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All Journal Pharmaciana: Jurnal Kefarmasian Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan BIOEDUSCIENCE JSFK (Jurnal Sains Farmasi & Klinis) Indonesian Journal of Halal Research Abdimasku : Jurnal Pengabdian Masyarakat Mejuajua Journal of Halal Science and Research Jurnal Pengabdian Masyarakat Bangsa Jurnal Agroteknologi Merdeka Pasuruan
Etin Diah Permanasari
Magister Ilmu Farmasi, Sekolah Pascasarjana, Universitas Muhammadiyah Prof DR HAMKA, Jalan Warung Djati, Pancoran, Jakarta Selatan, Indonesia, 12740; Pusat Kajian Halal, Universitas Muhammadiyah Prof DR HAMKA, Jalan Delima II, Duren Sawit, Jakarta Ti
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Edukasi Perilaku Hidup Bersih Sehat dan Pembagian Masker, Handsanitizer kepada Warga Jatisari Fitria Nugrahaeni; Etin Diah Permanasari
ABDIMASKU : JURNAL PENGABDIAN MASYARAKAT Vol 4, No 1 (2021): Januari 2021
Publisher : LPPM UNIVERSITAS DIAN NUSWANTORO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33633/ja.v4i1.162

Abstract

Perilaku Hidup bersih dan sehat (PHBS) merupakan langkah yang harus diaplikasikan untuk mencapai derajat kesehatan yang baik bagi setiap orang. Masyarakat di RT 01 RW 02 Jatisari Bekasi merupakan tempat yang masyarakatnya masih kurang pengetahuan dan informasi sehingga masalah kesehatan masyarakat masih menjadi masalah yang cukup besar. Selain itu akhir-akhir ini sedang terjadi pandemi virus korona yang berbahaya bagi tubuh. Sehingga sangat dibutuhkan edukasi mengenai PHBS dan pencegahan penyakit korona serta penyakit lainnya salah satunya dengan menggunakan masker dan rajin cuci tangan dan kalau di luar jangan lupa menggunakan handsanitizer. Pengabdian ini bertujuan untuk meningkatkan pengetahuan, kesadaran, kemauan dan kemampuan masyarakat untuk melakukan PHBS dalam meningkatkan mutu hidup serta meningkatkan taraf kesehatan masyarakat. Metode yang digunakan adalah memberikan kuesioner dan penyuluhan. Kuesioner diberikan 1 hari sebelum pelaksanaan kegiatan dan setelah pelaksanaan kegiatan kepada 55 responden dengan tetap jaga jarak. Hasil kuesioner digunakan untuk mengetahui pemahaman masyarakat tentang PHBS dan sebagai indikator keberhasilan dari kegiatan. Penyuluhan disampaikan dengan tema PHBS, cara cuci tangan yang benar, dan cara memakai masker yang benar. Selain itu dilakukan pembagian masker dan handsanitizer kepada warga. Hasil penyuluhan menunjukkan terdapat peningkatan yang signifikan pemahaman masyarakat tentang masalah tersebut dari 18,18% menjadi 98,18%. Berdasarkan hasil ini diharapkan masyarakat dapat menerapkan pengetahuan tersebut dengan benar demi mewujudkan derajat kesehatan masyarakat yang setinggi-tingginya.
The Correlation Between Nutritional Status, Age, and Gender on the Incidence of Soil-Transmitted Helminth Infections Nikmatullah, Nurul Azmah; Masyhuda, Irmawati; Permanasari, Etin Diah; Rahmadilla, Chintya
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 17 No 2 (2023): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v17i2.6896

Abstract

Soil-transmitted helminth (STH) infections most commonly infect preschool and school-age children. The prevalence of STH infection remains high, i.e., 73 million children are infected in Indonesia. The most commonly infecting worms are roundworms (Ascaris lumbricoides), whipworms (Trichuris trichiura), and hookworms (Ancylostoma duodenale and Necator americanus). STH infection is transmitted through soil contaminated by STH eggs or larvae. Personal hygiene, availability of clean water, and defecation behavior are factors in the development of STH infection. Prolonged infection can cause malnutrition, anemia, and slow growth. This study aims to examine the association between nutritional status, age, and gender on the incidence of STH infection. This research is a descriptive-analytical study with a cross-sectional approach. Fifteen (42.86%) of 35 preschool and school-age children's stool samples were positive for Ascaris lumbricoides eggs. The nutritional status of the children was grouped into underweight, normal, and overweight. The body mass index (BMI) calculation resulted in 65.71% (23/35) underweight, 31.43% (11/35) normal, and 2.86% (1/35) overweight children. The Spearmen test shows that there is no significant correlation between nutritional status (p=0.517), age (p=0.614), and gender (p=0.637) on STH infection.
Sosialisasi dan Edukasi Penyakit Infeksi Saluran Pernapasan di Kecamatan Menteng, DKI Jakarta Permanasari, Etin Diah; Nugrahaeni, Fitria; Rahmadini, Nuriza; Zakka, Gulsyaniraz Qadhi; Chandra, Ady; Bella, Careninda Ayu
Mejuajua: Jurnal Pengabdian pada Masyarakat Vol. 4 No. 1 (2024): Agustus 2024
Publisher : Yayasan Penelitian dan Inovasi Sumatera (YPIS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52622/mejuajuajabdimas.v4i1.160

