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All Journal Veterinary Biomedical and Clinical Journal Jurnal Medika Veterinaria
Retno Lastuti, Nunuk Dyah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Medika Veterinaria

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Molecular Detection of Second Internal Transcribed Spacer (ITS-2) Encoding Gene Sarcoptes scabiei of Stray Cat from Several Market in Bondowoso Rusdiana, Nur; Wibawati, Prima Ayu; Retno Lastuti, Nunuk Dyah; Amukti, Pandu Tokoh
Jurnal Medika Veterinaria Vol 19, No 2 (2025): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v19i2.47035

Abstract

Scabies is an infectious disease which is caused by Sarcoptes scabiei mites and considered as an emerging/re-emerging parasitic disease. Recent study using the Polymerase Chain Reaction (PCR) method has a higher sensitivity for detecting mites than using the skin scraping method. This study aims to detect the Second Internal Transcribed Spacer (ITS-2) encoding gene of S. scabiei. ITS-2 is a highly conserved area and always used to identify and differentiate host species infected by S. scabiei. S. scabiei isolates were obtained from skin scrapings from stray cat suspected of being infected with Scabies from Pasar Induk Bondowoso, Pasar Tamanan and Pasar Pujer. Detection on the presence of S. scabiei was carried out microscopically and then confirmed by molecular examination using the PCR method and visualized by electrophoresis on 2% agarose gel. The Polymerase Chain Reaction (PCR) results showed DNA bands at 304 bp Based on the result it can be concluded that samples are positively infected by Scabies can be detected by the ITS-2 encoding gene of Sarcoptes scabiei.
Molecular Detection of the Gene Encoding Second Internal Transcribed Spacer of Sarcoptes scabiei in Rabbits in Several Regions in East Java Rusdiana, Nur; Retno Lastuti, Nunuk Dyah; Aisyah, Siti; Purwanti, Ni Luh Lasmi; Sewoyo, Palagan Senopati
Jurnal Medika Veterinaria Vol 17, No 2 (2023): J.Med.Vet
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v17i2.35273

Abstract

This study aimed to detect the Second Internal Transcribed Spacer (ITS-2) encoding gene of Sarcoptes scabiei from rabbits. ITS-2 is a highly conserved gene and always used to identify and differentiate host species infected by S. scabiei. Isolates of S. scabieiwere collected from samples of rabbit skin scrapings suspected of being infected with S. scabiei. The rabbits were acquired from Surabaya, Sidoarjo, Pasuruan, Mojokerto and Nganjuk. Detection on the presence of S. scabiei was carried out microscopically then further confirmed by PCR method and visualized using electrophoresis. PCR identification results showed DNA bands at 304 base pairs. The results showed that samples were infected by S. scabiei, determined by the presence of ITS-2 encoding gene. The ITS-2 encoding gene was chosen as the main focus in this study because it was easily amplified that made analysis and identification easier. The high cases of scabies in Indonesia can be a warning to prevent infection of S. scabiei, especially in pets that have the potential as a reservoir in spreading diseases.
Co-Authors Amukti, Pandu Tokoh Aryaloka, Suhita Dadik Rahardjo, Dadik Hadi Warsito, Sunaryo Nadilla Chrisantya Yusuf Putri Nur Rusdiana Purwanti, Ni Luh Lasmi Sewoyo, Palagan Senopati Siti Aisyah Tri Suwanti, Lucia Wibawati, Prima Ayu