; Rachmayanti, Yanti
Indonesian Journal of Chemical Studies Vol. 1 No. 1 (2022): Indones. J. Chem. Stud., June 2022
Publisher : Indonesian Scholar Society

Microalgae are well-recognized by many researchers working on bioenergy resources due to its capability to produce high amounts of lipids as biodiesel precursors. Diatoms as a group of microalgae that specifically categorized by their silica valve could be more attractive to the researchers because in addition to high-lipid content, their silica features can be also utilized for wider applications, or used for diatoms bioproduct purification. Identification of some diatom species, such as the diatom genus Chaetoceros, as an initial step in diatom studies were difficult to accurately perform because of the unique morphology of silica frustules. Meanwhile, some candidate universal genetic markers of microalgae could also be used for the identification of diatoms with brittle frustules. In this research, we aimed to identify isolate CBO of tropical marine Chaetoceros CBO strain using morphological and genetic approaches. The isolate CBO of Chaetoceros sp. CBO was successfully obtained from sea region of Bokor Island, Kepulauan Seribu, Jakarta, and cultivated in our laboratory as culture stock for further use. Better resolution of the Chaetoceros CBO identification until species level was shown by genetic analysis toward two proposed gene markers for microalgae (rbcL-3P and V4 region of 18S rDNA) rather than by morphological. Specific DNA region for this specimen was found in V4 region of 18S rDNA genetic marker that could be recognized by MspA1I restriction enzyme. Hence, the RFLP (restriction fragment length polymorphism) method could be also used as an initial diagnostic tool for identification of this tropical marine Chaetoceros dayaensis CBO and for stock culture labelling purposes.

Fingerprints, Antioxidant Activity, and Anti-Tyrosinase of Fungus Comb Extract Dewi, Trisni Untari; Diposani, Diva Putri; Rohaeti, Eti; Nandika, Dodi; Karlinasari, Lina; Arinana, Arinana; Santoso, Djoko; Witasari, Lucia Dhiantika; Rachmayanti, Yanti; Batubara, Irmanida
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.346

Fungus comb is a structure made by termite colony Macrotermitinae with lots of potential because it contains secondary metabolites such as steroids, terpenoids, and saponins and compounds containing antioxidant and anti-tyrosinase properties. This study aimed to determine fungus comb extracts' fingerprint pattern, antioxidant capacity, and anti-tyrosinase activity. Thin layer chromatography (TLC) was used to determine fingerprint patterns. The resulting TLC pattern was processed with ImageJ and converted into a densitogram. The fungus comb was extracted with n-hexane and ethyl acetate. TLC results showed that fungus comb extracts contain steroids and terpenoids and have antioxidant properties. The antioxidant capacity assay was carried out with 2,2-diphenyl-1-picrylhydrazyl reagent. N-hexane extract had an antioxidant capacity value of 9.07 mg ascorbic acid equivalent (AAE)/g extract, while ethyl acetate extract had 6.04 mg AAE/g extract. Anti-tyrosinase activity assay was carried out with L-tyrosine and L-3,4-dihydroxyphenylalanine (L-DOPA) as substrates. N-hexane extract had a better anti-tyrosinase activity with IC50 of 1804 µg/mL for L-tyrosine and 6784 µg/mL for L-DOPA.

Photosynthetic efficiency and acid tolerance in Pumiliosphaera acidicola KMJ isolated from a geothermal spring in West Java, Indonesia Kono, Alfredo; Pertiwi, Berliana Gita N.; Farhan, Bahzad Ahmad; Rahmawan, Anhar; Rachmayanti, Yanti; Putri, Rindia M.; Nurachman, Zeily
Acta Biochimica Indonesiana Vol. 8 No. 1 (2025): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.221

Background: Acidophilic microalgae represent a promising yet underexplored resource for biotechnological carbon capture in low-pH environments such as geothermal springs and industrial effluents. However, few strains have been physiologically characterized, and most biotechnologically relevant microalgae remain neutrophilic. Objective: This study aimed to isolate and characterize an acid-tolerant green microalga from a geothermal spring in Kamojang, West Java, Indonesia, and assess its growth, acid tolerance, and photosynthetic performance across a range of inorganic carbon (Ci: CO₂, HCO₃⁻, and CO₃²⁻) concentrations. Methods: Mud samples were enriched in Allen medium under continuous illumination. Isolates were identified via 18S rRNA sequencing and phylogenetic analysis. Growth was tested across pH 2.3 to 7.0 in media acidified with either HCl or H₂SO₄. Photosynthetic performance was evaluated by O₂ evolution under various Ci concentrations. Results: The isolated strain, designated Pumiliosphaera acidicola KMJ, exhibited robust growth from pH 2.3 to 7.0 and showed comparable tolerance to both HCl and H₂SO₄. Morphologically, KMJ displayed compact, spherical green cells (2–5 µm diameter). Oxygen evolution measurements revealed consistently higher photosynthetic rates than Chlamydomonas reinhardtii, particularly under sub-saturating Ci levels, indicating efficient CO₂ assimilation under acidic, low-Ci conditions. To our knowledge, this is the first study to directly quantify Ci-dependent O₂ evolution in an acidophilic chlorophyte. Conclusion: P. acidicola KMJ combines broad acid tolerance with high photosynthetic efficiency, positioning it as a strong candidate for CO₂ capture in acidic environments. Its physiology also provides a basis for future molecular studies into acidophilic CO₂ assimilation.

Fingerprints, Antioxidant Activity, and Anti-Tyrosinase of Fungus Comb Extract Dewi, Trisni Untari; Diposani, Diva Putri; Rohaeti, Eti; Nandika, Dodi; Karlinasari, Lina; Arinana, Arinana; Santoso, Djoko; Witasari, Lucia Dhiantika; Rachmayanti, Yanti; Batubara, Irmanida
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.346

Fungus comb is a structure made by termite colony Macrotermitinae with lots of potential because it contains secondary metabolites such as steroids, terpenoids, and saponins and compounds containing antioxidant and anti-tyrosinase properties. This study aimed to determine fungus comb extracts' fingerprint pattern, antioxidant capacity, and anti-tyrosinase activity. Thin layer chromatography (TLC) was used to determine fingerprint patterns. The resulting TLC pattern was processed with ImageJ and converted into a densitogram. The fungus comb was extracted with n-hexane and ethyl acetate. TLC results showed that fungus comb extracts contain steroids and terpenoids and have antioxidant properties. The antioxidant capacity assay was carried out with 2,2-diphenyl-1-picrylhydrazyl reagent. N-hexane extract had an antioxidant capacity value of 9.07 mg ascorbic acid equivalent (AAE)/g extract, while ethyl acetate extract had 6.04 mg AAE/g extract. Anti-tyrosinase activity assay was carried out with L-tyrosine and L-3,4-dihydroxyphenylalanine (L-DOPA) as substrates. N-hexane extract had a better anti-tyrosinase activity with IC50 of 1804 µg/mL for L-tyrosine and 6784 µg/mL for L-DOPA.

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