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All Journal Jurnal Ilmiah Kesehatan Delima Al-Hayat: Natural Sciences, Health & Environment Journal
Deya Adiby Nabillah
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Education Environmental Science Health Professions Materials Science & Nanotechnology Nursing Public Health

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Analisis Antioksidan Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) Menggunakan Metode DPPH (2,2 – Diphenyl – 1 – picrilhydrazil) Udin, Baha; Deya Adiby Nabillah; Sofi Nurmay Stiani; Fifih Lutfiyah
Jurnal Ilmiah Kesehatan Delima Vol. 6 No. 1 (2023): Jurnal Ilmiah Kesehatan Delima
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat, Sekolah Tinggi Ilmu Kesehatan Salsabila Serang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60010/jikd.v6i1.86

Abstract

Tujuan: Untuk mengetahui kekuatan antioksidan ekstrak etanol kulit buah manggis (Garcinia mangostana L. ) dibandingkan vitamin C sebagai kontrol positif. Metode: Studi telah menguji sifat antioksidan ekstrak etanol kulit buah manggis (Garcinia mangostana L.) yang diekstraksi melalui proses maserasi menggunakan spektrofotometri UV-Vis untuk menganalisis aktivitas antioksidan dengan menggunakan metode DPPH (2,2-diphenyl-1-picrilhydrazil). Kemampuan antioksidan ditentukan dengan absorbansi larutan DPPH mengalami penurunan setelah dilakukan penambahan ekstrak. Hasil dan Pembahasan: Kontrol positif pada penelitian ini, yaitu vitamin C. Hasil analisis menunjukkan bahwa pada menit ke-20, ekstrak etanol kulit buah manggis memiliki IC50 mencapai 47,73 ppm, sedangkan vitamin C memiliki nilai IC50 sebesar 26,58 ppm. Hasil uji kuantitatif senyawa metabolit ekstrak kulit manggis salah satunya positif mengandung senyawa flavonoid, yang berfungsi sebagai antioksidan sehingga mampu meredam radikal bebas dengan cara mendonorkan satu atom hidrogennya. Hasil ini menunjukkan bahwa ekstrak etanol kulit buah manggis (Garcinia mangostana L.) memiliki kemampuan antioksidan untuk menangkal radikal bebas DPPH.
Uji Anti Inflamasi Kombinasi Ekstrak Rimpang Kencur (Kaempferia Galanga L.) dan Daun Salam (Syzygium Polyanthum) pada Tikus Putih Galur Sprague Dawley yang Diinduksi Karagenan Elis Wildayani; Deya Adiby Nabillah; Syilvi Adini
Al-Hayat: Natural Sciences, Health & Environment Journal Vol. 3 No. 1 (2025): Al-Hayat: Natural Sciences, Health & Environment Journal 
Publisher : Institut Agama Islam Nasional Laa Roiba Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47467/alhayat.v3i1.9282

Abstract

Inflammation is the body's response to tissue injury, which is usually caused by physical trauma, harmful chemicals, or microorganisms. Kencur rhizomes and bay leaves have been proven effective as anti-inflammatories. The purpose of this study is to determine the anti-inflammatory effect of a combination of kencur rhizome extract and bay leaves in reducing the volume of 1% carrageenan-induced rat foot edema. The research method is a laboratory experiment using 24 Wistar Sprague dawley male white rats with 1% carrageenan induction. The test materials used were KNR (1% carrageenan), KNT (CMC-Na 0,5%), KD1 (50 : 50 mg/kg BB), KD2 (150 : 250 mg/kg BB), KD3 (250 : 75 mg/kg BB), and KPT (diclofenac sodium) ( mg/kg BB). The test materials were administered orally one hour before induction with 0.2 mL of 1% carrageenan. Foot volume measurements were taken every hour for six hours after carrageenan induction. The results showed that the 150 : 250 mg/kg dose combination group could inhibit the onset of inflammation by 89%; the 250 : 75 mg/kg dose combination group could inhibit it by 75%; and the 50 : 50 mg/kg dose combination group could inhibit it by 56%. In the positive control group (diclofenac sodium), the percentage of inflammation inhibition was 60%. Therefore, it can be concluded that the higher the concentration of kencur rhizome extract and bay leaves, the greater the decrease in anti-inflammatory activity. The combination of kencur rhizome extract and bay leaves at a dose of 150 : 250 mg/kgBB is an effective dose for inhibiting edema, as evidenced by the results of this study.
Co-Authors Baha Udin Elis Wildayani Fifih Lutfiyah Stiani, Sofi Nurmay Syilvi Adini