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The Effect of Concentration and Immersion Time Disinfectant on Sterilization Aglaonema Hybrid (Pink Katrina) Leaves Budiarni, Kasandra; Hindaningrum, Irni Furnawanthi; Muharromah, Restu Siwi; Kartiman, Roni; Novita, Linda; Hardiani, Lany; Elya, Mardoni; Kubil
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7632

Abstract

Aglaonema sp. is well known as an ornamental plant in Indonesian society. It has leaf with various beautiful patterns. Aglaonema pink katrina is one of the imported hybrids Aglaonema. A method to increase plant yield quickly is tissue culture. The crucial step in plant tissue culture is sterilization. This study aimed to find the best sterilization method by looking at the effect of concentration and immersion time of sodium hypochlorite (NaOCl) as disinfectant to reduce contaminants explant Aglaonema Pink Katrina leaves. In this study, there were 11 groups consisting of positive control, negative control, and immersion with 2,5; 5%; and 10% NaOCl for 1 minute and 3 minutes. Parameters observed included the time of first contamination, percentage of bacterial and fungal contamination, and appearance of explants for 28 days. The DMRT test showed significant differences in all treatments of disinfectant concentration and soaking time.The results show contamination first time occurred in code S0a (negative control), the highest percentage of sterile explants was in code A5, and explants that were still green/fresh were found in codes A5 and A8 after observation.
The Effect of Concentration and Immersion Time Disinfectant on Sterilization Aglaonema Hybrid (Pink Katrina) Leaves Budiarni, Kasandra; Hindaningrum, Irni Furnawanthi; Muharromah, Restu Siwi; Kartiman, Roni; Novita, Linda; Hardiani, Lany; Elya, Mardoni; Kubil
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7632

Abstract

Aglaonema sp. is well known as an ornamental plant in Indonesian society. It has leaf with various beautiful patterns. Aglaonema pink katrina is one of the imported hybrids Aglaonema. A method to increase plant yield quickly is tissue culture. The crucial step in plant tissue culture is sterilization. This study aimed to find the best sterilization method by looking at the effect of concentration and immersion time of sodium hypochlorite (NaOCl) as disinfectant to reduce contaminants explant Aglaonema Pink Katrina leaves. In this study, there were 11 groups consisting of positive control, negative control, and immersion with 2,5; 5%; and 10% NaOCl for 1 minute and 3 minutes. Parameters observed included the time of first contamination, percentage of bacterial and fungal contamination, and appearance of explants for 28 days. The DMRT test showed significant differences in all treatments of disinfectant concentration and soaking time.The results show contamination first time occurred in code S0a (negative control), the highest percentage of sterile explants was in code A5, and explants that were still green/fresh were found in codes A5 and A8 after observation.
ANALISIS STRATEGI DENGAN METODE SWOT DAN QSPM : STUDI KASUS PADA GRIYA COKELAT NGLANGGERAN Muharromah, Restu Siwi; Kismantoroadji, Teguh
Jurnal AGRISEP JURNAL AGRISEP VOL 23 NO 01 2024 (MARCH)
Publisher : Badan Penerbitan Fakultas Pertanian, Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jagrisep.23.01.23-38

Abstract

The purpose of the study is to (1) examine the strategies that currently applied to Griya Cokelat Nglanggeran (2) to design the strategic decisions that can be applied for the development of Griya Cokelat Nglanggeran. This research is a quantitative research with the method of implementation by using descriptive research, taking saturated sampling (census sampling) involving all 13 available respondents. Techniques of collecting data used are interviews, observation, documentation and literature study. Data analysis techniques will be tested with IFE (Internal Factor Evaluation) matrix and EFE (External Factor Evaluation) matrix, IE (Internal-External) matrix, SWOT (Strength, Weakness, Opportunity, Threat) matrix and QSPM (Quantitative Strategic Planning Matrix) to decide the best strategic that can be applied. Based on the data in the processed matrix, the study draws key conclusions that (1) it highlights that the current strategies in Griya Cokelat Nglanggeran, particularly in production and marketing are limited. (2) The strategy applied based on the calculation on the SWOT matrix is the WO (Weakness-Opportunity) Strategy and the right strategy decision to be applied based on the QSPM matrix is a market development strategy based on the QSPM matrix analysis results. Overall, these findings provide valuable insights for the enhancement of Griya Cokelat Nglanggeran's business strategies.