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All Journal Journal of Biomedicine and Translational Research MAHESA : Malahayati Health Student Journal Sanus Medical Journal
Sanjaya, Arief Indra
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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The Effect of Increased Glucose Induction on GSH Levels in Insulin Gaussia Luciferase (iGL) Cells Derived from Rat Pancreatic Beta Cells Ardiansah, Fery; Stujanna, Endin Nokik; Sanjaya, Arief Indra; Listiyaningsih, Erlin; Ningsih, Sri Suciati; Ujianti, Irena; Lestari, Dwi Retna
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.19644

Abstract

Background: Prolonged hyperglycaemia can make the pancreatic beta cells work harder and cause fatigue. When this happens, it can trigger oxidative stress reactions, which can produce free radical compounds that can damage pancreatic beta cells. The body compensates by activating protective mechanisms such as the production of antioxidant compounds to reduce the levels of free radicals in the cells. One such compound is glutathione (GSH). Insulin Gaussia Luciferase (iGL) cells are a cell line derived from rat pancreatic β (beta) cells. These cells can be used as a model of oxidative stress in hyperglycaemia to measure GSH levels and there are no studies using iGL cells to measure GSH levels. Therefore, in this study, the iGL cells are used as the object of research.Objective: To investigate the effect of GSH levels on glucose toxicity condition through in vitro experiments on iGL cells.Methods: The study used 5 different glucose concentrations of 11, 16.5, 22, 33, and 44 mM with the addition of iGL cell growth medium exposed for 7 days. We measured the amount of intracellular GSH using a colourimetric method at a wavelength of 405 nm. The analysis used in this study was a one-way ANOVA test. Differences between groups were tested using SPSS.Results: The results of this study showed that there was an increasing trend in total GSH levels on the third and seventh day.Conclusion: there was a daily decrease in GSH/cell in the iGL sample cells exposed to different concentrations of glucose for 7 days. This can be due to an increase in the oxidative stress reactions in the cells, which can lead to a decrease in the levels of antioxidants.
Hematological parameters of COVID-19 patients at Jakarta Pondok Kopi Islamic Hospital Sanjaya, Arief Indra; Martalena, Dewi; Maki, Naoki
Sanus Medical Journal Vol. 6 No. 1 (2024)
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/sanus.v6i1.12668

Abstract

Background: The 2019 Corona Virus Disease (Covid-19) has become a pandemic. As of 15 January 2021, 223 countries have been infected, with a total number of 92,262,621 confirmed cases, and 1,995,037 deaths. In Indonesia, as of the same date, there were 896,642 confirmed cases and 25,767 deaths.1 Diagnosis of COVID-19 is based on real-time reverse transcriptase-polymerase chain reaction (RT-PCR) results on oropharyngeal/nasopharyngeal swab samples.2 A basic hematological examination is also conducted on patients with suspected COVID-19. The results of baseline hematological examinations are thought to be a predictor of the patient being infected with COVID-19 before the RT-PCR results are released. The purpose of this study is to identify the hematological parameters of COVID-19 patients at Pondok Kopi Islamic Hospital in Jakarta (RSIJ PK) in order to provide an overview of the hematological parameters that can be used to predict the possibility of a patient being infected with COVID-19. Methods: The study adopted a cross-sectional approach, using the database on COVID-19 patients at Pondok Kopi Islamic Hospital. Data variables were categorized and described by frequency and percentage. Statistical analysis was conducted using the likelihood ratio test and SPSS (statistical package for the social sciences) version 26. Results: The number of research respondents was 250, with 142 males (56.8%). The majority of participants were between the ages of 40 and 59, at 127 (50.8%). Most patients exhibited a moderate level of clinical severity, at 215 (86%). The three most common comorbidities were hypertension in 82 patients (32.8%), diabetes in 68 patients (27.2%), and chronic kidney disease in 27 patients (10.8%). The dominant hematological parameters were normal blood leukocytes in 157 patients (62.8%), increased Neutrophil/Lymphocyte Ratio (NLR) (>3.13) in 148 patients (59.2%), low Absolute Lymphocyte Count (ALC) (<1500/µL) in 133 patients (53.2%), increased C-Reactive Proteins (CRP) (>10 mg/L) in 171 patients (68.4%), and increased d-dimer (>0.5 µg/mL) in 148 patients (59.2%). There was a significant correlation between age (p 0.028), comorbid hypertension (p 0.002), comorbid diabetes (p 0.011), leukocyte levels (p 0.045), and ALC levels (p 0.025), and the severity of the COVID-19 disease. Conclusions: The majority of COVID-19 patients had normal blood leukocyte levels, increased NLR, low ALC, and increased CRP and d-dimer.
Hubungan Red Cell Distribution Width (RDW) dan Kadar Troponin I Pada Penyakit Kardiovaskuler di RS Islam Jakarta Cempaka Putih Wikanningtyas, Tri Ariguntar; Wahyuni, Tri; Shabariah, Rahmini; Sanjaya, Arief Indra
MAHESA : Malahayati Health Student Journal Vol 4, No 10 (2024): Volume 4 Nomor 10 (2024)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v4i10.15153