Abstract

ISPA (Infeksi Saluran Pernapasan Akut) merupakan penyakit dengan angka morbiditas dan mortalitas yang tinggi di Indonesia. ISPA menjadi penyebab kematian terbesar pada post-neonatal dan balita di Indonesia. DKI Jakarta merupakan salah satu dari tiga provinsi dengan kasus ISPA tertinggi di Indonesia. Tujuan dari pengabdian masyarakat ini adalah sebagai bagian dari tindakan pencegahan ISPA dengan mensosialisasikan pengetahuan dan cara pengobatan ISPA melalui diskusi dan demo pembuatan obat ISPA dari bahan herbal. Metode yang dilakukan adalah penyuluhan, demo praktek pembuatan obat herbal, diskusi, dan tanya jawab yang dihadiri oleh 28 peserta ibu-ibu PKK RW 07 Kelurahan Pegangsaan, Kecamatan Menteng, DKI Jakarta. Hasil korelasi (r) data pre-test dan post-test dengan menggunakan uji Paired Sample T-Test menunjukkan nilai 0.001 (p<0.05) bahwa terdapat korelasi antara pre-test dan post-test. Hasil analisis perbedaan data pre-test dan post-test menunjukkan nilai 0.000 (p<0.05), sehingga dapat disimpulkan terdapat perbedaan signifikan dan peningkatan pengetahuan yang nyata antara hasil pre-test dan post-test. Dari kegiatan ini dapat disimpulkan bahwa pemberian edukasi berupa sosialisasi dan edukasi dapat efektif meningkatkan pengetahuan masyarakat tentang ISPA.
Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time Sunaryo, Hadi; Wirman, Adia Putra; Permanasari, Etin Diah; Nikmatullah, Nurul Azmah; Lestari, Dian; Nurjanah, Desi
Indonesian Journal of Halal Research Vol 5, No 2 (2023): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v5i2.21325

Abstract

DNA (deoxyribonucleic acid) extraction method is the process of separating DNA from the sample. In this process, the DNA obtained must be protected from contamination by RNA, carbohydrates, lipids, and proteins. Contamination of RNA, carbohydrates, lipids, and proteins can increase DNA purity. DNA purity was measured using a NanoDrop 2000 spectrophotometer measured by the absorbance ratio at 260 nm and 280 nm wavelengths. Good quality DNA will have an A260/A280 ratio of 1.7–2.0 and a concentration > 0.03 pg. This study aimed to obtain the appropriate DNA extraction method for fresh meat samples (a mixture of rat and chicken meat). This research consisted of two stages: the DNA extraction stage using the Progenus EasyFast™ Extraction Kit for Meat Products and the amplification stage using the EASYFAST™ Rat Detection Kit. This study used 16 samples of a mixture of rat meat and chicken with concentrations of rat meat: 5, 10, 15, and 20%. At the extraction stage, the incubation time was optimized for 15, 30, 45 minutes, and 1 hour. The results showed that the one hour incubation had a lowest CT value in the results of PCR amplification.
DNA-based detection of Rat in the meatballs product using a real-time polymerase chain reaction method Permanasari, Etin Diah; Sunaryo, Hadi; Wirman, Adia Putra; Rahmadini, Nuriza; Aggasy, Savira Yustinah; Nikmatullah, Nurul Azmah
Pharmaciana Vol. 14 No. 3 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i3.27850

Abstract

The meat-based products are highly susceptible to counterfeiting, primarily due to high consumer demand of meat derivative products, such as meatballs. This demand creates opportunities for food fraud by specific industries, including adulterating meat with non-halal species, such as rats. This research aimed to detect rat meat contamination in meatball samples from the Indonesian local market using Real-time Polymerase Chain Reaction (RT-PCR). The RT-PCR amplification involved an initial denaturation step at 95°C for 3 minutes, followed by denaturation at 95°C for 15 seconds, and annealing/extension at 60°C for 1 minute. The rat-specific probe primer included in the kit produced an increasing curve in the External Positive Control (EPC) with a Ct value of 27.22, and no amplification occurred in the Negative Control (NTC). The analysis of 30 samples from meatball vendors yielded negative results, as there was no increase in the FAM (rat) curve, indicating that none of the meatballs were contaminated with rat DNA.
Total Flavonoid Content and Antioxidant Properties of Different Extraction Methods of Red Spinach Leaf (Amaranthus tricolor L.) Permanasari, Etin Diah; Amalia, Mega Putri Rizky; Susilo, Susilo; Prastiwi, Rini
JSFK (Jurnal Sains Farmasi & Klinis) Vol 11 No 1 (2024): J Sains Farm Klin 11(1), April 2024
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.11.1.17-24.2024

