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All Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Indonesian Journal of Halal Research Kieraha Medical Journal
Nikmatullah, Nurul Azmah
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Religion Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Medicine & Pharmacology Nursing Public Health Other

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Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time Sunaryo, Hadi; Wirman, Adia Putra; Permanasari, Etin Diah; Nikmatullah, Nurul Azmah; Lestari, Dian; Nurjanah, Desi
Indonesian Journal of Halal Research Vol. 5 No. 2 (2023): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v5i2.21325

Abstract

DNA (deoxyribonucleic acid) extraction method is the process of separating DNA from the sample. In this process, the DNA obtained must be protected from contamination by RNA, carbohydrates, lipids, and proteins. Contamination of RNA, carbohydrates, lipids, and proteins can increase DNA purity. DNA purity was measured using a NanoDrop 2000 spectrophotometer measured by the absorbance ratio at 260 nm and 280 nm wavelengths. Good quality DNA will have an A260/A280 ratio of 1.7-2.0 and a concentration > 0.03 pg. This study aimed to obtain the appropriate DNA extraction method for fresh meat samples (a mixture of rat and chicken meat). This research consisted of two stages: the DNA extraction stage using the Progenus EasyFast™ Extraction Kit for Meat Products and the amplification stage using the EASYFAST™ Rat Detection Kit. This study used 16 samples of a mixture of rat meat and chicken with concentrations of rat meat: 5, 10, 15, and 20%. At the extraction stage, the incubation time was optimized for 15, 30, 45 minutes, and 1 hour. The results showed that the one hour incubation had a lowest CT value in the results of PCR amplification.
Efektifitas Larutan ZnSO4 33% dan Sukrosa 33% Pada Pemeriksaan Soil Transmitted Helminth Menggunakan Metode Flotasi Sentrifugasi Nikmatullah, Nurul Azmah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 2 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i2.18289

Abstract

Soil-Transmitted Helminth (STH) infection is an infection caused by Ascaris lumbricoides worms, Ancylostoma duodenale worms, Necator americanus worms, and Trichuris trichiura worms. Transmission of infection occurs through soil media containing feces contaminated with STH worm eggs. STH worm infection can be identified by qualitative and semi-quantitative stool examination. Stool qualitative examination consists of the direct slide method (native), the flotation method, and the sedimentation method. This study aims to see the effectiveness of 33% ZnSO4  solution and 33% sucrose solution in examining STH worm eggs in the centrifugation flotation method. This study began by collecting feces samples; then the samples were identified by using the flotation centrifugation method. The solutions used in this method were 33% ZnSO4 solution and 33% sucrose solution. The data obtained were analyzed by using the Mann-Whitney test. There were 35 stool samples identified. The examination results obtained 13 positive samples of Ascaris lumbricoides worm eggs in 33% ZnSO4  solution and 1 positive sample in 33% sucrose solution, and the results of the Mann Whitney test obtained Asymp. Sig <0.05. Therefore, there is a significant difference between the 33% ZnSO4 solution and the 33% sucrose solution. In conclusion, the most effective solution used in the flotation method is 33% ZnSO4.   Keywords: Soil-Transmitted Helminth Infection, Centrifugation Flotation, 33% ZnSO4 Solution, 33% Sucrose Solution
PREVALENSI JENIS INFEKSI GENITALIA PADA IBU HAMIL TRIMESTER III DI KECAMATAN JATINEGARA Purbasari, Euis; Erikardo, Oktadio; Nikmatullah, Nurul Azmah; Wirman, Adia Putra
Kieraha Medical Journal Vol 6, No 2 (2024): KIERAHA MEDICAL JOURNAL
Publisher : Fakultas Kedokteran, Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/kmj.v6i2.8931

Abstract

Buruknya perilaku personal hygiene berkaitan dengan permasalahan organ reproduksi wanita, ditandai dengan keputihan patologis. Keputihan patologis sangat rentan terjadi pada ibu hamil dikarenakan peningkatan hormon estrogen dapat menyuburkan pertumbuhan mikroorganime merugikan di dalam vagina. Pertumbuhan mikroorganime mengakibatkan kerusakan epitel sehingga pH vagina semakin meningkat, serta didukung kondisi organ reproduksi yang terletak di area tertutup dan berlipat sehingga mudah berkeringat, lembap, dan kotor. Hal ini akan menyebabkan mikroorganisme tumbuh semakin masif. Penelitian ini bertujuan untuk mengetahui prevalensi jenis infeksi genitalia pada ibu hamil sebagai acuan upaya pencegahan dan penanggulangannya. Penelitian ini menggunakan teknik random sampling dengan jumlah sampel 100 orang ibu hamil terinfeksi genitalia. Penentuan infeksi genitalia didasarkan hasil rekam medis pasien. Hasil penelitian menunjukkan prevalensi paling tinggi terjadinya infeksi genitalia pada ibu hamil disebabkan oleh Vaginosis Bakterialis (VB) sebesar 54%, koinfeksi VB dan Candida albicans 30%, Candida albicans 11%, dan Trichomonas vaginalis 5%. Adanya data prevalensi ini, kami berharap unit kesehatan terkait dapat melakukan upaya preventif sehingga menekan angka kejadian infeksi genitalia pada ibu hamil dan melaksanakan pemeriksaan skrining secara rutin.
Co-Authors Adia Putra Wirman Adia Putra Wirman, Adia Putra DIAN LESTARI Etin Diah Permanasari Hadi Sunaryo Nurjanah, Desi Oktadio Erikardo Purbasari, Euis