Abstract

ABSTRACT Cardiovascular disease (CVD) remains a serious health issue, both in developed and developing countries. According to the World Heart Federation (WHF), global deaths due to CVD surged from 12.1 million in 1990 to 20.5 million in 2021. The World Health Organization (WHO) reported 17.9 million deaths from cardiovascular diseases in 2021. This study aims to investigate the relationship between Red Cell Distribution Width (RDW) and Troponin I levels in cardiovascular disease patients at Jakarta Islamic Hospital (RSIJ) Cempaka Putih from 2021 to 2022. An analytical cross-sectional design was employed from May to August 2023 at RSIJ Cempaka Putih. Variables studied included patient characteristics, hematological laboratory tests (White Blood Cell (WBC), Red Blood Cell (RBC), Platelet (PLT), Hemoglobin, Red Cell Distribution Width-Standard Deviation (RDW-SD), Red Cell Distribution Width-Coefficient of Variation (RDW-CV), and Troponin I), and EKG examinations. The study involved 70 inpatient CVD patients at RSIJ Cempaka Putih in 2021-2022 who met the inclusion and exclusion criteria. Data analysis was performed using Spearman’s Rho correlation test. The results showed no significant correlation between blood parameters (WBC, RBC, PLT, hemoglobin, RDW-SD, and RDW-CV) and troponin I levels. There was no significant correlation between blood parameters (WBC, RBC, PLT, hemoglobin, RDW-SD, and RDW-CV) with troponin I levels. Factors other than blood parameters measured in this study may play a more significant role in determining troponin I levels. Keywords: Red Cell Distribution Width (RDW), Troponin I, Cardiovascular, Hematology  ABSTRAK Penyakit kardiovaskuler masih menjadi masalah kesehatan yang serius, baik   di   negara   maju maupun berkembang. Kematian akibat penyakit kardiovaskular (CVD) melonjak secara global dari 12,1 juta pada tahun 1990 menjadi 20,5 juta pada tahun 2021, menurut World Heart Federation (WHF). Laporan WHO (2021) sebanyak 17,9 juta orang yang telah meninggal karena penyakit kardiovaskuler.  Penelitian ini bertujuan untuk melihat hubungan Red Cell Distribution Width (RDW) dan kadar Troponin I pada penyakit kardiovaskuler di Rumah Sakit Islam Jakarta (RSIJ) Cempaka Putih tahun 2021-2022.  Penelitian analitik dengan desain cross-sectional, dilakukan di RSIJ Cempaka Putih pada Mei-Agustus 2023. Variabel yang diteliti terkait karakteristik pasien penyakit kardiovaskuler (CVD), pemeriksaan laboratorium hematologi (White blood cell (WBC), Red blood cell  (RBC), platelet (PLT), hemoglobin, Red Cell Distribution Width-Standard Deviation (RDW-SD), Red Cell Distribution Width-Coefficient of Variation (RDW-CV), dan Troponin I), serta pemeriksaan EKG. Penelitian melibatkan 70 pasien rawat inap CVD di RSIJ Cempaka Putih pada tahun 2021-2022 yang memenuhi kriteria inklusi dan ekslusi. Analisis data dengan uji korelasi Spearman’s Rho.  Hasil menunjukkan tidak ada hubungan yang signifikan antara parameter darah (WBC, RBC, PLT, hemoglobin, RDW-SD, dan RDW-CV) dengan kadar troponin I.  Tidak terdapat hubungan yang signifikan antara parameter darah (WBC, RBC, PLT, hemoglobin, RDW-SD, dan RDW-CV) dengan kadar troponin I. Faktor-faktor selain dari parameter darah yang diukur dalam penelitian ini mungkin lebih berperan dalam menentukan kadar troponin I. Kata Kunci: Red Cell Distribution Width (RDW), Troponin I, Kardiovaskuler, Hematologi
Co-Authors Ardiansah, Fery Dewi Martalena ERLIN LISTIYANINGSIH Lestari, Dwi Retna Maki, Naoki Shabariah, Rahmini Sri Suciati Ningsih Stujanna, Endin Nokik tri wahyuni Ujianti, Irena Wikanningtyas, Tri Ariguntar