Abstract

Red spinach leaf (Amaranthus tricolor L.) has been well-proven to contain flavonoids that exhibit antioxidant activities. In this study, a comparison between maceration and ultrasonic methods has been carried out to evaluate the effect of these two methods on the level of red spinach leaf flavonoid. In this study, for maceration 1, 2, and 3 days while for the ultrasonic method 10-, 20-, and 30-minutes time duration have been used. The ratios of material and solvent were 1:5 and 1:10. The flavonoid content was determined with the help of a spectrophotometer at a maximum wavelength of 436.80 nm. The antioxidant activity was evaluated by the DPPH method to obtain the IC50. The result of the study revealed that the highest flavonoid content of 4.27 mgQE/g and antioxidant activity (IC50) of 110.47 ppm were obtained by an ultrasonic under conditions of 1:10 ratio and 30-minute extraction time. Results of the study suggested a significant difference (p<0,05) in the isolation of total flavonoid contents and antioxidant activities in the maceration and ultrasonic. In these two methods, ultrasonic was found to be more efficient than maceration because of minimum time, the highest flavonoid content and antioxidant activity could be obtained.
Optimization of DNA Extraction Methods in Fresh Meat (Rat and Chicken Meat) based on Incubation Time Sunaryo, Hadi; Wirman, Adia Putra; Permanasari, Etin Diah; Nikmatullah, Nurul Azmah; Lestari, Dian; Nurjanah, Desi
Indonesian Journal of Halal Research Vol. 5 No. 2 (2023): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v5i2.21325

Abstract

DNA (deoxyribonucleic acid) extraction method is the process of separating DNA from the sample. In this process, the DNA obtained must be protected from contamination by RNA, carbohydrates, lipids, and proteins. Contamination of RNA, carbohydrates, lipids, and proteins can increase DNA purity. DNA purity was measured using a NanoDrop 2000 spectrophotometer measured by the absorbance ratio at 260 nm and 280 nm wavelengths. Good quality DNA will have an A260/A280 ratio of 1.7-2.0 and a concentration > 0.03 pg. This study aimed to obtain the appropriate DNA extraction method for fresh meat samples (a mixture of rat and chicken meat). This research consisted of two stages: the DNA extraction stage using the Progenus EasyFast™ Extraction Kit for Meat Products and the amplification stage using the EASYFAST™ Rat Detection Kit. This study used 16 samples of a mixture of rat meat and chicken with concentrations of rat meat: 5, 10, 15, and 20%. At the extraction stage, the incubation time was optimized for 15, 30, 45 minutes, and 1 hour. The results showed that the one hour incubation had a lowest CT value in the results of PCR amplification.
Antibacterial activity against Lactobacillus acidophilus and Streptococcus mutans from endophytic mold of binahong leaf (Anredera cordifolia (Ten.) Steenis) Permanasari, Etin Diah; Roinaldo, Roinaldo
Jurnal Agroteknologi Merdeka Pasuruan Vol 9, No 1 (2025): JUNI 2025
Publisher : Universitas Merdeka Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51213/jamp.v9i1.125

Abstract

Endophytic microbe is usually used as a natural source to explore novel metabolites which has therapeutic activities. This research’s aim is to determine the antibacterial activity of endophytic molds which grow in the tissue of binahong leaf (Anredera cordifolia (Ten.) Steenis) to resist Lactobacillus acidophilus and Streptococcus mutans. Four endophytic mold isolates were obtained, which were KTS1, KTS2, KTS3, and KTS4. The screening of antibacterial activities for L. acidophilus and S. mutans was carried out from the supernatant of each isolate, and the highest inhibition was obtained by KTS1 with the inhibition diameter of 2,82 mm for L. acidophilus and 2,89 mm for S. mutans. The grading extraction with n-hexane, ethyl acetate, and methanol was used to extract the supernatant. The microdilution method was used to determine the antibacterial activity by measuring the absorbances with a microplate reader at a 595 nm of wavelength. The respective of IC50 of KTS1 in the ethyl acetate (KTS1EA) and methanol (KTS1ME) against L. acidophilus were 9007,80 µg/ml and 10119,45 µg/ml. The relative potency of KTS1EA and KTS1ME to erythromycin against L. acidophilus were 0,0023 and 0,0020 times as respectively. The respective of relative potency of KTS1EA and KTS1ME to erythromycin against S. mutans were 0,0032 and 0,0021 times. However, the IC50 of KTS1EA and KTS1ME against S. mutans were 5878,03 µg/ml and 8928,75 µg/ml as respectively.
Edukasi Pemanfaatan Ekstrak Bekatul sebagai Antioksidan dalam Sediaan Masker Clay Kepada Siswa SMK Farmasi Swadaya Global School Nugrahaeni, Fitria; Amalia, Anisa; Permanasari, Etin Diah; Nining, Nining; Gantini, Sri Nevi
Jurnal Pengabdian Masyarakat Bangsa Vol. 3 No. 4 (2025): Juni
Publisher : Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/jpmba.v3i4.2467

