Claim Missing Document
Check
Articles

Found 9 Documents
Search

Viability of Lactobacillus bulgaricus and Streptococcus thermophilus in Encapsulated Probiotic Candy With Freeze-Dry Method Agus Sutandi; Haryanto Haryanto; Eni Kusumawati
Scientiae Educatia: Jurnal Pendidikan Sains Vol 11, No 1 (2022): June 2022
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v11i1.10159

Abstract

The level of viable cell count in probiotic candy, based on WHO standard is >106 CFU/ml or >106 CFU/g. Meanwhile, information on the bacterial viability of probiotic candy according to WHO standards is still limited.  The experimental study was conducted to discover the viability of Lactobacillus bulgaricus and Streptococcus thermophilus in encapsulated probiotic candy. Encapsulants consisted of maltodextrin, gum, corn starch, and skim milk. Encapsulation of probiotic bacteria used freeze-dry method. Probiotic candies were stored at room temperature for 14 days in an aerobic condition. The viability of probiotics candy was tested and the results showed significance for each type of encapsulation (p<0.05). The gum and corn starch encapsulations showed bacteria  viability that met WHO standards for functional foods, which ranged from 20,333,333±7,637,626to 31,553,333±2,741,894 CFU/g (>106 CFU/g). The most preferred encapsulant in terms of taste, texture, and aroma was identified as skim milk encapsulation. Further research on the long-term storage of probiotic candy and the viability of probiotic candy bacteria in the gastrointestinal tract (in vivo) is needed.
Potential of Therapeutic Curculigo latifolia Extracts on Alloxan-induced Diabetes in a Male Mus muscullus Haryanto, Haryanto; Sutandi, Agus; Kusumawati, Eni; Nurhayati, Sari; Fitri, Fadillya Maulidilla; Nafsi, Ginan; Nuraeni, Sri Wahyuni
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.40498

Abstract

Curculigo latifolia is a herbaceous plant that is abundant on the islands of Java, Sumatra and Kalimantan. C. latifolia has not been well explored. The research to determine the phytochemical content of C. latifolia, to analyze the organoleptic sweetness level of C. latifolia fruit and to analyze the anti-diabetic potential of C. latifolia plant extracts on  diabetic mice. The study was conducted experimentally using  6 treatment levels and 4 replications. Prior to treatment, mice were induced hyperglycemia using alloxan 150 mg/kg WB were induced subcutaneously. The treatment being tested was oral Ethanol crude extract (ECE) for 28 days with 400 mg/kg WB, namely: G1: oral mineral water; G2: glibenclamide ; G3: ECE leaf; G4: ECE root; G5: ECE fruit and G6: ECE tree. Blood sugar levels were measured at 0, 7, 14, 21 and 28 days after oral ECE. Therefore, respondents stated that after consuming the fruit, they had a sweet-tasting and taste-modifying mineral water with a strength of 82.40 ± 8.36%, but the fruit extract did not show any sweet-tasting and taste-modifying. ANOVA results showed that oral ECE administration had a significant effect low on blood sugar levels. the HSD test was carried out with a 95% confidence level. ECE C. latifolia showed positive results on the tests of flavonoids, phenolics, saponins, alkaloids, triterpenoids and tannins. G4 was effective in reducing sugar levels after short time and  G5 for 28 days (long time). C. latifolia have pharmacology effects to lower sugar levels and has taste modifying to sweetness.
Pelatihan Pembuatan CV ATS Friendly Menggunakan Platform Kinobi Di SMK Plus Nurul Huda Pasirwangi Agustina, Nabila Nur; Hikmah, Wulan Adinda; Andani, Salma Nabila Putri; Firdaus, Muhammad Gibran; Maksum, Muhammad Farid; Akmal, Muhamad; Fadillah, Jihan Hilmi; Sya’diah, Alvi Latifatus; Sulastri, Alnofika Dewi; Alim, Rofi’ul; Haryanto, Haryanto
Jurnal Pengabdian Sosial Vol. 1 No. 11 (2024): September
Publisher : PT. Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/w8y3z026

Abstract

Proses rekrutmen dalam dunia kerja menjadi salah satu hal penting dalam membangun sumber daya manusia profesional. Perusahaan dapat memilih kandidat sesuai dengan yang dibutuhkan, tetapi untuk masuk ke dunia kerja harus mampu beradaptasi, berdaya saing dan membuat CV yang sesuai. Tujuan dari pengabdian masyarakat ini adalah untuk memberi kesempatan siswa SMK Plus Nurul Huda Pasirwangi  dalam mempelajari CV ATS Friendly untuk dibawa ke dunia kerja. Hasil dari kegiatan pengabdian masyarakat ini yaitu Peserta berhasil membuat CV dengan format yang sesuai dengan ATS menggunakan platform Kinobi, hal ini menunjukkan kemampuan teknis para peserta yang diperoleh setelah mengikuti pelatihan, mendapatkan atensi yang besar dari pihak desa dan juga Yayasan, peserta terlibat aktif dalam diskusi, mengajukan pertanyaan, dan mengikuti setiap sesi pelatihan dengan antusiasme yang tinggi, adanya peningkatan pemahaman mengenai konsep CV ATS Friendly serta cara menggunakan platform Kinobi untuk membuat CV.
Pelatihan Pemanfaatan Potensi Pertanian dalam Bidang Bioteknologi Konvensional di Desa Padaasih Kabupaten Garut Jawa Barat Ganni, Aldhi Ya; Yunus, Muhamad; Nurfadillah, Arni Risma; Meisa, Indri Dwi; Haryanti, Indri; Nuralim, Aji; Wahyudin, Wahyudin; Aditya, Nara; Padila, Qurni; Adillah, Nanda Nur; Haryanto, Haryanto
Jurnal Pengabdian Sosial Vol. 1 No. 12 (2024): Oktober
Publisher : PT. Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/6gqnzp76

