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All Journal Biosaintifika: Journal of Biology & Biology Education Life Science and Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Fitri, Fadillya Maulidilla
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology

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Potential of Therapeutic Curculigo latifolia Extracts on Alloxan-induced Diabetes in a Male Mus muscullus Haryanto, Haryanto; Sutandi, Agus; Kusumawati, Eni; Nurhayati, Sari; Fitri, Fadillya Maulidilla; Nafsi, Ginan; Nuraeni, Sri Wahyuni
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.40498

Abstract

Curculigo latifolia is a herbaceous plant that is abundant on the islands of Java, Sumatra and Kalimantan. C. latifolia has not been well explored. The research to determine the phytochemical content of C. latifolia, to analyze the organoleptic sweetness level of C. latifolia fruit and to analyze the anti-diabetic potential of C. latifolia plant extracts on  diabetic mice. The study was conducted experimentally using  6 treatment levels and 4 replications. Prior to treatment, mice were induced hyperglycemia using alloxan 150 mg/kg WB were induced subcutaneously. The treatment being tested was oral Ethanol crude extract (ECE) for 28 days with 400 mg/kg WB, namely: G1: oral mineral water; G2: glibenclamide ; G3: ECE leaf; G4: ECE root; G5: ECE fruit and G6: ECE tree. Blood sugar levels were measured at 0, 7, 14, 21 and 28 days after oral ECE. Therefore, respondents stated that after consuming the fruit, they had a sweet-tasting and taste-modifying mineral water with a strength of 82.40 ± 8.36%, but the fruit extract did not show any sweet-tasting and taste-modifying. ANOVA results showed that oral ECE administration had a significant effect low on blood sugar levels. the HSD test was carried out with a 95% confidence level. ECE C. latifolia showed positive results on the tests of flavonoids, phenolics, saponins, alkaloids, triterpenoids and tannins. G4 was effective in reducing sugar levels after short time and  G5 for 28 days (long time). C. latifolia have pharmacology effects to lower sugar levels and has taste modifying to sweetness.
Blood Cell Profiles and Metamorphosis of Rice Field Frog (Fejervarya cancrivora) after Heavy Metal Copper (II) Sulfate Exposure Haryanto, Haryanto; Fitri, Fadillya Maulidilla; Nurhayati, Sari; Fadilla, Aghniya Nur Rizka
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.52293

Abstract

Tadpoles represent a critical stage in frog metamorphosis and are particularly susceptible to environmental stressors. Heavy metals, such as copper, are common industrial pollutants that adversely affect aquatic ecosystems. This study aimed to evaluate the effects of copper (II) sulfate on the blood cell profiles and metamorphic development of rice field frog (Fejervarya cancrivora) tadpoles. Experimental treatments included a positive control (mineral water), a negative control (Propylthiouracil / PTU 0.1 gL-1), and exposure to heavy metal of copper (II) sulfate 0.01 mgL-1 (Cu (II)). Each treatment group comprised nine tadpoles, maintained for 14 days under controlled conditions. After 14 days, the survival rate of tadpoles exposed to Cu (II) was 66.67%. Analysis of variance (ANOVA) revealed that Cu (II) exposure significantly impacted blood cell profiles (p < 0.05). Specifically, Cu (II) exposure led to reduced nuclear size, increased immature erythrocytes, and a higher percentage of abnormal erythrocytes. Although metamorphic growth parameters did not differ significantly among treatments (p > 0.05), Cu (II) exposure accelerated skin pigmentation and induced tail abnormalities, including tumor-like formations. These findings demonstrate that Cu (II) is toxic to the blood cells and induces morphological abnormalities in F. cancrivora tadpoles, highlighting the potential ecological risks of copper contamination in aquatic habitats.
EFFECT OF COLCHICINE TREATMENT ON PLANT GROWTH AND FLOWER DEVELOPMENT IN Zinnia elegans Kusumawati, Eni; Haryanto, Haryanto; Wijaya, Nur Iman; Alaudin, Ahmad Muzaki Nurrah-man; Nurhayati, Sari; Fitri, Fadillya Maulidilla
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3112

Abstract

Refugia is a flowering plant that is intended to trap and destroy insects. Colchicine is reported to induce polyploidy in plants. This study aims to determine germination, growth, and refugia performance of colchicine induced Zinnia elegans at different immersion concentrations and times. This research was conducted experimentally with a completely randomized design. The treatment was immersion in colchicine solution at different times duration. Colchicine concentration 0.01% with immersion time of 6,12,24,48, and 72 hours. Colchicine induction with different immersion times showed a decrease in sprouts height (p<0.05), resulting in abnormal sprouts with three leaves and wider leaf area (correlation test r=0.560). The longer the colchicine immersion showed plant height (correlation test r=0.618). Morphologically, control and colchicine induction results were relatively the same, but flowers resulting from colchicine induction had higher flower heights (correlation test r=0.772) and flower diameter (correlation test r=0.815). Induction of colchicine immersion had a different effect on the growth and reproduction performance of Z. elegans.
EFFECT OF COLCHICINE TREATMENT ON PLANT GROWTH AND FLOWER DEVELOPMENT IN Zinnia elegans Kusumawati, Eni; Haryanto, Haryanto; Wijaya, Nur Iman; Alaudin, Ahmad Muzaki Nurrah-man; Nurhayati, Sari; Fitri, Fadillya Maulidilla
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3112

Abstract

Refugia is a flowering plant that is intended to trap and destroy insects. Colchicine is reported to induce polyploidy in plants. This study aims to determine germination, growth, and refugia performance of colchicine induced Zinnia elegans at different immersion concentrations and times. This research was conducted experimentally with a completely randomized design. The treatment was immersion in colchicine solution at different times duration. Colchicine concentration 0.01% with immersion time of 6,12,24,48, and 72 hours. Colchicine induction with different immersion times showed a decrease in sprouts height (p<0.05), resulting in abnormal sprouts with three leaves and wider leaf area (correlation test r=0.560). The longer the colchicine immersion showed plant height (correlation test r=0.618). Morphologically, control and colchicine induction results were relatively the same, but flowers resulting from colchicine induction had higher flower heights (correlation test r=0.772) and flower diameter (correlation test r=0.815). Induction of colchicine immersion had a different effect on the growth and reproduction performance of Z. elegans.
Co-Authors Agus Sutandi Alaudin, Ahmad Muzaki Nurrah-man Fadilla, Aghniya Nur Rizka Haryanto Haryanto Haryanto Haryanto Kusumawati, Eni Nafsi, Ginan Nuraeni, Sri Wahyuni Nurhayati, Sari Wijaya, Nur Iman