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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education)
Fazli, Rahmat Rizki
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Biochemistry, Genetics & Molecular Biology Education Medicine & Pharmacology Public Health

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The Study of Biosurfactant Stability and The Effect on Lipase Activity Alvionita, Mieke; Hertadi, Rukman; Fazli, Rahmat Rizki; Dewi, Andi Alfira Ratna Faradisa; Rose, Tasyang Oktavia
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.235-238

Abstract

Lipase is one of hydrolase enzyme that catalyzed hydrolysis reaction of triacylglycerols into free fatty acids and monoglycerides or glycerol. These biocatalysts are widely used in several industries, namely food and pharmaceutical industry. The activity of lipase can increase significantly if the substrate forms an emulsion. Since biosurfactant has been known to have emulsification characteristic, the effect of biosurfactant addition into lipase is necessary to be investigated. It is the first report that evaluate the effect of microbial surfactant on lipase activity. The purpose of this research is to analyze the stability of biosurfactant emulsion under various conditions, such as salinity and pH as well as their potential to enhance lipase activity. Biosurfactant used was Halomonas elongata BK-AG18 from the collection of Biochemistry Research Group ITB, Bandung, Indonesia. It was found that after the addition of NaCl, there was no significant decrease in the emulsification activity of the biosurfactant. The emulsification index (IE24) of biosurfactant with several NaCl concentrations (2, 5, 10, 15, 20, and 25%) was obtained around 50%. Stability test of biosurfactant at pH range 4-10 showed the highest IE24 of biosurfactant was obtained at pH 6. The effect of biosurfactants on lipase hydrolysis activity is also discussed in this article. Lipase hydrolysis activity was tested using p-nitrophenyl palmitate substrate. The highest lipase activity was obtained after the addition of 70% biosurfactant (v/v) at 0.026 units. This study shows that biosurfactant from H. elongata BK-AG18 has the potential to increase lipase activity.
Development of a Chemistry Module on Buffer Solutions Using Dual Representations Assisted by Animation Nasir*, Muhammad; Hasan, Muhammad; Adlim, Adlim; Fazli, Rahmat Rizki; Utari, Fitri Diah; Syukri, Muhammad; Khwaengmek, Wipavadee
Jurnal Pendidikan Sains Indonesia Vol 12, No 3 (2024): JULY 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jpsi.v12i3.38099

Abstract

Buffer solutions are a crucial concept in chemistry that students often find difficult to grasp. An effective method to enhance students' understanding is through the use of dual representations assisted by animation. Dual representations involve using various forms of visualization, such as diagrams, graphs, and animations, to help students comprehend abstract concepts more effectively. The research type employed is research and development (RD) using a 4-D model consisting of four stages: define, design, develop, and disseminate. The research subjects were 26 students from class XII MIPA 1 SMA Negeri 5 Banda Aceh. Data collection techniques were conducted by distributing questionnaires for student needs analysis, module and animation feasibility assessment, educator response to modules and animations, and student understanding tests. The developed module obtained very good assessment results from 2 validators with an average percentage of 89.16%, while the animation media received very good assessment results with an average percentage of 80.43%. Educators' responses to modules and animations were categorized as very good, with percentages of 94.17 and 95.84% respectively. Students' responses were also categorized as very good with an average percentage of 87.2%. The results of students' understanding were categorized as good with an overall score of 82.60%. Based on the results of this research, it is concluded that the animation-assisted multiple representation module on buffer solution material is highly suitable for use as teaching material in the learning process
Co-Authors Adlim Adlim Alvionita, Mieke Dewi, Andi Alfira Ratna Faradisa Khwaengmek, Wipavadee Muhammad Hasan Muhammad Syukri Nasir*, Muhammad Rose, Tasyang Oktavia RUKMAN HERTADI Utari, Fitri Diah