Abstract

Pelatihan pemanfaatan pertanian dalam bidang bioteknologi konvensional di Desa Padaasih, Kabupaten Garut, Jawa Barat bertujuan untuk meningkatkan pengetahuan dan keterampilan masyarakat desa dalam menggunakan bioteknologi untuk memanfaatkan hasil pertanian secara efektif. Kegiatan ini melibatkan serangkaian sesi pelatihan yang meliputi teori dan praktik tentang aplikasi bioteknologi dalam pengelolaan potensi pertanian dan menjaga lingkungan, seperti pengendalian hama secara,  peningkatan kualitas tanah dengan menggunakan pupuk organik, serta cara pembuatan makanan probiotik. Metode pelatihan dilakukan melalui pelatihan, diskusi kelompok, dan demonstrasi lapangan. Evaluasi menunjukkan bahwa peserta pelatihan mengalami peningkatan pemahaman dan keterampilan dalam menerapkan teknologi bioteknologi konvensional, yang diharapkan dapat meningkatkan produktivitas pertanian dan kesejahteraan ekonomi masyarakat desa. Hasil dari pelatihan ini diharapkan dapat menjadi model bagi desa-desa lain dalam penerapan bioteknologi untuk pengembangan pertanian berkelanjutan.
Weissella paramesenteroides: A Lactic Acid Bacteria Producing Glutathione from Fermented Vegetable Based Keciwis Leaf Luthfia Hastiani Muharram; Haryanto; Wulan Pertiwi; Nelis Hernahadini; Ima Mukaromah
Jurnal Biodjati Vol 10 No 1 (2025): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i1.30589

Abstract

Glutathione (GSH), is one of the essential antioxidants to prevent free radicals and support the immune system. The low level of glutathione is associated with some diseases such as cancer, Alzheimer's, Parkinson's, and AIDS. Lactic acid bacteria (LAB) can produce glutathione. Indonesia, as a mega biodiversity country, has various vegetables and LAB sources that remain underexplored. This research aims to explore LAB-producing glutathione from fermented products derived from Indonesia's typical plant, the keciwis leaf. The method of this research includes sample preparation, lactic acid bacteria isolation, secondary metabolite production with cysteine precursor addition, glutathione analysis by Ellman's assay, identification of 16S rRNA gene of lactic acid bacteria, and glutathione qualitative analysis by HPLC.  Fermented keciwis leaf-based products contain lactic acid bacteria with positive gram characteristics, can produce 0.33 – 0.37 mM extracellular glutathione. Precursor cysteine addition significantly increases glutathione (p<0,05). The selective lactic acid bacteria producing glutathione was identified as belonging to the genus Weissella, with a similarity value of 98.50%, closely related to Weissella paramesenteroides. Extracellular glutathione produced by this culture showed qualitative results on HPLC with a retention time of 6.34 minutes, indicating the presence of the sulfihydryl group. This study identified a new Indonesia source of lactic acid bacteria of Indonesian–origin lactic acid bacteria, specifically Weissella paramesenteroides, as a promising source for glutathione production
MESENCHYMAL STEM CELLS FROM ADIPOSE TISSUE TO TIGHTEN FACIAL SKIN Najah, Adilla Syahsiyatun; Fadila, Aghniya Nur Rizka; Wibawa, Alifah Mustari Mukti; Sulasikin, Almaydha Naim; Salsabila, Aulia Putri; Salsabila, Anbar Rania; Luthfi, Cahya Arsya; Raflianti, Desti; Hakim, Elsya Bayduri; Haryanto
Journal of Stem Cell Research and Tissue Engineering Vol. 9 No. 1 (2025): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v9i1.67461

