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All Journal Industria: Jurnal Teknologi dan Manajemen Agroindustri Jurnal Riset Biologi dan Aplikasinya
Jamil, Nazia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Other

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Micrococcus yunnanensis and Psychrobacter sp. as Potential Producers of Polymers from Hot Spring Jabeen, Asma; Batool, Rida; Jamil, Nazia
Industria: Jurnal Teknologi dan Manajemen Agroindustri Vol 11, No 1 (2022)
Publisher : Department of Agro-industrial Technology, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.industria.2022.011.01.1

Abstract

AbstractPolyhydroxyalkanoates (PHAs) and exopolysaccharides (EPSs) are biopolymers bacteria under nutrient-limiting conditions. In this study, bacterial strains were isolated from hot springs. Soil samples were collected from Tatta Pani, Azad Kashmir, Pakistan. Bacterial strains AJ2 and AJ3 were selected due to their ability to produce PHAs and EPSs. Phylogenetic analysis showed that strain AJ2 was Micrococcus yunnanensis and AJ3 was Psychrobacter sp. Three carbon sources (glucose, glycerol, and molasses) were used for polymer production. The effect of high pH (8) and high temperature (55 °C) was checked on PHAs and EPSs production. The highest yield of PHAs was given by strain AJ3 (89.43%) with molasses. When grown at 55 °C for 24 hours, strain AJ3 showed the highest PHAs accumulation, 79% with glucose. At alkaline pH 8, strain AJ3 gave 34% PHAs with molasses. The highest EPSs production was observed for strain AJ3. AJ3 gave 70g/L of EPSs with both glucose and glycerol. The amplification of the phaC gene was done to confirm the genetic basis of PHAs production. FTIR analysis showed clear bands at 1722 cm-1 and 2925 cm-1 representing the carbonyl and alkyl groups of PHAs, respectively.Keywords: exopolysaccharides, Kashmir, Micrococcus yunnanensis, polyhydroxyalkanoates, Psychrobacter sp. AbstrakPolihidroksialkanoat (PHA) dan eksopolisakarida (EPS) adalah biopolimer yang diproduksi oleh bakteri yang hidup pada kondisi nutrisi yang terbatas. Dalam penelitian ini, strain bakteri diisolasi dari sumber air panas. Sampel tanah dikumpulkan dari Tatta Pani, Azad Kashmir, Pakistan. Strain bakteri AJ2 dan AJ3 dipilih karena kemampuannya menghasilkan PHA dan EPS. Analisis filogenetik menunjukkan bahwa strain AJ2 adalah Micrococcus yunnanensis dan AJ3 adalah Psychrobacter sp. Tiga sumber karbon (glukosa, gliserol, dan molase) digunakan untuk produksi polimer. pH tinggi (8) dan suhu tinggi (55 °C) diperiksa pengaruhnya terhadap produksi PHA dan EPS. Hasil tertinggi PHA diberikan oleh strain AJ3 (89,43%) dengan tetes tebu. Saat ditumbuhkan pada suhu 55 °C selama 24 jam, strain AJ3 menunjukkan akumulasi PHA tertinggi, 79% dengan glukosa. Pada pH basa 8, strain AJ3 memberikan 34% PHA dengan molase. Produksi EPS tertinggi diamati untuk strain AJ3. AJ3 menghasilkan 70g/L EPS dengan glukosa dan gliserol. Amplifikasi gen phaC dilakukan untuk mengkonfirmasi dasar genetik produksi PHA. Analisis FTIR menunjukkan pita yang jelas pada 1722 cm-1 dan 2925 cm-1 masing-masing mewakili gugus karbonil dan alkil PHA.Kata kunci: eksopolisakarida, Kashmir, Micrococcus yunnanensis, polihidroksialkanoat, Psychrobacter sp.
Characterization and Molecular Identification of Lipolytic-bacterial Isolates Forming Biofilm on Polyethylene Plastic Ariyanti, Olivia Puja; Agustina, Putri; Anif, Sofyan; Seno, Hernandito Aryo; Hardianto, Alden Ganendra Madhava Priya; Mandiri, Listy Hasti; Zulperi, Dzarifah; Jamil, Nazia; Rahayu, Triastuti; Sidiq, Yasir
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p57-65

Abstract

Polyethylene (PE) plastic is one of the most widely used for multiple purposes and leading the environmental problems. Lipolytic bacteria are promising agents to reduce the plastic waste. The dry weight of PE plastic was reduced by 33 % in the Winogradsky's column after 45 days of incubation. However, the lipolytic bacteria responsible for those reduction was unknown. This study aimed to characterize and identify the potential lipolytic bacterial isolates forming biofilm on polyethylene PE plastics. Samples of lipolytic bacterial isolates were screened on tributyrin selective media based on the formed clear zone. Moreover, the 16S rRNA genes of the two most potential lipolytic bacterial isolates were amplified. Then, the amplicons of the 16S rRNA gene were sequenced. This study found two potential lipolytic bacteria isolates, AB A-2 and AB M-3, which had the characteristics of round colonies, wavy edges, convex surfaces, and milky white color. The two isolates are gram-positive and have the shape of Coccobacillus cells. The molecular identification showed that AB A-2 isolate was Bacillus sp., while AB M-3 isolate was Bacillus amyloliquefaciens. This finding contributes to novel bacterial isolates that potentially overcoming the plastic waste problem.
Co-Authors Ariyanti, Olivia Puja Batool, Rida Hardianto, Alden Ganendra Madhava Priya Jabeen, Asma Mandiri, Listy Hasti Putri Agustina Seno, Hernandito Aryo Sofyan Anif Triastuti Rahayu Yasir Sidiq Zulperi, Dzarifah