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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Molekul: Jurnal Ilmiah Kimia
Budiarni, Kasandra
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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The Effect of Concentration and Immersion Time Disinfectant on Sterilization Aglaonema Hybrid (Pink Katrina) Leaves Budiarni, Kasandra; Hindaningrum, Irni Furnawanthi; Muharromah, Restu Siwi; Kartiman, Roni; Novita, Linda; Hardiani, Lany; Elya, Mardoni; Kubil
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7632

Abstract

Aglaonema sp. is well known as an ornamental plant in Indonesian society. It has leaf with various beautiful patterns. Aglaonema pink katrina is one of the imported hybrids Aglaonema. A method to increase plant yield quickly is tissue culture. The crucial step in plant tissue culture is sterilization. This study aimed to find the best sterilization method by looking at the effect of concentration and immersion time of sodium hypochlorite (NaOCl) as disinfectant to reduce contaminants explant Aglaonema Pink Katrina leaves. In this study, there were 11 groups consisting of positive control, negative control, and immersion with 2,5; 5%; and 10% NaOCl for 1 minute and 3 minutes. Parameters observed included the time of first contamination, percentage of bacterial and fungal contamination, and appearance of explants for 28 days. The DMRT test showed significant differences in all treatments of disinfectant concentration and soaking time.The results show contamination first time occurred in code S0a (negative control), the highest percentage of sterile explants was in code A5, and explants that were still green/fresh were found in codes A5 and A8 after observation.
The Effect of Concentration and Immersion Time Disinfectant on Sterilization Aglaonema Hybrid (Pink Katrina) Leaves Budiarni, Kasandra; Hindaningrum, Irni Furnawanthi; Muharromah, Restu Siwi; Kartiman, Roni; Novita, Linda; Hardiani, Lany; Elya, Mardoni; Kubil
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7632

Abstract

Aglaonema sp. is well known as an ornamental plant in Indonesian society. It has leaf with various beautiful patterns. Aglaonema pink katrina is one of the imported hybrids Aglaonema. A method to increase plant yield quickly is tissue culture. The crucial step in plant tissue culture is sterilization. This study aimed to find the best sterilization method by looking at the effect of concentration and immersion time of sodium hypochlorite (NaOCl) as disinfectant to reduce contaminants explant Aglaonema Pink Katrina leaves. In this study, there were 11 groups consisting of positive control, negative control, and immersion with 2,5; 5%; and 10% NaOCl for 1 minute and 3 minutes. Parameters observed included the time of first contamination, percentage of bacterial and fungal contamination, and appearance of explants for 28 days. The DMRT test showed significant differences in all treatments of disinfectant concentration and soaking time.The results show contamination first time occurred in code S0a (negative control), the highest percentage of sterile explants was in code A5, and explants that were still green/fresh were found in codes A5 and A8 after observation.
Synthesis and Antimicrobial Activity of Silver N-Methyl Chitosan Kurniasih, Mardiyah; Purwati, Purwati; Dewi, Ratna Stia; Budiarni, Kasandra
Molekul Vol 17 No 2 (2022)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2022.17.2.5059

Abstract

This study synthesized silver N-methyl chitosan (Ag-NMC) and tested it for its antimicrobial and antifungal activity. Ag-NMC was characterized by FTIR, XRD, measured for its molecular weight (MW), solubility, and toxicity. The antimicrobial activity was tested by the agar diffusion method, determining the MIC (Minimum Inhibitory Concentration), MBC (Minimum Bactericidal Concentration) against Staphylococcus aureus and Escherichia coli bacteria, and determining the Minimum Fungicidal Concentration (MFC) against the fungus Candida albicans. The results showed that Ag-NMC had MW, solubility , and LC50 of 555.65 g/mol, 50 mg/mL, 945,492 mg/L, respectively. The diameter of the inhibition zone from the resulting https://www.surreyhillschallenge.com/ diffusion test showed that Ag-NMC had better antimicrobial activity than N-methyl chitosan (NMC) and chitosan. The MIC, MBC, and MFC values of Ag-NMC were always lower than that of NMC and chitosan.
Co-Authors Elya, Mardoni Hardiani, Lany Irni Furnawanthi Hindaningrum Kartiman, Roni Kubil Linda Novita Mardiyah Kurniasih Muharromah, Restu Siwi Purwati Purwati Ratna Stia Dewi