Abstract

Bekatul merupakan hasil sampingan penggilingan padi yang kaya akan antioksidan alami, seperti vitamin E dan asam lemak esensial, yang bermanfaat untuk kesehatan kulit. Pemanfaatannya sebagai bahan dasar dalam formulasi masker clay tidak hanya menjadi alternatif perawatan kulit yang lebih alami dan ekonomis, tetapi juga mendukung pengelolaan limbah pertanian menjadi produk bernilai tambah. Kegiatan Pengabdian kepada Masyarakat (PkM) ini bertujuan untuk meningkatkan pengetahuan siswa SMK Farmasi Swadaya Global School mengenai pemanfaatan bahan alam, khususnya bekatul merah, dalam pembuatan sediaan kosmetika. Metode pelaksanaan mencakup tahap persiapan, pemberian edukasi, serta evaluasi melalui pre-test dan post-test. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan yang signifikan, dengan rata-rata nilai pre-test sebesar 57,60 dan post-test sebesar 80,00. Analisis statistik menggunakan Paired Sample T-Test menunjukkan nilai signifikansi sebesar 0,000 (< 0,05), yang mengindikasikan bahwa pemberian materi berdampak nyata terhadap peningkatan pengetahuan peserta. Data ini memperkuat bukti bahwa edukasi mengenai pemanfaatan ekstrak bekatul merah sebagai antioksidan alami efektif dalam meningkatkan pemahaman siswa terhadap penggunaan bahan alam dalam kosmetika. Kegiatan ini juga berperan dalam mendorong pola hidup sehat, pengembangan inovasi lokal, serta mengurangi ketergantungan pada produk kosmetik berbahan kimia sintetis. Secara keseluruhan, program ini berhasil mendorong kesadaran siswa akan pentingnya pemanfaatan sumber daya alam secara berkelanjutan dalam bidang farmasi dan kecantikan
Exopolysaccharides production by Lactobacillus fermentum under different growth conditions in coconut water medium Permanasari, Etin Diah; Wahyudi , Priyo; Fauziah, Fuji; Robertlee, Jekson
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/13749

Abstract

Background: Exopolysaccharides (EPS) production gain a lot of attention over recent decades, because EPS can provide beneficial effects, not only on the industrial application but also on the health sector. The understanding of the optimal condition for EPS production will increase the productivity of EPS and can develop EPS with desirable properties. The factors affected EPS production are additional of sugar concentration, temperature, fermentation time, and others. The current work aimed to optimize the utilization of a byproduct leftover of coconut water on the EPS production from Lactobacillus fermentum. Methods: The EPS synthesis were analyzed under various growth conditions in coconut water such as additional of sucrose concentration and incubation times. EPS production of Lactobacillus fermentum was performed by adding 1%, 2%, and 3% of sucrose and 12, 24, 36, and 48 h of incubation periods. The obtained data were analyzed statistically using a two-ways ANOVA test. Results: The EPS production increased as the sucrose concentration and incubation time were increased. The optimal production was found to be in the media supplemented with 3% sucrose and 48 h of incubation period, which gave 12.613 g/L of EPS production. Conclusions: Media of coconut water is suitable for EPS production by Lactobacillus fermentum. Under 3% of additional sucrose concentration and 48 h of incubation time, it produced a larger number of EPS compare to other conditions.
Co-Authors Adia Putra Wirman Aggasy, Savira Yustinah Amalia, Mega Putri Rizky Anisa Amalia Bella, Careninda Ayu Chandra, Ady DIAN LESTARI Fatmawati, Sofia Fauziah, Fuji Firmanto, Johan Dwi Fitria Nugrahaeni Gantini, Sri Nevi Hadi Sunaryo Hariyanti Hariyanti Masyhuda, Irmawati Nikmatullah, Nurul Azmah Nining, Nining Nugrahaeni, Fitria Nurjanah, Desi Nurul Azmah Nikmatullah Pamungkas, Septiana Tri Rahmadilla, Chintya Rahmadini, Nuriza Rini Prastiwi Robertlee, Jekson Roinaldo, Roinaldo Sari, Retno Lia Susilo Susilo Wahyudi , Priyo Zakka, Gulsyaniraz Qadhi