Abstract

Skin elasticity is an important aspects of facial care. This study aims to explore the potential of mesenchymal stem cells (MSCs) derived from adipose tissue (adipose-derived stem cells) in facial skin tightening and assess their advantages and limitations in clinical applications. The methods used include literature searches from various officially published online sources as well as analysis of research data regarding the process of isolation, culture, differentiation and clinical applications of ADSCs. The main findings show that adipose-derived stem cells (ADSCs) are able to form new collagen, increase skin elasticity, and repair tissue damage due to aging. The culture process involving isolation of cells from adipose tissue and differentiation of the cells into fibroblasts is essential to exploit the regenerative potential of these cells. Although this technology is promising, this study also identified technical and regulatory challenges that need to be overcome, including patient health criteria, safe ADSc harvest procedures, isolation and culture processes that have a high chance of differentiation and post-application care of ADSc therapy. The significance of these findings is that a better understanding of the mechanism of action of ADSCs can make a positive contribution to the development of beauty therapies that are more effective and safer compared to conventional methods that are often unsatisfactory. Additionally, this approach is expected to provide a long-term solution for individuals who wish to maintain a youthful appearance without the risk of significant side effects. Thus, this research can be a step in integrating stem cell technology in the beauty industry, especially for anti-aging treatments.
Cytogenotoxicity Test and Biological Evaluation of Curculigo latifolia Extract with Bioindicators Allium cepa L. var. aggregate Susanti, Susan; Hernahadini, Nelis; Suprapto, Subagio Joko; Haryanto, Haryanto
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.609-614

Abstract

Curculigo latifolia plants have secondary metabolites that can suppress cell division. Compounds that suppress cell division can be used as anticancer drug candidates. This research aims to determine the effect of C. latifolia root extract on cell division and genotoxic effects using bioindicator A. cepa L. var. aggregatum. This study was conducted experimentally with a completely randomized design (CRD) using 6 treatment levels and 5 replications. The treatment levels in this study were K (water), K + (H2O2 300 Mm), K - (H2O2 26 hours + water 46 hours) and a combination of treatments at P1, P2, and P3 with H2O2 immersion (26 hours) and continued immersion of C. latifolia root extract concentrations of 400 µg mL-1, 600 µg mL-1 and 800 µg mL-1 (46 hours). The roots of A. cepa from the soaking treatment of C. latifolia extract were used to make preparations using the squash method. The results of the ANOVA test showed that the administration of C. latifolia root extract had a significant effect on the mitotic index and cell abnormalities (P<0.05). P1 had the most effective value in reducing the mitotic index and cell chromosome abnormalities.
Blood Cell Profiles and Metamorphosis of Rice Field Frog (Fejervarya cancrivora) after Heavy Metal Copper (II) Sulfate Exposure Haryanto, Haryanto; Fitri, Fadillya Maulidilla; Nurhayati, Sari; Fadilla, Aghniya Nur Rizka
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.52293

Abstract

Tadpoles represent a critical stage in frog metamorphosis and are particularly susceptible to environmental stressors. Heavy metals, such as copper, are common industrial pollutants that adversely affect aquatic ecosystems. This study aimed to evaluate the effects of copper (II) sulfate on the blood cell profiles and metamorphic development of rice field frog (Fejervarya cancrivora) tadpoles. Experimental treatments included a positive control (mineral water), a negative control (Propylthiouracil / PTU 0.1 gL-1), and exposure to heavy metal of copper (II) sulfate 0.01 mgL-1 (Cu (II)). Each treatment group comprised nine tadpoles, maintained for 14 days under controlled conditions. After 14 days, the survival rate of tadpoles exposed to Cu (II) was 66.67%. Analysis of variance (ANOVA) revealed that Cu (II) exposure significantly impacted blood cell profiles (p < 0.05). Specifically, Cu (II) exposure led to reduced nuclear size, increased immature erythrocytes, and a higher percentage of abnormal erythrocytes. Although metamorphic growth parameters did not differ significantly among treatments (p > 0.05), Cu (II) exposure accelerated skin pigmentation and induced tail abnormalities, including tumor-like formations. These findings demonstrate that Cu (II) is toxic to the blood cells and induces morphological abnormalities in F. cancrivora tadpoles, highlighting the potential ecological risks of copper contamination in aquatic habitats.
EFFECT OF COLCHICINE TREATMENT ON PLANT GROWTH AND FLOWER DEVELOPMENT IN Zinnia elegans Kusumawati, Eni; Haryanto, Haryanto; Wijaya, Nur Iman; Alaudin, Ahmad Muzaki Nurrah-man; Nurhayati, Sari; Fitri, Fadillya Maulidilla
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3112

Abstract

Refugia is a flowering plant that is intended to trap and destroy insects. Colchicine is reported to induce polyploidy in plants. This study aims to determine germination, growth, and refugia performance of colchicine induced Zinnia elegans at different immersion concentrations and times. This research was conducted experimentally with a completely randomized design. The treatment was immersion in colchicine solution at different times duration. Colchicine concentration 0.01% with immersion time of 6,12,24,48, and 72 hours. Colchicine induction with different immersion times showed a decrease in sprouts height (p<0.05), resulting in abnormal sprouts with three leaves and wider leaf area (correlation test r=0.560). The longer the colchicine immersion showed plant height (correlation test r=0.618). Morphologically, control and colchicine induction results were relatively the same, but flowers resulting from colchicine induction had higher flower heights (correlation test r=0.772) and flower diameter (correlation test r=0.815). Induction of colchicine immersion had a different effect on the growth and reproduction performance of Z. elegans.